Restriction map for
pF1KE0019
(Circular) (Six-base) MAPSORT of: pF1KE0019.seq Check: 6055 from: 1 to: 4889
pF1KE0019 4889 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1927 1927
Size: 4889
Acc65I G'GTAC_C
Cuts at: 1905 1905
Size: 4889
AclI AA'CG_TT
Cuts at: 4729 4729
Size: 4889
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 2714 3146 3893 4161 1043
Size: 1671 432 747 268 1771
Fragments arranged by size:
1771 1671 747 432 268
AflIII A'CryG_T
Cuts at: 2241 3345 4322 2241
Size: 1104 977 2808
Fragments arranged by size:
2808 1104 977
AgeI A'CCGG_T
Cuts at: 3277 3277
Size: 4889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1147 1147
Size: 4889
AlwNI CAG_nnn'CTG
Cuts at: 2194 3761 2194
Size: 1567 3322
ApaI G_GGCC'C
Cuts at: 384 384
Size: 4889
ApaLI G'TGCA_C
Cuts at: 1736 3659 1736
Size: 1923 2966
ApoI r'AATT_y
Cuts at: 1033 1893 4242 4498 1033
Size: 860 2349 256 1424
Fragments arranged by size:
2349 1424 860 256
AseI AT'TA_AT
Cuts at: 20 1211 20
Size: 1191 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
AvaI C'yCGr_G
Cuts at: 1909 1909
Size: 4889
BamHI G'GATC_C
Cuts at: 1914 1914
Size: 4889
BanI G'GyrC_C
Cuts at: 1905 2319 2567 2602 1905
Size: 414 248 35 4192
Fragments arranged by size:
4192 414 248 35
BanII G_rGCy'C
Cuts at: 384 1472 1903 2933 384
Size: 1088 431 1030 2340
Fragments arranged by size:
2340 1088 1030 431
BbeI G_GCGC'C
Cuts at: 2323 2571 2323
Size: 248 4641
BbsI GAAGACnn'nnnn_
Cuts at: 1370 1370
Size: 4889
BbvCI CC'TCA_GC
Cuts at: 1116 1116
Size: 4889
BciVI GTATCCnnnnn_n'
Cuts at: 2787 3548 4889 2787
Size: 761 1341 2787
Fragments arranged by size:
2787 1341 761
BclI T'GATC_A
Cuts at: 1805 2409 1805
Size: 604 4285
BglII A'GATC_T
Cuts at: 2404 4441 2404
Size: 2037 2852
BlpI GC'TnA_GC
Cuts at: 2001 2001
Size: 4889
Bme1580I G_kGCm'C
Cuts at: 384 1740 2514 2607 3663 384
Size: 1356 774 93 1056 1610
Fragments arranged by size:
1610 1356 1056 774 93
BmrI ACTGGGnnnn_n'
Cuts at: 336 1839 2101 2182 2517 4661 336
Size: 1503 262 81 335 2144 564
Fragments arranged by size:
2144 1503 564 335 262 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1179 3256 1179
Size: 2077 2812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1691 679
Size: 1012 3877
Bpu10I CC'TnA_GC
Cuts at: 1116 1116
Size: 4889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 2065 2944 3436 3734 244
Size: 1821 879 492 298 1399
Fragments arranged by size:
1821 1399 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 495 1003 4593 495
Size: 508 3590 791
Fragments arranged by size:
3590 791 508
BsaAI yAC'GTr
Cuts at: 2242 2242
Size: 4889
BsaBI GATnn'nnATC
Cuts at: 6 2403 4440 6
Size: 2397 2037 455
Fragments arranged by size:
2397 2037 455
BsaHI Gr'CG_yC
Cuts at: 2320 2568 3270 4766 2320
Size: 248 702 1496 2443
Fragments arranged by size:
2443 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2078 2599 3277 3551 3698 4109 2078
Size: 521 678 274 147 411 2858
Fragments arranged by size:
2858 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1040 1043 1040
Size: 3 4886
BseYI C'CCAG_C
Cuts at: 743 2314 3072 3649 4145 743
Size: 1571 758 577 496 1487
Fragments arranged by size:
1571 1487 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1424 1741 1165
Size: 259 317 4313
Fragments arranged by size:
4313 317 259
BsiEI CG_ry'CG
Cuts at: 104 2431 2477 3685 104
Size: 2327 46 1208 1308
Fragments arranged by size:
2327 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1160 1740 1903 2681 2871 3663 1160
Size: 580 163 778 190 792 2386
Fragments arranged by size:
2386 792 778 580 190 163
Bsp1286I G_dGCh'C
Cuts at: 384 1160 1472 1740 1903 2514 2607 2681 2871
Size: 776 312 268 163 611 93 74 190
Cuts at: 2871 2933 3663 384
Size: 62 730 1610
Fragments arranged by size:
1610 776 730 611 312 268 190 163
93 74 62
BspEI T'CCGG_A
Cuts at: 4109 4109
Size: 4889
BspHI T'CATG_A
Cuts at: 4065 4065
Size: 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1118 1763 1939 2153 2455 2836 1118
Size: 645 176 214 302 381 3171
Fragments arranged by size:
3171 645 381 302 214 176
BsrBI CCG'CTC
Cuts at: 50 3181 3235 4719 4884 50
Size: 3131 54 1484 165 55
Fragments arranged by size:
3131 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2801 2801
Size: 4889
BsrFI r'CCGG_y
Cuts at: 2887 3068 3277 2887
Size: 181 209 4499
Fragments arranged by size:
4499 209 181
BsrGI T'GTAC_A
Cuts at: 444 444
Size: 4889
BssSI C'ACGA_G
Cuts at: 3160 3518 3160
Size: 358 4531
BstAPI GCAn_nnn'nTGC
Cuts at: 1514 2266 1514
Size: 752 4137
BstBI TT'CG_AA
Cuts at: 3250 3250
Size: 4889
BstYI r'GATC_y
Cuts at: 1 801 1914 2381 2404 2739 2985 3997 4083
Size: 800 1113 467 23 335 246 1012 86
Cuts at: 4083 4095 4441 1
Size: 12 346 449
Fragments arranged by size:
1113 1012 800 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1776 1776
Size: 4889
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2839 3020 3183 2839
Size: 181 163 4545
Fragments arranged by size:
4545 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 2132 1412
Size: 720 4169
DraI TTT'AAA
Cuts at: 605 1888 605
Size: 1283 3606
DraIII CAC_nnn'GTG
Cuts at: 1735 1735
Size: 4889
DrdI GACnn_nn'nnGTC
Cuts at: 2595 3453 2595
Size: 858 4031
EaeI y'GGCC_r
Cuts at: 429 1487 2474 2648 3039 3066 4411 429
Size: 1058 987 174 391 27 1345 907
Fragments arranged by size:
1345 1058 987 907 391 174 27
EagI C'GGCC_G
Cuts at: 2474 2474
Size: 4889
EarI CTCTTCn'nnn_
Cuts at: 1148 2912 3122 1148
Size: 1764 210 2915
Fragments arranged by size:
2915 1764 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3097 3407 3553 4699 3097
Size: 310 146 1146 3287
Fragments arranged by size:
3287 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1901 1901
Size: 4889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1043 1691 2714 3146 3893 4161 679
Size: 364 648 1023 432 747 268 1407
Fragments arranged by size:
1407 1023 747 648 432 364 268
EcoO109I rG'GnC_Cy
Cuts at: 380 2028 380
Size: 1648 3241
EcoRI G'AATT_C
Cuts at: 1893 1893
Size: 4889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4889
FspI TGC'GCA
Cuts at: 2670 2670
Size: 4889
HaeII r_GCGC'y
Cuts at: 2323 2571 3593 4572 2323
Size: 248 1022 979 2640
Fragments arranged by size:
2640 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 456 488 1099 1131 2731 2761 2763 2793 456
Size: 32 611 32 1600 30 2 30 2552
Fragments arranged by size:
2552 1600 611 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1928 1928
Size: 4889
HindIII A'AGCT_T
Cuts at: 1617 1617
Size: 4889
KasI G'GCGC_C
Cuts at: 2319 2567 2319
Size: 248 4641
KpnI G_GTAC'C
Cuts at: 1909 1909
Size: 4889
MluI A'CGCG_T
Cuts at: 4322 4322
Size: 4889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 154 333 1507 1655 3560 3744 154
Size: 179 1174 148 1905 184 1299
Fragments arranged by size:
1905 1299 1174 184 179 148
MscI TGG'CCA
Cuts at: 431 1489 2650 431
Size: 1058 1161 2670
Fragments arranged by size:
2670 1161 1058
MslI CAynn'nnrTG
Cuts at: 293 1798 2878 3005 293
Size: 1505 1080 127 2177
Fragments arranged by size:
2177 1505 1080 127
MspA1I CmG'CkG
Cuts at: 442 1120 2000 2314 2674 3687 3932 442
Size: 678 880 314 360 1013 245 1399
Fragments arranged by size:
1399 1013 880 678 360 314 245
NaeI GCC'GGC
Cuts at: 3070 3070
Size: 4889
NarI GG'CG_CC
Cuts at: 2320 2568 2320
Size: 248 4641
NdeI CA'TA_TG
Cuts at: 1331 1331
Size: 4889
NgoMIV G'CCGG_C
Cuts at: 3068 3068
Size: 4889
NspI r_CATG'y
Cuts at: 1134 1702 1942 2442 3349 1134
Size: 568 240 500 907 2674
Fragments arranged by size:
2674 907 568 500 240
PciI A'CATG_T
Cuts at: 3345 3345
Size: 4889
PmeI GTTT'AAAC
Cuts at: 1888 1888
Size: 4889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 4028
Size: 32 4857
PspOMI G'GGCC_C
Cuts at: 380 380
Size: 4889
PstI C_TGCA'G
Cuts at: 1663 1936 2621 1663
Size: 273 685 3931
Fragments arranged by size:
3931 685 273
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4889
PvuII CAG'CTG
Cuts at: 442 1120 2314 2674 442
Size: 678 1194 360 2657
Fragments arranged by size:
2657 1194 678 360
RsrII CG'GwC_CG
Cuts at: 3084 3084
Size: 4889
SacI G_AGCT'C
Cuts at: 1903 1903
Size: 4889
SalI G'TCGA_C
Cuts at: 1926 1926
Size: 4889
SapI GCTCTTCn'nnn_
Cuts at: 1148 2912 3122 1148
Size: 1764 210 2915
Fragments arranged by size:
2915 1764 210
SbfI CC_TGCA'GG
Cuts at: 1936 1936
Size: 4889
ScaI AGT'ACT
Cuts at: 4406 4406
Size: 4889
SfcI C'TryA_G
Cuts at: 33 1288 1659 1932 2617 3610 3801 33
Size: 1255 371 273 685 993 191 1121
Fragments arranged by size:
1255 1121 993 685 371 273 191
SfoI GGC'GCC
Cuts at: 2321 2569 2321
Size: 248 4641
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
SmaI CCC'GGG
Cuts at: 1911 1911
Size: 4889
SmlI C'TyrA_G
Cuts at: 223 2044 2959 3451 3713 223
Size: 1821 915 492 262 1399
Fragments arranged by size:
1821 1399 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4889
SphI G_CATG'C
Cuts at: 1134 1942 2442 1134
Size: 808 500 3581
Fragments arranged by size:
3581 808 500
SspI AAT'ATT
Cuts at: 273 1600 273
Size: 1327 3562
StuI AGG'CCT
Cuts at: 1141 1141
Size: 4889
StyI C'CwwG_G
Cuts at: 2023 2377 2023
Size: 354 4535
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2472 2605 3064 3273 2472
Size: 133 459 209 4088
Fragments arranged by size:
4088 459 209 133
TatI w'GTAC_w
Cuts at: 444 1354 4404 444
Size: 910 3050 929
Fragments arranged by size:
3050 929 910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4406 4438 4406
Size: 32 4857
Tth111I GACn'n_nGTC
Cuts at: 2686 2686
Size: 4889
XbaI T'CTAG_A
Cuts at: 1047 1540 1920 1047
Size: 493 380 4016
Fragments arranged by size:
4016 493 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 349 751 1197 2384 349
Size: 402 446 1187 2854
Fragments arranged by size:
2854 1187 446 402
XmaI C'CCGG_G
Cuts at: 1909 1909
Size: 4889
XmnI GAAnn'nnTTC
Cuts at: 305 305
Size: 4889
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI