Restriction map for
pF1KB9970
(Circular) (Six-base) MAPSORT of: pF1KB9970.seq Check: 4734 from: 1 to: 5222
pF1KB9970 5222 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1203 1203
Size: 5222
AccI GT'mk_AC
Cuts at: 1478 2260 1478
Size: 782 4440
Acc65I G'GTAC_C
Cuts at: 2238 2238
Size: 5222
AclI AA'CG_TT
Cuts at: 5062 5062
Size: 5222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2149 3047 3479 4226 4494 1964
Size: 185 898 432 747 268 2692
Fragments arranged by size:
2692 898 747 432 268 185
AfeI AGC'GCT
Cuts at: 1321 1321
Size: 5222
AflIII A'CryG_T
Cuts at: 1103 2574 3678 4655 1103
Size: 1471 1104 977 1670
Fragments arranged by size:
1670 1471 1104 977
AgeI A'CCGG_T
Cuts at: 3610 3610
Size: 5222
AleI CACnn'nnGTG
Cuts at: 421 1870 421
Size: 1449 3773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 5222
AlwNI CAG_nnn'CTG
Cuts at: 729 848 1454 2527 4094 729
Size: 119 606 1073 1567 1857
Fragments arranged by size:
1857 1567 1073 606 119
ApaI G_GGCC'C
Cuts at: 662 2113 662
Size: 1451 3771
ApaLI G'TGCA_C
Cuts at: 1885 3992 1885
Size: 2107 3115
ApoI r'AATT_y
Cuts at: 2226 4575 4831 2226
Size: 2349 256 2617
Fragments arranged by size:
2617 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5222
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
AvaI C'yCGr_G
Cuts at: 1327 2242 1327
Size: 915 4307
BamHI G'GATC_C
Cuts at: 958 2247 958
Size: 1289 3933
BanI G'GyrC_C
Cuts at: 1331 2238 2652 2900 2935 1331
Size: 907 414 248 35 3618
Fragments arranged by size:
3618 907 414 248 35
BanII G_rGCy'C
Cuts at: 662 823 869 1099 1205 1369 2113 2236 3266
Size: 161 46 230 106 164 744 123 1030
Cuts at: 3266 662
Size: 2618
Fragments arranged by size:
2618 1030 744 230 164 161 123 106
46
BbeI G_GCGC'C
Cuts at: 1335 2656 2904 1335
Size: 1321 248 3653
Fragments arranged by size:
3653 1321 248
BbsI GAAGACnn'nnnn_
Cuts at: 516 1433 1553 1825 516
Size: 917 120 272 3913
Fragments arranged by size:
3913 917 272 120
BciVI GTATCCnnnnn_n'
Cuts at: 3120 3881 5222 3120
Size: 761 1341 3120
Fragments arranged by size:
3120 1341 761
BclI T'GATC_A
Cuts at: 2742 2742
Size: 5222
BfrBI ATG'CAT
Cuts at: 767 1082 767
Size: 315 4907
BglI GCCn_nnn'nGGC
Cuts at: 653 1340 1508 1993 653
Size: 687 168 485 3882
Fragments arranged by size:
3882 687 485 168
BglII A'GATC_T
Cuts at: 2737 4774 2737
Size: 2037 3185
BlpI GC'TnA_GC
Cuts at: 1199 1519 2334 1199
Size: 320 815 4087
Fragments arranged by size:
4087 815 320
Bme1580I G_kGCm'C
Cuts at: 662 1059 1066 1124 1777 1889 2113 2847 2940
Size: 397 7 58 653 112 224 734 93
Cuts at: 2940 3996 662
Size: 1056 1888
Fragments arranged by size:
1888 1056 734 653 397 224 112 93
58 7
BmrI ACTGGGnnnn_n'
Cuts at: 2434 2515 2850 4994 2434
Size: 81 335 2144 2662
Fragments arranged by size:
2662 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3589 3589
Size: 5222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 346 430 1792 2155 226
Size: 120 84 1362 363 3293
Fragments arranged by size:
3293 1362 363 120 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2398 3277 3769 4067 2398
Size: 879 492 298 3553
Fragments arranged by size:
3553 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 547 1720 2169 4926 547
Size: 1173 449 2757 843
Fragments arranged by size:
2757 1173 843 449
BsaAI yAC'GTr
Cuts at: 1106 2575 1106
Size: 1469 3753
BsaBI GATnn'nnATC
Cuts at: 6 2736 4773 6
Size: 2730 2037 455
Fragments arranged by size:
2730 2037 455
BsaHI Gr'CG_yC
Cuts at: 1332 2653 2901 3603 5099 1332
Size: 1321 248 702 1496 1455
Fragments arranged by size:
1496 1455 1321 702 248
BsaWI w'CCGG_w
Cuts at: 230 556 2411 2932 3610 3884 4031 4442 230
Size: 326 1855 521 678 274 147 411 1010
Fragments arranged by size:
1855 1010 678 521 411 326 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1573 1603 1699 1729 1777 1807 1879 1909 1573
Size: 30 96 30 48 30 72 30 4886
Fragments arranged by size:
4886 96 72 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 625 665 1168 343
Size: 282 40 503 4397
Fragments arranged by size:
4397 503 282 40
BseYI C'CCAG_C
Cuts at: 450 1362 2647 3405 3982 4478 450
Size: 912 1285 758 577 496 1194
Fragments arranged by size:
1285 1194 912 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1461 1647 319
Size: 1142 186 3894
Fragments arranged by size:
3894 1142 186
BsiEI CG_ry'CG
Cuts at: 104 182 2764 2810 4018 104
Size: 78 2582 46 1208 1308
Fragments arranged by size:
2582 1308 1208 78 46
BsiHKAI G_wGCw'C
Cuts at: 869 1889 2236 3014 3204 3996 869
Size: 1020 347 778 190 792 2095
Fragments arranged by size:
2095 1020 792 778 347 190
BsmI GAATG_Cn'
Cuts at: 292 769 1261 292
Size: 477 492 4253
Fragments arranged by size:
4253 492 477
BsmBI CGTCTCn'nnnn_
Cuts at: 2205 2205
Size: 5222
Bsp1286I G_dGCh'C
Cuts at: 662 823 869 1059 1066 1099 1124 1205 1369
Size: 161 46 190 7 33 25 81 164
Cuts at: 1369 1777 1889 2113 2236 2847 2940 3014 3204
Size: 408 112 224 123 611 93 74 190
Cuts at: 3204 3266 3996 662
Size: 62 730 1888
Fragments arranged by size:
1888 730 611 408 224 190 190 164
161 123 112 93 81 74 62 46
33 25 7
BspEI T'CCGG_A
Cuts at: 4442 4442
Size: 5222
BspHI T'CATG_A
Cuts at: 4398 4398
Size: 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1203 1406 1738 2036 2272 2486 2788 3169 1203
Size: 203 332 298 236 214 302 381 3256
Fragments arranged by size:
3256 381 332 302 298 236 214 203
BsrBI CCG'CTC
Cuts at: 50 1793 3514 3568 5052 5217 50
Size: 1743 1721 54 1484 165 55
Fragments arranged by size:
1743 1721 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 438 3134 438
Size: 2696 2526
BsrFI r'CCGG_y
Cuts at: 788 1502 3220 3401 3610 788
Size: 714 1718 181 209 2400
Fragments arranged by size:
2400 1718 714 209 181
BssHII G'CGCG_C
Cuts at: 114 1187 114
Size: 1073 4149
BssSI C'ACGA_G
Cuts at: 3493 3851 3493
Size: 358 4864
BstAPI GCAn_nnn'nTGC
Cuts at: 2599 2599
Size: 5222
BstBI TT'CG_AA
Cuts at: 3583 3583
Size: 5222
BstYI r'GATC_y
Cuts at: 1 958 2247 2714 2737 3072 3318 4330 4416
Size: 957 1289 467 23 335 246 1012 86
Cuts at: 4416 4428 4774 1
Size: 12 346 449
Fragments arranged by size:
1289 1012 957 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 344 390 1134 1388 2075 107
Size: 237 46 744 254 687 3254
Fragments arranged by size:
3254 744 687 254 237 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1204 1538 3172 3353 3516 1204
Size: 334 1634 181 163 2910
Fragments arranged by size:
2910 1634 334 181 163
BtsI GCAGTG_nn'
Cuts at: 313 679 2465 313
Size: 366 1786 3070
Fragments arranged by size:
3070 1786 366
DraI TTT'AAA
Cuts at: 2221 2221
Size: 5222
DrdI GACnn_nn'nnGTC
Cuts at: 196 697 2928 3786 196
Size: 501 2231 858 1632
Fragments arranged by size:
2231 1632 858 501
EaeI y'GGCC_r
Cuts at: 179 236 359 1131 1341 1665 2807 2981 3372
Size: 57 123 772 210 324 1142 174 391
Cuts at: 3372 3399 4744 179
Size: 27 1345 657
Fragments arranged by size:
1345 1142 772 657 391 324 210 174
123 57 27
EagI C'GGCC_G
Cuts at: 179 2807 179
Size: 2628 2594
EarI CTCTTCn'nnn_
Cuts at: 421 1940 3245 3455 421
Size: 1519 1305 210 2188
Fragments arranged by size:
2188 1519 1305 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3430 3740 3886 5032 3430
Size: 310 146 1146 3620
Fragments arranged by size:
3620 1146 310 146
EcoICRI GAG'CTC
Cuts at: 867 2234 867
Size: 1367 3855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 226 346 430 1792 1964 2149 2155 3047 3479
Size: 120 84 1362 172 185 6 892 432
Cuts at: 3479 4226 4494 226
Size: 747 268 954
Fragments arranged by size:
1362 954 892 747 432 268 185 172
120 84 6
EcoNI CCTnn'n_nnAGG
Cuts at: 205 205
Size: 5222
EcoO109I rG'GnC_Cy
Cuts at: 135 576 624 659 2361 135
Size: 441 48 35 1702 2996
Fragments arranged by size:
2996 1702 441 48 35
EcoRI G'AATT_C
Cuts at: 2226 2226
Size: 5222
EcoRV GAT'ATC
Cuts at: 1032 1032
Size: 5222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1014 3241 1014
Size: 2227 2995
FspI TGC'GCA
Cuts at: 3003 3003
Size: 5222
HaeII r_GCGC'y
Cuts at: 1323 1335 2656 2904 3926 4905 1323
Size: 12 1321 248 1022 979 1640
Fragments arranged by size:
1640 1321 1022 979 248 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 225 257 727 759 1402 1434 3064 3094 3096
Size: 32 470 32 643 32 1630 30 2
Cuts at: 3096 3126 225
Size: 30 2321
Fragments arranged by size:
2321 1630 643 470 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1308 2184 2261 1308
Size: 876 77 4269
Fragments arranged by size:
4269 876 77
HindIII A'AGCT_T
Cuts at: 1617 1617
Size: 5222
KasI G'GCGC_C
Cuts at: 1331 2652 2900 1331
Size: 1321 248 3653
Fragments arranged by size:
3653 1321 248
KpnI G_GTAC'C
Cuts at: 2242 2242
Size: 5222
MluI A'CGCG_T
Cuts at: 4655 4655
Size: 5222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3893 4077 3893
Size: 184 5038
MscI TGG'CCA
Cuts at: 238 361 1343 2983 238
Size: 123 982 1640 2477
Fragments arranged by size:
2477 1640 982 123
MslI CAynn'nnrTG
Cuts at: 421 1870 2074 2174 3211 3338 421
Size: 1449 204 100 1037 127 2305
Fragments arranged by size:
2305 1449 1037 204 127 100
MspA1I CmG'CkG
Cuts at: 147 324 377 644 726 785 830 837 848
Size: 177 53 267 82 59 45 7 11
Cuts at: 848 968 1250 1382 1598 1631 1757 1835 2333
Size: 120 282 132 216 33 126 78 498
Cuts at: 2333 2647 3007 4020 4265 147
Size: 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 498 360 314 282 267 245
216 177 132 126 120 82 78 59
53 45 33 11 7
NaeI GCC'GGC
Cuts at: 790 1504 3403 790
Size: 714 1899 2609
Fragments arranged by size:
2609 1899 714
NarI GG'CG_CC
Cuts at: 1332 2653 2901 1332
Size: 1321 248 3653
Fragments arranged by size:
3653 1321 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5222
NdeI CA'TA_TG
Cuts at: 267 267
Size: 5222
NgoMIV G'CCGG_C
Cuts at: 788 1502 3401 788
Size: 714 1899 2609
Fragments arranged by size:
2609 1899 714
NsiI A_TGCA'T
Cuts at: 769 1084 769
Size: 315 4907
NspI r_CATG'y
Cuts at: 536 1082 1871 2275 2775 3682 536
Size: 546 789 404 500 907 2076
Fragments arranged by size:
2076 907 789 546 500 404
PasI CC'CwG_GG
Cuts at: 471 471
Size: 5222
PciI A'CATG_T
Cuts at: 3678 3678
Size: 5222
PfoI T'CCnGG_A
Cuts at: 203 203
Size: 5222
PmeI GTTT'AAAC
Cuts at: 2221 2221
Size: 5222
PmlI CAC'GTG
Cuts at: 1106 1106
Size: 5222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2229 4361 4393 2197
Size: 32 2132 32 3026
Fragments arranged by size:
3026 2132 32 32
PpuMI rG'GwC_Cy
Cuts at: 135 624 135
Size: 489 4733
PspOMI G'GGCC_C
Cuts at: 658 2109 658
Size: 1451 3771
PstI C_TGCA'G
Cuts at: 1381 2269 2954 1381
Size: 888 685 3649
Fragments arranged by size:
3649 888 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5222
PvuII CAG'CTG
Cuts at: 324 726 785 848 1382 1631 2647 3007 324
Size: 402 59 63 534 249 1016 360 2539
Fragments arranged by size:
2539 1016 534 402 360 249 63 59
RsrII CG'GwC_CG
Cuts at: 1830 3417 1830
Size: 1587 3635
SacI G_AGCT'C
Cuts at: 869 2236 869
Size: 1367 3855
SalI G'TCGA_C
Cuts at: 2259 2259
Size: 5222
SanDI GG'GwC_CC
Cuts at: 135 135
Size: 5222
SapI GCTCTTCn'nnn_
Cuts at: 3245 3455 3245
Size: 210 5012
SbfI CC_TGCA'GG
Cuts at: 2269 2269
Size: 5222
ScaI AGT'ACT
Cuts at: 2209 4739 2209
Size: 2530 2692
SfcI C'TryA_G
Cuts at: 33 982 1377 1455 1647 2265 2950 3943 4134
Size: 949 395 78 192 618 685 993 191
Cuts at: 4134 33
Size: 1121
Fragments arranged by size:
1121 993 949 685 618 395 192 191
78
SfoI GGC'GCC
Cuts at: 1333 2654 2902 1333
Size: 1321 248 3653
Fragments arranged by size:
3653 1321 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5222
SmaI CCC'GGG
Cuts at: 1329 2244 1329
Size: 915 4307
SmlI C'TyrA_G
Cuts at: 2377 3292 3784 4046 2377
Size: 915 492 262 3553
Fragments arranged by size:
3553 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5222
SphI G_CATG'C
Cuts at: 536 2275 2775 536
Size: 1739 500 2983
Fragments arranged by size:
2983 1739 500
StuI AGG'CCT
Cuts at: 211 211
Size: 5222
StyI C'CwwG_G
Cuts at: 107 2356 2710 107
Size: 2249 354 2619
Fragments arranged by size:
2619 2249 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2068 2805 2938 3397 3606 2068
Size: 737 133 459 209 3684
Fragments arranged by size:
3684 737 459 209 133
TatI w'GTAC_w
Cuts at: 488 2207 4737 488
Size: 1719 2530 973
Fragments arranged by size:
2530 1719 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1573 1605 1699 1731 1879 1911 4739 4771 1573
Size: 32 94 32 148 32 2828 32 2024
Fragments arranged by size:
2828 2024 148 94 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1828 3019 1828
Size: 1191 4031
XbaI T'CTAG_A
Cuts at: 2253 2253
Size: 5222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2717 2717
Size: 5222
XmaI C'CCGG_G
Cuts at: 1327 2242 1327
Size: 915 4307
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BcgI BmgBI BmtI Bpu10I BsiWI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NheI NotI NruI PacI PflMI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI