Restriction map for
pF1KB9979
(Circular) (Six-base) MAPSORT of: pF1KB9979.seq Check: 9799 from: 1 to: 4787
pF1KB9979 4787 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1825 1825
Size: 4787
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1231 2612 3044 3791 4059 814
Size: 417 1381 432 747 268 1542
Fragments arranged by size:
1542 1381 747 432 417 268
AflIII A'CryG_T
Cuts at: 2139 3243 4220 2139
Size: 1104 977 2706
Fragments arranged by size:
2706 1104 977
AgeI A'CCGG_T
Cuts at: 3175 3175
Size: 4787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1120 1120
Size: 4787
AlwNI CAG_nnn'CTG
Cuts at: 1209 1506 2092 3659 1209
Size: 297 586 1567 2337
Fragments arranged by size:
2337 1567 586 297
ApaI G_GGCC'C
Cuts at: 1032 1032
Size: 4787
ApaLI G'TGCA_C
Cuts at: 1007 3557 1007
Size: 2550 2237
ApoI r'AATT_y
Cuts at: 371 417 1791 4140 4396 371
Size: 46 1374 2349 256 762
Fragments arranged by size:
2349 1374 762 256 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 637 1187 1748 1807 637
Size: 550 561 59 3617
Fragments arranged by size:
3617 561 550 59
BamHI G'GATC_C
Cuts at: 1812 1812
Size: 4787
BanI G'GyrC_C
Cuts at: 842 1571 1803 2217 2465 2500 842
Size: 729 232 414 248 35 3129
Fragments arranged by size:
3129 729 414 248 232 35
BanII G_rGCy'C
Cuts at: 1032 1551 1602 1757 1801 2831 1032
Size: 519 51 155 44 1030 2988
Fragments arranged by size:
2988 1030 519 155 51 44
BbeI G_GCGC'C
Cuts at: 2221 2469 2221
Size: 248 4539
BbsI GAAGACnn'nnnn_
Cuts at: 205 1253 205
Size: 1048 3739
BciVI GTATCCnnnnn_n'
Cuts at: 440 2685 3446 4787 440
Size: 2245 761 1341 440
Fragments arranged by size:
2245 1341 761 440
BclI T'GATC_A
Cuts at: 1565 2307 1565
Size: 742 4045
BglII A'GATC_T
Cuts at: 126 2302 4339 126
Size: 2176 2037 574
Fragments arranged by size:
2176 2037 574
BlpI GC'TnA_GC
Cuts at: 1899 1899
Size: 4787
Bme1580I G_kGCm'C
Cuts at: 1011 1032 1531 2412 2505 3561 1011
Size: 21 499 881 93 1056 2237
Fragments arranged by size:
2237 1056 881 499 93 21
BmgBI CAC'GTC
Cuts at: 733 733
Size: 4787
BmrI ACTGGGnnnn_n'
Cuts at: 1999 2080 2415 4559 1999
Size: 81 335 2144 2227
Fragments arranged by size:
2227 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 4787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 879 1105 1186 1625 736
Size: 143 226 81 439 3898
Fragments arranged by size:
3898 439 226 143 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 497 938 1102 1160 1760 1963 2842 3334
Size: 399 441 164 58 600 203 879 492
Cuts at: 3334 3632 98
Size: 298 1253
Fragments arranged by size:
1253 879 600 492 441 399 298 203
164 58
BsaI GGTCTCn'nnnn_
Cuts at: 4491 4491
Size: 4787
BsaAI yAC'GTr
Cuts at: 219 2140 219
Size: 1921 2866
BsaBI GATnn'nnATC
Cuts at: 6 542 2301 4338 6
Size: 536 1759 2037 455
Fragments arranged by size:
2037 1759 536 455
BsaHI Gr'CG_yC
Cuts at: 649 2218 2466 3168 4664 649
Size: 1569 248 702 1496 772
Fragments arranged by size:
1569 1496 772 702 248
BsaWI w'CCGG_w
Cuts at: 1976 2497 3175 3449 3596 4007 1976
Size: 521 678 274 147 411 2756
Fragments arranged by size:
2756 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1184 1214 1184
Size: 30 4757
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 1570 706
Size: 864 3923
BseYI C'CCAG_C
Cuts at: 197 850 1455 2212 2970 3547 4043 197
Size: 653 605 757 758 577 496 941
Fragments arranged by size:
941 758 757 653 605 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1538
Size: 4787
BsiEI CG_ry'CG
Cuts at: 104 1619 2329 2375 3583 104
Size: 1515 710 46 1208 1308
Fragments arranged by size:
1515 1308 1208 710 46
BsiHKAI G_wGCw'C
Cuts at: 1011 1428 1551 1757 1801 2579 2769 3561 1011
Size: 417 123 206 44 778 190 792 2237
Fragments arranged by size:
2237 792 778 417 206 190 123 44
BsmI GAATG_Cn'
Cuts at: 931 931
Size: 4787
Bsp1286I G_dGCh'C
Cuts at: 1011 1032 1428 1531 1551 1602 1757 1801 2412
Size: 21 396 103 20 51 155 44 611
Cuts at: 2412 2505 2579 2769 2831 3561 1011
Size: 93 74 190 62 730 2237
Fragments arranged by size:
2237 730 611 396 190 155 103 93
74 62 51 44 21 20
BspEI T'CCGG_A
Cuts at: 4007 4007
Size: 4787
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1514 1837 2051 2353 2734 1514
Size: 323 214 302 381 3567
Fragments arranged by size:
3567 381 323 302 214
BsrBI CCG'CTC
Cuts at: 50 3079 3133 4617 4782 50
Size: 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 872 1478 2699 872
Size: 606 1221 2960
Fragments arranged by size:
2960 1221 606
BsrFI r'CCGG_y
Cuts at: 2785 2966 3175 2785
Size: 181 209 4397
Fragments arranged by size:
4397 209 181
BsrGI T'GTAC_A
Cuts at: 1697 1697
Size: 4787
BssSI C'ACGA_G
Cuts at: 659 1666 3058 3416 659
Size: 1007 1392 358 2030
Fragments arranged by size:
2030 1392 1007 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2164 2164
Size: 4787
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 1258 1258
Size: 4787
BstYI r'GATC_y
Cuts at: 1 126 1812 2279 2302 2637 2883 3895 3981
Size: 125 1686 467 23 335 246 1012 86
Cuts at: 3981 3993 4339 1
Size: 12 346 449
Fragments arranged by size:
1686 1012 467 449 346 335 246 125
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 241 740 241
Size: 499 4288
BtgI C'CryG_G
Cuts at: 107 1154 1364 107
Size: 1047 210 3530
Fragments arranged by size:
3530 1047 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 929 2737 2918 3081 929
Size: 1808 181 163 2635
Fragments arranged by size:
2635 1808 181 163
BtsI GCAGTG_nn'
Cuts at: 1072 1649 2030 1072
Size: 577 381 3829
Fragments arranged by size:
3829 577 381
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 1260 2493 3351 1260
Size: 1233 858 2696
Fragments arranged by size:
2696 1233 858
EaeI y'GGCC_r
Cuts at: 2372 2546 2937 2964 4309 2372
Size: 174 391 27 1345 2850
Fragments arranged by size:
2850 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 2810 3020 2810
Size: 210 4577
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3305 3451 4597 2995
Size: 310 146 1146 3185
Fragments arranged by size:
3185 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1549 1755 1799 1549
Size: 206 44 4537
Fragments arranged by size:
4537 206 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 814 879 1105 1186 1231 1625 2612 3044
Size: 78 65 226 81 45 394 987 432
Cuts at: 3044 3791 4059 736
Size: 747 268 1464
Fragments arranged by size:
1464 987 747 432 394 268 226 81
78 65 45
EcoNI CCTnn'n_nnAGG
Cuts at: 706 706
Size: 4787
EcoO109I rG'GnC_Cy
Cuts at: 1028 1029 1262 1273 1503 1926 1028
Size: 1 233 11 230 423 3889
Fragments arranged by size:
3889 423 233 230 11 1
EcoRI G'AATT_C
Cuts at: 417 1791 417
Size: 1374 3413
EcoRV GAT'ATC
Cuts at: 1182 1182
Size: 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 520 1143 2806 520
Size: 623 1663 2501
Fragments arranged by size:
2501 1663 623
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 2221 2469 3491 4470 2221
Size: 248 1022 979 2538
Fragments arranged by size:
2538 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 894 926 1184 1216 2629 2659 2661
Size: 32 209 32 258 32 1413 30 2
Cuts at: 2661 2691 653
Size: 30 2749
Fragments arranged by size:
2749 1413 258 209 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 313 696 1826 313
Size: 383 1130 3274
Fragments arranged by size:
3274 1130 383
HindIII A'AGCT_T
Cuts at: 160 326 160
Size: 166 4621
KasI G'GCGC_C
Cuts at: 2217 2465 2217
Size: 248 4539
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MfeI C'AATT_G
Cuts at: 305 305
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 313 3458 3642 313
Size: 3145 184 1458
Fragments arranged by size:
3145 1458 184
MscI TGG'CCA
Cuts at: 2548 2548
Size: 4787
MslI CAynn'nnrTG
Cuts at: 605 731 1639 2776 2903 605
Size: 126 908 1137 127 2489
Fragments arranged by size:
2489 1137 908 127 126
MspA1I CmG'CkG
Cuts at: 820 1104 1563 1898 2212 2572 3585 3830 820
Size: 284 459 335 314 360 1013 245 1777
Fragments arranged by size:
1777 1013 459 360 335 314 284 245
NaeI GCC'GGC
Cuts at: 2968 2968
Size: 4787
NarI GG'CG_CC
Cuts at: 2218 2466 2218
Size: 248 4539
NcoI C'CATG_G
Cuts at: 107 1154 107
Size: 1047 3740
NdeI CA'TA_TG
Cuts at: 354 354
Size: 4787
NgoMIV G'CCGG_C
Cuts at: 2966 2966
Size: 4787
NspI r_CATG'y
Cuts at: 1840 2340 3247 1840
Size: 500 907 3380
Fragments arranged by size:
3380 907 500
PciI A'CATG_T
Cuts at: 3243 3243
Size: 4787
PflMI CCAn_nnn'nTGG
Cuts at: 1467 1467
Size: 4787
PfoI T'CCnGG_A
Cuts at: 230 442 1013 1602 230
Size: 212 571 589 3415
Fragments arranged by size:
3415 589 571 212
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PmlI CAC'GTG
Cuts at: 219 219
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1273 1305 3926 3958 1273
Size: 32 2621 32 2102
Fragments arranged by size:
2621 2102 32 32
PpuMI rG'GwC_Cy
Cuts at: 1262 1273 1503 1262
Size: 11 230 4546
Fragments arranged by size:
4546 230 11
PshAI GACnn'nnGTC
Cuts at: 209 1435 209
Size: 1226 3561
PsiI TTA'TAA
Cuts at: 934 934
Size: 4787
PspOMI G'GGCC_C
Cuts at: 1028 1028
Size: 4787
PstI C_TGCA'G
Cuts at: 1511 1834 2519 1511
Size: 323 685 3779
Fragments arranged by size:
3779 685 323
PvuI CG_AT'CG
Cuts at: 104 1619 104
Size: 1515 3272
PvuII CAG'CTG
Cuts at: 820 1104 1563 2212 2572 820
Size: 284 459 649 360 3035
Fragments arranged by size:
3035 649 459 360 284
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 1551 1757 1801 1551
Size: 206 44 4537
Fragments arranged by size:
4537 206 44
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 2810 3020 2810
Size: 210 4577
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 4304 4304
Size: 4787
SfcI C'TryA_G
Cuts at: 33 533 821 1507 1830 2515 3508 3699 33
Size: 500 288 686 323 685 993 191 1121
Fragments arranged by size:
1121 993 686 685 500 323 288 191
SfoI GGC'GCC
Cuts at: 2219 2467 2219
Size: 248 4539
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 113 512 953 1117 1175 1775 1942 2857 3349
Size: 399 441 164 58 600 167 915 492
Cuts at: 3349 3611 113
Size: 262 1289
Fragments arranged by size:
1289 915 600 492 441 399 262 167
164 58
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
StyI C'CwwG_G
Cuts at: 107 1154 1921 2275 107
Size: 1047 767 354 2619
Fragments arranged by size:
2619 1047 767 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2370 2503 2962 3171 2370
Size: 133 459 209 3986
Fragments arranged by size:
3986 459 209 133
TatI w'GTAC_w
Cuts at: 1697 4302 1697
Size: 2605 2182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 438 470 4304 4336 438
Size: 32 3834 32 889
Fragments arranged by size:
3834 889 32 32
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2282 2282
Size: 4787
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstZ17I ClaI CspCI DraIII
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI