Restriction map for
pF1KE0029
(Circular) (Six-base) MAPSORT of: pF1KE0029.seq Check: 9235 from: 1 to: 4703
pF1KE0029 4703 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1741 1741
Size: 4703
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2960 3707 3975 2528
Size: 432 747 268 3256
Fragments arranged by size:
3256 747 432 268
AflII C'TTAA_G
Cuts at: 319 1230 1434 319
Size: 911 204 3588
Fragments arranged by size:
3588 911 204
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 635 3091 635
Size: 2456 2247
AlwNI CAG_nnn'CTG
Cuts at: 1279 2008 3575 1279
Size: 729 1567 2407
Fragments arranged by size:
2407 1567 729
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 151 428 811 1707 4056 4312 151
Size: 277 383 896 2349 256 542
Fragments arranged by size:
2349 896 542 383 277 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 1304 1723 1304
Size: 419 4284
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 203 236 203
Size: 33 4670
BamHI G'GATC_C
Cuts at: 535 804 1728 535
Size: 269 924 3510
Fragments arranged by size:
3510 924 269
BanI G'GyrC_C
Cuts at: 1416 1719 2133 2381 2416 1416
Size: 303 414 248 35 3703
Fragments arranged by size:
3703 414 303 248 35
BanII G_rGCy'C
Cuts at: 263 296 909 1303 1625 1641 1717 2747 263
Size: 33 613 394 322 16 76 1030 2219
Fragments arranged by size:
2219 1030 613 394 322 76 33 16
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 1595 1595
Size: 4703
BbvCI CC'TCA_GC
Cuts at: 1103 1177 1627 1103
Size: 74 450 4179
Fragments arranged by size:
4179 450 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4669
BciVI GTATCCnnnnn_n'
Cuts at: 2601 3362 4703 2601
Size: 761 1341 2601
Fragments arranged by size:
2601 1341 761
BclI T'GATC_A
Cuts at: 2223 2223
Size: 4703
BglII A'GATC_T
Cuts at: 2218 4255 2218
Size: 2037 2666
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 2328 2421 3477 2328
Size: 93 1056 3554
Fragments arranged by size:
3554 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1256 1915 1996 2331 4475 1256
Size: 659 81 335 2144 1484
Fragments arranged by size:
2144 1484 659 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1061 3070 1061
Size: 2009 2694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 286
Size: 4703
Bpu10I CC'TnA_GC
Cuts at: 1103 1177 1393 1627 1103
Size: 74 216 234 4179
Fragments arranged by size:
4179 234 216 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1449 1879 2758 3250 3548 1449
Size: 430 879 492 298 2604
Fragments arranged by size:
2604 879 492 430 298
BsaI GGTCTCn'nnnn_
Cuts at: 4407 4407
Size: 4703
BsaAI yAC'GTr
Cuts at: 2056 2056
Size: 4703
BsaBI GATnn'nnATC
Cuts at: 6 602 1161 2217 4254 6
Size: 596 559 1056 2037 455
Fragments arranged by size:
2037 1056 596 559 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 203 635 1445 1892 2413 3091 3365 3512 3923
Size: 432 810 447 521 678 274 147 411
Cuts at: 3923 203
Size: 983
Fragments arranged by size:
983 810 678 521 447 432 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1133 1138 1200 1615 1133
Size: 5 62 415 4221
Fragments arranged by size:
4221 415 62 5
BseYI C'CCAG_C
Cuts at: 111 908 2128 2886 3463 3959 111
Size: 797 1220 758 577 496 855
Fragments arranged by size:
1220 855 797 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 611
Size: 4703
BsiEI CG_ry'CG
Cuts at: 104 2245 2291 3499 104
Size: 2141 46 1208 1308
Fragments arranged by size:
2141 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 263 296 1625 1717 2495 2685 3477 263
Size: 33 1329 92 778 190 792 1489
Fragments arranged by size:
1489 1329 792 778 190 92 33
Bsp1286I G_dGCh'C
Cuts at: 263 296 909 1303 1625 1641 1717 2328 2421
Size: 33 613 394 322 16 76 611 93
Cuts at: 2421 2495 2685 2747 3477 263
Size: 74 190 62 730 1489
Fragments arranged by size:
1489 730 613 611 394 322 190 93
76 74 62 33 16
BspEI T'CCGG_A
Cuts at: 1445 3923 1445
Size: 2478 2225
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1753 1967 2269 2650 1753
Size: 214 302 381 3806
Fragments arranged by size:
3806 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2995 3049 4533 4698 50
Size: 2945 54 1484 165 55
Fragments arranged by size:
2945 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2615 2615
Size: 4703
BsrFI r'CCGG_y
Cuts at: 635 1592 2701 2882 3091 635
Size: 957 1109 181 209 2247
Fragments arranged by size:
2247 1109 957 209 181
BsrGI T'GTAC_A
Cuts at: 1332 1332
Size: 4703
BssSI C'ACGA_G
Cuts at: 2974 3332 2974
Size: 358 4345
BstAPI GCAn_nnn'nTGC
Cuts at: 342 2080 342
Size: 1738 2965
BstBI TT'CG_AA
Cuts at: 3064 3064
Size: 4703
BstYI r'GATC_y
Cuts at: 1 535 804 1728 2195 2218 2553 2799 3811
Size: 534 269 924 467 23 335 246 1012
Cuts at: 3811 3897 3909 4255 1
Size: 86 12 346 449
Fragments arranged by size:
1012 924 534 467 449 346 335 269
246 86 23 12
BtgI C'CryG_G
Cuts at: 539 754 539
Size: 215 4488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2653 2834 2997 2653
Size: 181 163 4359
Fragments arranged by size:
4359 181 163
BtsI GCAGTG_nn'
Cuts at: 331 623 1946 331
Size: 292 1323 3088
Fragments arranged by size:
3088 1323 292
ClaI AT'CG_AT
Cuts at: 1563 1563
Size: 4703
DraI TTT'AAA
Cuts at: 1702 1702
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2409 3267 1653
Size: 756 858 3089
Fragments arranged by size:
3089 858 756
EaeI y'GGCC_r
Cuts at: 873 1407 2288 2462 2853 2880 4225 873
Size: 534 881 174 391 27 1345 1351
Fragments arranged by size:
1351 1345 881 534 391 174 27
EagI C'GGCC_G
Cuts at: 2288 2288
Size: 4703
EarI CTCTTCn'nnn_
Cuts at: 1086 1218 2726 2936 1086
Size: 132 1508 210 2853
Fragments arranged by size:
2853 1508 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2911 3221 3367 4513 2911
Size: 310 146 1146 3101
Fragments arranged by size:
3101 1146 310 146
EcoICRI GAG'CTC
Cuts at: 261 294 1623 1715 261
Size: 33 1329 92 3249
Fragments arranged by size:
3249 1329 92 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 2528 2960 3707 3975 286
Size: 2242 432 747 268 1014
Fragments arranged by size:
2242 1014 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 874 874
Size: 4703
EcoO109I rG'GnC_Cy
Cuts at: 867 1842 867
Size: 975 3728
EcoRI G'AATT_C
Cuts at: 151 1707 151
Size: 1556 3147
EcoRV GAT'ATC
Cuts at: 894 894
Size: 4703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4703
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 2137 2385 3407 4386 2137
Size: 248 1022 979 2454
Fragments arranged by size:
2454 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2545 2575 2577 2607 2545
Size: 30 2 30 4641
Fragments arranged by size:
4641 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
HindIII A'AGCT_T
Cuts at: 1227 1227
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MfeI C'AATT_G
Cuts at: 530 1190 530
Size: 660 4043
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 700 1051 3374 3558 700
Size: 351 2323 184 1845
Fragments arranged by size:
2323 1845 351 184
MscI TGG'CCA
Cuts at: 875 1409 2464 875
Size: 534 1055 3114
Fragments arranged by size:
3114 1055 534
MslI CAynn'nnrTG
Cuts at: 2692 2819 2692
Size: 127 4576
MspA1I CmG'CkG
Cuts at: 285 1814 2128 2488 3501 3746 285
Size: 1529 314 360 1013 245 1242
Fragments arranged by size:
1529 1242 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2884 2884
Size: 4703
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 539 539
Size: 4703
NgoMIV G'CCGG_C
Cuts at: 2882 2882
Size: 4703
NspI r_CATG'y
Cuts at: 1756 2256 3163 1756
Size: 500 907 3296
Fragments arranged by size:
3296 907 500
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PfoI T'CCnGG_A
Cuts at: 263 263
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PpuMI rG'GwC_Cy
Cuts at: 867 867
Size: 4703
PsiI TTA'TAA
Cuts at: 671 671
Size: 4703
PstI C_TGCA'G
Cuts at: 338 1586 1750 2435 338
Size: 1248 164 685 2606
Fragments arranged by size:
2606 1248 685 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 285 2128 2488 285
Size: 1843 360 2500
Fragments arranged by size:
2500 1843 360
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 263 296 1625 1717 263
Size: 33 1329 92 3249
Fragments arranged by size:
3249 1329 92 33
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 2726 2936 2726
Size: 210 4493
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SfcI C'TryA_G
Cuts at: 33 334 1582 1746 2431 3424 3615 33
Size: 301 1248 164 685 993 191 1121
Fragments arranged by size:
1248 1121 993 685 301 191 164
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1725 1725
Size: 4703
SmlI C'TyrA_G
Cuts at: 319 1230 1304 1434 1464 1858 2773 3265 3527
Size: 911 74 130 30 394 915 492 262
Cuts at: 3527 319
Size: 1495
Fragments arranged by size:
1495 915 911 492 394 262 130 74
30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4703
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
StyI C'CwwG_G
Cuts at: 539 870 1259 1837 2191 539
Size: 331 389 578 354 3051
Fragments arranged by size:
3051 578 389 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2286 2419 2878 3087 2286
Size: 133 459 209 3902
Fragments arranged by size:
3902 459 209 133
TatI w'GTAC_w
Cuts at: 1332 4218 1332
Size: 2886 1817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1440 1472 4220 4252 1440
Size: 32 2748 32 1891
Fragments arranged by size:
2748 1891 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 353 2198 353
Size: 1845 2858
XhoI C'TCGA_G
Cuts at: 1304 1304
Size: 4703
XmaI C'CCGG_G
Cuts at: 1723 1723
Size: 4703
XmnI GAAnn'nnTTC
Cuts at: 416 416
Size: 4703
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I CspCI
DraIII FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI