Restriction map for
pF1KB9993
(Circular) (Six-base) MAPSORT of: pF1KB9993.seq Check: 1771 from: 1 to: 4865
pF1KB9993 4865 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1199 1833 1903 1199
Size: 634 70 4161
Fragments arranged by size:
4161 634 70
Acc65I G'GTAC_C
Cuts at: 1881 1881
Size: 4865
AclI AA'CG_TT
Cuts at: 4705 4705
Size: 4865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 3122 3869 4137 2690
Size: 432 747 268 3418
Fragments arranged by size:
3418 747 432 268
AflIII A'CryG_T
Cuts at: 2217 3321 4298 2217
Size: 1104 977 2784
Fragments arranged by size:
2784 1104 977
AgeI A'CCGG_T
Cuts at: 3253 3253
Size: 4865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1351 1351
Size: 4865
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1558 1590 1558
Size: 32 4833
AlwNI CAG_nnn'CTG
Cuts at: 1234 2170 3737 1234
Size: 936 1567 2362
Fragments arranged by size:
2362 1567 936
ApaI G_GGCC'C
Cuts at: 239 1445 239
Size: 1206 3659
ApaLI G'TGCA_C
Cuts at: 1469 3635 1469
Size: 2166 2699
ApoI r'AATT_y
Cuts at: 784 1869 4218 4474 784
Size: 1085 2349 256 1175
Fragments arranged by size:
2349 1175 1085 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
AvaI C'yCGr_G
Cuts at: 473 580 1167 1475 1885 473
Size: 107 587 308 410 3453
Fragments arranged by size:
3453 587 410 308 107
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 594 627 594
Size: 33 4832
BamHI G'GATC_C
Cuts at: 906 1890 906
Size: 984 3881
BanI G'GyrC_C
Cuts at: 335 1881 2295 2543 2578 335
Size: 1546 414 248 35 2622
Fragments arranged by size:
2622 1546 414 248 35
BanII G_rGCy'C
Cuts at: 239 1445 1571 1879 2909 239
Size: 1206 126 308 1030 2195
Fragments arranged by size:
2195 1206 1030 308 126
BbeI G_GCGC'C
Cuts at: 339 2299 2547 339
Size: 1960 248 2657
Fragments arranged by size:
2657 1960 248
BbvCI CC'TCA_GC
Cuts at: 1604 1604
Size: 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 1010 976
Size: 34 4831
BciVI GTATCCnnnnn_n'
Cuts at: 2763 3524 4865 2763
Size: 761 1341 2763
Fragments arranged by size:
2763 1341 761
BclI T'GATC_A
Cuts at: 2385 2385
Size: 4865
BglI GCCn_nnn'nGGC
Cuts at: 325 325
Size: 4865
BglII A'GATC_T
Cuts at: 2380 4417 2380
Size: 2037 2828
BlpI GC'TnA_GC
Cuts at: 754 1110 1550 1588 1977 754
Size: 356 440 38 389 3642
Fragments arranged by size:
3642 440 389 356 38
Bme1580I G_kGCm'C
Cuts at: 239 642 1445 1473 2490 2583 3639 239
Size: 403 803 28 1017 93 1056 1465
Fragments arranged by size:
1465 1056 1017 803 403 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 1527 2077 2158 2493 4637 1527
Size: 550 81 335 2144 1755
Fragments arranged by size:
2144 1755 550 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 4865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 415 610 997 1211 190
Size: 225 195 387 214 3844
Fragments arranged by size:
3844 387 225 214 195
Bpu10I CC'TnA_GC
Cuts at: 713 1604 1700 713
Size: 891 96 3878
Fragments arranged by size:
3878 891 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 2920 3412 3710 2041
Size: 879 492 298 3196
Fragments arranged by size:
3196 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4569 4569
Size: 4865
BsaAI yAC'GTr
Cuts at: 2218 2218
Size: 4865
BsaBI GATnn'nnATC
Cuts at: 6 2379 4416 6
Size: 2373 2037 455
Fragments arranged by size:
2373 2037 455
BsaHI Gr'CG_yC
Cuts at: 336 2296 2544 3246 4742 336
Size: 1960 248 702 1496 459
Fragments arranged by size:
1960 1496 702 459 248
BsaWI w'CCGG_w
Cuts at: 146 909 2054 2575 3253 3527 3674 4085 146
Size: 763 1145 521 678 274 147 411 926
Fragments arranged by size:
1145 926 763 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 413 443 1558 1588 413
Size: 30 1115 30 3690
Fragments arranged by size:
3690 1115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 239 418 517 744 786 1138 1141 217
Size: 22 179 99 227 42 352 3 3941
Fragments arranged by size:
3941 352 227 179 99 42 22 3
BseYI C'CCAG_C
Cuts at: 328 1267 1438 2290 3048 3625 4121 328
Size: 939 171 852 758 577 496 1072
Fragments arranged by size:
1072 939 852 758 577 496 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1232 1232
Size: 4865
BsiEI CG_ry'CG
Cuts at: 104 2407 2453 3661 104
Size: 2303 46 1208 1308
Fragments arranged by size:
2303 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 286 1473 1651 1676 1879 2657 2847 3639 286
Size: 1187 178 25 203 778 190 792 1512
Fragments arranged by size:
1512 1187 792 778 203 190 178 25
BsmI GAATG_Cn'
Cuts at: 800 800
Size: 4865
BsmBI CGTCTCn'nnnn_
Cuts at: 819 819
Size: 4865
Bsp1286I G_dGCh'C
Cuts at: 239 286 642 1445 1473 1571 1651 1676 1879
Size: 47 356 803 28 98 80 25 203
Cuts at: 1879 2490 2583 2657 2847 2909 3639 239
Size: 611 93 74 190 62 730 1465
Fragments arranged by size:
1465 803 730 611 356 203 190 98
93 80 74 62 47 28 25
BspEI T'CCGG_A
Cuts at: 146 909 4085 146
Size: 763 3176 926
Fragments arranged by size:
3176 926 763
BspHI T'CATG_A
Cuts at: 1083 4041 1083
Size: 2958 1907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1915 2129 2431 2812 1915
Size: 214 302 381 3968
Fragments arranged by size:
3968 381 302 214
BsrBI CCG'CTC
Cuts at: 50 753 1225 1851 3157 3211 4695 4860 50
Size: 703 472 626 1306 54 1484 165 55
Fragments arranged by size:
1484 1306 703 626 472 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1285 2777 1285
Size: 1492 3373
BsrFI r'CCGG_y
Cuts at: 1592 2863 3044 3253 1592
Size: 1271 181 209 3204
Fragments arranged by size:
3204 1271 209 181
BssSI C'ACGA_G
Cuts at: 3136 3494 3136
Size: 358 4507
BstAPI GCAn_nnn'nTGC
Cuts at: 1255 2242 1255
Size: 987 3878
BstBI TT'CG_AA
Cuts at: 1502 3226 1502
Size: 1724 3141
BstEII G'GTnAC_C
Cuts at: 666 1074 666
Size: 408 4457
BstXI CCAn_nnnn'nTGG
Cuts at: 687 1275 687
Size: 588 4277
BstYI r'GATC_y
Cuts at: 1 111 906 1314 1890 2357 2380 2715 2961
Size: 110 795 408 576 467 23 335 246
Cuts at: 2961 3973 4059 4071 4417 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 795 576 467 449 408 346 335
246 110 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 310 1054 1724 310
Size: 744 670 3451
Fragments arranged by size:
3451 744 670
BtgI C'CryG_G
Cuts at: 107 516 1544 1578 107
Size: 409 1028 34 3394
Fragments arranged by size:
3394 1028 409 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 149 846 2815 2996 3159 149
Size: 697 1969 181 163 1855
Fragments arranged by size:
1969 1855 697 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1433 1473 1609 2108 1244
Size: 189 40 136 499 4001
Fragments arranged by size:
4001 499 189 136 40
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 432 467 432
Size: 35 4830
DraI TTT'AAA
Cuts at: 1864 1864
Size: 4865
DrdI GACnn_nn'nnGTC
Cuts at: 2571 3429 2571
Size: 858 4007
EaeI y'GGCC_r
Cuts at: 317 839 1020 1662 2450 2624 3015 3042 4387
Size: 522 181 642 788 174 391 27 1345
Cuts at: 4387 317
Size: 795
Fragments arranged by size:
1345 795 788 642 522 391 181 174
27
EagI C'GGCC_G
Cuts at: 2450 2450
Size: 4865
EarI CTCTTCn'nnn_
Cuts at: 1127 1517 2888 3098 1127
Size: 390 1371 210 2894
Fragments arranged by size:
2894 1371 390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3073 3383 3529 4675 3073
Size: 310 146 1146 3263
Fragments arranged by size:
3263 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1877 1877
Size: 4865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 415 610 997 1211 2690 3122 3869 4137
Size: 225 195 387 214 1479 432 747 268
Cuts at: 4137 190
Size: 918
Fragments arranged by size:
1479 918 747 432 387 268 225 214
195
EcoNI CCTnn'n_nnAGG
Cuts at: 430 1411 430
Size: 981 3884
EcoO109I rG'GnC_Cy
Cuts at: 235 415 577 621 1442 1479 2004 235
Size: 180 162 44 821 37 525 3096
Fragments arranged by size:
3096 821 525 180 162 44 37
EcoRI G'AATT_C
Cuts at: 1869 1869
Size: 4865
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1528 2884 1528
Size: 1356 3509
FspI TGC'GCA
Cuts at: 2646 2646
Size: 4865
HaeII r_GCGC'y
Cuts at: 339 2299 2547 3569 4548 339
Size: 1960 248 1022 979 656
Fragments arranged by size:
1960 1022 979 656 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 145 177 1326 1358 1369 1401 1731 1763 2707
Size: 32 1149 32 11 32 330 32 944
Cuts at: 2707 2737 2739 2769 145
Size: 30 2 30 2241
Fragments arranged by size:
2241 1149 944 330 32 32 32 32
30 30 11 2
HincII GTy'rAC
Cuts at: 1395 1904 1395
Size: 509 4356
KasI G'GCGC_C
Cuts at: 335 2295 2543 335
Size: 1960 248 2657
Fragments arranged by size:
2657 1960 248
KpnI G_GTAC'C
Cuts at: 1885 1885
Size: 4865
MluI A'CGCG_T
Cuts at: 4298 4298
Size: 4865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3536 3720 3536
Size: 184 4681
MscI TGG'CCA
Cuts at: 319 1022 1664 2626 319
Size: 703 642 962 2558
Fragments arranged by size:
2558 962 703 642
MslI CAynn'nnrTG
Cuts at: 2854 2981 2854
Size: 127 4738
MspA1I CmG'CkG
Cuts at: 370 1976 2290 2650 3663 3908 370
Size: 1606 314 360 1013 245 1327
Fragments arranged by size:
1606 1327 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1594 3046 1594
Size: 1452 3413
NarI GG'CG_CC
Cuts at: 336 2296 2544 336
Size: 1960 248 2657
Fragments arranged by size:
2657 1960 248
NcoI C'CATG_G
Cuts at: 107 1544 1578 107
Size: 1437 34 3394
Fragments arranged by size:
3394 1437 34
NgoMIV G'CCGG_C
Cuts at: 1592 3044 1592
Size: 1452 3413
NspI r_CATG'y
Cuts at: 879 1918 2418 3325 879
Size: 1039 500 907 2419
Fragments arranged by size:
2419 1039 907 500
PasI CC'CwG_GG
Cuts at: 231 340 231
Size: 109 4756
PciI A'CATG_T
Cuts at: 3321 3321
Size: 4865
PflMI CCAn_nnn'nTGG
Cuts at: 975 975
Size: 4865
PfoI T'CCnGG_A
Cuts at: 431 431
Size: 4865
PmeI GTTT'AAAC
Cuts at: 1864 1864
Size: 4865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1558 1590 4004 4036 1558
Size: 32 2414 32 2387
Fragments arranged by size:
2414 2387 32 32
PpuMI rG'GwC_Cy
Cuts at: 577 621 1479 577
Size: 44 858 3963
Fragments arranged by size:
3963 858 44
PshAI GACnn'nnGTC
Cuts at: 266 664 266
Size: 398 4467
PspOMI G'GGCC_C
Cuts at: 235 1441 235
Size: 1206 3659
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1817 1849 1817
Size: 32 4833
PstI C_TGCA'G
Cuts at: 1619 1912 2597 1619
Size: 293 685 3887
Fragments arranged by size:
3887 685 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4865
PvuII CAG'CTG
Cuts at: 370 2290 2650 370
Size: 1920 360 2585
Fragments arranged by size:
2585 1920 360
RsrII CG'GwC_CG
Cuts at: 3060 3060
Size: 4865
SacI G_AGCT'C
Cuts at: 1879 1879
Size: 4865
SalI G'TCGA_C
Cuts at: 1902 1902
Size: 4865
SanDI GG'GwC_CC
Cuts at: 577 577
Size: 4865
SapI GCTCTTCn'nnn_
Cuts at: 1127 1517 2888 3098 1127
Size: 390 1371 210 2894
Fragments arranged by size:
2894 1371 390 210
SbfI CC_TGCA'GG
Cuts at: 1912 1912
Size: 4865
ScaI AGT'ACT
Cuts at: 1153 4382 1153
Size: 3229 1636
SfcI C'TryA_G
Cuts at: 33 741 1292 1615 1834 1908 2593 3586 3777
Size: 708 551 323 219 74 685 993 191
Cuts at: 3777 33
Size: 1121
Fragments arranged by size:
1121 993 708 685 551 323 219 191
74
SfoI GGC'GCC
Cuts at: 337 2297 2545 337
Size: 1960 248 2657
Fragments arranged by size:
2657 1960 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
SmaI CCC'GGG
Cuts at: 582 1169 1477 1887 582
Size: 587 308 410 3560
Fragments arranged by size:
3560 587 410 308
SmlI C'TyrA_G
Cuts at: 2020 2935 3427 3689 2020
Size: 915 492 262 3196
Fragments arranged by size:
3196 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4865
SphI G_CATG'C
Cuts at: 1918 2418 1918
Size: 500 4365
StuI AGG'CCT
Cuts at: 1027 1027
Size: 4865
StyI C'CwwG_G
Cuts at: 107 1184 1544 1578 1999 2353 107
Size: 1077 360 34 421 354 2619
Fragments arranged by size:
2619 1077 421 360 354 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1266 1420 2448 2581 3040 3249 1080
Size: 186 154 1028 133 459 209 2696
Fragments arranged by size:
2696 1028 459 209 186 154 133
TatI w'GTAC_w
Cuts at: 134 1151 4380 134
Size: 1017 3229 619
Fragments arranged by size:
3229 1017 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 4833
Tth111I GACn'n_nGTC
Cuts at: 568 2662 568
Size: 2094 2771
XbaI T'CTAG_A
Cuts at: 1896 1896
Size: 4865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2360 2360
Size: 4865
XmaI C'CCGG_G
Cuts at: 580 1167 1475 1885 580
Size: 587 308 410 3560
Fragments arranged by size:
3560 587 410 308
XmnI GAAnn'nnTTC
Cuts at: 1498 1498
Size: 4865
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BbsI BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI