Restriction map for
pF1KB9949
(Circular) (Six-base) MAPSORT of: pF1KB9949.seq Check: 435 from: 1 to: 4709
pF1KB9949 4709 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1148 1148
Size: 4709
AatII G_ACGT'C
Cuts at: 669 669
Size: 4709
AccI GT'mk_AC
Cuts at: 1584 1747 1584
Size: 163 4546
Acc65I G'GTAC_C
Cuts at: 1488 1725 1488
Size: 237 4472
AclI AA'CG_TT
Cuts at: 1539 4549 1539
Size: 3010 1699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 926 2534 2966 3713 3981 383
Size: 543 1608 432 747 268 1111
Fragments arranged by size:
1608 1111 747 543 432 268
AflIII A'CryG_T
Cuts at: 2061 3165 4142 2061
Size: 1104 977 2628
Fragments arranged by size:
2628 1104 977
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AlwNI CAG_nnn'CTG
Cuts at: 183 2014 3581 183
Size: 1831 1567 1311
Fragments arranged by size:
1831 1567 1311
ApaI G_GGCC'C
Cuts at: 1623 1623
Size: 4709
ApaLI G'TGCA_C
Cuts at: 3479 3479
Size: 4709
ApoI r'AATT_y
Cuts at: 1151 1264 1713 4062 4318 1151
Size: 113 449 2349 256 1542
Fragments arranged by size:
2349 1542 449 256 113
AseI AT'TA_AT
Cuts at: 20 323 20
Size: 303 4406
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 1729 1729
Size: 4709
BamHI G'GATC_C
Cuts at: 1734 1734
Size: 4709
BanI G'GyrC_C
Cuts at: 1159 1315 1488 1725 2139 2387 2422 1159
Size: 156 173 237 414 248 35 3446
Fragments arranged by size:
3446 414 248 237 173 156 35
BanII G_rGCy'C
Cuts at: 1098 1623 1723 2753 1098
Size: 525 100 1030 3054
Fragments arranged by size:
3054 1030 525 100
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 1669 1669
Size: 4709
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4675
BciVI GTATCCnnnnn_n'
Cuts at: 261 2607 3368 4709 261
Size: 2346 761 1341 261
Fragments arranged by size:
2346 1341 761 261
BclI T'GATC_A
Cuts at: 608 2229 608
Size: 1621 3088
BfrBI ATG'CAT
Cuts at: 335 335
Size: 4709
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 1821 1821
Size: 4709
Bme1580I G_kGCm'C
Cuts at: 305 1298 1318 1623 2334 2427 3483 305
Size: 993 20 305 711 93 1056 1531
Fragments arranged by size:
1531 1056 993 711 305 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 215 1921 2002 2337 4481 215
Size: 1706 81 335 2144 443
Fragments arranged by size:
2144 1706 443 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 1620 603
Size: 1017 3692
Bpu10I CC'TnA_GC
Cuts at: 113 255 297 113
Size: 142 42 4525
Fragments arranged by size:
4525 142 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 1885 2764 3256 3554 642
Size: 1243 879 492 298 1797
Fragments arranged by size:
1797 1243 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1184 4413 1184
Size: 3229 1480
BsaAI yAC'GTr
Cuts at: 2062 2062
Size: 4709
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 666 2140 2388 3090 4586 666
Size: 1474 248 702 1496 789
Fragments arranged by size:
1496 1474 789 702 248
BsaWI w'CCGG_w
Cuts at: 1898 2419 3097 3371 3518 3929 1898
Size: 521 678 274 147 411 2678
Fragments arranged by size:
2678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1439 1469 1439
Size: 30 4679
BseYI C'CCAG_C
Cuts at: 455 1616 2134 2892 3469 3965 455
Size: 1161 518 758 577 496 1199
Fragments arranged by size:
1199 1161 758 577 518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1126
Size: 4709
BsiEI CG_ry'CG
Cuts at: 104 2251 2297 3505 104
Size: 2147 46 1208 1308
Fragments arranged by size:
2147 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1723 2501 2691 3483 1723
Size: 778 190 792 2949
Fragments arranged by size:
2949 792 778 190
BsmI GAATG_Cn'
Cuts at: 557 1618 557
Size: 1061 3648
Bsp1286I G_dGCh'C
Cuts at: 305 1098 1298 1318 1623 1723 2334 2427 2501
Size: 793 200 20 305 100 611 93 74
Cuts at: 2501 2691 2753 3483 305
Size: 190 62 730 1531
Fragments arranged by size:
1531 793 730 611 305 200 190 100
93 74 62 20
BspEI T'CCGG_A
Cuts at: 3929 3929
Size: 4709
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1148 1759 1973 2275 2656 1148
Size: 611 214 302 381 3201
Fragments arranged by size:
3201 611 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 348 1238 2621 348
Size: 890 1383 2436
Fragments arranged by size:
2436 1383 890
BsrFI r'CCGG_y
Cuts at: 2707 2888 3097 2707
Size: 181 209 4319
Fragments arranged by size:
4319 209 181
BssSI C'ACGA_G
Cuts at: 164 2980 3338 164
Size: 2816 358 1535
Fragments arranged by size:
2816 1535 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2086 2086
Size: 4709
BstBI TT'CG_AA
Cuts at: 3070 3070
Size: 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 1048 1048
Size: 4709
BstYI r'GATC_y
Cuts at: 1 421 1051 1734 2201 2224 2559 2805 3817
Size: 420 630 683 467 23 335 246 1012
Cuts at: 3817 3903 3915 4261 1
Size: 86 12 346 449
Fragments arranged by size:
1012 683 630 467 449 420 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2659 2840 3003 2659
Size: 181 163 4365
Fragments arranged by size:
4365 181 163
BtsI GCAGTG_nn'
Cuts at: 517 1952 517
Size: 1435 3274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 849 884 849
Size: 35 4674
DraI TTT'AAA
Cuts at: 284 1708 284
Size: 1424 3285
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3273 2415
Size: 858 3851
EaeI y'GGCC_r
Cuts at: 1546 2294 2468 2859 2886 4231 1546
Size: 748 174 391 27 1345 2024
Fragments arranged by size:
2024 1345 748 391 174 27
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2917 3227 3373 4519 2917
Size: 310 146 1146 3107
Fragments arranged by size:
3107 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1721 1721
Size: 4709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 603 926 1620 2534 2966 3713 3981 383
Size: 220 323 694 914 432 747 268 1111
Fragments arranged by size:
1111 914 747 694 432 323 268 220
EcoO109I rG'GnC_Cy
Cuts at: 1848 1848
Size: 4709
EcoRI G'AATT_C
Cuts at: 1713 1713
Size: 4709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4709
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 2143 2391 3413 4392 2143
Size: 248 1022 979 2460
Fragments arranged by size:
2460 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1416 1448 2551 2581 2583 2613 1416
Size: 32 1103 30 2 30 3512
Fragments arranged by size:
3512 1103 32 30 30 2
HincII GTy'rAC
Cuts at: 1748 1748
Size: 4709
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1492 1729 1492
Size: 237 4472
MfeI C'AATT_G
Cuts at: 1178 1468 1550 1178
Size: 290 82 4337
Fragments arranged by size:
4337 290 82
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 463 693 3380 3564 248
Size: 215 230 2687 184 1393
Fragments arranged by size:
2687 1393 230 215 184
MscI TGG'CCA
Cuts at: 2470 2470
Size: 4709
MslI CAynn'nnrTG
Cuts at: 208 583 1046 2698 2825 208
Size: 375 463 1652 127 2092
Fragments arranged by size:
2092 1652 463 375 127
MspA1I CmG'CkG
Cuts at: 623 917 1120 1820 2134 2494 3507 3752 623
Size: 294 203 700 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 700 360 314 294 245 203
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NsiI A_TGCA'T
Cuts at: 337 337
Size: 4709
NspI r_CATG'y
Cuts at: 335 1762 2262 3169 335
Size: 1427 500 907 1875
Fragments arranged by size:
1875 1427 907 500
PciI A'CATG_T
Cuts at: 3165 3165
Size: 4709
PfoI T'CCnGG_A
Cuts at: 1009 1009
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4677
PspOMI G'GGCC_C
Cuts at: 1619 1619
Size: 4709
PstI C_TGCA'G
Cuts at: 1756 2441 1756
Size: 685 4024
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 623 917 1120 2134 2494 623
Size: 294 203 1014 360 2838
Fragments arranged by size:
2838 1014 360 294 203
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 1723 1723
Size: 4709
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SfcI C'TryA_G
Cuts at: 33 1398 1752 2437 3430 3621 33
Size: 1365 354 685 993 191 1121
Fragments arranged by size:
1365 1121 993 685 354 191
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1731 1731
Size: 4709
SmlI C'TyrA_G
Cuts at: 657 1864 2779 3271 3533 657
Size: 1207 915 492 262 1833
Fragments arranged by size:
1833 1207 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1762 2262 1762
Size: 500 4209
StyI C'CwwG_G
Cuts at: 1164 1843 2197 1164
Size: 679 354 3676
Fragments arranged by size:
3676 679 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1177 2292 2425 2884 3093 1177
Size: 1115 133 459 209 2793
Fragments arranged by size:
2793 1115 459 209 133
TatI w'GTAC_w
Cuts at: 4224 4224
Size: 4709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1439 1471 4226 4258 1439
Size: 32 2755 32 1890
Fragments arranged by size:
2755 1890 32 32
Tth111I GACn'n_nGTC
Cuts at: 2506 2506
Size: 4709
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1326 2204 1326
Size: 878 3831
XmaI C'CCGG_G
Cuts at: 1729 1729
Size: 4709
XmnI GAAnn'nnTTC
Cuts at: 892 892
Size: 4709
ZraI GAC'GTC
Cuts at: 667 667
Size: 4709
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BglI BmgBI BmtI BseRI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI