Restriction map for
pF1KE0015
(Circular) (Six-base) MAPSORT of: pF1KE0015.seq Check: 1711 from: 1 to: 5066
pF1KE0015 5066 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 2104 2104
Size: 5066
Acc65I G'GTAC_C
Cuts at: 2082 2082
Size: 5066
AclI AA'CG_TT
Cuts at: 2060 4906 2060
Size: 2846 2220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 545 711 724 896 1184 1229 1241 2891
Size: 165 166 13 172 288 45 12 1650
Cuts at: 2891 3323 4070 4338 380
Size: 432 747 268 1108
Fragments arranged by size:
1650 1108 747 432 288 268 172 166
165 45 13 12
AflIII A'CryG_T
Cuts at: 2418 3522 4499 2418
Size: 1104 977 2985
Fragments arranged by size:
2985 1104 977
AgeI A'CCGG_T
Cuts at: 3454 3454
Size: 5066
AhdI GACnn_n'nnGTC
Cuts at: 131 131
Size: 5066
AlwNI CAG_nnn'CTG
Cuts at: 1207 1651 2371 3938 1207
Size: 444 720 1567 2335
Fragments arranged by size:
2335 1567 720 444
ApaI G_GGCC'C
Cuts at: 1700 1700
Size: 5066
ApaLI G'TGCA_C
Cuts at: 3836 3836
Size: 5066
ApoI r'AATT_y
Cuts at: 157 493 1131 1876 2070 4419 4675 157
Size: 336 638 745 194 2349 256 548
Fragments arranged by size:
2349 745 638 548 336 256 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 5066
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
AvaI C'yCGr_G
Cuts at: 2048 2086 2048
Size: 38 5028
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1646 1679 1646
Size: 33 5033
BamHI G'GATC_C
Cuts at: 2091 2091
Size: 5066
BanI G'GyrC_C
Cuts at: 1957 2082 2496 2744 2779 1957
Size: 125 414 248 35 4244
Fragments arranged by size:
4244 414 248 125 35
BanII G_rGCy'C
Cuts at: 1700 2055 2080 3110 1700
Size: 355 25 1030 3656
Fragments arranged by size:
3656 1030 355 25
BbeI G_GCGC'C
Cuts at: 2500 2748 2500
Size: 248 4818
BbsI GAAGACnn'nnnn_
Cuts at: 1229 1626 1817 1229
Size: 397 191 4478
Fragments arranged by size:
4478 397 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5032
BciVI GTATCCnnnnn_n'
Cuts at: 2964 3725 5066 2964
Size: 761 1341 2964
Fragments arranged by size:
2964 1341 761
BclI T'GATC_A
Cuts at: 2586 2586
Size: 5066
BglII A'GATC_T
Cuts at: 871 1708 2581 4618 871
Size: 837 873 2037 1319
Fragments arranged by size:
2037 1319 873 837
BlpI GC'TnA_GC
Cuts at: 2178 2178
Size: 5066
Bme1580I G_kGCm'C
Cuts at: 1700 2691 2784 3840 1700
Size: 991 93 1056 2926
Fragments arranged by size:
2926 1056 991 93
BmrI ACTGGGnnnn_n'
Cuts at: 2278 2359 2694 4838 2278
Size: 81 335 2144 2506
Fragments arranged by size:
2506 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 767 1082 3433 767
Size: 315 2351 2400
Fragments arranged by size:
2400 2351 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1802 1996 1802
Size: 194 4872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1379 2242 3121 3613 3911 1379
Size: 863 879 492 298 2534
Fragments arranged by size:
2534 879 863 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4770 4770
Size: 5066
BsaAI yAC'GTr
Cuts at: 2419 2419
Size: 5066
BsaBI GATnn'nnATC
Cuts at: 6 1049 2580 4617 6
Size: 1043 1531 2037 455
Fragments arranged by size:
2037 1531 1043 455
BsaHI Gr'CG_yC
Cuts at: 2497 2745 3447 4943 2497
Size: 248 702 1496 2620
Fragments arranged by size:
2620 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2255 2776 3454 3728 3875 4286 2255
Size: 521 678 274 147 411 3035
Fragments arranged by size:
3035 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 575 824 1058 575
Size: 249 234 4583
Fragments arranged by size:
4583 249 234
BseYI C'CCAG_C
Cuts at: 2491 3249 3826 4322 2491
Size: 758 577 496 3235
Fragments arranged by size:
3235 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2608 2654 3862 104
Size: 2504 46 1208 1308
Fragments arranged by size:
2504 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2080 2858 3048 3840 2080
Size: 778 190 792 3306
Fragments arranged by size:
3306 792 778 190
BsmI GAATG_Cn'
Cuts at: 603 603
Size: 5066
Bsp1286I G_dGCh'C
Cuts at: 1700 2055 2080 2691 2784 2858 3048 3110 3840
Size: 355 25 611 93 74 190 62 730
Cuts at: 3840 1700
Size: 2926
Fragments arranged by size:
2926 730 611 355 190 93 74 62
25
BspEI T'CCGG_A
Cuts at: 4286 4286
Size: 5066
BspHI T'CATG_A
Cuts at: 4242 4242
Size: 5066
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2116 2330 2632 3013 2116
Size: 214 302 381 4169
Fragments arranged by size:
4169 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3358 3412 4896 5061 50
Size: 3308 54 1484 165 55
Fragments arranged by size:
3308 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 448 2978 448
Size: 2530 2536
BsrFI r'CCGG_y
Cuts at: 3064 3245 3454 3064
Size: 181 209 4676
Fragments arranged by size:
4676 209 181
BssSI C'ACGA_G
Cuts at: 3337 3695 3337
Size: 358 4708
BstAPI GCAn_nnn'nTGC
Cuts at: 2443 2443
Size: 5066
BstBI TT'CG_AA
Cuts at: 1563 3427 1563
Size: 1864 3202
BstEII G'GTnAC_C
Cuts at: 607 607
Size: 5066
BstYI r'GATC_y
Cuts at: 1 871 1399 1708 2091 2558 2581 2916 3162
Size: 870 528 309 383 467 23 335 246
Cuts at: 3162 4174 4260 4272 4618 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 870 528 467 449 383 346 335
309 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3016 3197 3360 3016
Size: 181 163 4722
Fragments arranged by size:
4722 181 163
BtsI GCAGTG_nn'
Cuts at: 1161 2309 1161
Size: 1148 3918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 258 293 258
Size: 35 5031
DraI TTT'AAA
Cuts at: 1317 2065 1317
Size: 748 4318
DrdI GACnn_nn'nnGTC
Cuts at: 2772 3630 2772
Size: 858 4208
EaeI y'GGCC_r
Cuts at: 1998 2020 2651 2825 3216 3243 4588 1998
Size: 22 631 174 391 27 1345 2476
Fragments arranged by size:
2476 1345 631 391 174 27 22
EagI C'GGCC_G
Cuts at: 2651 2651
Size: 5066
EarI CTCTTCn'nnn_
Cuts at: 878 1895 3089 3299 878
Size: 1017 1194 210 2645
Fragments arranged by size:
2645 1194 1017 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3274 3584 3730 4876 3274
Size: 310 146 1146 3464
Fragments arranged by size:
3464 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2078 2078
Size: 5066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 545 711 724 896 1184 1229 1241 1802
Size: 165 166 13 172 288 45 12 561
Cuts at: 1802 1996 2891 3323 4070 4338 380
Size: 194 895 432 747 268 1108
Fragments arranged by size:
1108 895 747 561 432 288 268 194
172 166 165 45 13 12
EcoNI CCTnn'n_nnAGG
Cuts at: 839 839
Size: 5066
EcoO109I rG'GnC_Cy
Cuts at: 829 888 1357 1630 1648 2205 829
Size: 59 469 273 18 557 3690
Fragments arranged by size:
3690 557 469 273 59 18
EcoRI G'AATT_C
Cuts at: 2070 2070
Size: 5066
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1214 3085 1214
Size: 1871 3195
FspI TGC'GCA
Cuts at: 2847 2847
Size: 5066
HaeII r_GCGC'y
Cuts at: 2500 2748 3770 4749 2500
Size: 248 1022 979 2817
Fragments arranged by size:
2817 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 117 149 233 265 1462 1494 2908 2938 2940
Size: 32 84 32 1197 32 1414 30 2
Cuts at: 2940 2970 117
Size: 30 2213
Fragments arranged by size:
2213 1414 1197 84 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 228 644 2105 228
Size: 416 1461 3189
Fragments arranged by size:
3189 1461 416
HindIII A'AGCT_T
Cuts at: 324 1499 324
Size: 1175 3891
KasI G'GCGC_C
Cuts at: 2496 2744 2496
Size: 248 4818
KpnI G_GTAC'C
Cuts at: 2086 2086
Size: 5066
MluI A'CGCG_T
Cuts at: 4499 4499
Size: 5066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3737 3921 3737
Size: 184 4882
MscI TGG'CCA
Cuts at: 2000 2827 2000
Size: 827 4239
MslI CAynn'nnrTG
Cuts at: 668 1304 1532 3055 3182 668
Size: 636 228 1523 127 2552
Fragments arranged by size:
2552 1523 636 228 127
MspA1I CmG'CkG
Cuts at: 1660 2177 2491 2851 3864 4109 1660
Size: 517 314 360 1013 245 2617
Fragments arranged by size:
2617 1013 517 360 314 245
NaeI GCC'GGC
Cuts at: 3247 3247
Size: 5066
NarI GG'CG_CC
Cuts at: 2497 2745 2497
Size: 248 4818
NgoMIV G'CCGG_C
Cuts at: 3245 3245
Size: 5066
NspI r_CATG'y
Cuts at: 2119 2619 3526 2119
Size: 500 907 3659
Fragments arranged by size:
3659 907 500
PciI A'CATG_T
Cuts at: 3522 3522
Size: 5066
PmeI GTTT'AAAC
Cuts at: 2065 2065
Size: 5066
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4205 4237 4205
Size: 32 5034
PpuMI rG'GwC_Cy
Cuts at: 829 888 1357 1630 1648 829
Size: 59 469 273 18 4247
Fragments arranged by size:
4247 469 273 59 18
PsiI TTA'TAA
Cuts at: 1412 1412
Size: 5066
PspOMI G'GGCC_C
Cuts at: 1696 1696
Size: 5066
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1257 1289 1257
Size: 32 5034
PstI C_TGCA'G
Cuts at: 2113 2798 2113
Size: 685 4381
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5066
PvuII CAG'CTG
Cuts at: 1660 2491 2851 1660
Size: 831 360 3875
Fragments arranged by size:
3875 831 360
RsrII CG'GwC_CG
Cuts at: 3261 3261
Size: 5066
SacI G_AGCT'C
Cuts at: 2080 2080
Size: 5066
SalI G'TCGA_C
Cuts at: 2103 2103
Size: 5066
SapI GCTCTTCn'nnn_
Cuts at: 3089 3299 3089
Size: 210 4856
SbfI CC_TGCA'GG
Cuts at: 2113 2113
Size: 5066
ScaI AGT'ACT
Cuts at: 1194 4583 1194
Size: 3389 1677
SfcI C'TryA_G
Cuts at: 33 675 1403 2109 2794 3787 3978 33
Size: 642 728 706 685 993 191 1121
Fragments arranged by size:
1121 993 728 706 685 642 191
SfoI GGC'GCC
Cuts at: 2498 2746 2498
Size: 248 4818
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5066
SmaI CCC'GGG
Cuts at: 2050 2088 2050
Size: 38 5028
SmlI C'TyrA_G
Cuts at: 1394 2221 3136 3628 3890 1394
Size: 827 915 492 262 2570
Fragments arranged by size:
2570 915 827 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5066
SphI G_CATG'C
Cuts at: 2119 2619 2119
Size: 500 4566
SspI AAT'ATT
Cuts at: 351 1682 351
Size: 1331 3735
StyI C'CwwG_G
Cuts at: 2200 2554 2200
Size: 354 4712
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2649 2782 3241 3450 2649
Size: 133 459 209 4265
Fragments arranged by size:
4265 459 209 133
TatI w'GTAC_w
Cuts at: 1192 4581 1192
Size: 3389 1677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4583 4615 4583
Size: 32 5034
Tth111I GACn'n_nGTC
Cuts at: 2863 2863
Size: 5066
XbaI T'CTAG_A
Cuts at: 1252 2097 1252
Size: 845 4221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2561 2561
Size: 5066
XmaI C'CCGG_G
Cuts at: 2048 2086 2048
Size: 38 5028
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI