Restriction map for
pF1KB9928
(Circular) (Six-base) MAPSORT of: pF1KB9928.seq Check: 1626 from: 1 to: 4991
pF1KB9928 4991 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2029 2029
Size: 4991
Acc65I G'GTAC_C
Cuts at: 2007 2007
Size: 4991
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 913 1087 1492 1873 2816 3248 3995 4263
Size: 746 174 405 381 943 432 747 268
Cuts at: 4263 167
Size: 895
Fragments arranged by size:
943 895 747 746 432 405 381 268
174
AfeI AGC'GCT
Cuts at: 624 624
Size: 4991
AflIII A'CryG_T
Cuts at: 1568 2343 3447 4424 1568
Size: 775 1104 977 2135
Fragments arranged by size:
2135 1104 977 775
AgeI A'CCGG_T
Cuts at: 3379 3379
Size: 4991
AlwNI CAG_nnn'CTG
Cuts at: 2296 3863 2296
Size: 1567 3424
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 531 909 1995 4344 4600 531
Size: 378 1086 2349 256 922
Fragments arranged by size:
2349 1086 922 378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 1407 2011 1407
Size: 604 4387
BamHI G'GATC_C
Cuts at: 2016 2016
Size: 4991
BanI G'GyrC_C
Cuts at: 1574 2007 2421 2669 2704 1574
Size: 433 414 248 35 3861
Fragments arranged by size:
3861 433 414 248 35
BanII G_rGCy'C
Cuts at: 1805 2005 3035 1805
Size: 200 1030 3761
Fragments arranged by size:
3761 1030 200
BbeI G_GCGC'C
Cuts at: 2425 2673 2425
Size: 248 4743
BbsI GAAGACnn'nnnn_
Cuts at: 395 395
Size: 4991
BbvCI CC'TCA_GC
Cuts at: 1486 1549 1486
Size: 63 4928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 857 891 857
Size: 34 4957
BciVI GTATCCnnnnn_n'
Cuts at: 457 2889 3650 4991 457
Size: 2432 761 1341 457
Fragments arranged by size:
2432 1341 761 457
BclI T'GATC_A
Cuts at: 2511 2511
Size: 4991
BglI GCCn_nnn'nGGC
Cuts at: 1178 1178
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 2103 2103
Size: 4991
Bme1580I G_kGCm'C
Cuts at: 2616 2709 3765 2616
Size: 93 1056 3842
Fragments arranged by size:
3842 1056 93
BmgBI CAC'GTC
Cuts at: 1569 1569
Size: 4991
BmrI ACTGGGnnnn_n'
Cuts at: 1768 1845 2203 2284 2619 4763 1768
Size: 77 358 81 335 2144 1996
Fragments arranged by size:
2144 1996 358 335 81 77
BmtI G_CTAG'C
Cuts at: 623 623
Size: 4991
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3358 3358
Size: 4991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1051 1343 1776 1888 1924 793
Size: 258 292 433 112 36 3860
Fragments arranged by size:
3860 433 292 258 112 36
Bpu10I CC'TnA_GC
Cuts at: 917 1486 1549 917
Size: 569 63 4359
Fragments arranged by size:
4359 569 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 2167 3046 3538 3836 1441
Size: 726 879 492 298 2596
Fragments arranged by size:
2596 879 726 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1258 1342 1880 4695 1258
Size: 84 538 2815 1554
Fragments arranged by size:
2815 1554 538 84
BsaAI yAC'GTr
Cuts at: 410 2344 410
Size: 1934 3057
BsaBI GATnn'nnATC
Cuts at: 6 2505 4542 6
Size: 2499 2037 455
Fragments arranged by size:
2499 2037 455
BsaHI Gr'CG_yC
Cuts at: 2422 2670 3372 4868 2422
Size: 248 702 1496 2545
Fragments arranged by size:
2545 1496 702 248
BsaWI w'CCGG_w
Cuts at: 429 567 2180 2701 3379 3653 3800 4211 429
Size: 138 1613 521 678 274 147 411 1209
Fragments arranged by size:
1613 1209 678 521 411 274 147 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 772 802 1024 1054 1322 1352 1537 1567 772
Size: 30 222 30 268 30 185 30 4196
Fragments arranged by size:
4196 268 222 185 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 1330 1779 246
Size: 1084 449 3458
Fragments arranged by size:
3458 1084 449
BseYI C'CCAG_C
Cuts at: 791 2416 3174 3751 4247 791
Size: 1625 758 577 496 1535
Fragments arranged by size:
1625 1535 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1125 1855 1125
Size: 730 4261
BsiEI CG_ry'CG
Cuts at: 104 1182 2533 2579 3787 104
Size: 1078 1351 46 1208 1308
Fragments arranged by size:
1351 1308 1208 1078 46
BsiHKAI G_wGCw'C
Cuts at: 115 2005 2783 2973 3765 115
Size: 1890 778 190 792 1341
Fragments arranged by size:
1890 1341 792 778 190
BsmI GAATG_Cn'
Cuts at: 163 163
Size: 4991
Bsp1286I G_dGCh'C
Cuts at: 115 1805 2005 2616 2709 2783 2973 3035 3765
Size: 1690 200 611 93 74 190 62 730
Cuts at: 3765 115
Size: 1341
Fragments arranged by size:
1690 1341 730 611 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 429 4211 429
Size: 3782 1209
BspHI T'CATG_A
Cuts at: 4167 4167
Size: 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2041 2255 2557 2938 2041
Size: 214 302 381 4094
Fragments arranged by size:
4094 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3283 3337 4821 4986 50
Size: 3233 54 1484 165 55
Fragments arranged by size:
3233 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 546 1673 2903 546
Size: 1127 1230 2634
Fragments arranged by size:
2634 1230 1127
BsrFI r'CCGG_y
Cuts at: 2989 3170 3379 2989
Size: 181 209 4601
Fragments arranged by size:
4601 209 181
BssSI C'ACGA_G
Cuts at: 3262 3620 3262
Size: 358 4633
BstAPI GCAn_nnn'nTGC
Cuts at: 359 1293 1418 2368 359
Size: 934 125 950 2982
Fragments arranged by size:
2982 950 934 125
BstBI TT'CG_AA
Cuts at: 3352 3352
Size: 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 169 964 1563 169
Size: 795 599 3597
Fragments arranged by size:
3597 795 599
BstYI r'GATC_y
Cuts at: 1 1258 1955 2016 2483 2506 2841 3087 4099
Size: 1257 697 61 467 23 335 246 1012
Cuts at: 4099 4185 4197 4543 1
Size: 86 12 346 449
Fragments arranged by size:
1257 1012 697 467 449 346 335 246
86 61 23 12
BtgI C'CryG_G
Cuts at: 168 168
Size: 4991
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2941 3122 3285 2941
Size: 181 163 4647
Fragments arranged by size:
4647 181 163
BtsI GCAGTG_nn'
Cuts at: 276 558 2234 276
Size: 282 1676 3033
Fragments arranged by size:
3033 1676 282
DraI TTT'AAA
Cuts at: 1990 1990
Size: 4991
DrdI GACnn_nn'nnGTC
Cuts at: 2697 3555 2697
Size: 858 4133
EaeI y'GGCC_r
Cuts at: 1179 1920 2576 2750 3141 3168 4513 1179
Size: 741 656 174 391 27 1345 1657
Fragments arranged by size:
1657 1345 741 656 391 174 27
EagI C'GGCC_G
Cuts at: 1179 2576 1179
Size: 1397 3594
EarI CTCTTCn'nnn_
Cuts at: 1070 3014 3224 1070
Size: 1944 210 2837
Fragments arranged by size:
2837 1944 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1116 3199 3509 3655 4801 1116
Size: 2083 310 146 1146 1306
Fragments arranged by size:
2083 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2003 2003
Size: 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 793 913 1051 1087 1343 1492 1776 1873
Size: 626 120 138 36 256 149 284 97
Cuts at: 1873 1888 1924 2816 3248 3995 4263 167
Size: 15 36 892 432 747 268 895
Fragments arranged by size:
895 892 747 626 432 284 268 256
149 138 120 97 36 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1813 1813
Size: 4991
EcoO109I rG'GnC_Cy
Cuts at: 1461 2130 1461
Size: 669 4322
EcoRI G'AATT_C
Cuts at: 531 1995 531
Size: 1464 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1266 3010 1266
Size: 1744 3247
FspI TGC'GCA
Cuts at: 2772 2772
Size: 4991
HaeII r_GCGC'y
Cuts at: 267 626 2425 2673 3695 4674 267
Size: 359 1799 248 1022 979 584
Fragments arranged by size:
1799 1022 979 584 359 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 222 254 910 942 996 1028 1322 1354 1537
Size: 32 656 32 54 32 294 32 183
Cuts at: 1537 1569 2833 2863 2865 2895 222
Size: 32 1264 30 2 30 2318
Fragments arranged by size:
2318 1264 656 294 183 54 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 2030 1209
Size: 821 4170
KasI G'GCGC_C
Cuts at: 2421 2669 2421
Size: 248 4743
KpnI G_GTAC'C
Cuts at: 2011 2011
Size: 4991
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3662 3846 3662
Size: 184 4807
MscI TGG'CCA
Cuts at: 1922 2752 1922
Size: 830 4161
MslI CAynn'nnrTG
Cuts at: 167 551 1561 1774 2980 3107 167
Size: 384 1010 213 1206 127 2051
Fragments arranged by size:
2051 1206 1010 384 213 127
MspA1I CmG'CkG
Cuts at: 960 2102 2416 2776 3789 4034 960
Size: 1142 314 360 1013 245 1917
Fragments arranged by size:
1917 1142 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3172 3172
Size: 4991
NarI GG'CG_CC
Cuts at: 2422 2670 2422
Size: 248 4743
NcoI C'CATG_G
Cuts at: 168 168
Size: 4991
NgoMIV G'CCGG_C
Cuts at: 3170 3170
Size: 4991
NheI G'CTAG_C
Cuts at: 619 619
Size: 4991
NspI r_CATG'y
Cuts at: 602 2044 2544 3451 602
Size: 1442 500 907 2142
Fragments arranged by size:
2142 1442 907 500
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PfoI T'CCnGG_A
Cuts at: 212 611 1145 212
Size: 399 534 4058
Fragments arranged by size:
4058 534 399
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PmlI CAC'GTG
Cuts at: 410 410
Size: 4991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4959
PpuMI rG'GwC_Cy
Cuts at: 1461 1461
Size: 4991
PsiI TTA'TAA
Cuts at: 653 653
Size: 4991
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 763 795 763
Size: 32 4959
PstI C_TGCA'G
Cuts at: 1298 2038 2723 1298
Size: 740 685 3566
Fragments arranged by size:
3566 740 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 960 2416 2776 960
Size: 1456 360 3175
Fragments arranged by size:
3175 1456 360
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 2005 2005
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 1070 3014 3224 1070
Size: 1944 210 2837
Fragments arranged by size:
2837 1944 210
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 1426 4508 1426
Size: 3082 1909
SfcI C'TryA_G
Cuts at: 33 1294 2034 2719 3712 3903 33
Size: 1261 740 685 993 191 1121
Fragments arranged by size:
1261 1121 993 740 685 191
SfoI GGC'GCC
Cuts at: 2423 2671 2423
Size: 248 4743
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 2013 2013
Size: 4991
SmlI C'TyrA_G
Cuts at: 1456 2146 3061 3553 3815 1456
Size: 690 915 492 262 2632
Fragments arranged by size:
2632 915 690 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 602 2044 2544 602
Size: 1442 500 3049
Fragments arranged by size:
3049 1442 500
StuI AGG'CCT
Cuts at: 1363 1927 1969 1363
Size: 564 42 4385
Fragments arranged by size:
4385 564 42
StyI C'CwwG_G
Cuts at: 168 1373 1394 2125 2479 168
Size: 1205 21 731 354 2680
Fragments arranged by size:
2680 1205 731 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1960 2574 2707 3166 3375 1960
Size: 614 133 459 209 3576
Fragments arranged by size:
3576 614 459 209 133
TatI w'GTAC_w
Cuts at: 1424 4506 1424
Size: 3082 1909
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1024 1056 1558 1590 1885 1917 4508 4540 1024
Size: 32 502 32 295 32 2591 32 1475
Fragments arranged by size:
2591 1475 502 295 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1334 2788 1334
Size: 1454 3537
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 289 1026 2486 289
Size: 737 1460 2794
Fragments arranged by size:
2794 1460 737
XmaI C'CCGG_G
Cuts at: 2011 2011
Size: 4991
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PflMI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI