Restriction map for
pF1KE0009
(Circular) (Six-base) MAPSORT of: pF1KE0009.seq Check: 1731 from: 1 to: 5042
pF1KE0009 5042 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2080 2080
Size: 5042
Acc65I G'GTAC_C
Cuts at: 2058 2058
Size: 5042
AclI AA'CG_TT
Cuts at: 4882 4882
Size: 5042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 1169 1237 1303 2867 3299 4046 4314 575
Size: 594 68 66 1564 432 747 268 1303
Fragments arranged by size:
1564 1303 747 594 432 268 68 66
AflIII A'CryG_T
Cuts at: 2394 3498 4475 2394
Size: 1104 977 2961
Fragments arranged by size:
2961 1104 977
AgeI A'CCGG_T
Cuts at: 3430 3430
Size: 5042
AlwNI CAG_nnn'CTG
Cuts at: 1987 2347 3914 1987
Size: 360 1567 3115
Fragments arranged by size:
3115 1567 360
ApaLI G'TGCA_C
Cuts at: 3812 3812
Size: 5042
ApoI r'AATT_y
Cuts at: 179 269 1143 1465 1547 2046 4395 4651 179
Size: 90 874 322 82 499 2349 256 570
Fragments arranged by size:
2349 874 570 499 322 256 90 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 5042
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5042
AvaI C'yCGr_G
Cuts at: 2062 2062
Size: 5042
AvrII C'CTAG_G
Cuts at: 184 184
Size: 5042
BamHI G'GATC_C
Cuts at: 1751 2067 1751
Size: 316 4726
BanI G'GyrC_C
Cuts at: 533 2058 2472 2720 2755 533
Size: 1525 414 248 35 2820
Fragments arranged by size:
2820 1525 414 248 35
BanII G_rGCy'C
Cuts at: 467 1091 2056 3086 467
Size: 624 965 1030 2423
Fragments arranged by size:
2423 1030 965 624
BbeI G_GCGC'C
Cuts at: 2476 2724 2476
Size: 248 4794
BbsI GAAGACnn'nnnn_
Cuts at: 1480 1480
Size: 5042
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1086 1120 1086
Size: 34 5008
BciVI GTATCCnnnnn_n'
Cuts at: 1913 2940 3701 5042 1913
Size: 1027 761 1341 1913
Fragments arranged by size:
1913 1341 1027 761
BclI T'GATC_A
Cuts at: 2562 2562
Size: 5042
BglII A'GATC_T
Cuts at: 203 2557 4594 203
Size: 2354 2037 651
Fragments arranged by size:
2354 2037 651
BlpI GC'TnA_GC
Cuts at: 2154 2154
Size: 5042
Bme1580I G_kGCm'C
Cuts at: 538 2667 2760 3816 538
Size: 2129 93 1056 1764
Fragments arranged by size:
2129 1764 1056 93
BmgBI CAC'GTC
Cuts at: 1689 1689
Size: 5042
BmrI ACTGGGnnnn_n'
Cuts at: 2254 2335 2670 4814 2254
Size: 81 335 2144 2482
Fragments arranged by size:
2482 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3409 3409
Size: 5042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 824 1105 389
Size: 435 281 4326
Fragments arranged by size:
4326 435 281
Bpu10I CC'TnA_GC
Cuts at: 461 461
Size: 5042
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2218 3097 3589 3887 2218
Size: 879 492 298 3373
Fragments arranged by size:
3373 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 721 4746 721
Size: 4025 1017
BsaAI yAC'GTr
Cuts at: 2395 2395
Size: 5042
BsaBI GATnn'nnATC
Cuts at: 6 2556 4593 6
Size: 2550 2037 455
Fragments arranged by size:
2550 2037 455
BsaHI Gr'CG_yC
Cuts at: 2473 2721 3423 4919 2473
Size: 248 702 1496 2596
Fragments arranged by size:
2596 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2231 2752 3430 3704 3851 4262 2231
Size: 521 678 274 147 411 3011
Fragments arranged by size:
3011 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 1828 1858 328
Size: 30 1470 30 3512
Fragments arranged by size:
3512 1470 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 623 697 794 797 823 828 865 868 871
Size: 74 97 3 26 5 37 3 3
Cuts at: 871 880 883 886 895 904 913 922 938
Size: 9 3 3 9 9 9 9 16
Cuts at: 938 956 623
Size: 18 4709
Fragments arranged by size:
4709 97 74 37 26 18 16 9
9 9 9 9 5 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2467 3225 3802 4298 2467
Size: 758 577 496 3211
Fragments arranged by size:
3211 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 1702 453
Size: 1249 3793
BsiEI CG_ry'CG
Cuts at: 104 2584 2630 3838 104
Size: 2480 46 1208 1308
Fragments arranged by size:
2480 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1091 2056 2834 3024 3816 1003
Size: 88 965 778 190 792 2229
Fragments arranged by size:
2229 965 792 778 190 88
BsmI GAATG_Cn'
Cuts at: 568 944 1204 568
Size: 376 260 4406
Fragments arranged by size:
4406 376 260
Bsp1286I G_dGCh'C
Cuts at: 467 538 1003 1091 2056 2667 2760 2834 3024
Size: 71 465 88 965 611 93 74 190
Cuts at: 3024 3086 3816 467
Size: 62 730 1693
Fragments arranged by size:
1693 965 730 611 465 190 93 88
74 71 62
BspEI T'CCGG_A
Cuts at: 4262 4262
Size: 5042
BspHI T'CATG_A
Cuts at: 4218 4218
Size: 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 484 2092 2306 2608 2989 484
Size: 1608 214 302 381 2537
Fragments arranged by size:
2537 1608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3334 3388 4872 5037 50
Size: 3284 54 1484 165 55
Fragments arranged by size:
3284 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2954 2954
Size: 5042
BsrFI r'CCGG_y
Cuts at: 3040 3221 3430 3040
Size: 181 209 4652
Fragments arranged by size:
4652 209 181
BsrGI T'GTAC_A
Cuts at: 437 437
Size: 5042
BssSI C'ACGA_G
Cuts at: 3313 3671 3313
Size: 358 4684
BstAPI GCAn_nnn'nTGC
Cuts at: 476 2419 476
Size: 1943 3099
BstBI TT'CG_AA
Cuts at: 3403 3403
Size: 5042
BstEII G'GTnAC_C
Cuts at: 1362 1362
Size: 5042
BstYI r'GATC_y
Cuts at: 1 203 1751 2067 2534 2557 2892 3138 4150
Size: 202 1548 316 467 23 335 246 1012
Cuts at: 4150 4236 4248 4594 1
Size: 86 12 346 449
Fragments arranged by size:
1548 1012 467 449 346 335 316 246
202 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 635 635
Size: 5042
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1089 2992 3173 3336 1089
Size: 1903 181 163 2795
Fragments arranged by size:
2795 1903 181 163
BtsI GCAGTG_nn'
Cuts at: 979 2285 979
Size: 1306 3736
ClaI AT'CG_AT
Cuts at: 1328 1328
Size: 5042
DraI TTT'AAA
Cuts at: 2041 2041
Size: 5042
DrdI GACnn_nn'nnGTC
Cuts at: 2748 3606 2748
Size: 858 4184
EaeI y'GGCC_r
Cuts at: 1770 2627 2801 3192 3219 4564 1770
Size: 857 174 391 27 1345 2248
Fragments arranged by size:
2248 1345 857 391 174 27
EagI C'GGCC_G
Cuts at: 2627 2627
Size: 5042
EarI CTCTTCn'nnn_
Cuts at: 489 1426 3065 3275 489
Size: 937 1639 210 2256
Fragments arranged by size:
2256 1639 937 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3250 3560 3706 4852 3250
Size: 310 146 1146 3440
Fragments arranged by size:
3440 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1089 2054 1089
Size: 965 4077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 575 824 1105 1169 1237 1303 2867 3299
Size: 186 249 281 64 68 66 1564 432
Cuts at: 3299 4046 4314 389
Size: 747 268 1117
Fragments arranged by size:
1564 1117 747 432 281 268 249 186
68 66 64
EcoNI CCTnn'n_nnAGG
Cuts at: 971 971
Size: 5042
EcoO109I rG'GnC_Cy
Cuts at: 912 2181 912
Size: 1269 3773
EcoRI G'AATT_C
Cuts at: 269 2046 269
Size: 1777 3265
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 688 3061 688
Size: 2373 2669
FspI TGC'GCA
Cuts at: 2823 2823
Size: 5042
HaeII r_GCGC'y
Cuts at: 2476 2724 3746 4725 2476
Size: 248 1022 979 2793
Fragments arranged by size:
2793 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1065 1097 1828 1860 1888 1920 2884 2914 2916
Size: 32 731 32 28 32 964 30 2
Cuts at: 2916 2946 1065
Size: 30 3161
Fragments arranged by size:
3161 964 731 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 318 2081 318
Size: 1763 3279
HindIII A'AGCT_T
Cuts at: 1210 1210
Size: 5042
HpaI GTT'AAC
Cuts at: 318 318
Size: 5042
KasI G'GCGC_C
Cuts at: 2472 2720 2472
Size: 248 4794
KpnI G_GTAC'C
Cuts at: 2062 2062
Size: 5042
MfeI C'AATT_G
Cuts at: 1273 1273
Size: 5042
MluI A'CGCG_T
Cuts at: 4475 4475
Size: 5042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3713 3897 3713
Size: 184 4858
MscI TGG'CCA
Cuts at: 1772 2803 1772
Size: 1031 4011
MslI CAynn'nnrTG
Cuts at: 433 3031 3158 433
Size: 2598 127 2317
Fragments arranged by size:
2598 2317 127
MspA1I CmG'CkG
Cuts at: 1025 1059 2153 2467 2827 3840 4085 1025
Size: 34 1094 314 360 1013 245 1982
Fragments arranged by size:
1982 1094 1013 360 314 245 34
NaeI GCC'GGC
Cuts at: 3223 3223
Size: 5042
NarI GG'CG_CC
Cuts at: 2473 2721 2473
Size: 248 4794
NgoMIV G'CCGG_C
Cuts at: 3221 3221
Size: 5042
NspI r_CATG'y
Cuts at: 2095 2595 3502 2095
Size: 500 907 3635
Fragments arranged by size:
3635 907 500
PciI A'CATG_T
Cuts at: 3498 3498
Size: 5042
PfoI T'CCnGG_A
Cuts at: 366 366
Size: 5042
PmeI GTTT'AAAC
Cuts at: 2041 2041
Size: 5042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 213 245 4181 4213 213
Size: 32 3936 32 1042
Fragments arranged by size:
3936 1042 32 32
PpuMI rG'GwC_Cy
Cuts at: 912 912
Size: 5042
PsiI TTA'TAA
Cuts at: 386 1595 386
Size: 1209 3833
PstI C_TGCA'G
Cuts at: 2089 2774 2089
Size: 685 4357
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5042
PvuII CAG'CTG
Cuts at: 1025 2467 2827 1025
Size: 1442 360 3240
Fragments arranged by size:
3240 1442 360
RsrII CG'GwC_CG
Cuts at: 3237 3237
Size: 5042
SacI G_AGCT'C
Cuts at: 1091 2056 1091
Size: 965 4077
SalI G'TCGA_C
Cuts at: 2079 2079
Size: 5042
SapI GCTCTTCn'nnn_
Cuts at: 1426 3065 3275 1426
Size: 1639 210 3193
Fragments arranged by size:
3193 1639 210
SbfI CC_TGCA'GG
Cuts at: 2089 2089
Size: 5042
ScaI AGT'ACT
Cuts at: 1727 4559 1727
Size: 2832 2210
SfcI C'TryA_G
Cuts at: 33 1011 2085 2770 3763 3954 33
Size: 978 1074 685 993 191 1121
Fragments arranged by size:
1121 1074 993 978 685 191
SfoI GGC'GCC
Cuts at: 2474 2722 2474
Size: 248 4794
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5042
SmaI CCC'GGG
Cuts at: 2064 2064
Size: 5042
SmlI C'TyrA_G
Cuts at: 2197 3112 3604 3866 2197
Size: 915 492 262 3373
Fragments arranged by size:
3373 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5042
SphI G_CATG'C
Cuts at: 2095 2595 2095
Size: 500 4542
StuI AGG'CCT
Cuts at: 801 1787 801
Size: 986 4056
StyI C'CwwG_G
Cuts at: 184 840 1731 2176 2530 184
Size: 656 891 445 354 2696
Fragments arranged by size:
2696 891 656 445 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 665 2625 2758 3217 3426 665
Size: 1960 133 459 209 2281
Fragments arranged by size:
2281 1960 459 209 133
TatI w'GTAC_w
Cuts at: 437 1584 1725 4557 437
Size: 1147 141 2832 922
Fragments arranged by size:
2832 1147 922 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1678 1710 4559 4591 1678
Size: 32 2849 32 2129
Fragments arranged by size:
2849 2129 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 2839 1840
Size: 999 4043
XbaI T'CTAG_A
Cuts at: 739 1650 1992 2073 739
Size: 911 342 81 3708
Fragments arranged by size:
3708 911 342 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2537 2537
Size: 5042
XmaI C'CCGG_G
Cuts at: 2062 2062
Size: 5042
XmnI GAAnn'nnTTC
Cuts at: 179 671 1880 179
Size: 492 1209 3341
Fragments arranged by size:
3341 1209 492
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BfrBI BglI BmtI BsiWI
BsmBI BssHII BstXI BstZ17I BtgI CspCI DraIII EcoRV
FseI FspAI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI