Restriction map for
pF1KE0031
(Circular) (Six-base) MAPSORT of: pF1KE0031.seq Check: 389 from: 1 to: 4595
pF1KE0031 4595 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1169 1633 1169
Size: 464 4131
Acc65I G'GTAC_C
Cuts at: 1611 1611
Size: 4595
AclI AA'CG_TT
Cuts at: 4435 4435
Size: 4595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1527 2420 2852 3599 3867 1527
Size: 893 432 747 268 2255
Fragments arranged by size:
2255 893 747 432 268
AflIII A'CryG_T
Cuts at: 1947 3051 4028 1947
Size: 1104 977 2514
Fragments arranged by size:
2514 1104 977
AgeI A'CCGG_T
Cuts at: 2983 2983
Size: 4595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1372 1404 1372
Size: 32 4563
AlwNI CAG_nnn'CTG
Cuts at: 1900 3467 1900
Size: 1567 3028
ApaLI G'TGCA_C
Cuts at: 3365 3365
Size: 4595
ApoI r'AATT_y
Cuts at: 616 1569 1599 3948 4204 616
Size: 953 30 2349 256 1007
Fragments arranged by size:
2349 1007 953 256 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4595
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
AvaI C'yCGr_G
Cuts at: 1615 1615
Size: 4595
BamHI G'GATC_C
Cuts at: 1620 1620
Size: 4595
BanI G'GyrC_C
Cuts at: 1611 2025 2273 2308 1611
Size: 414 248 35 3898
Fragments arranged by size:
3898 414 248 35
BanII G_rGCy'C
Cuts at: 474 1113 1609 2639 474
Size: 639 496 1030 2430
Fragments arranged by size:
2430 1030 639 496
BbeI G_GCGC'C
Cuts at: 2029 2277 2029
Size: 248 4347
BbsI GAAGACnn'nnnn_
Cuts at: 1058 1058
Size: 4595
BbvCI CC'TCA_GC
Cuts at: 1361 1361
Size: 4595
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 743 777 743
Size: 34 4561
BciVI GTATCCnnnnn_n'
Cuts at: 2493 3254 4595 2493
Size: 761 1341 2493
Fragments arranged by size:
2493 1341 761
BclI T'GATC_A
Cuts at: 2115 2115
Size: 4595
BfrBI ATG'CAT
Cuts at: 217 217
Size: 4595
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4595
BglII A'GATC_T
Cuts at: 2110 4147 2110
Size: 2037 2558
BlpI GC'TnA_GC
Cuts at: 1707 1707
Size: 4595
Bme1580I G_kGCm'C
Cuts at: 612 2220 2313 3369 612
Size: 1608 93 1056 1838
Fragments arranged by size:
1838 1608 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1807 1888 2223 4367 1807
Size: 81 335 2144 2035
Fragments arranged by size:
2144 2035 335 81
BmtI G_CTAG'C
Cuts at: 296 296
Size: 4595
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1127 2962 1127
Size: 1835 2760
Bpu10I CC'TnA_GC
Cuts at: 497 1027 1361 497
Size: 530 334 3731
Fragments arranged by size:
3731 530 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1122 1771 2650 3142 3440 634
Size: 488 649 879 492 298 1789
Fragments arranged by size:
1789 879 649 492 488 298
BsaI GGTCTCn'nnnn_
Cuts at: 4299 4299
Size: 4595
BsaAI yAC'GTr
Cuts at: 1948 1948
Size: 4595
BsaBI GATnn'nnATC
Cuts at: 6 1496 2109 4146 6
Size: 1490 613 2037 455
Fragments arranged by size:
2037 1490 613 455
BsaHI Gr'CG_yC
Cuts at: 2026 2274 2976 4472 2026
Size: 248 702 1496 2149
Fragments arranged by size:
2149 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1784 2305 2983 3257 3404 3815 1784
Size: 521 678 274 147 411 2564
Fragments arranged by size:
2564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1372
Size: 30 4565
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1390 1393 1486 1390
Size: 3 93 4499
Fragments arranged by size:
4499 93 3
BseYI C'CCAG_C
Cuts at: 672 2020 2778 3355 3851 672
Size: 1348 758 577 496 1416
Fragments arranged by size:
1416 1348 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1041 1041
Size: 4595
BsiEI CG_ry'CG
Cuts at: 104 149 2137 2183 3391 104
Size: 45 1988 46 1208 1308
Fragments arranged by size:
1988 1308 1208 46 45
BsiHKAI G_wGCw'C
Cuts at: 1113 1609 2387 2577 3369 1113
Size: 496 778 190 792 2339
Fragments arranged by size:
2339 792 778 496 190
BsmI GAATG_Cn'
Cuts at: 444 525 1284 1519 444
Size: 81 759 235 3520
Fragments arranged by size:
3520 759 235 81
Bsp1286I G_dGCh'C
Cuts at: 474 612 1113 1609 2220 2313 2387 2577 2639
Size: 138 501 496 611 93 74 190 62
Cuts at: 2639 3369 474
Size: 730 1700
Fragments arranged by size:
1700 730 611 501 496 190 138 93
74 62
BspEI T'CCGG_A
Cuts at: 3815 3815
Size: 4595
BspHI T'CATG_A
Cuts at: 3771 3771
Size: 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1645 1859 2161 2542 1645
Size: 214 302 381 3698
Fragments arranged by size:
3698 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2887 2941 4425 4590 50
Size: 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 674 848 2507 674
Size: 174 1659 2762
Fragments arranged by size:
2762 1659 174
BsrFI r'CCGG_y
Cuts at: 2593 2774 2983 2593
Size: 181 209 4205
Fragments arranged by size:
4205 209 181
BsrGI T'GTAC_A
Cuts at: 941 941
Size: 4595
BssSI C'ACGA_G
Cuts at: 835 2866 3224 835
Size: 2031 358 2206
Fragments arranged by size:
2206 2031 358
BstAPI GCAn_nnn'nTGC
Cuts at: 223 854 1972 223
Size: 631 1118 2846
Fragments arranged by size:
2846 1118 631
BstBI TT'CG_AA
Cuts at: 2956 2956
Size: 4595
BstYI r'GATC_y
Cuts at: 1 1620 2087 2110 2445 2691 3703 3789 3801
Size: 1619 467 23 335 246 1012 86 12
Cuts at: 3801 4147 1
Size: 346 449
Fragments arranged by size:
1619 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1170 1170
Size: 4595
BtgI C'CryG_G
Cuts at: 107 119 443 107
Size: 12 324 4259
Fragments arranged by size:
4259 324 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 143 619 2545 2726 2889 143
Size: 476 1926 181 163 1849
Fragments arranged by size:
1926 1849 476 181 163
BtsI GCAGTG_nn'
Cuts at: 1073 1838 1073
Size: 765 3830
DraI TTT'AAA
Cuts at: 1594 1594
Size: 4595
DrdI GACnn_nn'nnGTC
Cuts at: 391 2301 3159 391
Size: 1910 858 1827
Fragments arranged by size:
1910 1827 858
EaeI y'GGCC_r
Cuts at: 2180 2354 2745 2772 4117 2180
Size: 174 391 27 1345 2658
Fragments arranged by size:
2658 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2180 2180
Size: 4595
EarI CTCTTCn'nnn_
Cuts at: 175 1101 1228 2618 2828 175
Size: 926 127 1390 210 1942
Fragments arranged by size:
1942 1390 926 210 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2803 3113 3259 4405 2803
Size: 310 146 1146 2993
Fragments arranged by size:
2993 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1111 1607 1111
Size: 496 4099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1527 2420 2852 3599 3867 1527
Size: 893 432 747 268 2255
Fragments arranged by size:
2255 893 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1468 1468
Size: 4595
EcoO109I rG'GnC_Cy
Cuts at: 1461 1734 1461
Size: 273 4322
EcoRI G'AATT_C
Cuts at: 1599 1599
Size: 4595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1539 2614 1539
Size: 1075 3520
FspI TGC'GCA
Cuts at: 1217 2376 1217
Size: 1159 3436
HaeII r_GCGC'y
Cuts at: 2029 2277 3299 4278 2029
Size: 248 1022 979 2346
Fragments arranged by size:
2346 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1317 1349 2437 2467 2469 2499 1317
Size: 32 1088 30 2 30 3413
Fragments arranged by size:
3413 1088 32 30 30 2
HincII GTy'rAC
Cuts at: 1634 1634
Size: 4595
HindIII A'AGCT_T
Cuts at: 245 1024 1072 1304 245
Size: 779 48 232 3536
Fragments arranged by size:
3536 779 232 48
KasI G'GCGC_C
Cuts at: 2025 2273 2025
Size: 248 4347
KpnI G_GTAC'C
Cuts at: 1615 1615
Size: 4595
MluI A'CGCG_T
Cuts at: 4028 4028
Size: 4595
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 359 1380 3266 3450 359
Size: 1021 1886 184 1504
Fragments arranged by size:
1886 1504 1021 184
MscI TGG'CCA
Cuts at: 2356 2356
Size: 4595
MslI CAynn'nnrTG
Cuts at: 274 322 491 1483 2584 2711 274
Size: 48 169 992 1101 127 2158
Fragments arranged by size:
2158 1101 992 169 127 48
MspA1I CmG'CkG
Cuts at: 121 1221 1706 2020 2380 3393 3638 121
Size: 1100 485 314 360 1013 245 1078
Fragments arranged by size:
1100 1078 1013 485 360 314 245
NaeI GCC'GGC
Cuts at: 2776 2776
Size: 4595
NarI GG'CG_CC
Cuts at: 2026 2274 2026
Size: 248 4347
NcoI C'CATG_G
Cuts at: 107 443 107
Size: 336 4259
NdeI CA'TA_TG
Cuts at: 933 933
Size: 4595
NgoMIV G'CCGG_C
Cuts at: 2774 2774
Size: 4595
NheI G'CTAG_C
Cuts at: 292 292
Size: 4595
NsiI A_TGCA'T
Cuts at: 219 219
Size: 4595
NspI r_CATG'y
Cuts at: 455 1648 2148 3055 455
Size: 1193 500 907 1995
Fragments arranged by size:
1995 1193 907 500
PasI CC'CwG_GG
Cuts at: 954 954
Size: 4595
PciI A'CATG_T
Cuts at: 3051 3051
Size: 4595
PfoI T'CCnGG_A
Cuts at: 341 341
Size: 4595
PmeI GTTT'AAAC
Cuts at: 1594 1594
Size: 4595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1372 1404 3734 3766 1372
Size: 32 2330 32 2201
Fragments arranged by size:
2330 2201 32 32
PpuMI rG'GwC_Cy
Cuts at: 1461 1461
Size: 4595
PsiI TTA'TAA
Cuts at: 251 251
Size: 4595
PstI C_TGCA'G
Cuts at: 859 1642 2327 859
Size: 783 685 3127
Fragments arranged by size:
3127 783 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4595
PvuII CAG'CTG
Cuts at: 1221 2020 2380 1221
Size: 799 360 3436
Fragments arranged by size:
3436 799 360
RsrII CG'GwC_CG
Cuts at: 2790 2790
Size: 4595
SacI G_AGCT'C
Cuts at: 1113 1609 1113
Size: 496 4099
SacII CC_GC'GG
Cuts at: 122 122
Size: 4595
SalI G'TCGA_C
Cuts at: 1632 1632
Size: 4595
SapI GCTCTTCn'nnn_
Cuts at: 1101 2618 2828 1101
Size: 1517 210 2868
Fragments arranged by size:
2868 1517 210
SbfI CC_TGCA'GG
Cuts at: 1642 1642
Size: 4595
ScaI AGT'ACT
Cuts at: 4112 4112
Size: 4595
SfcI C'TryA_G
Cuts at: 33 855 1638 2323 3316 3507 33
Size: 822 783 685 993 191 1121
Fragments arranged by size:
1121 993 822 783 685 191
SfoI GGC'GCC
Cuts at: 2027 2275 2027
Size: 248 4347
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4595
SmaI CCC'GGG
Cuts at: 1617 1617
Size: 4595
SmlI C'TyrA_G
Cuts at: 649 1101 1750 2665 3157 3419 649
Size: 452 649 915 492 262 1825
Fragments arranged by size:
1825 915 649 492 452 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4595
SphI G_CATG'C
Cuts at: 1648 2148 1648
Size: 500 4095
StuI AGG'CCT
Cuts at: 844 1408 844
Size: 564 4031
StyI C'CwwG_G
Cuts at: 107 443 1729 2083 107
Size: 336 1286 354 2619
Fragments arranged by size:
2619 1286 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 1477 2178 2311 2770 2979 163
Size: 1314 701 133 459 209 1779
Fragments arranged by size:
1779 1314 701 459 209 133
TatI w'GTAC_w
Cuts at: 941 4110 941
Size: 3169 1426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4563
Tth111I GACn'n_nGTC
Cuts at: 2392 2392
Size: 4595
XbaI T'CTAG_A
Cuts at: 1626 1626
Size: 4595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2090
Size: 4595
XmaI C'CCGG_G
Cuts at: 1615 1615
Size: 4595
XmnI GAAnn'nnTTC
Cuts at: 723 1387 723
Size: 664 3931
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BmgBI BpmI BsiWI BsmBI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NotI NruI PacI PflMI PmlI
PshAI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI