Restriction map for
pF1KB9967
(Circular) (Six-base) MAPSORT of: pF1KB9967.seq Check: 7243 from: 1 to: 4766
pF1KB9967 4766 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 476 476
Size: 4766
AccI GT'mk_AC
Cuts at: 476 1804 476
Size: 1328 3438
Acc65I G'GTAC_C
Cuts at: 1359 1782 1359
Size: 423 4343
AclI AA'CG_TT
Cuts at: 620 4606 620
Size: 3986 780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1007 1093 2591 3023 3770 4038 639
Size: 368 86 1498 432 747 268 1367
Fragments arranged by size:
1498 1367 747 432 368 268 86
AflIII A'CryG_T
Cuts at: 2118 3222 4199 2118
Size: 1104 977 2685
Fragments arranged by size:
2685 1104 977
AgeI A'CCGG_T
Cuts at: 3154 3154
Size: 4766
AleI CACnn'nnGTG
Cuts at: 490 490
Size: 4766
AlwNI CAG_nnn'CTG
Cuts at: 290 872 2071 3638 290
Size: 582 1199 1567 1418
Fragments arranged by size:
1567 1418 1199 582
ApaI G_GGCC'C
Cuts at: 155 155
Size: 4766
ApaLI G'TGCA_C
Cuts at: 813 3536 813
Size: 2723 2043
ApoI r'AATT_y
Cuts at: 767 1250 1408 1770 4119 4375 767
Size: 483 158 362 2349 256 1158
Fragments arranged by size:
2349 1158 483 362 256 158
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 391 1786 391
Size: 1395 3371
BamHI G'GATC_C
Cuts at: 1791 1791
Size: 4766
BanI G'GyrC_C
Cuts at: 336 531 900 1359 1782 2196 2444 2479 336
Size: 195 369 459 423 414 248 35 2623
Fragments arranged by size:
2623 459 423 414 369 248 195 35
BanII G_rGCy'C
Cuts at: 155 1460 1780 2810 155
Size: 1305 320 1030 2111
Fragments arranged by size:
2111 1305 1030 320
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 1437 1437
Size: 4766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 238 272 90
Size: 34 114 34 4584
Fragments arranged by size:
4584 114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3425 4766 2664
Size: 761 1341 2664
Fragments arranged by size:
2664 1341 761
BclI T'GATC_A
Cuts at: 2286 2286
Size: 4766
BglI GCCn_nnn'nGGC
Cuts at: 452 452
Size: 4766
BglII A'GATC_T
Cuts at: 728 1693 2281 4318 728
Size: 965 588 2037 1176
Fragments arranged by size:
2037 1176 965 588
BlpI GC'TnA_GC
Cuts at: 181 1454 1878 181
Size: 1273 424 3069
Fragments arranged by size:
3069 1273 424
Bme1580I G_kGCm'C
Cuts at: 155 341 536 817 2391 2484 3540 155
Size: 186 195 281 1574 93 1056 1381
Fragments arranged by size:
1574 1381 1056 281 195 186 93
BmgBI CAC'GTC
Cuts at: 414 414
Size: 4766
BmrI ACTGGGnnnn_n'
Cuts at: 1978 2059 2394 4538 1978
Size: 81 335 2144 2206
Fragments arranged by size:
2206 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 451 1494 418
Size: 33 1043 3690
Fragments arranged by size:
3690 1043 33
Bpu10I CC'TnA_GC
Cuts at: 280 280
Size: 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1942 2821 3313 3611 1942
Size: 879 492 298 3097
Fragments arranged by size:
3097 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4470 4470
Size: 4766
BsaAI yAC'GTr
Cuts at: 2119 2119
Size: 4766
BsaBI GATnn'nnATC
Cuts at: 6 1609 2280 4317 6
Size: 1603 671 2037 455
Fragments arranged by size:
2037 1603 671 455
BsaHI Gr'CG_yC
Cuts at: 269 473 2197 2445 3147 4643 269
Size: 204 1724 248 702 1496 392
Fragments arranged by size:
1724 1496 702 392 248 204
BsaWI w'CCGG_w
Cuts at: 1955 2476 3154 3428 3575 3986 1955
Size: 521 678 274 147 411 2735
Fragments arranged by size:
2735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1322 1352 1322
Size: 30 4736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 559 1072 1346 559
Size: 513 274 3979
Fragments arranged by size:
3979 513 274
BseYI C'CCAG_C
Cuts at: 455 683 1401 2191 2949 3526 4022 455
Size: 228 718 790 758 577 496 1199
Fragments arranged by size:
1199 790 758 718 577 496 228
BsiEI CG_ry'CG
Cuts at: 104 330 2308 2354 3562 104
Size: 226 1978 46 1208 1308
Fragments arranged by size:
1978 1308 1208 226 46
BsiHKAI G_wGCw'C
Cuts at: 251 569 817 1460 1780 2558 2748 3540 251
Size: 318 248 643 320 778 190 792 1477
Fragments arranged by size:
1477 792 778 643 320 318 248 190
BsmI GAATG_Cn'
Cuts at: 1571 1756 1571
Size: 185 4581
Bsp1286I G_dGCh'C
Cuts at: 155 251 341 536 569 817 1460 1780 2391
Size: 96 90 195 33 248 643 320 611
Cuts at: 2391 2484 2558 2748 2810 3540 155
Size: 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 643 611 320 248 195 190
96 93 90 74 62 33
BspEI T'CCGG_A
Cuts at: 3986 3986
Size: 4766
BspHI T'CATG_A
Cuts at: 3942 3942
Size: 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1816 2030 2332 2713 1816
Size: 214 302 381 3869
Fragments arranged by size:
3869 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1526 2678 1526
Size: 1152 3614
BsrFI r'CCGG_y
Cuts at: 359 2764 2945 3154 359
Size: 2405 181 209 1971
Fragments arranged by size:
2405 1971 209 181
BsrGI T'GTAC_A
Cuts at: 642 642
Size: 4766
BssSI C'ACGA_G
Cuts at: 3037 3395 3037
Size: 358 4408
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstXI CCAn_nnnn'nTGG
Cuts at: 741 1392 741
Size: 651 4115
BstYI r'GATC_y
Cuts at: 1 728 1693 1791 2258 2281 2616 2862 3874
Size: 727 965 98 467 23 335 246 1012
Cuts at: 3874 3960 3972 4318 1
Size: 86 12 346 449
Fragments arranged by size:
1012 965 727 467 449 346 335 246
98 86 23 12
BtgI C'CryG_G
Cuts at: 635 1502 635
Size: 867 3899
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 357 2716 2897 3060 357
Size: 2359 181 163 2063
Fragments arranged by size:
2359 2063 181 163
BtsI GCAGTG_nn'
Cuts at: 1043 1448 2009 1043
Size: 405 561 3800
Fragments arranged by size:
3800 561 405
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 1137 2472 3330 1137
Size: 1335 858 2573
Fragments arranged by size:
2573 1335 858
EaeI y'GGCC_r
Cuts at: 327 2351 2525 2916 2943 4288 327
Size: 2024 174 391 27 1345 805
Fragments arranged by size:
2024 1345 805 391 174 27
EagI C'GGCC_G
Cuts at: 327 2351 327
Size: 2024 2742
EarI CTCTTCn'nnn_
Cuts at: 1351 1655 2789 2999 1351
Size: 304 1134 210 3118
Fragments arranged by size:
3118 1134 304 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 891 2974 3284 3430 4576 891
Size: 2083 310 146 1146 1081
Fragments arranged by size:
2083 1146 1081 310 146
EcoICRI GAG'CTC
Cuts at: 1458 1778 1458
Size: 320 4446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 451 639 1007 1093 1494 2591 3023 3770
Size: 33 188 368 86 401 1097 432 747
Cuts at: 3770 4038 418
Size: 268 1146
Fragments arranged by size:
1146 1097 747 432 401 368 268 188
86 33
EcoO109I rG'GnC_Cy
Cuts at: 151 152 239 1905 151
Size: 1 87 1666 3012
Fragments arranged by size:
3012 1666 87 1
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
EcoRV GAT'ATC
Cuts at: 962 993 962
Size: 31 4735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4766
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 165 188 290 391 895 2200 2448 3470 4449
Size: 23 102 101 504 1305 248 1022 979
Cuts at: 4449 165
Size: 482
Fragments arranged by size:
1305 1022 979 504 482 248 102 101
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1322 1354 2608 2638 2640 2670 1322
Size: 32 1254 30 2 30 3418
Fragments arranged by size:
3418 1254 32 30 30 2
HincII GTy'rAC
Cuts at: 1805 1805
Size: 4766
HindIII A'AGCT_T
Cuts at: 1085 1085
Size: 4766
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1363 1786 1363
Size: 423 4343
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 758 3437 3621 758
Size: 2679 184 1903
Fragments arranged by size:
2679 1903 184
MscI TGG'CCA
Cuts at: 2527 2527
Size: 4766
MslI CAynn'nnrTG
Cuts at: 490 1211 1486 1582 2755 2882 490
Size: 721 275 96 1173 127 2374
Fragments arranged by size:
2374 1173 721 275 127 96
MspA1I CmG'CkG
Cuts at: 254 312 347 429 594 683 924 1877 2191
Size: 58 35 82 165 89 241 953 314
Cuts at: 2191 2551 3564 3809 254
Size: 360 1013 245 1211
Fragments arranged by size:
1211 1013 953 360 314 245 241 165
89 82 58 35
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 635 635
Size: 4766
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NspI r_CATG'y
Cuts at: 1819 2319 3226 1819
Size: 500 907 3359
Fragments arranged by size:
3359 907 500
PciI A'CATG_T
Cuts at: 3222 3222
Size: 4766
PflMI CCAn_nnn'nTGG
Cuts at: 683 683
Size: 4766
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4734
PpuMI rG'GwC_Cy
Cuts at: 239 239
Size: 4766
PspOMI G'GGCC_C
Cuts at: 151 151
Size: 4766
PstI C_TGCA'G
Cuts at: 352 668 1317 1813 2498 352
Size: 316 649 496 685 2620
Fragments arranged by size:
2620 685 649 496 316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 347 429 594 683 2191 2551 347
Size: 82 165 89 1508 360 2562
Fragments arranged by size:
2562 1508 360 165 89 82
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 1460 1780 1460
Size: 320 4446
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SanDI GG'GwC_CC
Cuts at: 239 239
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 2789 2999 2789
Size: 210 4556
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 1319 4283 1319
Size: 2964 1802
SfcI C'TryA_G
Cuts at: 33 348 664 1313 1809 2494 3487 3678 33
Size: 315 316 649 496 685 993 191 1121
Fragments arranged by size:
1121 993 685 649 496 316 315 191
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 1921 2836 3328 3590 1921
Size: 915 492 262 3097
Fragments arranged by size:
3097 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
SspI AAT'ATT
Cuts at: 1119 1119
Size: 4766
StyI C'CwwG_G
Cuts at: 635 1739 1900 2254 635
Size: 1104 161 354 3147
Fragments arranged by size:
3147 1104 354 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 2349 2482 2941 3150 511
Size: 1838 133 459 209 2127
Fragments arranged by size:
2127 1838 459 209 133
TatI w'GTAC_w
Cuts at: 642 1317 4281 642
Size: 675 2964 1127
Fragments arranged by size:
2964 1127 675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 4734
Tth111I GACn'n_nGTC
Cuts at: 2563 2563
Size: 4766
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2261 2261
Size: 4766
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
XmnI GAAnn'nnTTC
Cuts at: 1087 1228 1687 1087
Size: 141 459 4166
Fragments arranged by size:
4166 459 141
ZraI GAC'GTC
Cuts at: 474 474
Size: 4766
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BsgI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI