Restriction map for
pF1KB9962
(Circular) (Six-base) MAPSORT of: pF1KB9962.seq Check: 2748 from: 1 to: 5189
pF1KB9962 5189 bp
With 182 enzymes: SgfI *
September 25, 2012 15:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1550 1550
Size: 5189
AccI GT'mk_AC
Cuts at: 2227 2227
Size: 5189
Acc65I G'GTAC_C
Cuts at: 2205 2205
Size: 5189
AclI AA'CG_TT
Cuts at: 5029 5029
Size: 5189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1252 3014 3446 4193 4461 501
Size: 751 1762 432 747 268 1229
Fragments arranged by size:
1762 1229 751 747 432 268
AflIII A'CryG_T
Cuts at: 806 2541 3645 4622 806
Size: 1735 1104 977 1373
Fragments arranged by size:
1735 1373 1104 977
AgeI A'CCGG_T
Cuts at: 3577 3577
Size: 5189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 592 624 770 802 592
Size: 32 146 32 4979
Fragments arranged by size:
4979 146 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2083 2494 4061 2083
Size: 411 1567 3211
Fragments arranged by size:
3211 1567 411
ApaI G_GGCC'C
Cuts at: 186 186
Size: 5189
ApaLI G'TGCA_C
Cuts at: 3959 3959
Size: 5189
ApoI r'AATT_y
Cuts at: 632 944 1713 2193 4542 4798 632
Size: 312 769 480 2349 256 1023
Fragments arranged by size:
2349 1023 769 480 312 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5189
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5189
AvaI C'yCGr_G
Cuts at: 1999 2209 1999
Size: 210 4979
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1352 1385 1352
Size: 33 5156
BamHI G'GATC_C
Cuts at: 2214 2214
Size: 5189
BanI G'GyrC_C
Cuts at: 2205 2619 2867 2902 2205
Size: 414 248 35 4492
Fragments arranged by size:
4492 414 248 35
BanII G_rGCy'C
Cuts at: 186 240 325 700 1438 1531 2050 2203 3233
Size: 54 85 375 738 93 519 153 1030
Cuts at: 3233 186
Size: 2142
Fragments arranged by size:
2142 1030 738 519 375 153 93 85
54
BbeI G_GCGC'C
Cuts at: 2623 2871 2623
Size: 248 4941
BbsI GAAGACnn'nnnn_
Cuts at: 1181 1181
Size: 5189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1420 1454 1420
Size: 34 5155
BciVI GTATCCnnnnn_n'
Cuts at: 149 594 1899 3087 3848 5189 149
Size: 445 1305 1188 761 1341 149
Fragments arranged by size:
1341 1305 1188 761 445 149
BclI T'GATC_A
Cuts at: 2709 2709
Size: 5189
BfrBI ATG'CAT
Cuts at: 871 871
Size: 5189
BglII A'GATC_T
Cuts at: 1521 2704 4741 1521
Size: 1183 2037 1969
Fragments arranged by size:
2037 1969 1183
BlpI GC'TnA_GC
Cuts at: 2301 2301
Size: 5189
Bme1580I G_kGCm'C
Cuts at: 186 2814 2907 3963 186
Size: 2628 93 1056 1412
Fragments arranged by size:
2628 1412 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1231 1563 1716 2401 2482 2817 4961 1231
Size: 332 153 685 81 335 2144 1459
Fragments arranged by size:
2144 1459 685 335 332 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3556 3556
Size: 5189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1982 2060 1049
Size: 933 78 4178
Fragments arranged by size:
4178 933 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1113 2365 3244 3736 4034 1113
Size: 1252 879 492 298 2268
Fragments arranged by size:
2268 1252 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4893 4893
Size: 5189
BsaAI yAC'GTr
Cuts at: 2542 2542
Size: 5189
BsaBI GATnn'nnATC
Cuts at: 6 1277 2703 4740 6
Size: 1271 1426 2037 455
Fragments arranged by size:
2037 1426 1271 455
BsaHI Gr'CG_yC
Cuts at: 2620 2868 3570 5066 2620
Size: 248 702 1496 2743
Fragments arranged by size:
2743 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2378 2899 3577 3851 3998 4409 2378
Size: 521 678 274 147 411 3158
Fragments arranged by size:
3158 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 770 800 1195 1225 1690 1720 298
Size: 30 442 30 395 30 465 30 3767
Fragments arranged by size:
3767 465 442 395 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 661 1056 1279 1695 661
Size: 395 223 416 4155
Fragments arranged by size:
4155 416 395 223
BseYI C'CCAG_C
Cuts at: 1503 1757 2614 3372 3949 4445 1503
Size: 254 857 758 577 496 2247
Fragments arranged by size:
2247 857 758 577 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 541 285
Size: 256 4933
BsiEI CG_ry'CG
Cuts at: 104 2731 2777 3985 104
Size: 2627 46 1208 1308
Fragments arranged by size:
2627 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1438 2050 2203 2981 3171 3963 1438
Size: 612 153 778 190 792 2664
Fragments arranged by size:
2664 792 778 612 190 153
Bsp1286I G_dGCh'C
Cuts at: 186 240 325 700 1438 1531 2050 2203 2814
Size: 54 85 375 738 93 519 153 611
Cuts at: 2814 2907 2981 3171 3233 3963 186
Size: 93 74 190 62 730 1412
Fragments arranged by size:
1412 738 730 611 519 375 190 153
93 93 85 74 62 54
BspEI T'CCGG_A
Cuts at: 4409 4409
Size: 5189
BspHI T'CATG_A
Cuts at: 4365 4365
Size: 5189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1550 2239 2453 2755 3136 1550
Size: 689 214 302 381 3603
Fragments arranged by size:
3603 689 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3481 3535 5019 5184 50
Size: 3431 54 1484 165 55
Fragments arranged by size:
3431 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3101 3101
Size: 5189
BsrFI r'CCGG_y
Cuts at: 875 1184 3187 3368 3577 875
Size: 309 2003 181 209 2487
Fragments arranged by size:
2487 2003 309 209 181
BsrGI T'GTAC_A
Cuts at: 503 503
Size: 5189
BssSI C'ACGA_G
Cuts at: 3460 3818 3460
Size: 358 4831
BstAPI GCAn_nnn'nTGC
Cuts at: 2566 2566
Size: 5189
BstBI TT'CG_AA
Cuts at: 3550 3550
Size: 5189
BstYI r'GATC_y
Cuts at: 1 827 863 1521 1651 2214 2681 2704 3039
Size: 826 36 658 130 563 467 23 335
Cuts at: 3039 3285 4297 4383 4395 4741 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 826 658 563 467 449 346 335
246 130 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 178 1346 178
Size: 1168 4021
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3139 3320 3483 3139
Size: 181 163 4845
Fragments arranged by size:
4845 181 163
BtsI GCAGTG_nn'
Cuts at: 204 1510 1989 2432 204
Size: 1306 479 443 2961
Fragments arranged by size:
2961 1306 479 443
DraI TTT'AAA
Cuts at: 1587 2188 1587
Size: 601 4588
DraIII CAC_nnn'GTG
Cuts at: 983 983
Size: 5189
DrdI GACnn_nn'nnGTC
Cuts at: 2895 3753 2895
Size: 858 4331
EaeI y'GGCC_r
Cuts at: 131 2774 2948 3339 3366 4711 131
Size: 2643 174 391 27 1345 609
Fragments arranged by size:
2643 1345 609 391 174 27
EagI C'GGCC_G
Cuts at: 2774 2774
Size: 5189
EarI CTCTTCn'nnn_
Cuts at: 413 1228 2055 3212 3422 413
Size: 815 827 1157 210 2180
Fragments arranged by size:
2180 1157 827 815 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3397 3707 3853 4999 3397
Size: 310 146 1146 3587
Fragments arranged by size:
3587 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1436 2048 2201 1436
Size: 612 153 4424
Fragments arranged by size:
4424 612 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 1049 1252 1982 2060 3014 3446 4193 4461
Size: 548 203 730 78 954 432 747 268
Cuts at: 4461 501
Size: 1229
Fragments arranged by size:
1229 954 747 730 548 432 268 203
78
EcoNI CCTnn'n_nnAGG
Cuts at: 1546 1546
Size: 5189
EcoO109I rG'GnC_Cy
Cuts at: 182 2328 182
Size: 2146 3043
EcoRI G'AATT_C
Cuts at: 944 1713 2193 944
Size: 769 480 3940
Fragments arranged by size:
3940 769 480
EcoRV GAT'ATC
Cuts at: 386 386
Size: 5189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3208 3208
Size: 5189
FspI TGC'GCA
Cuts at: 2970 2970
Size: 5189
HaeII r_GCGC'y
Cuts at: 2623 2871 3893 4872 2623
Size: 248 1022 979 2940
Fragments arranged by size:
2940 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 819 851 1513 1545 1675 1707 1753
Size: 32 399 32 662 32 130 32 46
Cuts at: 1753 1785 1835 1867 1897 1929 3031 3061 3063
Size: 32 50 32 30 32 1102 30 2
Cuts at: 3063 3093 388
Size: 30 2484
Fragments arranged by size:
2484 1102 662 399 130 50 46 32
32 32 32 32 32 32 30 30
30 2
HincII GTy'rAC
Cuts at: 317 1727 2228 317
Size: 1410 501 3278
Fragments arranged by size:
3278 1410 501
HindIII A'AGCT_T
Cuts at: 1666 1666
Size: 5189
HpaI GTT'AAC
Cuts at: 317 317
Size: 5189
KasI G'GCGC_C
Cuts at: 2619 2867 2619
Size: 248 4941
KpnI G_GTAC'C
Cuts at: 2209 2209
Size: 5189
MluI A'CGCG_T
Cuts at: 4622 4622
Size: 5189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 166 239 1496 3860 4044 166
Size: 73 1257 2364 184 1311
Fragments arranged by size:
2364 1311 1257 184 73
MscI TGG'CCA
Cuts at: 2950 2950
Size: 5189
MslI CAynn'nnrTG
Cuts at: 1147 3178 3305 1147
Size: 2031 127 3031
Fragments arranged by size:
3031 2031 127
MspA1I CmG'CkG
Cuts at: 445 2300 2614 2974 3987 4232 445
Size: 1855 314 360 1013 245 1402
Fragments arranged by size:
1855 1402 1013 360 314 245
NaeI GCC'GGC
Cuts at: 877 3370 877
Size: 2493 2696
NarI GG'CG_CC
Cuts at: 2620 2868 2620
Size: 248 4941
NgoMIV G'CCGG_C
Cuts at: 875 3368 875
Size: 2493 2696
NsiI A_TGCA'T
Cuts at: 873 873
Size: 5189
NspI r_CATG'y
Cuts at: 810 822 875 2242 2742 3649 810
Size: 12 53 1367 500 907 2350
Fragments arranged by size:
2350 1367 907 500 53 12
PciI A'CATG_T
Cuts at: 806 3645 806
Size: 2839 2350
PflMI CCAn_nnn'nTGG
Cuts at: 983 983
Size: 5189
PfoI T'CCnGG_A
Cuts at: 456 456
Size: 5189
PmeI GTTT'AAAC
Cuts at: 2188 2188
Size: 5189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 770 802 4328 4360 770
Size: 32 3526 32 1599
Fragments arranged by size:
3526 1599 32 32
PspOMI G'GGCC_C
Cuts at: 182 182
Size: 5189
PstI C_TGCA'G
Cuts at: 1368 1417 2236 2921 1368
Size: 49 819 685 3636
Fragments arranged by size:
3636 819 685 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5189
PvuII CAG'CTG
Cuts at: 445 2614 2974 445
Size: 2169 360 2660
Fragments arranged by size:
2660 2169 360
RsrII CG'GwC_CG
Cuts at: 3384 3384
Size: 5189
SacI G_AGCT'C
Cuts at: 1438 2050 2203 1438
Size: 612 153 4424
Fragments arranged by size:
4424 612 153
SalI G'TCGA_C
Cuts at: 2226 2226
Size: 5189
SapI GCTCTTCn'nnn_
Cuts at: 2055 3212 3422 2055
Size: 1157 210 3822
Fragments arranged by size:
3822 1157 210
SbfI CC_TGCA'GG
Cuts at: 2236 2236
Size: 5189
ScaI AGT'ACT
Cuts at: 1959 4706 1959
Size: 2747 2442
SfcI C'TryA_G
Cuts at: 33 1071 1364 1413 1839 2232 2917 3910 4101
Size: 1038 293 49 426 393 685 993 191
Cuts at: 4101 33
Size: 1121
Fragments arranged by size:
1121 1038 993 685 426 393 293 191
49
SfoI GGC'GCC
Cuts at: 2621 2869 2621
Size: 248 4941
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5189
SmaI CCC'GGG
Cuts at: 2211 2211
Size: 5189
SmlI C'TyrA_G
Cuts at: 1092 2344 3259 3751 4013 1092
Size: 1252 915 492 262 2268
Fragments arranged by size:
2268 1252 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5189
SphI G_CATG'C
Cuts at: 875 2242 2742 875
Size: 1367 500 3322
Fragments arranged by size:
3322 1367 500
SspI AAT'ATT
Cuts at: 748 898 1863 2113 748
Size: 150 965 250 3824
Fragments arranged by size:
3824 965 250 150
StyI C'CwwG_G
Cuts at: 884 1823 2323 2677 884
Size: 939 500 354 3396
Fragments arranged by size:
3396 939 500 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2772 2905 3364 3573 2772
Size: 133 459 209 4388
Fragments arranged by size:
4388 459 209 133
TatI w'GTAC_w
Cuts at: 503 1533 1957 4704 503
Size: 1030 424 2747 988
Fragments arranged by size:
2747 1030 988 424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1690 1722 4706 4738 1690
Size: 32 2984 32 2141
Fragments arranged by size:
2984 2141 32 32
Tth111I GACn'n_nGTC
Cuts at: 2986 2986
Size: 5189
XbaI T'CTAG_A
Cuts at: 2220 2220
Size: 5189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 980 2684 980
Size: 1704 3485
XmaI C'CCGG_G
Cuts at: 2209 2209
Size: 5189
XmnI GAAnn'nnTTC
Cuts at: 899 930 1337 899
Size: 31 407 4751
Fragments arranged by size:
4751 407 31
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI FseI
FspAI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI