Restriction map for
pF1KE0023
(Circular) (Six-base) MAPSORT of: pF1KE0023.seq Check: 1436 from: 1 to: 5978
pF1KE0023 5978 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1103 3016 1103
Size: 1913 4065
Acc65I G'GTAC_C
Cuts at: 2994 2994
Size: 5978
AclI AA'CG_TT
Cuts at: 1071 5818 1071
Size: 4747 1231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1796 2090 2647 3803 4235 4982 5250 809
Size: 987 294 557 1156 432 747 268 1537
Fragments arranged by size:
1537 1156 987 747 557 432 294 268
AfeI AGC'GCT
Cuts at: 1463 1463
Size: 5978
AflIII A'CryG_T
Cuts at: 239 2112 3330 4434 5411 239
Size: 1873 1218 1104 977 806
Fragments arranged by size:
1873 1218 1104 977 806
AgeI A'CCGG_T
Cuts at: 4366 4366
Size: 5978
AlwNI CAG_nnn'CTG
Cuts at: 3283 4850 3283
Size: 1567 4411
ApaLI G'TGCA_C
Cuts at: 4748 4748
Size: 5978
ApoI r'AATT_y
Cuts at: 331 370 543 641 797 1132 1252 1609 1623
Size: 39 173 98 156 335 120 357 14
Cuts at: 1623 2005 2370 2670 2982 5331 5587 331
Size: 382 365 300 312 2349 256 722
Fragments arranged by size:
2349 722 382 365 357 335 312 300
256 173 156 120 98 39 14
AseI AT'TA_AT
Cuts at: 20 929 20
Size: 909 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
AvaI C'yCGr_G
Cuts at: 2998 2998
Size: 5978
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2024 2057 2024
Size: 33 5945
BamHI G'GATC_C
Cuts at: 3003 3003
Size: 5978
BanI G'GyrC_C
Cuts at: 2994 3408 3656 3691 2994
Size: 414 248 35 5281
Fragments arranged by size:
5281 414 248 35
BanII G_rGCy'C
Cuts at: 115 1891 2710 2992 4022 115
Size: 1776 819 282 1030 2071
Fragments arranged by size:
2071 1776 1030 819 282
BbeI G_GCGC'C
Cuts at: 3412 3660 3412
Size: 248 5730
BbsI GAAGACnn'nnnn_
Cuts at: 2924 2924
Size: 5978
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 365 399 2787 2821 365
Size: 34 2388 34 3522
Fragments arranged by size:
3522 2388 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1394 3876 4637 5978 1394
Size: 2482 761 1341 1394
Fragments arranged by size:
2482 1394 1341 761
BclI T'GATC_A
Cuts at: 1083 3498 1083
Size: 2415 3563
BfrBI ATG'CAT
Cuts at: 602 2529 602
Size: 1927 4051
BglII A'GATC_T
Cuts at: 721 1305 3493 5530 721
Size: 584 2188 2037 1169
Fragments arranged by size:
2188 2037 1169 584
BlpI GC'TnA_GC
Cuts at: 3090 3090
Size: 5978
Bme1580I G_kGCm'C
Cuts at: 3603 3696 4752 3603
Size: 93 1056 4829
Fragments arranged by size:
4829 1056 93
BmgBI CAC'GTC
Cuts at: 2801 2801
Size: 5978
BmrI ACTGGGnnnn_n'
Cuts at: 3190 3271 3606 5750 3190
Size: 81 335 2144 3418
Fragments arranged by size:
3418 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2265 2729 4345 2265
Size: 464 1616 3898
Fragments arranged by size:
3898 1616 464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1844 2530 2561 2834 1844
Size: 686 31 273 4988
Fragments arranged by size:
4988 686 273 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 738 3154 4033 4525 4823 419
Size: 319 2416 879 492 298 1574
Fragments arranged by size:
2416 1574 879 492 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 1120 2828 5682 1120
Size: 1708 2854 1416
Fragments arranged by size:
2854 1708 1416
BsaAI yAC'GTr
Cuts at: 625 3331 625
Size: 2706 3272
BsaBI GATnn'nnATC
Cuts at: 6 3492 5529 6
Size: 3486 2037 455
Fragments arranged by size:
3486 2037 455
BsaHI Gr'CG_yC
Cuts at: 3409 3657 4359 5855 3409
Size: 248 702 1496 3532
Fragments arranged by size:
3532 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3167 3688 4366 4640 4787 5198 3167
Size: 521 678 274 147 411 3947
Fragments arranged by size:
3947 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1454 2311 1454
Size: 857 5121
BseYI C'CCAG_C
Cuts at: 3403 4161 4738 5234 3403
Size: 758 577 496 4147
Fragments arranged by size:
4147 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3520 3566 4774 104
Size: 3416 46 1208 1308
Fragments arranged by size:
3416 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 1891 2992 3770 3960 4752 115
Size: 1776 1101 778 190 792 1341
Fragments arranged by size:
1776 1341 1101 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 115 1891 2710 2992 3603 3696 3770 3960 4022
Size: 1776 819 282 611 93 74 190 62
Cuts at: 4022 4752 115
Size: 730 1341
Fragments arranged by size:
1776 1341 819 730 611 282 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5198 5198
Size: 5978
BspHI T'CATG_A
Cuts at: 5154 5154
Size: 5978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 696 3028 3242 3544 3925 696
Size: 2332 214 302 381 2749
Fragments arranged by size:
2749 2332 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4270 4324 5808 5973 50
Size: 4220 54 1484 165 55
Fragments arranged by size:
4220 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 194 381 521 602 3890 194
Size: 187 140 81 3288 2282
Fragments arranged by size:
3288 2282 187 140 81
BsrFI r'CCGG_y
Cuts at: 3976 4157 4366 3976
Size: 181 209 5588
Fragments arranged by size:
5588 209 181
BsrGI T'GTAC_A
Cuts at: 2724 2724
Size: 5978
BssSI C'ACGA_G
Cuts at: 4249 4607 4249
Size: 358 5620
BstAPI GCAn_nnn'nTGC
Cuts at: 196 3355 196
Size: 3159 2819
BstBI TT'CG_AA
Cuts at: 4339 4339
Size: 5978
BstEII G'GTnAC_C
Cuts at: 123 123
Size: 5978
BstXI CCAn_nnnn'nTGG
Cuts at: 1429 1429
Size: 5978
BstYI r'GATC_y
Cuts at: 1 721 1155 1305 3003 3470 3493 3828 4074
Size: 720 434 150 1698 467 23 335 246
Cuts at: 4074 5086 5172 5184 5530 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1698 1012 720 467 449 434 346 335
246 150 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3928 4109 4272 3928
Size: 181 163 5634
Fragments arranged by size:
5634 181 163
BtsI GCAGTG_nn'
Cuts at: 2295 3221 2295
Size: 926 5052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 572 607 572
Size: 35 5943
DraI TTT'AAA
Cuts at: 2412 2505 2909 2977 2412
Size: 93 404 68 5413
Fragments arranged by size:
5413 404 93 68
DrdI GACnn_nn'nnGTC
Cuts at: 3684 4542 3684
Size: 858 5120
EaeI y'GGCC_r
Cuts at: 2052 3563 3737 4128 4155 5500 2052
Size: 1511 174 391 27 1345 2530
Fragments arranged by size:
2530 1511 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3563 3563
Size: 5978
EarI CTCTTCn'nnn_
Cuts at: 1727 2066 2288 2546 2609 4001 4211 1727
Size: 339 222 258 63 1392 210 3494
Fragments arranged by size:
3494 1392 339 258 222 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4186 4496 4642 5788 4186
Size: 310 146 1146 4376
Fragments arranged by size:
4376 1146 310 146
EcoICRI GAG'CTC
Cuts at: 113 1889 2990 113
Size: 1776 1101 3101
Fragments arranged by size:
3101 1776 1101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 1796 1844 2090 2530 2561 2647 2834 3803
Size: 987 48 246 440 31 86 187 969
Cuts at: 3803 4235 4982 5250 809
Size: 432 747 268 1537
Fragments arranged by size:
1537 987 969 747 440 432 268 246
187 86 48 31
EcoO109I rG'GnC_Cy
Cuts at: 3117 3117
Size: 5978
EcoRI G'AATT_C
Cuts at: 370 2370 2670 2982 370
Size: 2000 300 312 3366
Fragments arranged by size:
3366 2000 312 300
EcoRV GAT'ATC
Cuts at: 2384 2384
Size: 5978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1273 3997 1273
Size: 2724 3254
FspI TGC'GCA
Cuts at: 3759 3759
Size: 5978
HaeII r_GCGC'y
Cuts at: 169 1465 3412 3660 4682 5661 169
Size: 1296 1947 248 1022 979 486
Fragments arranged by size:
1947 1296 1022 979 486 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1330 1362 1551 1583 3820 3850 3852 3882 1330
Size: 32 189 32 2237 30 2 30 3426
Fragments arranged by size:
3426 2237 189 32 32 30 30 2
HincII GTy'rAC
Cuts at: 894 3017 894
Size: 2123 3855
HindIII A'AGCT_T
Cuts at: 2218 2218
Size: 5978
HpaI GTT'AAC
Cuts at: 894 894
Size: 5978
KasI G'GCGC_C
Cuts at: 3408 3656 3408
Size: 248 5730
KpnI G_GTAC'C
Cuts at: 2998 2998
Size: 5978
MluI A'CGCG_T
Cuts at: 5411 5411
Size: 5978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1270 4649 4833 1270
Size: 3379 184 2415
Fragments arranged by size:
3379 2415 184
MscI TGG'CCA
Cuts at: 2054 3739 2054
Size: 1685 4293
MslI CAynn'nnrTG
Cuts at: 386 1558 2132 3967 4094 386
Size: 1172 574 1835 127 2270
Fragments arranged by size:
2270 1835 1172 574 127
MspA1I CmG'CkG
Cuts at: 3089 3403 3763 4776 5021 3089
Size: 314 360 1013 245 4046
Fragments arranged by size:
4046 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4159 4159
Size: 5978
NarI GG'CG_CC
Cuts at: 3409 3657 3409
Size: 248 5730
NdeI CA'TA_TG
Cuts at: 1166 1166
Size: 5978
NgoMIV G'CCGG_C
Cuts at: 4157 4157
Size: 5978
NsiI A_TGCA'T
Cuts at: 604 2531 604
Size: 1927 4051
NspI r_CATG'y
Cuts at: 243 2116 2885 3031 3531 4438 243
Size: 1873 769 146 500 907 1783
Fragments arranged by size:
1873 1783 907 769 500 146
PciI A'CATG_T
Cuts at: 239 2112 4434 239
Size: 1873 2322 1783
Fragments arranged by size:
2322 1873 1783
PfoI T'CCnGG_A
Cuts at: 2447 2447
Size: 5978
PmeI GTTT'AAAC
Cuts at: 2977 2977
Size: 5978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5117 5149 5117
Size: 32 5946
PstI C_TGCA'G
Cuts at: 164 3025 3710 164
Size: 2861 685 2432
Fragments arranged by size:
2861 2432 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5978
PvuII CAG'CTG
Cuts at: 3403 3763 3403
Size: 360 5618
RsrII CG'GwC_CG
Cuts at: 4173 4173
Size: 5978
SacI G_AGCT'C
Cuts at: 115 1891 2992 115
Size: 1776 1101 3101
Fragments arranged by size:
3101 1776 1101
SalI G'TCGA_C
Cuts at: 3015 3015
Size: 5978
SapI GCTCTTCn'nnn_
Cuts at: 2609 4001 4211 2609
Size: 1392 210 4376
Fragments arranged by size:
4376 1392 210
SbfI CC_TGCA'GG
Cuts at: 3025 3025
Size: 5978
ScaI AGT'ACT
Cuts at: 1541 5495 1541
Size: 3954 2024
SfcI C'TryA_G
Cuts at: 33 160 1301 3021 3706 4699 4890 33
Size: 127 1141 1720 685 993 191 1121
Fragments arranged by size:
1720 1141 1121 993 685 191 127
SfoI GGC'GCC
Cuts at: 3410 3658 3410
Size: 248 5730
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5978
SmaI CCC'GGG
Cuts at: 3000 3000
Size: 5978
SmlI C'TyrA_G
Cuts at: 434 717 3133 4048 4540 4802 434
Size: 283 2416 915 492 262 1610
Fragments arranged by size:
2416 1610 915 492 283 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5978
SphI G_CATG'C
Cuts at: 3031 3531 3031
Size: 500 5478
SspI AAT'ATT
Cuts at: 933 2312 933
Size: 1379 4599
StyI C'CwwG_G
Cuts at: 937 1454 3112 3466 937
Size: 517 1658 354 3449
Fragments arranged by size:
3449 1658 517 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 111 800 867 2272 3561 3694 4153 4362 111
Size: 689 67 1405 1289 133 459 209 1727
Fragments arranged by size:
1727 1405 1289 689 459 209 133 67
TatI w'GTAC_w
Cuts at: 1539 2724 5493 1539
Size: 1185 2769 2024
Fragments arranged by size:
2769 2024 1185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 197 229 396 428 5495 5527 197
Size: 32 167 32 5067 32 648
Fragments arranged by size:
5067 648 167 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3775 3775
Size: 5978
XbaI T'CTAG_A
Cuts at: 2544 3009 2544
Size: 465 5513
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2505 3473 2505
Size: 968 5010
XmaI C'CCGG_G
Cuts at: 2998 2998
Size: 5978
XmnI GAAnn'nnTTC
Cuts at: 797 1937 2397 797
Size: 1140 460 4378
Fragments arranged by size:
4378 1140 460
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BglI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I BtgI ClaI
DraIII EcoNI FseI FspAI MfeI NcoI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI