Restriction map for
pF1KB9983
(Circular) (Six-base) MAPSORT of: pF1KB9983.seq Check: 9424 from: 1 to: 4823
pF1KB9983 4823 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 472 472
Size: 4823
AccI GT'mk_AC
Cuts at: 1861 1861
Size: 4823
Acc65I G'GTAC_C
Cuts at: 952 1839 952
Size: 887 3936
AclI AA'CG_TT
Cuts at: 4663 4663
Size: 4823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 2648 3080 3827 4095 722
Size: 1926 432 747 268 1450
Fragments arranged by size:
1926 1450 747 432 268
AflIII A'CryG_T
Cuts at: 1579 2175 3279 4256 1579
Size: 596 1104 977 2146
Fragments arranged by size:
2146 1104 977 596
AgeI A'CCGG_T
Cuts at: 892 3211 892
Size: 2319 2504
AlwNI CAG_nnn'CTG
Cuts at: 346 464 2128 3695 346
Size: 118 1664 1567 1474
Fragments arranged by size:
1664 1567 1474 118
ApaLI G'TGCA_C
Cuts at: 1618 3593 1618
Size: 1975 2848
ApoI r'AATT_y
Cuts at: 732 1608 1827 4176 4432 732
Size: 876 219 2349 256 1123
Fragments arranged by size:
2349 1123 876 256 219
AseI AT'TA_AT
Cuts at: 20 20
Size: 4823
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
AvaI C'yCGr_G
Cuts at: 572 1843 572
Size: 1271 3552
BamHI G'GATC_C
Cuts at: 1848 1848
Size: 4823
BanI G'GyrC_C
Cuts at: 952 1475 1585 1839 2253 2501 2536 952
Size: 523 110 254 414 248 35 3239
Fragments arranged by size:
3239 523 414 254 248 110 35
BanII G_rGCy'C
Cuts at: 1837 2867 1837
Size: 1030 3793
BbeI G_GCGC'C
Cuts at: 1589 2257 2505 1589
Size: 668 248 3907
Fragments arranged by size:
3907 668 248
BbsI GAAGACnn'nnnn_
Cuts at: 1106 1106
Size: 4823
BciVI GTATCCnnnnn_n'
Cuts at: 1238 2721 3482 4823 1238
Size: 1483 761 1341 1238
Fragments arranged by size:
1483 1341 1238 761
BclI T'GATC_A
Cuts at: 1053 2343 1053
Size: 1290 3533
BfrBI ATG'CAT
Cuts at: 1440 1440
Size: 4823
BglII A'GATC_T
Cuts at: 2338 4375 2338
Size: 2037 2786
BlpI GC'TnA_GC
Cuts at: 1935 1935
Size: 4823
Bme1580I G_kGCm'C
Cuts at: 240 1622 2448 2541 3597 240
Size: 1382 826 93 1056 1466
Fragments arranged by size:
1466 1382 1056 826 93
BmgBI CAC'GTC
Cuts at: 1175 1175
Size: 4823
BmrI ACTGGGnnnn_n'
Cuts at: 2035 2116 2451 4595 2035
Size: 81 335 2144 2263
Fragments arranged by size:
2263 2144 335 81
BmtI G_CTAG'C
Cuts at: 1657 1657
Size: 4823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 605 3190 605
Size: 2585 2238
Bpu10I CC'TnA_GC
Cuts at: 783 783
Size: 4823
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1999 2878 3370 3668 1999
Size: 879 492 298 3154
Fragments arranged by size:
3154 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 883 1711 4527 883
Size: 828 2816 1179
Fragments arranged by size:
2816 1179 828
BsaAI yAC'GTr
Cuts at: 2176 2176
Size: 4823
BsaBI GATnn'nnATC
Cuts at: 6 329 1205 2337 4374 6
Size: 323 876 1132 2037 455
Fragments arranged by size:
2037 1132 876 455 323
BsaHI Gr'CG_yC
Cuts at: 1586 2254 2502 3204 4700 1586
Size: 668 248 702 1496 1709
Fragments arranged by size:
1709 1496 702 668 248
BsaWI w'CCGG_w
Cuts at: 892 1194 1472 1689 2012 2533 3211 3485 3632
Size: 302 278 217 323 521 678 274 147
Cuts at: 3632 4043 892
Size: 411 1672
Fragments arranged by size:
1672 678 521 411 323 302 278 274
217 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1173 1203 1173
Size: 30 4793
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 1012 1181 1641 1755 126
Size: 886 169 460 114 3194
Fragments arranged by size:
3194 886 460 169 114
BseYI C'CCAG_C
Cuts at: 1019 2248 3006 3583 4079 1019
Size: 1229 758 577 496 1763
Fragments arranged by size:
1763 1229 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 627
Size: 4823
BsiEI CG_ry'CG
Cuts at: 104 2365 2411 3619 104
Size: 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 931 1622 1837 2615 2805 3597 931
Size: 691 215 778 190 792 2157
Fragments arranged by size:
2157 792 778 691 215 190
Bsp1286I G_dGCh'C
Cuts at: 240 931 1622 1837 2448 2541 2615 2805 2867
Size: 691 691 215 611 93 74 190 62
Cuts at: 2867 3597 240
Size: 730 1466
Fragments arranged by size:
1466 730 691 691 611 215 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1194 4043 1194
Size: 2849 1974
BspHI T'CATG_A
Cuts at: 476 3999 476
Size: 3523 1300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 472 1324 1873 2087 2389 2770 472
Size: 852 549 214 302 381 2525
Fragments arranged by size:
2525 852 549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4653 4818 50
Size: 3065 54 1484 165 55
Fragments arranged by size:
3065 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1244 2735 1244
Size: 1491 3332
BsrFI r'CCGG_y
Cuts at: 892 2821 3002 3211 892
Size: 1929 181 209 2504
Fragments arranged by size:
2504 1929 209 181
BssSI C'ACGA_G
Cuts at: 3094 3452 3094
Size: 358 4465
BstAPI GCAn_nnn'nTGC
Cuts at: 1359 2200 1359
Size: 841 3982
BstBI TT'CG_AA
Cuts at: 3184 3184
Size: 4823
BstYI r'GATC_y
Cuts at: 1 324 1848 2315 2338 2673 2919 3931 4017
Size: 323 1524 467 23 335 246 1012 86
Cuts at: 4017 4029 4375 1
Size: 12 346 449
Fragments arranged by size:
1524 1012 467 449 346 335 323 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1624 1624
Size: 4823
BtgI C'CryG_G
Cuts at: 107 107
Size: 4823
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1426 2773 2954 3117 1426
Size: 1347 181 163 3132
Fragments arranged by size:
3132 1347 181 163
BtsI GCAGTG_nn'
Cuts at: 270 615 1680 2066 270
Size: 345 1065 386 3027
Fragments arranged by size:
3027 1065 386 345
ClaI AT'CG_AT
Cuts at: 1209 1209
Size: 4823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1563 1598 1563
Size: 35 4788
DraI TTT'AAA
Cuts at: 1822 1822
Size: 4823
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3387 2529
Size: 858 3965
EaeI y'GGCC_r
Cuts at: 2408 2582 2973 3000 4345 2408
Size: 174 391 27 1345 2886
Fragments arranged by size:
2886 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2408 2408
Size: 4823
EarI CTCTTCn'nnn_
Cuts at: 144 783 1709 2846 3056 144
Size: 639 926 1137 210 1911
Fragments arranged by size:
1911 1137 926 639 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3341 3487 4633 3031
Size: 310 146 1146 3221
Fragments arranged by size:
3221 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1835 1835
Size: 4823
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 2648 3080 3827 4095 722
Size: 1926 432 747 268 1450
Fragments arranged by size:
1926 1450 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1092 1962 1092
Size: 870 3953
EcoRI G'AATT_C
Cuts at: 732 1827 732
Size: 1095 3728
EcoRV GAT'ATC
Cuts at: 204 204
Size: 4823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1111 1738 2842 1111
Size: 627 1104 3092
Fragments arranged by size:
3092 1104 627
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4823
HaeII r_GCGC'y
Cuts at: 1589 2257 2505 3527 4506 1589
Size: 668 248 1022 979 1906
Fragments arranged by size:
1906 1022 979 668 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1000 1032 2665 2695 2697 2727 1000
Size: 32 1633 30 2 30 3096
Fragments arranged by size:
3096 1633 32 30 30 2
HincII GTy'rAC
Cuts at: 774 1862 774
Size: 1088 3735
KasI G'GCGC_C
Cuts at: 1585 2253 2501 1585
Size: 668 248 3907
Fragments arranged by size:
3907 668 248
KpnI G_GTAC'C
Cuts at: 956 1843 956
Size: 887 3936
MluI A'CGCG_T
Cuts at: 4256 4256
Size: 4823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 302 1336 3494 3678 302
Size: 1034 2158 184 1447
Fragments arranged by size:
2158 1447 1034 184
MscI TGG'CCA
Cuts at: 2584 2584
Size: 4823
MslI CAynn'nnrTG
Cuts at: 112 197 673 1389 2812 2939 112
Size: 85 476 716 1423 127 1996
Fragments arranged by size:
1996 1423 716 476 127 85
MspA1I CmG'CkG
Cuts at: 1400 1934 2248 2608 3621 3866 1400
Size: 534 314 360 1013 245 2357
Fragments arranged by size:
2357 1013 534 360 314 245
NaeI GCC'GGC
Cuts at: 3004 3004
Size: 4823
NarI GG'CG_CC
Cuts at: 1586 2254 2502 1586
Size: 668 248 3907
Fragments arranged by size:
3907 668 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4823
NgoMIV G'CCGG_C
Cuts at: 3002 3002
Size: 4823
NheI G'CTAG_C
Cuts at: 1653 1653
Size: 4823
NsiI A_TGCA'T
Cuts at: 1442 1442
Size: 4823
NspI r_CATG'y
Cuts at: 1362 1583 1876 2376 3283 1362
Size: 221 293 500 907 2902
Fragments arranged by size:
2902 907 500 293 221
PciI A'CATG_T
Cuts at: 1579 3279 1579
Size: 1700 3123
PfoI T'CCnGG_A
Cuts at: 218 646 1068 218
Size: 428 422 3973
Fragments arranged by size:
3973 428 422
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4791
PpuMI rG'GwC_Cy
Cuts at: 1092 1092
Size: 4823
PstI C_TGCA'G
Cuts at: 1870 2555 1870
Size: 685 4138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4823
PvuII CAG'CTG
Cuts at: 2248 2608 2248
Size: 360 4463
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4823
SacI G_AGCT'C
Cuts at: 1837 1837
Size: 4823
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4823
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4613
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4823
ScaI AGT'ACT
Cuts at: 4340 4340
Size: 4823
SfcI C'TryA_G
Cuts at: 33 1121 1866 2551 3544 3735 33
Size: 1088 745 685 993 191 1121
Fragments arranged by size:
1121 1088 993 745 685 191
SfoI GGC'GCC
Cuts at: 1587 2255 2503 1587
Size: 668 248 3907
Fragments arranged by size:
3907 668 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4823
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4823
SmlI C'TyrA_G
Cuts at: 572 1978 2893 3385 3647 572
Size: 1406 915 492 262 1748
Fragments arranged by size:
1748 1406 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4823
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4323
SspI AAT'ATT
Cuts at: 408 408
Size: 4823
StyI C'CwwG_G
Cuts at: 107 793 1957 2311 107
Size: 686 1164 354 2619
Fragments arranged by size:
2619 1164 686 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 519 2406 2539 2998 3207 100
Size: 419 1887 133 459 209 1716
Fragments arranged by size:
1887 1716 459 419 209 133
TatI w'GTAC_w
Cuts at: 1264 4338 1264
Size: 3074 1749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1173 1205 4340 4372 1173
Size: 32 3135 32 1624
Fragments arranged by size:
3135 1624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1277 2620 1277
Size: 1343 3480
XbaI T'CTAG_A
Cuts at: 528 1854 528
Size: 1326 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 157 2318 157
Size: 2161 2662
XhoI C'TCGA_G
Cuts at: 572 572
Size: 4823
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4823
XmnI GAAnn'nnTTC
Cuts at: 1220 1411 1220
Size: 191 4632
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BpmI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI ZraI