Restriction map for
pF1KE0035
(Circular) (Six-base) MAPSORT of: pF1KE0035.seq Check: 4472 from: 1 to: 4121
pF1KE0035 4121 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1159 1159
Size: 4121
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 1946 2378 3125 3393 587
Size: 1359 432 747 268 1315
Fragments arranged by size:
1359 1315 747 432 268
AflIII A'CryG_T
Cuts at: 1473 2577 3554 1473
Size: 1104 977 2040
Fragments arranged by size:
2040 1104 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 201 409 1426 2993 201
Size: 208 1017 1567 1329
Fragments arranged by size:
1567 1329 1017 208
ApaI G_GGCC'C
Cuts at: 188 188
Size: 4121
ApaLI G'TGCA_C
Cuts at: 2891 2891
Size: 4121
ApoI r'AATT_y
Cuts at: 1125 3474 3730 1125
Size: 2349 256 1516
Fragments arranged by size:
2349 1516 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 1141 1141
Size: 4121
AvrII C'CTAG_G
Cuts at: 508 508
Size: 4121
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 952 1137 1551 1799 1834 952
Size: 185 414 248 35 3239
Fragments arranged by size:
3239 414 248 185 35
BanII G_rGCy'C
Cuts at: 188 380 434 1135 2165 188
Size: 192 54 701 1030 2144
Fragments arranged by size:
2144 1030 701 192 54
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 244 568 244
Size: 324 3797
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 248 317 524 1641 248
Size: 69 207 1117 2728
Fragments arranged by size:
2728 1117 207 69
BglI GCCn_nnn'nGGC
Cuts at: 124 352 124
Size: 228 3893
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 188 553 611 957 1746 1839 2895 188
Size: 365 58 346 789 93 1056 1414
Fragments arranged by size:
1414 1056 789 365 346 93 58
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1414 1749 3893 1333
Size: 81 335 2144 1561
Fragments arranged by size:
2144 1561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 926 481
Size: 445 3676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 413 1552 1800 2502 3998 413
Size: 1139 248 702 1496 536
Fragments arranged by size:
1496 1139 702 536 248
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 299 329 299
Size: 30 4091
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 631 364
Size: 267 3854
BseYI C'CCAG_C
Cuts at: 658 1546 2304 2881 3377 658
Size: 888 758 577 496 1402
Fragments arranged by size:
1402 888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 287 1663 1709 2917 104
Size: 183 1376 46 1208 1308
Fragments arranged by size:
1376 1308 1208 183 46
BsiHKAI G_wGCw'C
Cuts at: 380 434 1135 1913 2103 2895 380
Size: 54 701 778 190 792 1606
Fragments arranged by size:
1606 792 778 701 190 54
Bsp1286I G_dGCh'C
Cuts at: 188 380 434 553 611 957 1135 1746 1839
Size: 192 54 119 58 346 178 611 93
Cuts at: 1839 1913 2103 2165 2895 188
Size: 74 190 62 730 1414
Fragments arranged by size:
1414 730 611 346 192 190 178 119
93 74 62 58 54
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 1385 1687 2068 1171
Size: 214 302 381 3224
Fragments arranged by size:
3224 381 302 214
BsrBI CCG'CTC
Cuts at: 50 142 2413 2467 3951 4116 50
Size: 92 2271 54 1484 165 55
Fragments arranged by size:
2271 1484 165 92 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 173 2119 2300 2509 173
Size: 1946 181 209 1785
Fragments arranged by size:
1946 1785 209 181
BssHII G'CGCG_C
Cuts at: 338 340 342 474 338
Size: 2 2 132 3985
Fragments arranged by size:
3985 132 2 2
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstXI CCAn_nnnn'nTGG
Cuts at: 114 972 1053 114
Size: 858 81 3182
Fragments arranged by size:
3182 858 81
BstYI r'GATC_y
Cuts at: 1 539 1146 1613 1636 1971 2217 3229 3315
Size: 538 607 467 23 335 246 1012 86
Cuts at: 3315 3327 3673 1
Size: 12 346 449
Fragments arranged by size:
1012 607 538 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 986 986
Size: 4121
BtgI C'CryG_G
Cuts at: 107 281 389 107
Size: 174 108 3839
Fragments arranged by size:
3839 174 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 119 1364 119
Size: 1245 2876
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 116 137 284 1706 1880 2271 2298 3643 116
Size: 21 147 1422 174 391 27 1345 594
Fragments arranged by size:
1422 1345 594 391 174 147 27 21
EagI C'GGCC_G
Cuts at: 284 1706 284
Size: 1422 2699
EarI CTCTTCn'nnn_
Cuts at: 497 913 984 2144 2354 497
Size: 416 71 1160 210 2264
Fragments arranged by size:
2264 1160 416 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 370 2329 2639 2785 3931 358
Size: 12 1959 310 146 1146 548
Fragments arranged by size:
1959 1146 548 310 146 12
EcoICRI GAG'CTC
Cuts at: 378 432 1133 378
Size: 54 701 3366
Fragments arranged by size:
3366 701 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 587 926 1946 2378 3125 3393 481
Size: 106 339 1020 432 747 268 1209
Fragments arranged by size:
1209 1020 747 432 339 268 106
EcoO109I rG'GnC_Cy
Cuts at: 184 216 1260 184
Size: 32 1044 3045
Fragments arranged by size:
3045 1044 32
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
EcoRV GAT'ATC
Cuts at: 1086 1086
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 4121
FspI TGC'GCA
Cuts at: 205 1902 205
Size: 1697 2424
HaeII r_GCGC'y
Cuts at: 157 1555 1803 2825 3804 157
Size: 1398 248 1022 979 474
Fragments arranged by size:
1398 1022 979 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 442 474 1963 1993 1995 2025 442
Size: 32 1489 30 2 30 2538
Fragments arranged by size:
2538 1489 32 30 30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
HindIII A'AGCT_T
Cuts at: 747 747
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2792 2976 2792
Size: 184 3937
MscI TGG'CCA
Cuts at: 118 1882 118
Size: 1764 2357
MslI CAynn'nnrTG
Cuts at: 112 2110 2237 112
Size: 1998 127 1996
Fragments arranged by size:
1998 1996 127
MspA1I CmG'CkG
Cuts at: 198 266 391 419 1232 1546 1906 2919 3164
Size: 68 125 28 813 314 360 1013 245
Cuts at: 3164 198
Size: 1155
Fragments arranged by size:
1155 1013 813 360 314 245 125 68
28
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 107 107
Size: 4121
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NspI r_CATG'y
Cuts at: 1174 1674 2581 1174
Size: 500 907 2714
Fragments arranged by size:
2714 907 500
PasI CC'CwG_GG
Cuts at: 188 188
Size: 4121
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PflMI CCAn_nnn'nTGG
Cuts at: 409 409
Size: 4121
PfoI T'CCnGG_A
Cuts at: 440 497 440
Size: 57 4064
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 571 603 3260 3292 571
Size: 32 2657 32 1400
Fragments arranged by size:
2657 1400 32 32
PpuMI rG'GwC_Cy
Cuts at: 216 216
Size: 4121
PsiI TTA'TAA
Cuts at: 692 692
Size: 4121
PspOMI G'GGCC_C
Cuts at: 184 184
Size: 4121
PstI C_TGCA'G
Cuts at: 1168 1853 1168
Size: 685 3436
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 1546 1906 1546
Size: 360 3761
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 380 434 1135 380
Size: 54 701 3366
Fragments arranged by size:
3366 701 54
SacII CC_GC'GG
Cuts at: 392 392
Size: 4121
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SanDI GG'GwC_CC
Cuts at: 216 216
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 497 2144 2354 497
Size: 1647 210 2264
Fragments arranged by size:
2264 1647 210
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 134 3638 134
Size: 3504 617
SfcI C'TryA_G
Cuts at: 33 1164 1849 2842 3033 33
Size: 1131 685 993 191 1121
Fragments arranged by size:
1131 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
StyI C'CwwG_G
Cuts at: 107 508 633 1255 1609 107
Size: 401 125 622 354 2619
Fragments arranged by size:
2619 622 401 354 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 1837 2296 2505 1704
Size: 133 459 209 3320
Fragments arranged by size:
3320 459 209 133
TatI w'GTAC_w
Cuts at: 132 3636 132
Size: 3504 617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 3638 3670 299
Size: 32 3307 32 750
Fragments arranged by size:
3307 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 666 1616 666
Size: 950 3171
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI