Restriction map for
pF1KB9960
(Circular) (Six-base) MAPSORT of: pF1KB9960.seq Check: 6672 from: 1 to: 5180
pF1KB9960 5180 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 980 2218 980
Size: 1238 3942
Acc65I G'GTAC_C
Cuts at: 2196 2196
Size: 5180
AclI AA'CG_TT
Cuts at: 5020 5020
Size: 5180
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 598 1087 1541 1723 2001 3005 3437 4184
Size: 294 489 454 182 278 1004 432 747
Cuts at: 4184 4452 304
Size: 268 1032
Fragments arranged by size:
1032 1004 747 489 454 432 294 278
268 182
AflIII A'CryG_T
Cuts at: 620 2532 3636 4613 620
Size: 1912 1104 977 1187
Fragments arranged by size:
1912 1187 1104 977
AgeI A'CCGG_T
Cuts at: 3568 3568
Size: 5180
AleI CACnn'nnGTG
Cuts at: 358 358
Size: 5180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 222 222
Size: 5180
AlwNI CAG_nnn'CTG
Cuts at: 165 2485 4052 165
Size: 2320 1567 1293
Fragments arranged by size:
2320 1567 1293
ApaLI G'TGCA_C
Cuts at: 3950 3950
Size: 5180
ApoI r'AATT_y
Cuts at: 1030 1444 1640 2184 4533 4789 1030
Size: 414 196 544 2349 256 1421
Fragments arranged by size:
2349 1421 544 414 256 196
AseI AT'TA_AT
Cuts at: 20 470 1306 20
Size: 450 836 3894
Fragments arranged by size:
3894 836 450
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
AvaI C'yCGr_G
Cuts at: 2200 2200
Size: 5180
BamHI G'GATC_C
Cuts at: 2205 2205
Size: 5180
BanI G'GyrC_C
Cuts at: 2196 2610 2858 2893 2196
Size: 414 248 35 4483
Fragments arranged by size:
4483 414 248 35
BanII G_rGCy'C
Cuts at: 596 716 2194 3224 596
Size: 120 1478 1030 2552
Fragments arranged by size:
2552 1478 1030 120
BbeI G_GCGC'C
Cuts at: 2614 2862 2614
Size: 248 4932
BciVI GTATCCnnnnn_n'
Cuts at: 342 3078 3839 5180 342
Size: 2736 761 1341 342
Fragments arranged by size:
2736 1341 761 342
BclI T'GATC_A
Cuts at: 627 1245 1458 2700 627
Size: 618 213 1242 3107
Fragments arranged by size:
3107 1242 618 213
BfrBI ATG'CAT
Cuts at: 994 994
Size: 5180
BglII A'GATC_T
Cuts at: 1389 2695 4732 1389
Size: 1306 2037 1837
Fragments arranged by size:
2037 1837 1306
BlpI GC'TnA_GC
Cuts at: 2292 2292
Size: 5180
Bme1580I G_kGCm'C
Cuts at: 2805 2898 3954 2805
Size: 93 1056 4031
Fragments arranged by size:
4031 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2392 2473 2808 4952 2392
Size: 81 335 2144 2620
Fragments arranged by size:
2620 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1340 3547 1340
Size: 2207 2973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1274 1078
Size: 196 4984
Bpu10I CC'TnA_GC
Cuts at: 1811 1811
Size: 5180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2356 3235 3727 4025 2356
Size: 879 492 298 3511
Fragments arranged by size:
3511 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1250 4884 1250
Size: 3634 1546
BsaAI yAC'GTr
Cuts at: 1180 2533 1180
Size: 1353 3827
BsaBI GATnn'nnATC
Cuts at: 6 1463 2694 4731 6
Size: 1457 1231 2037 455
Fragments arranged by size:
2037 1457 1231 455
BsaHI Gr'CG_yC
Cuts at: 2611 2859 3561 5057 2611
Size: 248 702 1496 2734
Fragments arranged by size:
2734 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1429 2369 2890 3568 3842 3989 4400 1429
Size: 940 521 678 274 147 411 2209
Fragments arranged by size:
2209 940 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1580 1610 1580
Size: 30 5150
BseYI C'CCAG_C
Cuts at: 135 1864 2605 3363 3940 4436 135
Size: 1729 741 758 577 496 879
Fragments arranged by size:
1729 879 758 741 577 496
BsiEI CG_ry'CG
Cuts at: 104 2722 2768 3976 104
Size: 2618 46 1208 1308
Fragments arranged by size:
2618 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 596 2194 2972 3162 3954 596
Size: 1598 778 190 792 1822
Fragments arranged by size:
1822 1598 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 596 716 2194 2805 2898 2972 3162 3224 3954
Size: 120 1478 611 93 74 190 62 730
Cuts at: 3954 596
Size: 1822
Fragments arranged by size:
1822 1478 730 611 190 120 93 74
62
BspEI T'CCGG_A
Cuts at: 4400 4400
Size: 5180
BspHI T'CATG_A
Cuts at: 4356 4356
Size: 5180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2230 2444 2746 3127 2230
Size: 214 302 381 4283
Fragments arranged by size:
4283 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3472 3526 5010 5175 50
Size: 3422 54 1484 165 55
Fragments arranged by size:
3422 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3092 3092
Size: 5180
BsrFI r'CCGG_y
Cuts at: 3178 3359 3568 3178
Size: 181 209 4790
Fragments arranged by size:
4790 209 181
BsrGI T'GTAC_A
Cuts at: 941 941
Size: 5180
BssSI C'ACGA_G
Cuts at: 3451 3809 3451
Size: 358 4822
BstAPI GCAn_nnn'nTGC
Cuts at: 2557 2557
Size: 5180
BstBI TT'CG_AA
Cuts at: 3541 3541
Size: 5180
BstEII G'GTnAC_C
Cuts at: 703 703
Size: 5180
BstXI CCAn_nnnn'nTGG
Cuts at: 1414 1414
Size: 5180
BstYI r'GATC_y
Cuts at: 1 1389 1697 2205 2672 2695 3030 3276 4288
Size: 1388 308 508 467 23 335 246 1012
Cuts at: 4288 4374 4386 4732 1
Size: 86 12 346 449
Fragments arranged by size:
1388 1012 508 467 449 346 335 308
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 981 981
Size: 5180
BtgI C'CryG_G
Cuts at: 107 107
Size: 5180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3130 3311 3474 3130
Size: 181 163 4836
Fragments arranged by size:
4836 181 163
BtsI GCAGTG_nn'
Cuts at: 1686 2148 2423 1686
Size: 462 275 4443
Fragments arranged by size:
4443 462 275
DraI TTT'AAA
Cuts at: 1265 1424 2179 1265
Size: 159 755 4266
Fragments arranged by size:
4266 755 159
DrdI GACnn_nn'nnGTC
Cuts at: 2886 3744 2886
Size: 858 4322
EaeI y'GGCC_r
Cuts at: 156 455 1613 1819 2765 2939 3330 3357 4702
Size: 299 1158 206 946 174 391 27 1345
Cuts at: 4702 156
Size: 634
Fragments arranged by size:
1345 1158 946 634 391 299 206 174
27
EagI C'GGCC_G
Cuts at: 2765 2765
Size: 5180
EarI CTCTTCn'nnn_
Cuts at: 758 1688 3203 3413 758
Size: 930 1515 210 2525
Fragments arranged by size:
2525 1515 930 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3388 3698 3844 4990 3388
Size: 310 146 1146 3578
Fragments arranged by size:
3578 1146 310 146
EcoICRI GAG'CTC
Cuts at: 594 2192 594
Size: 1598 3582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 598 1078 1087 1274 1541 1723 2001 3005
Size: 294 480 9 187 267 182 278 1004
Cuts at: 3005 3437 4184 4452 304
Size: 432 747 268 1032
Fragments arranged by size:
1032 1004 747 480 432 294 278 268
267 187 182 9
EcoNI CCTnn'n_nnAGG
Cuts at: 238 238
Size: 5180
EcoO109I rG'GnC_Cy
Cuts at: 1332 1545 2037 2319 1332
Size: 213 492 282 4193
Fragments arranged by size:
4193 492 282 213
EcoRI G'AATT_C
Cuts at: 2184 2184
Size: 5180
EcoRV GAT'ATC
Cuts at: 1770 1770
Size: 5180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3199 3199
Size: 5180
FspI TGC'GCA
Cuts at: 2961 2961
Size: 5180
HaeII r_GCGC'y
Cuts at: 2614 2862 3884 4863 2614
Size: 248 1022 979 2931
Fragments arranged by size:
2931 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 1682 1714 1759 1791 3022 3052 3054
Size: 32 884 32 45 32 1231 30 2
Cuts at: 3054 3084 766
Size: 30 2862
Fragments arranged by size:
2862 1231 884 45 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2219 2219
Size: 5180
KasI G'GCGC_C
Cuts at: 2610 2858 2610
Size: 248 4932
KpnI G_GTAC'C
Cuts at: 2200 2200
Size: 5180
MluI A'CGCG_T
Cuts at: 4613 4613
Size: 5180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3851 4035 3851
Size: 184 4996
MscI TGG'CCA
Cuts at: 158 457 1615 1821 2941 158
Size: 299 1158 206 1120 2397
Fragments arranged by size:
2397 1158 1120 299 206
MslI CAynn'nnrTG
Cuts at: 358 974 3169 3296 358
Size: 616 2195 127 2242
Fragments arranged by size:
2242 2195 616 127
MspA1I CmG'CkG
Cuts at: 165 206 358 2291 2605 2965 3978 4223 165
Size: 41 152 1933 314 360 1013 245 1122
Fragments arranged by size:
1933 1122 1013 360 314 245 152 41
NaeI GCC'GGC
Cuts at: 3361 3361
Size: 5180
NarI GG'CG_CC
Cuts at: 2611 2859 2611
Size: 248 4932
NcoI C'CATG_G
Cuts at: 107 107
Size: 5180
NdeI CA'TA_TG
Cuts at: 1372 1372
Size: 5180
NgoMIV G'CCGG_C
Cuts at: 3359 3359
Size: 5180
NsiI A_TGCA'T
Cuts at: 996 996
Size: 5180
NspI r_CATG'y
Cuts at: 624 2233 2733 3640 624
Size: 1609 500 907 2164
Fragments arranged by size:
2164 1609 907 500
PciI A'CATG_T
Cuts at: 620 3636 620
Size: 3016 2164
PflMI CCAn_nnn'nTGG
Cuts at: 1605 1605
Size: 5180
PmeI GTTT'AAAC
Cuts at: 2179 2179
Size: 5180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4319 4351 4319
Size: 32 5148
PpuMI rG'GwC_Cy
Cuts at: 1332 1545 1332
Size: 213 4967
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 110 142 500 532 110
Size: 32 358 32 4758
Fragments arranged by size:
4758 358 32 32
PstI C_TGCA'G
Cuts at: 211 2227 2912 211
Size: 2016 685 2479
Fragments arranged by size:
2479 2016 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5180
PvuII CAG'CTG
Cuts at: 206 358 2605 2965 206
Size: 152 2247 360 2421
Fragments arranged by size:
2421 2247 360 152
RsrII CG'GwC_CG
Cuts at: 3375 3375
Size: 5180
SacI G_AGCT'C
Cuts at: 596 2194 596
Size: 1598 3582
SalI G'TCGA_C
Cuts at: 2217 2217
Size: 5180
SapI GCTCTTCn'nnn_
Cuts at: 3203 3413 3203
Size: 210 4970
SbfI CC_TGCA'GG
Cuts at: 2227 2227
Size: 5180
ScaI AGT'ACT
Cuts at: 538 4697 538
Size: 4159 1021
SfcI C'TryA_G
Cuts at: 33 207 2223 2908 3901 4092 33
Size: 174 2016 685 993 191 1121
Fragments arranged by size:
2016 1121 993 685 191 174
SfoI GGC'GCC
Cuts at: 2612 2860 2612
Size: 248 4932
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5180
SmaI CCC'GGG
Cuts at: 2202 2202
Size: 5180
SmlI C'TyrA_G
Cuts at: 2335 3250 3742 4004 2335
Size: 915 492 262 3511
Fragments arranged by size:
3511 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5180
SphI G_CATG'C
Cuts at: 2233 2733 2233
Size: 500 4680
SspI AAT'ATT
Cuts at: 487 487
Size: 5180
StyI C'CwwG_G
Cuts at: 107 2314 2668 107
Size: 2207 354 2619
Fragments arranged by size:
2619 2207 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1742 2059 2763 2896 3355 3564 1742
Size: 317 704 133 459 209 3358
Fragments arranged by size:
3358 704 459 317 209 133
TatI w'GTAC_w
Cuts at: 536 941 1410 4695 536
Size: 405 469 3285 1021
Fragments arranged by size:
3285 1021 469 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 638 670 676 708 4697 4729 638
Size: 32 6 32 3989 32 1089
Fragments arranged by size:
3989 1089 32 32 32 6
Tth111I GACn'n_nGTC
Cuts at: 2977 2977
Size: 5180
XbaI T'CTAG_A
Cuts at: 1392 2211 1392
Size: 819 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2675 2675
Size: 5180
XmaI C'CCGG_G
Cuts at: 2200 2200
Size: 5180
XmnI GAAnn'nnTTC
Cuts at: 849 1631 849
Size: 782 4398
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmI BsmBI BssHII Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI