Restriction map for
pF1KE0033
(Circular) (Six-base) MAPSORT of: pF1KE0033.seq Check: 1151 from: 1 to: 4805
pF1KE0033 4805 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1758 1758
Size: 4805
AccI GT'mk_AC
Cuts at: 1843 1843
Size: 4805
Acc65I G'GTAC_C
Cuts at: 1821 1821
Size: 4805
AclI AA'CG_TT
Cuts at: 4645 4645
Size: 4805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 2630 3062 3809 4077 326
Size: 2304 432 747 268 1054
Fragments arranged by size:
2304 1054 747 432 268
AfeI AGC'GCT
Cuts at: 162 162
Size: 4805
AflIII A'CryG_T
Cuts at: 1779 2157 3261 4238 1779
Size: 378 1104 977 2346
Fragments arranged by size:
2346 1104 977 378
AgeI A'CCGG_T
Cuts at: 3193 3193
Size: 4805
AleI CACnn'nnGTG
Cuts at: 181 181
Size: 4805
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 617 617
Size: 4805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1142 1174 1142
Size: 32 4773
AlwNI CAG_nnn'CTG
Cuts at: 2110 3677 2110
Size: 1567 3238
ApaI G_GGCC'C
Cuts at: 1343 1343
Size: 4805
ApaLI G'TGCA_C
Cuts at: 3575 3575
Size: 4805
ApoI r'AATT_y
Cuts at: 518 847 1240 1531 1809 4158 4414 518
Size: 329 393 291 278 2349 256 909
Fragments arranged by size:
2349 909 393 329 291 278 256
AseI AT'TA_AT
Cuts at: 20 1364 20
Size: 1344 3461
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4805
AvaI C'yCGr_G
Cuts at: 1730 1825 1730
Size: 95 4710
BamHI G'GATC_C
Cuts at: 1830 1830
Size: 4805
BanI G'GyrC_C
Cuts at: 1821 2235 2483 2518 1821
Size: 414 248 35 4108
Fragments arranged by size:
4108 414 248 35
BanII G_rGCy'C
Cuts at: 421 1343 1731 1819 2849 421
Size: 922 388 88 1030 2377
Fragments arranged by size:
2377 1030 922 388 88
BbeI G_GCGC'C
Cuts at: 2239 2487 2239
Size: 248 4557
BciVI GTATCCnnnnn_n'
Cuts at: 2703 3464 4805 2703
Size: 761 1341 2703
Fragments arranged by size:
2703 1341 761
BclI T'GATC_A
Cuts at: 2325 2325
Size: 4805
BglI GCCn_nnn'nGGC
Cuts at: 172 172
Size: 4805
BglII A'GATC_T
Cuts at: 749 2320 4357 749
Size: 1571 2037 1197
Fragments arranged by size:
2037 1571 1197
BlpI GC'TnA_GC
Cuts at: 157 1079 1917 157
Size: 922 838 3045
Fragments arranged by size:
3045 922 838
Bme1580I G_kGCm'C
Cuts at: 1343 2430 2523 3579 1343
Size: 1087 93 1056 2569
Fragments arranged by size:
2569 1087 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 822 1401 2017 2098 2433 4577 822
Size: 579 616 81 335 2144 1050
Fragments arranged by size:
2144 1050 616 579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3172 3172
Size: 4805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1202
Size: 4805
Bpu10I CC'TnA_GC
Cuts at: 1703 1703
Size: 4805
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1981 2860 3352 3650 1981
Size: 879 492 298 3136
Fragments arranged by size:
3136 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4509 4509
Size: 4805
BsaAI yAC'GTr
Cuts at: 2158 2158
Size: 4805
BsaBI GATnn'nnATC
Cuts at: 6 2319 4356 6
Size: 2313 2037 455
Fragments arranged by size:
2313 2037 455
BsaHI Gr'CG_yC
Cuts at: 2236 2484 3186 4682 2236
Size: 248 702 1496 2359
Fragments arranged by size:
2359 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1994 2515 3193 3467 3614 4025 1994
Size: 521 678 274 147 411 2774
Fragments arranged by size:
2774 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 461 491 1142 1172 1550 1580 461
Size: 30 651 30 378 30 3686
Fragments arranged by size:
3686 651 378 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1566 1566
Size: 4805
BseYI C'CCAG_C
Cuts at: 2230 2988 3565 4061 2230
Size: 758 577 496 2974
Fragments arranged by size:
2974 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 1630
Size: 4805
BsiEI CG_ry'CG
Cuts at: 104 2347 2393 3601 104
Size: 2243 46 1208 1308
Fragments arranged by size:
2243 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1819 2597 2787 3579 1819
Size: 778 190 792 3045
Fragments arranged by size:
3045 792 778 190
BsmI GAATG_Cn'
Cuts at: 742 1125 742
Size: 383 4422
Bsp1286I G_dGCh'C
Cuts at: 421 1343 1731 1819 2430 2523 2597 2787 2849
Size: 922 388 88 611 93 74 190 62
Cuts at: 2849 3579 421
Size: 730 1647
Fragments arranged by size:
1647 922 730 611 388 190 93 88
74 62
BspEI T'CCGG_A
Cuts at: 4025 4025
Size: 4805
BspHI T'CATG_A
Cuts at: 831 3981 831
Size: 3150 1655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1758 1855 2069 2371 2752 1758
Size: 97 214 302 381 3811
Fragments arranged by size:
3811 381 302 214 97
BsrBI CCG'CTC
Cuts at: 50 3097 3151 4635 4800 50
Size: 3047 54 1484 165 55
Fragments arranged by size:
3047 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1547 2717 1547
Size: 1170 3635
BsrFI r'CCGG_y
Cuts at: 2803 2984 3193 2803
Size: 181 209 4415
Fragments arranged by size:
4415 209 181
BsrGI T'GTAC_A
Cuts at: 261 460 1326 261
Size: 199 866 3740
Fragments arranged by size:
3740 866 199
BssSI C'ACGA_G
Cuts at: 3076 3434 3076
Size: 358 4447
BstAPI GCAn_nnn'nTGC
Cuts at: 1381 1617 2182 1381
Size: 236 565 4004
Fragments arranged by size:
4004 565 236
BstBI TT'CG_AA
Cuts at: 3166 3166
Size: 4805
BstXI CCAn_nnnn'nTGG
Cuts at: 183 183
Size: 4805
BstYI r'GATC_y
Cuts at: 1 642 749 1697 1830 2297 2320 2655 2901
Size: 641 107 948 133 467 23 335 246
Cuts at: 2901 3913 3999 4011 4357 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 948 641 467 449 346 335 246
133 107 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4805
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2755 2936 3099 2755
Size: 181 163 4461
Fragments arranged by size:
4461 181 163
BtsI GCAGTG_nn'
Cuts at: 128 1151 2048 128
Size: 1023 897 2885
Fragments arranged by size:
2885 1023 897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 1332
Size: 35 4770
DraI TTT'AAA
Cuts at: 326 1804 326
Size: 1478 3327
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2511 3369 1467
Size: 1044 858 2903
Fragments arranged by size:
2903 1044 858
EaeI y'GGCC_r
Cuts at: 173 2390 2564 2955 2982 4327 173
Size: 2217 174 391 27 1345 651
Fragments arranged by size:
2217 1345 651 391 174 27
EagI C'GGCC_G
Cuts at: 2390 2390
Size: 4805
EarI CTCTTCn'nnn_
Cuts at: 2828 3038 2828
Size: 210 4595
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3013 3323 3469 4615 3013
Size: 310 146 1146 3203
Fragments arranged by size:
3203 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1817 1817
Size: 4805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1202 2630 3062 3809 4077 326
Size: 876 1428 432 747 268 1054
Fragments arranged by size:
1428 1054 876 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1944 1944
Size: 4805
EcoRI G'AATT_C
Cuts at: 518 1809 518
Size: 1291 3514
EcoRV GAT'ATC
Cuts at: 1232 1232
Size: 4805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 642 645 1400 2824 642
Size: 3 755 1424 2623
Fragments arranged by size:
2623 1424 755 3
FspI TGC'GCA
Cuts at: 2586 2586
Size: 4805
HaeII r_GCGC'y
Cuts at: 164 2239 2487 3509 4488 164
Size: 2075 248 1022 979 481
Fragments arranged by size:
2075 1022 979 481 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2647 2677 2679 2709 2647
Size: 30 2 30 4743
Fragments arranged by size:
4743 30 30 2
HincII GTy'rAC
Cuts at: 1844 1844
Size: 4805
KasI G'GCGC_C
Cuts at: 2235 2483 2235
Size: 248 4557
KpnI G_GTAC'C
Cuts at: 1825 1825
Size: 4805
MluI A'CGCG_T
Cuts at: 4238 4238
Size: 4805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 252 1500 3476 3660 252
Size: 1248 1976 184 1397
Fragments arranged by size:
1976 1397 1248 184
MscI TGG'CCA
Cuts at: 2566 2566
Size: 4805
MslI CAynn'nnrTG
Cuts at: 181 2794 2921 181
Size: 2613 127 2065
Fragments arranged by size:
2613 2065 127
MspA1I CmG'CkG
Cuts at: 156 1916 2230 2590 3603 3848 156
Size: 1760 314 360 1013 245 1113
Fragments arranged by size:
1760 1113 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2986 2986
Size: 4805
NarI GG'CG_CC
Cuts at: 2236 2484 2236
Size: 248 4557
NcoI C'CATG_G
Cuts at: 107 107
Size: 4805
NgoMIV G'CCGG_C
Cuts at: 2984 2984
Size: 4805
NspI r_CATG'y
Cuts at: 1783 1858 2358 3265 1783
Size: 75 500 907 3323
Fragments arranged by size:
3323 907 500 75
PciI A'CATG_T
Cuts at: 1779 3261 1779
Size: 1482 3323
PmeI GTTT'AAAC
Cuts at: 1804 1804
Size: 4805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1142 1174 3944 3976 1142
Size: 32 2770 32 1971
Fragments arranged by size:
2770 1971 32 32
PspOMI G'GGCC_C
Cuts at: 1339 1339
Size: 4805
PstI C_TGCA'G
Cuts at: 205 1852 2537 205
Size: 1647 685 2473
Fragments arranged by size:
2473 1647 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4805
PvuII CAG'CTG
Cuts at: 156 2230 2590 156
Size: 2074 360 2371
Fragments arranged by size:
2371 2074 360
RsrII CG'GwC_CG
Cuts at: 3000 3000
Size: 4805
SacI G_AGCT'C
Cuts at: 1819 1819
Size: 4805
SalI G'TCGA_C
Cuts at: 1842 1842
Size: 4805
SapI GCTCTTCn'nnn_
Cuts at: 2828 3038 2828
Size: 210 4595
SbfI CC_TGCA'GG
Cuts at: 1852 1852
Size: 4805
ScaI AGT'ACT
Cuts at: 4322 4322
Size: 4805
SfcI C'TryA_G
Cuts at: 33 201 861 1848 2533 3526 3717 33
Size: 168 660 987 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 660 191 168
SfoI GGC'GCC
Cuts at: 2237 2485 2237
Size: 248 4557
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4805
SmaI CCC'GGG
Cuts at: 1827 1827
Size: 4805
SmlI C'TyrA_G
Cuts at: 1960 2875 3367 3629 1960
Size: 915 492 262 3136
Fragments arranged by size:
3136 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4805
SphI G_CATG'C
Cuts at: 1858 2358 1858
Size: 500 4305
StyI C'CwwG_G
Cuts at: 107 1939 2293 107
Size: 1832 354 2619
Fragments arranged by size:
2619 1832 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 833 2388 2521 2980 3189 833
Size: 1555 133 459 209 2449
Fragments arranged by size:
2449 1555 459 209 133
TatI w'GTAC_w
Cuts at: 261 460 1326 4320 261
Size: 199 866 2994 746
Fragments arranged by size:
2994 866 746 199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 519 551 711 743 4322 4354 519
Size: 32 160 32 3579 32 970
Fragments arranged by size:
3579 970 160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2602 2602
Size: 4805
XbaI T'CTAG_A
Cuts at: 874 1579 1836 874
Size: 705 257 3843
Fragments arranged by size:
3843 705 257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2300 2300
Size: 4805
XmaI C'CCGG_G
Cuts at: 1825 1825
Size: 4805
XmnI GAAnn'nnTTC
Cuts at: 625 1157 625
Size: 532 4273
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI