Restriction map for
pF1KB9975
(Circular) (Six-base) MAPSORT of: pF1KB9975.seq Check: 9188 from: 1 to: 4706
pF1KB9975 4706 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1679 1679
Size: 4706
AatII G_ACGT'C
Cuts at: 317 317
Size: 4706
AccI GT'mk_AC
Cuts at: 1187 1744 1187
Size: 557 4149
Acc65I G'GTAC_C
Cuts at: 1722 1722
Size: 4706
AclI AA'CG_TT
Cuts at: 4546 4546
Size: 4706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 535 2531 2963 3710 3978 400
Size: 135 1996 432 747 268 1128
Fragments arranged by size:
1996 1128 747 432 268 135
AflIII A'CryG_T
Cuts at: 2058 3162 4139 2058
Size: 1104 977 2625
Fragments arranged by size:
2625 1104 977
AgeI A'CCGG_T
Cuts at: 3094 3094
Size: 4706
AleI CACnn'nnGTG
Cuts at: 436 454 436
Size: 18 4688
AlwNI CAG_nnn'CTG
Cuts at: 780 1383 2011 3578 780
Size: 603 628 1567 1908
Fragments arranged by size:
1908 1567 628 603
ApaI G_GGCC'C
Cuts at: 549 549
Size: 4706
ApaLI G'TGCA_C
Cuts at: 3476 3476
Size: 4706
ApoI r'AATT_y
Cuts at: 1113 1710 4059 4315 1113
Size: 597 2349 256 1504
Fragments arranged by size:
2349 1504 597 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
AvaI C'yCGr_G
Cuts at: 1092 1654 1726 1092
Size: 562 72 4072
Fragments arranged by size:
4072 562 72
BamHI G'GATC_C
Cuts at: 1731 1731
Size: 4706
BanI G'GyrC_C
Cuts at: 1024 1722 2136 2384 2419 1024
Size: 698 414 248 35 3311
Fragments arranged by size:
3311 698 414 248 35
BanII G_rGCy'C
Cuts at: 549 652 1435 1606 1720 2750 549
Size: 103 783 171 114 1030 2505
Fragments arranged by size:
2505 1030 783 171 114 103
BbeI G_GCGC'C
Cuts at: 1028 2140 2388 1028
Size: 1112 248 3346
Fragments arranged by size:
3346 1112 248
BbsI GAAGACnn'nnnn_
Cuts at: 523 523
Size: 4706
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3365 4706 2604
Size: 761 1341 2604
Fragments arranged by size:
2604 1341 761
BclI T'GATC_A
Cuts at: 2226 2226
Size: 4706
BglI GCCn_nnn'nGGC
Cuts at: 553 562 610 1039 1273 553
Size: 9 48 429 234 3986
Fragments arranged by size:
3986 429 234 48 9
BglII A'GATC_T
Cuts at: 2221 4258 2221
Size: 2037 2669
BlpI GC'TnA_GC
Cuts at: 1818 1818
Size: 4706
Bme1580I G_kGCm'C
Cuts at: 549 1298 2331 2424 3480 549
Size: 749 1033 93 1056 1775
Fragments arranged by size:
1775 1056 1033 749 93
BmgBI CAC'GTC
Cuts at: 1197 1197
Size: 4706
BmrI ACTGGGnnnn_n'
Cuts at: 1918 1999 2334 4478 1918
Size: 81 335 2144 2146
Fragments arranged by size:
2146 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 183 3073 183
Size: 2890 1816
Bpu10I CC'TnA_GC
Cuts at: 658 658
Size: 4706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 1882 2761 3253 3551 1501
Size: 381 879 492 298 2656
Fragments arranged by size:
2656 879 492 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 1683 4410 1683
Size: 2727 1979
BsaAI yAC'GTr
Cuts at: 2059 2059
Size: 4706
BsaBI GATnn'nnATC
Cuts at: 6 292 1087 2220 4257 6
Size: 286 795 1133 2037 455
Fragments arranged by size:
2037 1133 795 455 286
BsaHI Gr'CG_yC
Cuts at: 272 314 1025 1184 1544 2137 2385 3087 4583
Size: 42 711 159 360 593 248 702 1496
Cuts at: 4583 272
Size: 395
Fragments arranged by size:
1496 711 702 593 395 360 248 159
42
BsaWI w'CCGG_w
Cuts at: 296 1895 2416 3094 3368 3515 3926 296
Size: 1599 521 678 274 147 411 1076
Fragments arranged by size:
1599 1076 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 1522 1627 433
Size: 1089 105 3512
Fragments arranged by size:
3512 1089 105
BseYI C'CCAG_C
Cuts at: 809 1268 2131 2889 3466 3962 809
Size: 459 863 758 577 496 1553
Fragments arranged by size:
1553 863 758 577 496 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 691 843 1219 1264 1288 1369 1396 1459
Size: 404 152 376 45 24 81 27 63
Cuts at: 1459 1624 287
Size: 165 3369
Fragments arranged by size:
3369 404 376 165 152 81 63 45
27 24
BsiEI CG_ry'CG
Cuts at: 104 617 2248 2294 3502 104
Size: 513 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 513 46
BsiHKAI G_wGCw'C
Cuts at: 659 1720 2498 2688 3480 659
Size: 1061 778 190 792 1885
Fragments arranged by size:
1885 1061 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 549 652 659 1298 1435 1606 1720 2331 2424
Size: 103 7 639 137 171 114 611 93
Cuts at: 2424 2498 2688 2750 3480 549
Size: 74 190 62 730 1775
Fragments arranged by size:
1775 730 639 611 190 171 137 114
103 93 74 62 7
BspEI T'CCGG_A
Cuts at: 296 3926 296
Size: 3630 1076
BspHI T'CATG_A
Cuts at: 197 3882 197
Size: 3685 1021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 953 1066 1646 1679 1756 1970 2272 2653 953
Size: 113 580 33 77 214 302 381 3006
Fragments arranged by size:
3006 580 381 302 214 113 77 33
BsrBI CCG'CTC
Cuts at: 50 2998 3052 4536 4701 50
Size: 2948 54 1484 165 55
Fragments arranged by size:
2948 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1542 2618 1542
Size: 1076 3630
BsrFI r'CCGG_y
Cuts at: 2704 2885 3094 2704
Size: 181 209 4316
Fragments arranged by size:
4316 209 181
BssSI C'ACGA_G
Cuts at: 2977 3335 2977
Size: 358 4348
BstAPI GCAn_nnn'nTGC
Cuts at: 1302 2083 1302
Size: 781 3925
BstBI TT'CG_AA
Cuts at: 3067 3067
Size: 4706
BstEII G'GTnAC_C
Cuts at: 360 456 360
Size: 96 4610
BstXI CCAn_nnnn'nTGG
Cuts at: 435 435
Size: 4706
BstYI r'GATC_y
Cuts at: 1 151 1211 1512 1731 2198 2221 2556 2802
Size: 150 1060 301 219 467 23 335 246
Cuts at: 2802 3814 3900 3912 4258 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1060 1012 467 449 346 335 301 246
219 150 86 23 12
BtgI C'CryG_G
Cuts at: 107 434 617 983 989 1020 1607 107
Size: 327 183 366 6 31 587 3206
Fragments arranged by size:
3206 587 366 327 183 31 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 649 2656 2837 3000 649
Size: 2007 181 163 2355
Fragments arranged by size:
2355 2007 181 163
BtsI GCAGTG_nn'
Cuts at: 1949 1949
Size: 4706
DraI TTT'AAA
Cuts at: 1705 1705
Size: 4706
DrdI GACnn_nn'nnGTC
Cuts at: 118 2412 3270 118
Size: 2294 858 1554
Fragments arranged by size:
2294 1554 858
EaeI y'GGCC_r
Cuts at: 474 614 638 986 1040 1274 1466 2291 2465
Size: 140 24 348 54 234 192 825 174
Cuts at: 2465 2856 2883 4228 474
Size: 391 27 1345 952
Fragments arranged by size:
1345 952 825 391 348 234 192 174
140 54 27 24
EagI C'GGCC_G
Cuts at: 614 2291 614
Size: 1677 3029
EarI CTCTTCn'nnn_
Cuts at: 685 2729 2939 685
Size: 2044 210 2452
Fragments arranged by size:
2452 2044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1267 2914 3224 3370 4516 1267
Size: 1647 310 146 1146 1457
Fragments arranged by size:
1647 1457 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1718 1718
Size: 4706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 535 2531 2963 3710 3978 400
Size: 135 1996 432 747 268 1128
Fragments arranged by size:
1996 1128 747 432 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 1387 1387
Size: 4706
EcoO109I rG'GnC_Cy
Cuts at: 777 1380 1521 1626 1845 777
Size: 603 141 105 219 3638
Fragments arranged by size:
3638 603 219 141 105
EcoRI G'AATT_C
Cuts at: 1710 1710
Size: 4706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4706
FspI TGC'GCA
Cuts at: 868 2487 868
Size: 1619 3087
HaeII r_GCGC'y
Cuts at: 809 1028 2140 2388 3410 4389 809
Size: 219 1112 248 1022 979 1126
Fragments arranged by size:
1126 1112 1022 979 248 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 179 211 2548 2578 2580 2610 179
Size: 32 2337 30 2 30 2275
Fragments arranged by size:
2337 2275 32 30 30 2
HincII GTy'rAC
Cuts at: 1745 1745
Size: 4706
KasI G'GCGC_C
Cuts at: 1024 2136 2384 1024
Size: 1112 248 3346
Fragments arranged by size:
3346 1112 248
KpnI G_GTAC'C
Cuts at: 1726 1726
Size: 4706
MluI A'CGCG_T
Cuts at: 4139 4139
Size: 4706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 1060 1508 3377 3561 524
Size: 536 448 1869 184 1669
Fragments arranged by size:
1869 1669 536 448 184
MscI TGG'CCA
Cuts at: 1042 1276 2467 1042
Size: 234 1191 3281
Fragments arranged by size:
3281 1191 234
MslI CAynn'nnrTG
Cuts at: 406 436 454 2695 2822 406
Size: 30 18 2241 127 2290
Fragments arranged by size:
2290 2241 127 30 18
MspA1I CmG'CkG
Cuts at: 991 1066 1272 1353 1817 2131 2491 3504 3749
Size: 75 206 81 464 314 360 1013 245
Cuts at: 3749 991
Size: 1948
Fragments arranged by size:
1948 1013 464 360 314 245 206 81
75
NaeI GCC'GGC
Cuts at: 2887 2887
Size: 4706
NarI GG'CG_CC
Cuts at: 1025 2137 2385 1025
Size: 1112 248 3346
Fragments arranged by size:
3346 1112 248
NcoI C'CATG_G
Cuts at: 107 434 983 107
Size: 327 549 3830
Fragments arranged by size:
3830 549 327
NgoMIV G'CCGG_C
Cuts at: 2885 2885
Size: 4706
NspI r_CATG'y
Cuts at: 1759 2259 3166 1759
Size: 500 907 3299
Fragments arranged by size:
3299 907 500
PasI CC'CwG_GG
Cuts at: 339 339
Size: 4706
PciI A'CATG_T
Cuts at: 3162 3162
Size: 4706
PflMI CCAn_nnn'nTGG
Cuts at: 984 984
Size: 4706
PmeI GTTT'AAAC
Cuts at: 1705 1705
Size: 4706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 4674
PpuMI rG'GwC_Cy
Cuts at: 1521 1521
Size: 4706
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 4706
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 667 699 667
Size: 32 4674
PstI C_TGCA'G
Cuts at: 222 563 788 1753 2438 222
Size: 341 225 965 685 2490
Fragments arranged by size:
2490 965 685 341 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4706
PvuII CAG'CTG
Cuts at: 1066 1272 1353 2131 2491 1066
Size: 206 81 778 360 3281
Fragments arranged by size:
3281 778 360 206 81
RsrII CG'GwC_CG
Cuts at: 187 2901 187
Size: 2714 1992
SacI G_AGCT'C
Cuts at: 1720 1720
Size: 4706
SacII CC_GC'GG
Cuts at: 992 992
Size: 4706
SalI G'TCGA_C
Cuts at: 1743 1743
Size: 4706
SapI GCTCTTCn'nnn_
Cuts at: 2729 2939 2729
Size: 210 4496
SbfI CC_TGCA'GG
Cuts at: 563 1753 563
Size: 1190 3516
ScaI AGT'ACT
Cuts at: 4223 4223
Size: 4706
SfcI C'TryA_G
Cuts at: 33 218 559 784 1749 2434 3427 3618 33
Size: 185 341 225 965 685 993 191 1121
Fragments arranged by size:
1121 993 965 685 341 225 191 185
SfiI GGCCn_nnn'nGGCC
Cuts at: 553 1039 1273 553
Size: 486 234 3986
Fragments arranged by size:
3986 486 234
SfoI GGC'GCC
Cuts at: 1026 2138 2386 1026
Size: 1112 248 3346
Fragments arranged by size:
3346 1112 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
SmaI CCC'GGG
Cuts at: 1094 1728 1094
Size: 634 4072
SmlI C'TyrA_G
Cuts at: 1516 1861 2776 3268 3530 1516
Size: 345 915 492 262 2692
Fragments arranged by size:
2692 915 492 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4706
SphI G_CATG'C
Cuts at: 1759 2259 1759
Size: 500 4206
StuI AGG'CCT
Cuts at: 397 397
Size: 4706
StyI C'CwwG_G
Cuts at: 107 434 596 983 1388 1840 2194 107
Size: 327 162 387 405 452 354 2619
Fragments arranged by size:
2619 452 405 387 354 327 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 535 564 2289 2422 2881 3090 535
Size: 29 1725 133 459 209 2151
Fragments arranged by size:
2151 1725 459 209 133 29
TatI w'GTAC_w
Cuts at: 905 4221 905
Size: 3316 1390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4223 4255 4223
Size: 32 4674
Tth111I GACn'n_nGTC
Cuts at: 2503 2503
Size: 4706
XbaI T'CTAG_A
Cuts at: 280 1737 280
Size: 1457 3249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 825 1095 2201 825
Size: 270 1106 3330
Fragments arranged by size:
3330 1106 270
XmaI C'CCGG_G
Cuts at: 1092 1726 1092
Size: 634 4072
XmnI GAAnn'nnTTC
Cuts at: 679 1453 679
Size: 774 3932
ZraI GAC'GTC
Cuts at: 315 315
Size: 4706
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BpmI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PshAI PsiI
PspXI SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI