Restriction map for
pF1KE0003
(Circular) (Six-base) MAPSORT of: pF1KE0003.seq Check: 7369 from: 1 to: 4655
pF1KE0003 4655 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 739 739
Size: 4655
AccI GT'mk_AC
Cuts at: 1538 1693 1538
Size: 155 4500
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 1649 4495 1649
Size: 2846 1809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 619 1457 2480 2912 3659 3927 429
Size: 190 838 1023 432 747 268 1157
Fragments arranged by size:
1157 1023 838 747 432 268 190
AflIII A'CryG_T
Cuts at: 1241 2007 3111 4088 1241
Size: 766 1104 977 1808
Fragments arranged by size:
1808 1104 977 766
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AlwNI CAG_nnn'CTG
Cuts at: 1609 1960 3527 1609
Size: 351 1567 2737
Fragments arranged by size:
2737 1567 351
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 358 883 1659 4008 4264 358
Size: 525 776 2349 256 749
Fragments arranged by size:
2349 776 749 525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4655
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1113 1454 1675 1113
Size: 341 221 4093
Fragments arranged by size:
4093 341 221
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 1671 2085 2333 2368 1671
Size: 414 248 35 3958
Fragments arranged by size:
3958 414 248 35
BanII G_rGCy'C
Cuts at: 1576 1669 2699 1576
Size: 93 1030 3532
Fragments arranged by size:
3532 1030 93
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 1487 1538 1487
Size: 51 4604
BciVI GTATCCnnnnn_n'
Cuts at: 2553 3314 4655 2553
Size: 761 1341 2553
Fragments arranged by size:
2553 1341 761
BclI T'GATC_A
Cuts at: 1611 2175 1611
Size: 564 4091
BglII A'GATC_T
Cuts at: 2170 4207 2170
Size: 2037 2618
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 2280 2373 3429 2280
Size: 93 1056 3506
Fragments arranged by size:
3506 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 202 666 800 888 1867 1948 2283 4427 202
Size: 464 134 88 979 81 335 2144 430
Fragments arranged by size:
2144 979 464 430 335 134 88 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 318
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1590 1831 2710 3202 3500 1590
Size: 241 879 492 298 2745
Fragments arranged by size:
2745 879 492 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 152 4359 152
Size: 4207 448
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 914 2169 4206 6
Size: 908 1255 2037 455
Fragments arranged by size:
2037 1255 908 455
BsaHI Gr'CG_yC
Cuts at: 736 2086 2334 3036 4532 736
Size: 1350 248 702 1496 859
Fragments arranged by size:
1496 1350 859 702 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3317 3464 3875 1844
Size: 521 678 274 147 411 2624
Fragments arranged by size:
2624 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 302
Size: 4655
BseYI C'CCAG_C
Cuts at: 776 2080 2838 3415 3911 776
Size: 1304 758 577 496 1520
Fragments arranged by size:
1520 1304 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1576 1669 2447 2637 3429 1576
Size: 93 778 190 792 2802
Fragments arranged by size:
2802 792 778 190 93
BsmI GAATG_Cn'
Cuts at: 826 826
Size: 4655
Bsp1286I G_dGCh'C
Cuts at: 1576 1669 2280 2373 2447 2637 2699 3429 1576
Size: 93 611 93 74 190 62 730 2802
Fragments arranged by size:
2802 730 611 190 93 93 74 62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 563 1089 3831 563
Size: 526 2742 1387
Fragments arranged by size:
2742 1387 526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1247 1705 1919 2221 2602 1247
Size: 458 214 302 381 3300
Fragments arranged by size:
3300 458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 832 2032 832
Size: 1200 3455
BstBI TT'CG_AA
Cuts at: 3016 3016
Size: 4655
BstYI r'GATC_y
Cuts at: 1 1050 1680 2147 2170 2505 2751 3763 3849
Size: 1049 630 467 23 335 246 1012 86
Cuts at: 3849 3861 4207 1
Size: 12 346 449
Fragments arranged by size:
1049 1012 630 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2605 2786 2949 2605
Size: 181 163 4311
Fragments arranged by size:
4311 181 163
BtsI GCAGTG_nn'
Cuts at: 1898 1898
Size: 4655
DraI TTT'AAA
Cuts at: 1154 1654 1154
Size: 500 4155
DrdI GACnn_nn'nnGTC
Cuts at: 2361 3219 2361
Size: 858 3797
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 469 2678 2888 469
Size: 2209 210 2236
Fragments arranged by size:
2236 2209 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1574 1667 1574
Size: 93 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 429 619 1457 2480 2912 3659 3927 318
Size: 111 190 838 1023 432 747 268 1046
Fragments arranged by size:
1046 1023 838 747 432 268 190 111
EcoO109I rG'GnC_Cy
Cuts at: 204 584 1794 204
Size: 380 1210 3065
Fragments arranged by size:
3065 1210 380
EcoRI G'AATT_C
Cuts at: 883 1659 883
Size: 776 3879
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 4655
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 509 541 1035 1067 1182 1214 2497
Size: 32 68 32 494 32 115 32 1283
Cuts at: 2497 2527 2529 2559 409
Size: 30 2 30 2505
Fragments arranged by size:
2505 1283 494 115 68 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1694 1694
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 228 3326 3510 228
Size: 3098 184 1373
Fragments arranged by size:
3098 1373 184
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 157 625 1094 1405 2644 2771 157
Size: 468 469 311 1239 127 2041
Fragments arranged by size:
2041 1239 469 468 311 127
MspA1I CmG'CkG
Cuts at: 776 1766 2080 2440 3453 3698 776
Size: 990 314 360 1013 245 1733
Fragments arranged by size:
1733 1013 990 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 107
Size: 4655
NdeI CA'TA_TG
Cuts at: 1227 1227
Size: 4655
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NspI r_CATG'y
Cuts at: 945 1245 1708 2208 3115 945
Size: 300 463 500 907 2485
Fragments arranged by size:
2485 907 500 463 300
PciI A'CATG_T
Cuts at: 1241 3111 1241
Size: 1870 2785
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PpuMI rG'GwC_Cy
Cuts at: 204 584 204
Size: 380 4275
PsiI TTA'TAA
Cuts at: 1082 1082
Size: 4655
PstI C_TGCA'G
Cuts at: 579 1702 2387 579
Size: 1123 685 2847
Fragments arranged by size:
2847 1123 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 776 2080 2440 776
Size: 1304 360 2991
Fragments arranged by size:
2991 1304 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1576 1669 1576
Size: 93 4562
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SfcI C'TryA_G
Cuts at: 33 575 1698 2383 3376 3567 33
Size: 542 1123 685 993 191 1121
Fragments arranged by size:
1123 1121 993 685 542 191
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1115 1677 1115
Size: 562 4093
SmlI C'TyrA_G
Cuts at: 1454 1569 1810 2725 3217 3479 1454
Size: 115 241 915 492 262 2630
Fragments arranged by size:
2630 915 492 262 241 115
SpeI A'CTAG_T
Cuts at: 67 1594 67
Size: 1527 3128
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
SspI AAT'ATT
Cuts at: 194 1076 194
Size: 882 3773
StyI C'CwwG_G
Cuts at: 107 1789 2143 107
Size: 1682 354 2619
Fragments arranged by size:
2619 1682 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1480 2238 2371 2830 3039 1480
Size: 758 133 459 209 3096
Fragments arranged by size:
3096 758 459 209 133
TatI w'GTAC_w
Cuts at: 747 1636 4170 747
Size: 889 2534 1232
Fragments arranged by size:
2534 1232 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 4623
Tth111I GACn'n_nGTC
Cuts at: 601 2452 601
Size: 1851 2804
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 4655
XhoI C'TCGA_G
Cuts at: 1454 1454
Size: 4655
XmaI C'CCGG_G
Cuts at: 1113 1675 1113
Size: 562 4093
XmnI GAAnn'nnTTC
Cuts at: 973 973
Size: 4655
ZraI GAC'GTC
Cuts at: 737 737
Size: 4655
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI