Restriction map for
pF1KB9937
(Circular) (Six-base) MAPSORT of: pF1KB9937.seq Check: 9132 from: 1 to: 4679
pF1KB9937 4679 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 890 890
Size: 4679
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 839 931 1695 839
Size: 92 764 3823
Fragments arranged by size:
3823 764 92
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2504 2936 3683 3951 829
Size: 1675 432 747 268 1557
Fragments arranged by size:
1675 1557 747 432 268
AfeI AGC'GCT
Cuts at: 135 1072 1130 1363 135
Size: 937 58 233 3451
Fragments arranged by size:
3451 937 233 58
AflIII A'CryG_T
Cuts at: 2031 3135 4112 2031
Size: 1104 977 2598
Fragments arranged by size:
2598 1104 977
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 1626 1984 3551 1626
Size: 358 1567 2754
Fragments arranged by size:
2754 1567 358
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 474 805 1683 4032 4288 474
Size: 331 878 2349 256 865
Fragments arranged by size:
2349 878 865 331 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 208 1468 1481 1699 208
Size: 1260 13 218 3188
Fragments arranged by size:
3188 1260 218 13
BamHI G'GATC_C
Cuts at: 335 1704 335
Size: 1369 3310
BanI G'GyrC_C
Cuts at: 253 839 931 1695 2109 2357 2392 253
Size: 586 92 764 414 248 35 2540
Fragments arranged by size:
2540 764 586 414 248 92 35
BanII G_rGCy'C
Cuts at: 1177 1292 1693 2723 1177
Size: 115 401 1030 3133
Fragments arranged by size:
3133 1030 401 115
BbeI G_GCGC'C
Cuts at: 257 2113 2361 257
Size: 1856 248 2575
Fragments arranged by size:
2575 1856 248
BbsI GAAGACnn'nnnn_
Cuts at: 1357 1357
Size: 4679
BbvCI CC'TCA_GC
Cuts at: 1220 1220
Size: 4679
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3338 4679 2577
Size: 761 1341 2577
Fragments arranged by size:
2577 1341 761
BclI T'GATC_A
Cuts at: 1586 2199 1586
Size: 613 4066
BfrBI ATG'CAT
Cuts at: 589 589
Size: 4679
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1286 1791 1286
Size: 505 4174
Bme1580I G_kGCm'C
Cuts at: 1206 2304 2397 3453 1206
Size: 1098 93 1056 2432
Fragments arranged by size:
2432 1098 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1137 1647 1891 1972 2307 4451 1137
Size: 510 244 81 335 2144 1365
Fragments arranged by size:
2144 1365 510 335 244 81
BmtI G_CTAG'C
Cuts at: 436 1129 436
Size: 693 3986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 167 726 3046 167
Size: 559 2320 1800
Fragments arranged by size:
2320 1800 559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1030 190
Size: 840 3839
Bpu10I CC'TnA_GC
Cuts at: 1220 1220
Size: 4679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1618 1855 2734 3226 3524 946
Size: 672 237 879 492 298 2101
Fragments arranged by size:
2101 879 672 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 961 4383 961
Size: 3422 1257
BsaAI yAC'GTr
Cuts at: 922 1584 2032 922
Size: 662 448 3569
Fragments arranged by size:
3569 662 448
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 254 887 2110 2358 3060 4556 112
Size: 142 633 1223 248 702 1496 235
Fragments arranged by size:
1496 1223 702 633 248 235 142
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 304 334 1042 1072 163
Size: 30 111 30 708 30 3770
Fragments arranged by size:
3770 708 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 517 949 1051 1078 449
Size: 68 432 102 27 4050
Fragments arranged by size:
4050 432 102 68 27
BseYI C'CCAG_C
Cuts at: 214 488 1211 2104 2862 3439 3935 214
Size: 274 723 893 758 577 496 958
Fragments arranged by size:
958 893 758 723 577 496 274
BsiEI CG_ry'CG
Cuts at: 104 194 2221 2267 3475 104
Size: 90 2027 46 1208 1308
Fragments arranged by size:
2027 1308 1208 90 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1292 1693 2471 2661 3453 1177
Size: 115 401 778 190 792 2403
Fragments arranged by size:
2403 792 778 401 190 115
BsmI GAATG_Cn'
Cuts at: 591 591
Size: 4679
Bsp1286I G_dGCh'C
Cuts at: 1177 1206 1292 1693 2304 2397 2471 2661 2723
Size: 29 86 401 611 93 74 190 62
Cuts at: 2723 3453 1177
Size: 730 2403
Fragments arranged by size:
2403 730 611 401 190 93 86 74
62 29
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 3855 3855
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1729 1943 2245 2626 1729
Size: 214 302 381 3782
Fragments arranged by size:
3782 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2591 2591
Size: 4679
BsrFI r'CCGG_y
Cuts at: 250 2677 2858 3067 250
Size: 2427 181 209 1862
Fragments arranged by size:
2427 1862 209 181
BssSI C'ACGA_G
Cuts at: 2950 3308 2950
Size: 358 4321
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2056
Size: 4679
BstBI TT'CG_AA
Cuts at: 481 3040 481
Size: 2559 2120
BstYI r'GATC_y
Cuts at: 1 335 366 1463 1704 2171 2194 2529 2775
Size: 334 31 1097 241 467 23 335 246
Cuts at: 2775 3787 3873 3885 4231 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1097 1012 467 449 346 335 334 246
241 86 31 23 12
Bsu36I CC'TnA_GG
Cuts at: 461 1655 461
Size: 1194 3485
BtgI C'CryG_G
Cuts at: 107 257 926 107
Size: 150 669 3860
Fragments arranged by size:
3860 669 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1384 1922 1384
Size: 538 4141
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DrdI GACnn_nn'nnGTC
Cuts at: 886 2385 3243 886
Size: 1499 858 2322
Fragments arranged by size:
2322 1499 858
EaeI y'GGCC_r
Cuts at: 191 1316 2264 2438 2829 2856 4201 191
Size: 1125 948 174 391 27 1345 669
Fragments arranged by size:
1345 1125 948 669 391 174 27
EagI C'GGCC_G
Cuts at: 191 2264 191
Size: 2073 2606
EarI CTCTTCn'nnn_
Cuts at: 458 742 905 2702 2912 458
Size: 284 163 1797 210 2225
Fragments arranged by size:
2225 1797 284 210 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 143 2887 3197 3343 4489 143
Size: 2744 310 146 1146 333
Fragments arranged by size:
2744 1146 333 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1290 1691 1175
Size: 115 401 4163
Fragments arranged by size:
4163 401 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 829 1030 2504 2936 3683 3951 190
Size: 639 201 1474 432 747 268 918
Fragments arranged by size:
1474 918 747 639 432 268 201
EcoNI CCTnn'n_nnAGG
Cuts at: 1654 1654
Size: 4679
EcoO109I rG'GnC_Cy
Cuts at: 879 1818 879
Size: 939 3740
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1666 2698 1666
Size: 1032 3647
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 137 257 1074 1132 1365 2113 2361 3383 4362
Size: 120 817 58 233 748 248 1022 979
Cuts at: 4362 137
Size: 454
Fragments arranged by size:
1022 979 817 748 454 248 233 120
58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 843 875 1462 1494 2521 2551 2553
Size: 32 648 32 587 32 1027 30 2
Cuts at: 2553 2583 163
Size: 30 2259
Fragments arranged by size:
2259 1027 648 587 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1110 1718 1110
Size: 608 4071
HindIII A'AGCT_T
Cuts at: 621 694 621
Size: 73 4606
KasI G'GCGC_C
Cuts at: 253 2109 2357 253
Size: 1856 248 2575
Fragments arranged by size:
2575 1856 248
KpnI G_GTAC'C
Cuts at: 843 935 1699 843
Size: 92 764 3823
Fragments arranged by size:
3823 764 92
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3350 3534 3350
Size: 184 4495
MscI TGG'CCA
Cuts at: 1318 2440 1318
Size: 1122 3557
MslI CAynn'nnrTG
Cuts at: 760 1009 2668 2795 760
Size: 249 1659 127 2644
Fragments arranged by size:
2644 1659 249 127
MspA1I CmG'CkG
Cuts at: 259 1215 1626 1790 2104 2464 3477 3722 259
Size: 956 411 164 314 360 1013 245 1216
Fragments arranged by size:
1216 1013 956 411 360 314 245 164
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 254 2110 2358 254
Size: 1856 248 2575
Fragments arranged by size:
2575 1856 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4679
NdeI CA'TA_TG
Cuts at: 591 591
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NheI G'CTAG_C
Cuts at: 432 1125 432
Size: 693 3986
NsiI A_TGCA'T
Cuts at: 591 591
Size: 4679
NspI r_CATG'y
Cuts at: 1090 1732 2232 3139 1090
Size: 642 500 907 2630
Fragments arranged by size:
2630 907 642 500
PciI A'CATG_T
Cuts at: 3135 3135
Size: 4679
PfoI T'CCnGG_A
Cuts at: 1046 1046
Size: 4679
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PmlI CAC'GTG
Cuts at: 922 1584 922
Size: 662 4017
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PpuMI rG'GwC_Cy
Cuts at: 879 879
Size: 4679
PspXI vC'TCGA_Gb
Cuts at: 1468 1468
Size: 4679
PstI C_TGCA'G
Cuts at: 804 1391 1448 1726 2411 804
Size: 587 57 278 685 3072
Fragments arranged by size:
3072 685 587 278 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 1215 1626 2104 2464 1215
Size: 411 478 360 3430
Fragments arranged by size:
3430 478 411 360
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1177 1292 1693 1177
Size: 115 401 4163
Fragments arranged by size:
4163 401 115
SacII CC_GC'GG
Cuts at: 260 260
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 742 905 2702 2912 742
Size: 163 1797 210 2509
Fragments arranged by size:
2509 1797 210 163
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 1636 4196 1636
Size: 2560 2119
SfcI C'TryA_G
Cuts at: 33 129 800 1387 1444 1722 2407 3400 3591
Size: 96 671 587 57 278 685 993 191
Cuts at: 3591 33
Size: 1121
Fragments arranged by size:
1121 993 685 671 587 278 191 96
57
SfoI GGC'GCC
Cuts at: 255 2111 2359 255
Size: 1856 248 2575
Fragments arranged by size:
2575 1856 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SgrAI Cr'CCGG_yG
Cuts at: 250 250
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 961 1468 1597 1834 2749 3241 3503 961
Size: 507 129 237 915 492 262 2137
Fragments arranged by size:
2137 915 507 492 262 237 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1090 1732 2232 1090
Size: 642 500 3537
Fragments arranged by size:
3537 642 500
StyI C'CwwG_G
Cuts at: 107 398 1454 1565 1813 2167 107
Size: 291 1056 111 248 354 2619
Fragments arranged by size:
2619 1056 354 291 248 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2262 2395 2854 3063 2262
Size: 133 459 209 3878
Fragments arranged by size:
3878 459 209 133
TatI w'GTAC_w
Cuts at: 1634 4194 1634
Size: 2560 2119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1042 1074 4196 4228 1042
Size: 32 3122 32 1493
Fragments arranged by size:
3122 1493 32 32
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 943 2174 943
Size: 1231 3448
XhoI C'TCGA_G
Cuts at: 1468 1468
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 721 721
Size: 4679
ZraI GAC'GTC
Cuts at: 888 888
Size: 4679
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BglI BmgBI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NotI NruI PacI
PasI PflMI PshAI PsiI PspOMI PsrI SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI