Restriction map for
pF1KB9940
(Circular) (Six-base) MAPSORT of: pF1KB9940.seq Check: 184 from: 1 to: 5084
pF1KB9940 5084 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2122 2122
Size: 5084
Acc65I G'GTAC_C
Cuts at: 1265 2100 1265
Size: 835 4249
AclI AA'CG_TT
Cuts at: 4924 4924
Size: 5084
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 974 2909 3341 4088 4356 928
Size: 46 1935 432 747 268 1656
Fragments arranged by size:
1935 1656 747 432 268 46
AflIII A'CryG_T
Cuts at: 1044 1256 2436 3540 4517 1044
Size: 212 1180 1104 977 1611
Fragments arranged by size:
1611 1180 1104 977 212
AgeI A'CCGG_T
Cuts at: 3472 3472
Size: 5084
AlwNI CAG_nnn'CTG
Cuts at: 1336 2389 3956 1336
Size: 1053 1567 2464
Fragments arranged by size:
2464 1567 1053
ApaI G_GGCC'C
Cuts at: 972 972
Size: 5084
ApaLI G'TGCA_C
Cuts at: 608 1529 3854 608
Size: 921 2325 1838
Fragments arranged by size:
2325 1838 921
ApoI r'AATT_y
Cuts at: 1235 1297 1371 1469 1951 2088 4437 4693 1235
Size: 62 74 98 482 137 2349 256 1626
Fragments arranged by size:
2349 1626 482 256 137 98 74 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 5084
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
AvaI C'yCGr_G
Cuts at: 2104 2104
Size: 5084
AvrII C'CTAG_G
Cuts at: 1425 1425
Size: 5084
BamHI G'GATC_C
Cuts at: 393 2109 393
Size: 1716 3368
BanI G'GyrC_C
Cuts at: 1265 2100 2514 2762 2797 1265
Size: 835 414 248 35 3552
Fragments arranged by size:
3552 835 414 248 35
BanII G_rGCy'C
Cuts at: 148 972 1942 2098 3128 148
Size: 824 970 156 1030 2104
Fragments arranged by size:
2104 1030 970 824 156
BbeI G_GCGC'C
Cuts at: 2518 2766 2518
Size: 248 4836
BbsI GAAGACnn'nnnn_
Cuts at: 962 1080 1104 962
Size: 118 24 4942
Fragments arranged by size:
4942 118 24
BbvCI CC'TCA_GC
Cuts at: 1647 1647
Size: 5084
BciVI GTATCCnnnnn_n'
Cuts at: 283 2982 3743 5084 283
Size: 2699 761 1341 283
Fragments arranged by size:
2699 1341 761 283
BclI T'GATC_A
Cuts at: 680 1823 2604 680
Size: 1143 781 3160
Fragments arranged by size:
3160 1143 781
BfrBI ATG'CAT
Cuts at: 1606 1606
Size: 5084
BglII A'GATC_T
Cuts at: 2599 4636 2599
Size: 2037 3047
BlpI GC'TnA_GC
Cuts at: 2196 2196
Size: 5084
Bme1580I G_kGCm'C
Cuts at: 612 972 1533 2709 2802 3858 612
Size: 360 561 1176 93 1056 1838
Fragments arranged by size:
1838 1176 1056 561 360 93
BmrI ACTGGGnnnn_n'
Cuts at: 2296 2377 2712 4856 2296
Size: 81 335 2144 2524
Fragments arranged by size:
2524 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 5084
Bpu10I CC'TnA_GC
Cuts at: 935 1647 935
Size: 712 4372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2260 3139 3631 3929 2260
Size: 879 492 298 3415
Fragments arranged by size:
3415 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 917 4788 917
Size: 3871 1213
BsaAI yAC'GTr
Cuts at: 1074 2437 1074
Size: 1363 3721
BsaBI GATnn'nnATC
Cuts at: 6 1288 2598 4635 6
Size: 1282 1310 2037 455
Fragments arranged by size:
2037 1310 1282 455
BsaHI Gr'CG_yC
Cuts at: 2027 2515 2763 3465 4961 2027
Size: 488 248 702 1496 2150
Fragments arranged by size:
2150 1496 702 488 248
BsaWI w'CCGG_w
Cuts at: 419 2273 2794 3472 3746 3893 4304 419
Size: 1854 521 678 274 147 411 1199
Fragments arranged by size:
1854 1199 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 546 576 915 945 1325 1355 546
Size: 30 339 30 380 30 4275
Fragments arranged by size:
4275 380 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 964 120
Size: 844 4240
BseYI C'CCAG_C
Cuts at: 368 1768 2509 3267 3844 4340 368
Size: 1400 741 758 577 496 1112
Fragments arranged by size:
1400 1112 758 741 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 287 1626 168
Size: 119 1339 3626
Fragments arranged by size:
3626 1339 119
BsiEI CG_ry'CG
Cuts at: 104 2626 2672 3880 104
Size: 2522 46 1208 1308
Fragments arranged by size:
2522 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 148 612 639 1533 2098 2876 3066 3858 148
Size: 464 27 894 565 778 190 792 1374
Fragments arranged by size:
1374 894 792 778 565 464 190 27
BsmI GAATG_Cn'
Cuts at: 1608 1686 1608
Size: 78 5006
Bsp1286I G_dGCh'C
Cuts at: 148 612 639 972 1533 1942 2098 2709 2802
Size: 464 27 333 561 409 156 611 93
Cuts at: 2802 2876 3066 3128 3858 148
Size: 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 561 464 409 333 190
156 93 74 62 27
BspEI T'CCGG_A
Cuts at: 4304 4304
Size: 5084
BspHI T'CATG_A
Cuts at: 1166 4260 1166
Size: 3094 1990
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2134 2348 2650 3031 2134
Size: 214 302 381 4187
Fragments arranged by size:
4187 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3376 3430 4914 5079 50
Size: 3326 54 1484 165 55
Fragments arranged by size:
3326 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1857 2009 2996 1857
Size: 152 987 3945
Fragments arranged by size:
3945 987 152
BsrFI r'CCGG_y
Cuts at: 1192 3082 3263 3472 1192
Size: 1890 181 209 2804
Fragments arranged by size:
2804 1890 209 181
BssSI C'ACGA_G
Cuts at: 3355 3713 3355
Size: 358 4726
BstAPI GCAn_nnn'nTGC
Cuts at: 265 1559 1657 2074 2461 265
Size: 1294 98 417 387 2888
Fragments arranged by size:
2888 1294 417 387 98
BstBI TT'CG_AA
Cuts at: 3445 3445
Size: 5084
BstXI CCAn_nnnn'nTGG
Cuts at: 1078 1078
Size: 5084
BstYI r'GATC_y
Cuts at: 1 393 833 1444 2109 2576 2599 2934 3180
Size: 392 440 611 665 467 23 335 246
Cuts at: 3180 4192 4278 4290 4636 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 665 611 467 449 440 392 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 196 107
Size: 89 4995
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 613 1044 3034 3215 3378 613
Size: 431 1990 181 163 2319
Fragments arranged by size:
2319 1990 431 181 163
BtsI GCAGTG_nn'
Cuts at: 1102 2327 1102
Size: 1225 3859
DraI TTT'AAA
Cuts at: 1595 2083 1595
Size: 488 4596
DraIII CAC_nnn'GTG
Cuts at: 445 1537 445
Size: 1092 3992
DrdI GACnn_nn'nnGTC
Cuts at: 703 2790 3648 703
Size: 2087 858 2139
Fragments arranged by size:
2139 2087 858
EaeI y'GGCC_r
Cuts at: 2669 2843 3234 3261 4606 2669
Size: 174 391 27 1345 3147
Fragments arranged by size:
3147 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2669 2669
Size: 5084
EarI CTCTTCn'nnn_
Cuts at: 214 223 3107 3317 214
Size: 9 2884 210 1981
Fragments arranged by size:
2884 1981 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 114 123 3292 3602 3748 4894 114
Size: 9 3169 310 146 1146 304
Fragments arranged by size:
3169 1146 310 304 146 9
EcoICRI GAG'CTC
Cuts at: 146 2096 146
Size: 1950 3134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 928 974 2909 3341 4088 4356 190
Size: 738 46 1935 432 747 268 918
Fragments arranged by size:
1935 918 747 738 432 268 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1735 1735
Size: 5084
EcoO109I rG'GnC_Cy
Cuts at: 675 969 2223 675
Size: 294 1254 3536
Fragments arranged by size:
3536 1254 294
EcoRI G'AATT_C
Cuts at: 1951 2088 1951
Size: 137 4947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 979 1677 3103 979
Size: 698 1426 2960
Fragments arranged by size:
2960 1426 698
FspI TGC'GCA
Cuts at: 1039 2865 1039
Size: 1826 3258
HaeII r_GCGC'y
Cuts at: 2518 2766 3788 4767 2518
Size: 248 1022 979 2835
Fragments arranged by size:
2835 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2926 2956 2958 2988 2926
Size: 30 2 30 5022
Fragments arranged by size:
5022 30 30 2
HincII GTy'rAC
Cuts at: 2123 2123
Size: 5084
KasI G'GCGC_C
Cuts at: 2514 2762 2514
Size: 248 4836
KpnI G_GTAC'C
Cuts at: 1269 2104 1269
Size: 835 4249
MluI A'CGCG_T
Cuts at: 4517 4517
Size: 5084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 314 1140 3755 3939 314
Size: 826 2615 184 1459
Fragments arranged by size:
2615 1459 826 184
MscI TGG'CCA
Cuts at: 2845 2845
Size: 5084
MslI CAynn'nnrTG
Cuts at: 229 632 1672 3073 3200 229
Size: 403 1040 1401 127 2113
Fragments arranged by size:
2113 1401 1040 403 127
MspA1I CmG'CkG
Cuts at: 168 372 2195 2509 2869 3882 4127 168
Size: 204 1823 314 360 1013 245 1125
Fragments arranged by size:
1823 1125 1013 360 314 245 204
NaeI GCC'GGC
Cuts at: 3265 3265
Size: 5084
NarI GG'CG_CC
Cuts at: 2515 2763 2515
Size: 248 4836
NcoI C'CATG_G
Cuts at: 107 196 107
Size: 89 4995
NgoMIV G'CCGG_C
Cuts at: 3263 3263
Size: 5084
NsiI A_TGCA'T
Cuts at: 1608 1608
Size: 5084
NspI r_CATG'y
Cuts at: 1260 1313 2137 2637 3544 1260
Size: 53 824 500 907 2800
Fragments arranged by size:
2800 907 824 500 53
PciI A'CATG_T
Cuts at: 1256 3540 1256
Size: 2284 2800
PflMI CCAn_nnn'nTGG
Cuts at: 352 1275 352
Size: 923 4161
PmeI GTTT'AAAC
Cuts at: 2083 2083
Size: 5084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 4223
Size: 32 5052
PshAI GACnn'nnGTC
Cuts at: 282 282
Size: 5084
PspOMI G'GGCC_C
Cuts at: 968 968
Size: 5084
PstI C_TGCA'G
Cuts at: 121 1253 1756 2131 2816 121
Size: 1132 503 375 685 2389
Fragments arranged by size:
2389 1132 685 503 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5084
PvuII CAG'CTG
Cuts at: 2509 2869 2509
Size: 360 4724
RsrII CG'GwC_CG
Cuts at: 3279 3279
Size: 5084
SacI G_AGCT'C
Cuts at: 148 2098 148
Size: 1950 3134
SalI G'TCGA_C
Cuts at: 2121 2121
Size: 5084
SapI GCTCTTCn'nnn_
Cuts at: 214 3107 3317 214
Size: 2893 210 1981
Fragments arranged by size:
2893 1981 210
SbfI CC_TGCA'GG
Cuts at: 2131 2131
Size: 5084
ScaI AGT'ACT
Cuts at: 824 4601 824
Size: 3777 1307
SfcI C'TryA_G
Cuts at: 33 117 434 995 1249 1752 2127 2812 3805
Size: 84 317 561 254 503 375 685 993
Cuts at: 3805 3996 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 561 503 375 317 254
191 84
SfoI GGC'GCC
Cuts at: 2516 2764 2516
Size: 248 4836
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5084
SmaI CCC'GGG
Cuts at: 2106 2106
Size: 5084
SmlI C'TyrA_G
Cuts at: 2239 3154 3646 3908 2239
Size: 915 492 262 3415
Fragments arranged by size:
3415 915 492 262
SpeI A'CTAG_T
Cuts at: 67 279 774 67
Size: 212 495 4377
Fragments arranged by size:
4377 495 212
SphI G_CATG'C
Cuts at: 2137 2637 2137
Size: 500 4584
StyI C'CwwG_G
Cuts at: 107 196 460 1425 1731 2218 2572 107
Size: 89 264 965 306 487 354 2619
Fragments arranged by size:
2619 965 487 354 306 264 89
SwaI ATTT'AAAT
Cuts at: 1595 1595
Size: 5084
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 382 1143 1800 2667 2800 3259 3468 382
Size: 761 657 867 133 459 209 1998
Fragments arranged by size:
1998 867 761 657 459 209 133
TatI w'GTAC_w
Cuts at: 822 879 1157 1253 4599 822
Size: 57 278 96 3346 1307
Fragments arranged by size:
3346 1307 278 96 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 4601 4633 546
Size: 32 4023 32 997
Fragments arranged by size:
4023 997 32 32
Tth111I GACn'n_nGTC
Cuts at: 2881 2881
Size: 5084
XbaI T'CTAG_A
Cuts at: 2115 2115
Size: 5084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 792 2579 792
Size: 1787 3297
XmaI C'CCGG_G
Cuts at: 2104 2104
Size: 5084
XmnI GAAnn'nnTTC
Cuts at: 1301 1301
Size: 5084
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI XhoI ZraI