Restriction map for
pF1KE0008
(Circular) (Six-base) MAPSORT of: pF1KE0008.seq Check: 6458 from: 1 to: 5204
pF1KE0008 5204 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1642 1642
Size: 5204
AccI GT'mk_AC
Cuts at: 1438 2242 1438
Size: 804 4400
Acc65I G'GTAC_C
Cuts at: 2220 2220
Size: 5204
AclI AA'CG_TT
Cuts at: 5044 5044
Size: 5204
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 3029 3461 4208 4476 185
Size: 2844 432 747 268 913
Fragments arranged by size:
2844 913 747 432 268
AflIII A'CryG_T
Cuts at: 1413 2556 3660 4637 1413
Size: 1143 1104 977 1980
Fragments arranged by size:
1980 1143 1104 977
AgeI A'CCGG_T
Cuts at: 3592 3592
Size: 5204
AhdI GACnn_n'nnGTC
Cuts at: 904 904
Size: 5204
AlwNI CAG_nnn'CTG
Cuts at: 853 2509 4076 853
Size: 1656 1567 1981
Fragments arranged by size:
1981 1656 1567
ApaLI G'TGCA_C
Cuts at: 3974 3974
Size: 5204
ApoI r'AATT_y
Cuts at: 288 533 580 887 1013 1247 1428 1749 2208
Size: 245 47 307 126 234 181 321 459
Cuts at: 2208 4557 4813 288
Size: 2349 256 679
Fragments arranged by size:
2349 679 459 321 307 256 245 234
181 126 47
AseI AT'TA_AT
Cuts at: 20 1784 20
Size: 1764 3440
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
AvaI C'yCGr_G
Cuts at: 2224 2224
Size: 5204
BamHI G'GATC_C
Cuts at: 2229 2229
Size: 5204
BanI G'GyrC_C
Cuts at: 2220 2634 2882 2917 2220
Size: 414 248 35 4507
Fragments arranged by size:
4507 414 248 35
BanII G_rGCy'C
Cuts at: 1304 1647 2218 3248 1304
Size: 343 571 1030 3260
Fragments arranged by size:
3260 1030 571 343
BbeI G_GCGC'C
Cuts at: 2638 2886 2638
Size: 248 4956
BbsI GAAGACnn'nnnn_
Cuts at: 903 1189 903
Size: 286 4918
BciVI GTATCCnnnnn_n'
Cuts at: 3102 3863 5204 3102
Size: 761 1341 3102
Fragments arranged by size:
3102 1341 761
BclI T'GATC_A
Cuts at: 2724 2724
Size: 5204
BfrBI ATG'CAT
Cuts at: 195 195
Size: 5204
BglII A'GATC_T
Cuts at: 2719 4756 2719
Size: 2037 3167
BlpI GC'TnA_GC
Cuts at: 2316 2316
Size: 5204
Bme1580I G_kGCm'C
Cuts at: 1622 2829 2922 3978 1622
Size: 1207 93 1056 2848
Fragments arranged by size:
2848 1207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1890 2416 2497 2832 4976 1890
Size: 526 81 335 2144 2118
Fragments arranged by size:
2144 2118 526 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3571 3571
Size: 5204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 607
Size: 5204
Bpu10I CC'TnA_GC
Cuts at: 255 255
Size: 5204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1275 2380 3259 3751 4049 1275
Size: 1105 879 492 298 2430
Fragments arranged by size:
2430 1105 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1483 4908 1483
Size: 3425 1779
BsaAI yAC'GTr
Cuts at: 267 1598 2557 267
Size: 1331 959 2914
Fragments arranged by size:
2914 1331 959
BsaBI GATnn'nnATC
Cuts at: 6 2718 4755 6
Size: 2712 2037 455
Fragments arranged by size:
2712 2037 455
BsaHI Gr'CG_yC
Cuts at: 2635 2883 3585 5081 2635
Size: 248 702 1496 2758
Fragments arranged by size:
2758 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2393 2914 3592 3866 4013 4424 2393
Size: 521 678 274 147 411 3173
Fragments arranged by size:
3173 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1885 1915 1885
Size: 30 5174
BseYI C'CCAG_C
Cuts at: 2629 3387 3964 4460 2629
Size: 758 577 496 3373
Fragments arranged by size:
3373 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2746 2792 4000 104
Size: 2642 46 1208 1308
Fragments arranged by size:
2642 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 520 2218 2996 3186 3978 520
Size: 1698 778 190 792 1746
Fragments arranged by size:
1746 1698 792 778 190
BsmI GAATG_Cn'
Cuts at: 195 952 1539 195
Size: 757 587 3860
Fragments arranged by size:
3860 757 587
Bsp1286I G_dGCh'C
Cuts at: 520 1304 1622 1647 2218 2829 2922 2996 3186
Size: 784 318 25 571 611 93 74 190
Cuts at: 3186 3248 3978 520
Size: 62 730 1746
Fragments arranged by size:
1746 784 730 611 571 318 190 93
74 62 25
BspEI T'CCGG_A
Cuts at: 4424 4424
Size: 5204
BspHI T'CATG_A
Cuts at: 4380 4380
Size: 5204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1642 2254 2468 2770 3151 1642
Size: 612 214 302 381 3695
Fragments arranged by size:
3695 612 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3496 3550 5034 5199 50
Size: 3446 54 1484 165 55
Fragments arranged by size:
3446 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 605 3116 161
Size: 444 2511 2249
Fragments arranged by size:
2511 2249 444
BsrFI r'CCGG_y
Cuts at: 3202 3383 3592 3202
Size: 181 209 4814
Fragments arranged by size:
4814 209 181
BsrGI T'GTAC_A
Cuts at: 1416 1416
Size: 5204
BssSI C'ACGA_G
Cuts at: 3475 3833 3475
Size: 358 4846
BstAPI GCAn_nnn'nTGC
Cuts at: 2581 2581
Size: 5204
BstBI TT'CG_AA
Cuts at: 3565 3565
Size: 5204
BstYI r'GATC_y
Cuts at: 1 2099 2229 2696 2719 3054 3300 4312 4398
Size: 2098 130 467 23 335 246 1012 86
Cuts at: 4398 4410 4756 1
Size: 12 346 449
Fragments arranged by size:
2098 1012 467 449 346 335 246 130
86 23 12
BtgI C'CryG_G
Cuts at: 879 1289 1626 879
Size: 410 337 4457
Fragments arranged by size:
4457 410 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2021 3154 3335 3498 2021
Size: 1133 181 163 3727
Fragments arranged by size:
3727 1133 181 163
BtsI GCAGTG_nn'
Cuts at: 1396 1808 2447 1396
Size: 412 639 4153
Fragments arranged by size:
4153 639 412
DraI TTT'AAA
Cuts at: 450 2203 450
Size: 1753 3451
DrdI GACnn_nn'nnGTC
Cuts at: 2910 3768 2910
Size: 858 4346
EaeI y'GGCC_r
Cuts at: 2012 2789 2963 3354 3381 4726 2012
Size: 777 174 391 27 1345 2490
Fragments arranged by size:
2490 1345 777 391 174 27
EagI C'GGCC_G
Cuts at: 2789 2789
Size: 5204
EarI CTCTTCn'nnn_
Cuts at: 1309 3227 3437 1309
Size: 1918 210 3076
Fragments arranged by size:
3076 1918 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3412 3722 3868 5014 3412
Size: 310 146 1146 3602
Fragments arranged by size:
3602 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 5204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 607 3029 3461 4208 4476 185
Size: 422 2422 432 747 268 913
Fragments arranged by size:
2422 913 747 432 422 268
EcoO109I rG'GnC_Cy
Cuts at: 2343 2343
Size: 5204
EcoRI G'AATT_C
Cuts at: 2208 2208
Size: 5204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 5204
FspI TGC'GCA
Cuts at: 2985 2985
Size: 5204
HaeII r_GCGC'y
Cuts at: 2638 2886 3908 4887 2638
Size: 248 1022 979 2955
Fragments arranged by size:
2955 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 669 701 798 830 890 922 1014
Size: 32 210 32 97 32 60 32 92
Cuts at: 1014 1046 3046 3076 3078 3108 427
Size: 32 2000 30 2 30 2523
Fragments arranged by size:
2523 2000 210 97 92 60 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2243 2243
Size: 5204
HindIII A'AGCT_T
Cuts at: 1553 1553
Size: 5204
KasI G'GCGC_C
Cuts at: 2634 2882 2634
Size: 248 4956
KpnI G_GTAC'C
Cuts at: 2224 2224
Size: 5204
MluI A'CGCG_T
Cuts at: 4637 4637
Size: 5204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 576 1323 3875 4059 576
Size: 747 2552 184 1721
Fragments arranged by size:
2552 1721 747 184
MscI TGG'CCA
Cuts at: 2014 2965 2014
Size: 951 4253
MslI CAynn'nnrTG
Cuts at: 619 1625 3193 3320 619
Size: 1006 1568 127 2503
Fragments arranged by size:
2503 1568 1006 127
MspA1I CmG'CkG
Cuts at: 2315 2629 2989 4002 4247 2315
Size: 314 360 1013 245 3272
Fragments arranged by size:
3272 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3385 3385
Size: 5204
NarI GG'CG_CC
Cuts at: 2635 2883 2635
Size: 248 4956
NcoI C'CATG_G
Cuts at: 1289 1626 1289
Size: 337 4867
NdeI CA'TA_TG
Cuts at: 1481 1481
Size: 5204
NgoMIV G'CCGG_C
Cuts at: 3383 3383
Size: 5204
NsiI A_TGCA'T
Cuts at: 197 197
Size: 5204
NspI r_CATG'y
Cuts at: 195 1263 1417 1449 2257 2757 3664 195
Size: 1068 154 32 808 500 907 1735
Fragments arranged by size:
1735 1068 907 808 500 154 32
PciI A'CATG_T
Cuts at: 1413 3660 1413
Size: 2247 2957
PflMI CCAn_nnn'nTGG
Cuts at: 398 1243 1314 2021 398
Size: 845 71 707 3581
Fragments arranged by size:
3581 845 707 71
PmeI GTTT'AAAC
Cuts at: 2203 2203
Size: 5204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 4343
Size: 32 5172
PstI C_TGCA'G
Cuts at: 1378 2251 2936 1378
Size: 873 685 3646
Fragments arranged by size:
3646 873 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5204
PvuII CAG'CTG
Cuts at: 2629 2989 2629
Size: 360 4844
RsrII CG'GwC_CG
Cuts at: 3399 3399
Size: 5204
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 5204
SalI G'TCGA_C
Cuts at: 2241 2241
Size: 5204
SapI GCTCTTCn'nnn_
Cuts at: 1309 3227 3437 1309
Size: 1918 210 3076
Fragments arranged by size:
3076 1918 210
SbfI CC_TGCA'GG
Cuts at: 2251 2251
Size: 5204
ScaI AGT'ACT
Cuts at: 4721 4721
Size: 5204
SfcI C'TryA_G
Cuts at: 33 1374 2247 2932 3925 4116 33
Size: 1341 873 685 993 191 1121
Fragments arranged by size:
1341 1121 993 873 685 191
SfoI GGC'GCC
Cuts at: 2636 2884 2636
Size: 248 4956
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5204
SmaI CCC'GGG
Cuts at: 2226 2226
Size: 5204
SmlI C'TyrA_G
Cuts at: 1254 2359 3274 3766 4028 1254
Size: 1105 915 492 262 2430
Fragments arranged by size:
2430 1105 915 492 262
SnaBI TAC'GTA
Cuts at: 267 1598 267
Size: 1331 3873
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5204
SphI G_CATG'C
Cuts at: 1449 2257 2757 1449
Size: 808 500 3896
Fragments arranged by size:
3896 808 500
SspI AAT'ATT
Cuts at: 481 481
Size: 5204
StyI C'CwwG_G
Cuts at: 1289 1626 1759 2338 2692 1289
Size: 337 133 579 354 3801
Fragments arranged by size:
3801 579 354 337 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1639 1752 2787 2920 3379 3588 1639
Size: 113 1035 133 459 209 3255
Fragments arranged by size:
3255 1035 459 209 133 113
TatI w'GTAC_w
Cuts at: 1416 4719 1416
Size: 3303 1901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1925 1957 1994 2026 4721 4753 1925
Size: 32 37 32 2695 32 2376
Fragments arranged by size:
2695 2376 37 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3001 3001
Size: 5204
XbaI T'CTAG_A
Cuts at: 2235 2235
Size: 5204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2699 2699
Size: 5204
XmaI C'CCGG_G
Cuts at: 2224 2224
Size: 5204
XmnI GAAnn'nnTTC
Cuts at: 700 900 700
Size: 200 5004
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI