Restriction map for
pF1KE0026
(Circular) (Six-base) MAPSORT of: pF1KE0026.seq Check: 9765 from: 1 to: 4568
pF1KE0026 4568 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1244 1262 1606 1244
Size: 18 344 4206
Fragments arranged by size:
4206 344 18
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 442 583 1021 2393 2825 3572 3840 406
Size: 36 141 438 1372 432 747 268 1134
Fragments arranged by size:
1372 1134 747 438 432 268 141 36
AflIII A'CryG_T
Cuts at: 1077 1920 3024 4001 1077
Size: 843 1104 977 1644
Fragments arranged by size:
1644 1104 977 843
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 212 786 828 1039 1107 1873 3440 212
Size: 574 42 211 68 766 1567 1340
Fragments arranged by size:
1567 1340 766 574 211 68 42
ApaLI G'TGCA_C
Cuts at: 1081 3338 1081
Size: 2257 2311
ApoI r'AATT_y
Cuts at: 1572 3921 4177 1572
Size: 2349 256 1963
Fragments arranged by size:
2349 1963 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 1588 1588
Size: 4568
BamHI G'GATC_C
Cuts at: 111 1029 1593 111
Size: 918 564 3086
Fragments arranged by size:
3086 918 564
BanI G'GyrC_C
Cuts at: 1327 1475 1584 1998 2246 2281 1327
Size: 148 109 414 248 35 3614
Fragments arranged by size:
3614 414 248 148 109 35
BanII G_rGCy'C
Cuts at: 185 294 809 1582 2612 185
Size: 109 515 773 1030 2141
Fragments arranged by size:
2141 1030 773 515 109
BbeI G_GCGC'C
Cuts at: 1479 2002 2250 1479
Size: 523 248 3797
Fragments arranged by size:
3797 523 248
BbsI GAAGACnn'nnnn_
Cuts at: 970 1009 970
Size: 39 4529
BbvCI CC'TCA_GC
Cuts at: 1300 1300
Size: 4568
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglI GCCn_nnn'nGGC
Cuts at: 324 324
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4568
Bme1580I G_kGCm'C
Cuts at: 1085 2193 2286 3342 1085
Size: 1108 93 1056 2311
Fragments arranged by size:
2311 1108 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 273 991 1194 1780 1861 2196 4340 273
Size: 718 203 586 81 335 2144 501
Fragments arranged by size:
2144 718 586 501 335 203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 808 976 808
Size: 168 4400
Bpu10I CC'TnA_GC
Cuts at: 361 1300 361
Size: 939 3629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1744 2623 3115 3413 1744
Size: 879 492 298 2899
Fragments arranged by size:
2899 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 766 4272 766
Size: 3506 1062
BsaAI yAC'GTr
Cuts at: 1921 1921
Size: 4568
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1476 1999 2247 2949 4445 1476
Size: 523 248 702 1496 1599
Fragments arranged by size:
1599 1496 702 523 248
BsaWI w'CCGG_w
Cuts at: 1247 1757 2278 2956 3230 3377 3788 1247
Size: 510 521 678 274 147 411 2027
Fragments arranged by size:
2027 678 521 510 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 517 547 1420 1450 517
Size: 30 873 30 3635
Fragments arranged by size:
3635 873 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1158 1355 1520 1158
Size: 197 165 4206
Fragments arranged by size:
4206 197 165
BseYI C'CCAG_C
Cuts at: 720 1993 2751 3328 3824 720
Size: 1273 758 577 496 1464
Fragments arranged by size:
1464 1273 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3364 104
Size: 2006 46 1208 1308
Fragments arranged by size:
2006 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 294 809 1085 1582 2360 2550 3342 294
Size: 515 276 497 778 190 792 1520
Fragments arranged by size:
1520 792 778 515 497 276 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1057 1057
Size: 4568
Bsp1286I G_dGCh'C
Cuts at: 185 294 809 1085 1582 2193 2286 2360 2550
Size: 109 515 276 497 611 93 74 190
Cuts at: 2550 2612 3342 185
Size: 62 730 1411
Fragments arranged by size:
1411 730 611 515 497 276 190 109
93 74 62
BspEI T'CCGG_A
Cuts at: 3788 3788
Size: 4568
BspHI T'CATG_A
Cuts at: 543 3744 543
Size: 3201 1367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 1618 1832 2134 2515 320
Size: 1298 214 302 381 2373
Fragments arranged by size:
2373 1298 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2860 2914 4398 4563 50
Size: 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 1175 1356 2566 2747 2956 1175
Size: 181 1210 181 209 2787
Fragments arranged by size:
2787 1210 209 181 181
BssSI C'ACGA_G
Cuts at: 2839 3197 2839
Size: 358 4210
BstAPI GCAn_nnn'nTGC
Cuts at: 1945 1945
Size: 4568
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstEII G'GTnAC_C
Cuts at: 391 391
Size: 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 123 123
Size: 4568
BstYI r'GATC_y
Cuts at: 1 111 242 995 1029 1593 2060 2083 2418
Size: 110 131 753 34 564 467 23 335
Cuts at: 2418 2664 3676 3762 3774 4120 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 753 564 467 449 346 335 246
131 110 86 34 23 12
Bsu36I CC'TnA_GG
Cuts at: 175 1170 175
Size: 995 3573
BtgI C'CryG_G
Cuts at: 107 408 107
Size: 301 4267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 1523 1811 1523
Size: 288 4280
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 201 332 746 1535 2153 2327 2718 2745 4090
Size: 131 414 789 618 174 391 27 1345
Cuts at: 4090 201
Size: 679
Fragments arranged by size:
1345 789 679 618 414 391 174 131
27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 572 2591 2801 572
Size: 2019 210 2339
Fragments arranged by size:
2339 2019 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2776 3086 3232 4378 2776
Size: 310 146 1146 2966
Fragments arranged by size:
2966 1146 310 146
EcoICRI GAG'CTC
Cuts at: 292 807 1580 292
Size: 515 773 3280
Fragments arranged by size:
3280 773 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 442 583 808 976 1021 2393 2825 3572
Size: 36 141 225 168 45 1372 432 747
Cuts at: 3572 3840 406
Size: 268 1134
Fragments arranged by size:
1372 1134 747 432 268 225 168 141
45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 174 174
Size: 4568
EcoO109I rG'GnC_Cy
Cuts at: 549 825 1218 1707 549
Size: 276 393 489 3410
Fragments arranged by size:
3410 489 393 276
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2587 2587
Size: 4568
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 408 1479 2002 2250 3272 4251 408
Size: 1071 523 248 1022 979 725
Fragments arranged by size:
1071 1022 979 725 523 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 281 313 1420 1452 2410 2440 2442 2472 281
Size: 32 1107 32 958 30 2 30 2377
Fragments arranged by size:
2377 1107 958 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4568
HindIII A'AGCT_T
Cuts at: 589 589
Size: 4568
KasI G'GCGC_C
Cuts at: 1475 1998 2246 1475
Size: 523 248 3797
Fragments arranged by size:
3797 523 248
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3239 3423 3239
Size: 184 4384
MscI TGG'CCA
Cuts at: 203 334 748 1537 2329 203
Size: 131 414 789 792 2442
Fragments arranged by size:
2442 792 789 414 131
MslI CAynn'nnrTG
Cuts at: 163 2557 2684 163
Size: 2394 127 2047
Fragments arranged by size:
2394 2047 127
MspA1I CmG'CkG
Cuts at: 221 330 720 759 769 878 1015 1679 1993
Size: 109 390 39 10 109 137 664 314
Cuts at: 1993 2353 3366 3611 221
Size: 360 1013 245 1178
Fragments arranged by size:
1178 1013 664 390 360 314 245 137
109 109 39 10
NaeI GCC'GGC
Cuts at: 1177 2749 1177
Size: 1572 2996
NarI GG'CG_CC
Cuts at: 1476 1999 2247 1476
Size: 523 248 3797
Fragments arranged by size:
3797 523 248
NcoI C'CATG_G
Cuts at: 107 408 107
Size: 301 4267
NgoMIV G'CCGG_C
Cuts at: 1175 2747 1175
Size: 1572 2996
NspI r_CATG'y
Cuts at: 1621 2121 3028 1621
Size: 500 907 3161
Fragments arranged by size:
3161 907 500
PasI CC'CwG_GG
Cuts at: 1471 1471
Size: 4568
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PfoI T'CCnGG_A
Cuts at: 372 372
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 825 825
Size: 4568
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 253 285 253
Size: 32 4536
PstI C_TGCA'G
Cuts at: 469 1615 2300 469
Size: 1146 685 2737
Fragments arranged by size:
2737 1146 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 221 330 720 1015 1993 2353 221
Size: 109 390 295 978 360 2436
Fragments arranged by size:
2436 978 390 360 295 109
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 294 809 1582 294
Size: 515 773 3280
Fragments arranged by size:
3280 773 515
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 465 754 1503 1611 2296 3289 3480 33
Size: 432 289 749 108 685 993 191 1121
Fragments arranged by size:
1121 993 749 685 432 289 191 108
SfoI GGC'GCC
Cuts at: 1477 2000 2248 1477
Size: 523 248 3797
Fragments arranged by size:
3797 523 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 1723 2638 3130 3392 1723
Size: 915 492 262 2899
Fragments arranged by size:
2899 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StuI AGG'CCT
Cuts at: 463 583 870 463
Size: 120 287 4161
Fragments arranged by size:
4161 287 120
StyI C'CwwG_G
Cuts at: 107 122 304 408 1702 2056 107
Size: 15 182 104 1294 354 2619
Fragments arranged by size:
2619 1294 354 182 104 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 2151 2284 2743 2952 1278
Size: 873 133 459 209 2894
Fragments arranged by size:
2894 873 459 209 133
TatI w'GTAC_w
Cuts at: 527 978 4083 527
Size: 451 3105 1012
Fragments arranged by size:
3105 1012 451
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 517 549 1274 1306 1370 1402 4085 4117 517
Size: 32 725 32 64 32 2683 32 968
Fragments arranged by size:
2683 968 725 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 783 1545 2063 123
Size: 660 762 518 2628
Fragments arranged by size:
2628 762 660 518
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
XmnI GAAnn'nnTTC
Cuts at: 427 427
Size: 4568
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI