Restriction map for
pF1KB9965
(Circular) (Six-base) MAPSORT of: pF1KB9965.seq Check: 8101 from: 1 to: 4760
pF1KB9965 4760 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 406 1798 406
Size: 1392 3368
Acc65I G'GTAC_C
Cuts at: 307 1776 307
Size: 1469 3291
AclI AA'CG_TT
Cuts at: 4600 4600
Size: 4760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 913 1544 2585 3017 3764 4032 503
Size: 410 631 1041 432 747 268 1231
Fragments arranged by size:
1231 1041 747 631 432 410 268
AfeI AGC'GCT
Cuts at: 1243 1243
Size: 4760
AflIII A'CryG_T
Cuts at: 2112 3216 4193 2112
Size: 1104 977 2679
Fragments arranged by size:
2679 1104 977
AgeI A'CCGG_T
Cuts at: 3148 3148
Size: 4760
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1262 1412 1262
Size: 150 4610
AlwNI CAG_nnn'CTG
Cuts at: 2065 3632 2065
Size: 1567 3193
ApaLI G'TGCA_C
Cuts at: 1247 3530 1247
Size: 2283 2477
ApoI r'AATT_y
Cuts at: 502 559 896 1365 1513 1764 4113 4369 502
Size: 57 337 469 148 251 2349 256 893
Fragments arranged by size:
2349 893 469 337 256 251 148 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 4760
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
AvaI C'yCGr_G
Cuts at: 1780 1780
Size: 4760
BamHI G'GATC_C
Cuts at: 1785 1785
Size: 4760
BanI G'GyrC_C
Cuts at: 187 307 1776 2190 2438 2473 187
Size: 120 1469 414 248 35 2474
Fragments arranged by size:
2474 1469 414 248 120 35
BanII G_rGCy'C
Cuts at: 1774 2804 1774
Size: 1030 3730
BbeI G_GCGC'C
Cuts at: 2194 2442 2194
Size: 248 4512
BbsI GAAGACnn'nnnn_
Cuts at: 1471 1570 1471
Size: 99 4661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1312 1346 1312
Size: 34 4726
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3419 4760 2658
Size: 761 1341 2658
Fragments arranged by size:
2658 1341 761
BclI T'GATC_A
Cuts at: 2280 2280
Size: 4760
BglI GCCn_nnn'nGGC
Cuts at: 203 203
Size: 4760
BglII A'GATC_T
Cuts at: 2275 4312 2275
Size: 2037 2723
BlpI GC'TnA_GC
Cuts at: 541 833 1872 541
Size: 292 1039 3429
Fragments arranged by size:
3429 1039 292
Bme1580I G_kGCm'C
Cuts at: 190 602 1251 2385 2478 3534 190
Size: 412 649 1134 93 1056 1416
Fragments arranged by size:
1416 1134 1056 649 412 93
BmrI ACTGGGnnnn_n'
Cuts at: 313 658 1493 1972 2053 2388 4532 313
Size: 345 835 479 81 335 2144 541
Fragments arranged by size:
2144 835 541 479 345 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3127 3127
Size: 4760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4760
Bpu10I CC'TnA_GC
Cuts at: 494 494
Size: 4760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1936 2815 3307 3605 1936
Size: 879 492 298 3091
Fragments arranged by size:
3091 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4464 4464
Size: 4760
BsaAI yAC'GTr
Cuts at: 2113 2113
Size: 4760
BsaBI GATnn'nnATC
Cuts at: 6 2274 4311 6
Size: 2268 2037 455
Fragments arranged by size:
2268 2037 455
BsaHI Gr'CG_yC
Cuts at: 275 2191 2439 3141 4637 275
Size: 1916 248 702 1496 398
Fragments arranged by size:
1916 1496 702 398 248
BsaWI w'CCGG_w
Cuts at: 1949 2470 3148 3422 3569 3980 1949
Size: 521 678 274 147 411 2729
Fragments arranged by size:
2729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 235
Size: 30 4730
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 259 756 247
Size: 12 497 4251
Fragments arranged by size:
4251 497 12
BseYI C'CCAG_C
Cuts at: 2185 2943 3520 4016 2185
Size: 758 577 496 2929
Fragments arranged by size:
2929 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 142
Size: 4760
BsiEI CG_ry'CG
Cuts at: 104 2302 2348 3556 104
Size: 2198 46 1208 1308
Fragments arranged by size:
2198 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1251 1774 2552 2742 3534 1251
Size: 523 778 190 792 2477
Fragments arranged by size:
2477 792 778 523 190
BsmI GAATG_Cn'
Cuts at: 376 480 376
Size: 104 4656
Bsp1286I G_dGCh'C
Cuts at: 190 602 1251 1774 2385 2478 2552 2742 2804
Size: 412 649 523 611 93 74 190 62
Cuts at: 2804 3534 190
Size: 730 1416
Fragments arranged by size:
1416 730 649 611 523 412 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3980 3980
Size: 4760
BspHI T'CATG_A
Cuts at: 3936 3936
Size: 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1810 2024 2326 2707 1810
Size: 214 302 381 3863
Fragments arranged by size:
3863 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3052 3106 4590 4755 50
Size: 3002 54 1484 165 55
Fragments arranged by size:
3002 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2672 2672
Size: 4760
BsrFI r'CCGG_y
Cuts at: 161 2758 2939 3148 161
Size: 2597 181 209 1773
Fragments arranged by size:
2597 1773 209 181
BssSI C'ACGA_G
Cuts at: 3031 3389 3031
Size: 358 4402
BstAPI GCAn_nnn'nTGC
Cuts at: 194 2137 194
Size: 1943 2817
BstBI TT'CG_AA
Cuts at: 336 1704 3121 336
Size: 1368 1417 1975
Fragments arranged by size:
1975 1417 1368
BstEII G'GTnAC_C
Cuts at: 1134 1225 1134
Size: 91 4669
BstYI r'GATC_y
Cuts at: 1 1325 1615 1785 2252 2275 2610 2856 3868
Size: 1324 290 170 467 23 335 246 1012
Cuts at: 3868 3954 3966 4312 1
Size: 86 12 346 449
Fragments arranged by size:
1324 1012 467 449 346 335 290 246
170 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 781 781
Size: 4760
BtgI C'CryG_G
Cuts at: 107 136 107
Size: 29 4731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2710 2891 3054 2710
Size: 181 163 4416
Fragments arranged by size:
4416 181 163
BtsI GCAGTG_nn'
Cuts at: 2003 2003
Size: 4760
ClaI AT'CG_AT
Cuts at: 611 611
Size: 4760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 837 872 837
Size: 35 4725
DraI TTT'AAA
Cuts at: 1759 1759
Size: 4760
DraIII CAC_nnn'GTG
Cuts at: 194 194
Size: 4760
DrdI GACnn_nn'nnGTC
Cuts at: 2466 3324 2466
Size: 858 3902
EaeI y'GGCC_r
Cuts at: 2345 2519 2910 2937 4282 2345
Size: 174 391 27 1345 2823
Fragments arranged by size:
2823 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2345 2345
Size: 4760
EarI CTCTTCn'nnn_
Cuts at: 629 1309 2783 2993 629
Size: 680 1474 210 2396
Fragments arranged by size:
2396 1474 680 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2968 3278 3424 4570 2968
Size: 310 146 1146 3158
Fragments arranged by size:
3158 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1772 1772
Size: 4760
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 913 1114 1544 2585 3017 3764 4032 503
Size: 410 201 430 1041 432 747 268 1231
Fragments arranged by size:
1231 1041 747 432 430 410 268 201
EcoO109I rG'GnC_Cy
Cuts at: 1194 1899 1194
Size: 705 4055
EcoRI G'AATT_C
Cuts at: 896 1764 896
Size: 868 3892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4760
FspI TGC'GCA
Cuts at: 2541 2541
Size: 4760
HaeII r_GCGC'y
Cuts at: 660 1245 2194 2442 3464 4443 660
Size: 585 949 248 1022 979 977
Fragments arranged by size:
1022 979 977 949 585 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 802 834 1317 1349 2602 2632 2634
Size: 32 382 32 483 32 1253 30 2
Cuts at: 2634 2664 388
Size: 30 2484
Fragments arranged by size:
2484 1253 483 382 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1656 1799 1656
Size: 143 4617
HindIII A'AGCT_T
Cuts at: 956 956
Size: 4760
HpaI GTT'AAC
Cuts at: 1656 1656
Size: 4760
KasI G'GCGC_C
Cuts at: 2190 2438 2190
Size: 248 4512
KpnI G_GTAC'C
Cuts at: 311 1780 311
Size: 1469 3291
MfeI C'AATT_G
Cuts at: 587 587
Size: 4760
MluI A'CGCG_T
Cuts at: 4193 4193
Size: 4760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 329 1588 3431 3615 329
Size: 1259 1843 184 1474
Fragments arranged by size:
1843 1474 1259 184
MscI TGG'CCA
Cuts at: 2521 2521
Size: 4760
MslI CAynn'nnrTG
Cuts at: 427 1394 2749 2876 427
Size: 967 1355 127 2311
Fragments arranged by size:
2311 1355 967 127
MspA1I CmG'CkG
Cuts at: 138 1871 2185 2545 3558 3803 138
Size: 1733 314 360 1013 245 1095
Fragments arranged by size:
1733 1095 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2941 2941
Size: 4760
NarI GG'CG_CC
Cuts at: 2191 2439 2191
Size: 248 4512
NcoI C'CATG_G
Cuts at: 107 107
Size: 4760
NdeI CA'TA_TG
Cuts at: 1641 1641
Size: 4760
NgoMIV G'CCGG_C
Cuts at: 2939 2939
Size: 4760
NspI r_CATG'y
Cuts at: 1393 1813 2313 3220 1393
Size: 420 500 907 2933
Fragments arranged by size:
2933 907 500 420
PciI A'CATG_T
Cuts at: 3216 3216
Size: 4760
PflMI CCAn_nnn'nTGG
Cuts at: 533 727 533
Size: 194 4566
PfoI T'CCnGG_A
Cuts at: 357 357
Size: 4760
PmeI GTTT'AAAC
Cuts at: 1759 1759
Size: 4760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 3899
Size: 32 4728
PstI C_TGCA'G
Cuts at: 1220 1807 2492 1220
Size: 587 685 3488
Fragments arranged by size:
3488 685 587
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4760
PvuII CAG'CTG
Cuts at: 2185 2545 2185
Size: 360 4400
RsrII CG'GwC_CG
Cuts at: 2955 2955
Size: 4760
SacI G_AGCT'C
Cuts at: 1774 1774
Size: 4760
SacII CC_GC'GG
Cuts at: 139 139
Size: 4760
SalI G'TCGA_C
Cuts at: 1797 1797
Size: 4760
SapI GCTCTTCn'nnn_
Cuts at: 629 2783 2993 629
Size: 2154 210 2396
Fragments arranged by size:
2396 2154 210
SbfI CC_TGCA'GG
Cuts at: 1807 1807
Size: 4760
ScaI AGT'ACT
Cuts at: 668 4277 668
Size: 3609 1151
SfcI C'TryA_G
Cuts at: 33 1216 1456 1803 2488 3481 3672 33
Size: 1183 240 347 685 993 191 1121
Fragments arranged by size:
1183 1121 993 685 347 240 191
SfoI GGC'GCC
Cuts at: 2192 2440 2192
Size: 248 4512
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4760
SmaI CCC'GGG
Cuts at: 1782 1782
Size: 4760
SmlI C'TyrA_G
Cuts at: 1915 2830 3322 3584 1915
Size: 915 492 262 3091
Fragments arranged by size:
3091 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4760
SphI G_CATG'C
Cuts at: 1393 1813 2313 1393
Size: 420 500 3840
Fragments arranged by size:
3840 500 420
SspI AAT'ATT
Cuts at: 1094 1094
Size: 4760
StuI AGG'CCT
Cuts at: 1009 1009
Size: 4760
StyI C'CwwG_G
Cuts at: 107 1142 1894 2248 107
Size: 1035 752 354 2619
Fragments arranged by size:
2619 1035 752 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1001 1244 2343 2476 2935 3144 1001
Size: 243 1099 133 459 209 2617
Fragments arranged by size:
2617 1099 459 243 209 133
TatI w'GTAC_w
Cuts at: 666 4275 666
Size: 3609 1151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 1167 1199 1303 1335 4277 4309 235
Size: 32 900 32 104 32 2942 32 686
Fragments arranged by size:
2942 900 686 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1470 2557 1470
Size: 1087 3673
XbaI T'CTAG_A
Cuts at: 1791 1791
Size: 4760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 688 2255 688
Size: 1567 3193
XmaI C'CCGG_G
Cuts at: 1780 1780
Size: 4760
XmnI GAAnn'nnTTC
Cuts at: 623 1119 623
Size: 496 4264
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstXI BstZ17I EcoNI EcoRV FseI FspAI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI