Restriction map for
pF1KE0014
(Circular) (Six-base) MAPSORT of: pF1KE0014.seq Check: 2587 from: 1 to: 4574
pF1KE0014 4574 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 208 208
Size: 4574
AccI GT'mk_AC
Cuts at: 1612 1612
Size: 4574
Acc65I G'GTAC_C
Cuts at: 1590 1590
Size: 4574
AclI AA'CG_TT
Cuts at: 4414 4414
Size: 4574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 902 2399 2831 3578 3846 388
Size: 514 1497 432 747 268 1116
Fragments arranged by size:
1497 1116 747 514 432 268
AflII C'TTAA_G
Cuts at: 1450 1450
Size: 4574
AflIII A'CryG_T
Cuts at: 1926 3030 4007 1926
Size: 1104 977 2493
Fragments arranged by size:
2493 1104 977
AgeI A'CCGG_T
Cuts at: 2962 2962
Size: 4574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 940 961 940
Size: 21 4553
AlwNI CAG_nnn'CTG
Cuts at: 183 231 1879 3446 183
Size: 48 1648 1567 1311
Fragments arranged by size:
1648 1567 1311 48
ApaI G_GGCC'C
Cuts at: 710 710
Size: 4574
ApaLI G'TGCA_C
Cuts at: 3344 3344
Size: 4574
ApoI r'AATT_y
Cuts at: 606 1267 1578 3927 4183 606
Size: 661 311 2349 256 997
Fragments arranged by size:
2349 997 661 311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4574
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
AvaI C'yCGr_G
Cuts at: 1594 1594
Size: 4574
BamHI G'GATC_C
Cuts at: 1599 1599
Size: 4574
BanI G'GyrC_C
Cuts at: 1102 1536 1590 2004 2252 2287 1102
Size: 434 54 414 248 35 3389
Fragments arranged by size:
3389 434 414 248 54 35
BanII G_rGCy'C
Cuts at: 710 1321 1588 2618 710
Size: 611 267 1030 2666
Fragments arranged by size:
2666 1030 611 267
BbeI G_GCGC'C
Cuts at: 2008 2256 2008
Size: 248 4326
BbsI GAAGACnn'nnnn_
Cuts at: 872 890 872
Size: 18 4556
BbvCI CC'TCA_GC
Cuts at: 117 117
Size: 4574
BciVI GTATCCnnnnn_n'
Cuts at: 484 1237 2472 3233 4574 484
Size: 753 1235 761 1341 484
Fragments arranged by size:
1341 1235 761 753 484
BclI T'GATC_A
Cuts at: 239 2094 239
Size: 1855 2719
BfrBI ATG'CAT
Cuts at: 992 992
Size: 4574
BglI GCCn_nnn'nGGC
Cuts at: 1141 1141
Size: 4574
BglII A'GATC_T
Cuts at: 2089 4126 2089
Size: 2037 2537
BlpI GC'TnA_GC
Cuts at: 184 847 1686 184
Size: 663 839 3072
Fragments arranged by size:
3072 839 663
Bme1580I G_kGCm'C
Cuts at: 710 2199 2292 3348 710
Size: 1489 93 1056 1936
Fragments arranged by size:
1936 1489 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 705 1786 1867 2202 4346 705
Size: 1081 81 335 2144 933
Fragments arranged by size:
2144 1081 933 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 4574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 1339
Size: 4574
Bpu10I CC'TnA_GC
Cuts at: 117 1327 1495 117
Size: 1210 168 3196
Fragments arranged by size:
3196 1210 168
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1150 1750 2629 3121 3419 784
Size: 366 600 879 492 298 1939
Fragments arranged by size:
1939 879 600 492 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 298 1204 4278 298
Size: 906 3074 594
Fragments arranged by size:
3074 906 594
BsaAI yAC'GTr
Cuts at: 643 1436 1927 643
Size: 793 491 3290
Fragments arranged by size:
3290 793 491
BsaBI GATnn'nnATC
Cuts at: 6 2088 4125 6
Size: 2082 2037 455
Fragments arranged by size:
2082 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 205 2005 2253 2955 4451 112
Size: 93 1800 248 702 1496 235
Fragments arranged by size:
1800 1496 702 248 235 93
BsaWI w'CCGG_w
Cuts at: 1763 2284 2962 3236 3383 3794 1763
Size: 521 678 274 147 411 2543
Fragments arranged by size:
2543 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 865 865
Size: 4574
BseYI C'CCAG_C
Cuts at: 1999 2757 3334 3830 1999
Size: 758 577 496 2743
Fragments arranged by size:
2743 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1219 763
Size: 456 4118
BsiEI CG_ry'CG
Cuts at: 104 2116 2162 3370 104
Size: 2012 46 1208 1308
Fragments arranged by size:
2012 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1588 2366 2556 3348 1588
Size: 778 190 792 2814
Fragments arranged by size:
2814 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 710 1321 1588 2199 2292 2366 2556 2618 3348
Size: 611 267 611 93 74 190 62 730
Cuts at: 3348 710
Size: 1936
Fragments arranged by size:
1936 730 611 611 267 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3794 3794
Size: 4574
BspHI T'CATG_A
Cuts at: 3750 3750
Size: 4574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1624 1838 2140 2521 1624
Size: 214 302 381 3677
Fragments arranged by size:
3677 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2866 2920 4404 4569 50
Size: 2816 54 1484 165 55
Fragments arranged by size:
2816 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1106 1538 2486 1106
Size: 432 948 3194
Fragments arranged by size:
3194 948 432
BsrFI r'CCGG_y
Cuts at: 508 2572 2753 2962 508
Size: 2064 181 209 2120
Fragments arranged by size:
2120 2064 209 181
BsrGI T'GTAC_A
Cuts at: 920 950 920
Size: 30 4544
BssSI C'ACGA_G
Cuts at: 2845 3203 2845
Size: 358 4216
BstAPI GCAn_nnn'nTGC
Cuts at: 1233 1951 1233
Size: 718 3856
BstBI TT'CG_AA
Cuts at: 2935 2935
Size: 4574
BstEII G'GTnAC_C
Cuts at: 259 259
Size: 4574
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 4574
BstYI r'GATC_y
Cuts at: 1 1599 2066 2089 2424 2670 3682 3768 3780
Size: 1598 467 23 335 246 1012 86 12
Cuts at: 3780 4126 1
Size: 346 449
Fragments arranged by size:
1598 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2524 2705 2868 2524
Size: 181 163 4230
Fragments arranged by size:
4230 181 163
BtsI GCAGTG_nn'
Cuts at: 440 500 841 1817 440
Size: 60 341 976 3197
Fragments arranged by size:
3197 976 341 60
DraI TTT'AAA
Cuts at: 1573 1573
Size: 4574
DraIII CAC_nnn'GTG
Cuts at: 544 544
Size: 4574
DrdI GACnn_nn'nnGTC
Cuts at: 2280 3138 2280
Size: 858 3716
EaeI y'GGCC_r
Cuts at: 1337 1479 2159 2333 2724 2751 4096 1337
Size: 142 680 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 680 391 174 142 27
EagI C'GGCC_G
Cuts at: 2159 2159
Size: 4574
EarI CTCTTCn'nnn_
Cuts at: 328 1402 1435 2597 2807 328
Size: 1074 33 1162 210 2095
Fragments arranged by size:
2095 1162 1074 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2782 3092 3238 4384 2782
Size: 310 146 1146 2972
Fragments arranged by size:
2972 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1586 1586
Size: 4574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 902 1339 2399 2831 3578 3846 388
Size: 514 437 1060 432 747 268 1116
Fragments arranged by size:
1116 1060 747 514 437 432 268
EcoO109I rG'GnC_Cy
Cuts at: 692 706 707 1713 692
Size: 14 1 1006 3553
Fragments arranged by size:
3553 1006 14 1
EcoRI G'AATT_C
Cuts at: 606 1267 1578 606
Size: 661 311 3602
Fragments arranged by size:
3602 661 311
EcoRV GAT'ATC
Cuts at: 522 522
Size: 4574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4574
FspI TGC'GCA
Cuts at: 2355 2355
Size: 4574
HaeII r_GCGC'y
Cuts at: 151 232 807 2008 2256 3278 4257 151
Size: 81 575 1201 248 1022 979 468
Fragments arranged by size:
1201 1022 979 575 468 248 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1235 1267 2416 2446 2448 2478 1235
Size: 32 1149 30 2 30 3331
Fragments arranged by size:
3331 1149 32 30 30 2
HincII GTy'rAC
Cuts at: 579 1613 579
Size: 1034 3540
KasI G'GCGC_C
Cuts at: 2004 2252 2004
Size: 248 4326
KpnI G_GTAC'C
Cuts at: 1594 1594
Size: 4574
MfeI C'AATT_G
Cuts at: 347 347
Size: 4574
MluI A'CGCG_T
Cuts at: 4007 4007
Size: 4574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3245 3429 3245
Size: 184 4390
MscI TGG'CCA
Cuts at: 1481 2335 1481
Size: 854 3720
MslI CAynn'nnrTG
Cuts at: 922 1066 2563 2690 922
Size: 144 1497 127 2806
Fragments arranged by size:
2806 1497 144 127
MspA1I CmG'CkG
Cuts at: 121 914 1685 1999 2359 3372 3617 121
Size: 793 771 314 360 1013 245 1078
Fragments arranged by size:
1078 1013 793 771 360 314 245
NaeI GCC'GGC
Cuts at: 2755 2755
Size: 4574
NarI GG'CG_CC
Cuts at: 2005 2253 2005
Size: 248 4326
NcoI C'CATG_G
Cuts at: 107 107
Size: 4574
NgoMIV G'CCGG_C
Cuts at: 2753 2753
Size: 4574
NsiI A_TGCA'T
Cuts at: 994 994
Size: 4574
NspI r_CATG'y
Cuts at: 921 927 1627 2127 3034 921
Size: 6 700 500 907 2461
Fragments arranged by size:
2461 907 700 500 6
PasI CC'CwG_GG
Cuts at: 210 210
Size: 4574
PciI A'CATG_T
Cuts at: 3030 3030
Size: 4574
PflMI CCAn_nnn'nTGG
Cuts at: 1136 1391 1136
Size: 255 4319
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 4574
PmeI GTTT'AAAC
Cuts at: 1573 1573
Size: 4574
PmlI CAC'GTG
Cuts at: 1436 1436
Size: 4574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 4542
PspOMI G'GGCC_C
Cuts at: 706 706
Size: 4574
PstI C_TGCA'G
Cuts at: 167 1621 2306 167
Size: 1454 685 2435
Fragments arranged by size:
2435 1454 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4574
PvuII CAG'CTG
Cuts at: 1999 2359 1999
Size: 360 4214
RsrII CG'GwC_CG
Cuts at: 2769 2769
Size: 4574
SacI G_AGCT'C
Cuts at: 1588 1588
Size: 4574
SalI G'TCGA_C
Cuts at: 1611 1611
Size: 4574
SapI GCTCTTCn'nnn_
Cuts at: 1402 2597 2807 1402
Size: 1195 210 3169
Fragments arranged by size:
3169 1195 210
SbfI CC_TGCA'GG
Cuts at: 1621 1621
Size: 4574
ScaI AGT'ACT
Cuts at: 4091 4091
Size: 4574
SexAI A'CCwGG_T
Cuts at: 1247 1247
Size: 4574
SfcI C'TryA_G
Cuts at: 33 163 1617 2302 3295 3486 33
Size: 130 1454 685 993 191 1121
Fragments arranged by size:
1454 1121 993 685 191 130
SfoI GGC'GCC
Cuts at: 2006 2254 2006
Size: 248 4326
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4574
SmaI CCC'GGG
Cuts at: 1596 1596
Size: 4574
SmlI C'TyrA_G
Cuts at: 799 1165 1450 1729 2644 3136 3398 799
Size: 366 285 279 915 492 262 1975
Fragments arranged by size:
1975 915 492 366 285 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4574
SphI G_CATG'C
Cuts at: 1627 2127 1627
Size: 500 4074
StuI AGG'CCT
Cuts at: 341 341
Size: 4574
StyI C'CwwG_G
Cuts at: 107 1173 1476 1540 1708 2062 107
Size: 1066 303 64 168 354 2619
Fragments arranged by size:
2619 1066 354 303 168 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1128 2157 2290 2749 2958 1128
Size: 1029 133 459 209 2744
Fragments arranged by size:
2744 1029 459 209 133
TatI w'GTAC_w
Cuts at: 920 950 4089 920
Size: 30 3139 1405
Fragments arranged by size:
3139 1405 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4091 4123 4091
Size: 32 4542
Tth111I GACn'n_nGTC
Cuts at: 2371 2371
Size: 4574
XbaI T'CTAG_A
Cuts at: 1605 1605
Size: 4574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1137 2069 1137
Size: 932 3642
XmaI C'CCGG_G
Cuts at: 1594 1594
Size: 4574
ZraI GAC'GTC
Cuts at: 206 206
Size: 4574
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AloI AscI AvrII BaeI
BcgI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BssHII
BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HindIII
HpaI NdeI NheI NotI NruI PacI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI