Restriction map for
pF1KB9921
(Circular) (Six-base) MAPSORT of: pF1KB9921.seq Check: 4994 from: 1 to: 4124
pF1KB9921 4124 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 782 1162 782
Size: 380 3744
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1949 2381 3128 3396 950
Size: 999 432 747 268 1678
Fragments arranged by size:
1678 999 747 432 268
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AleI CACnn'nnGTG
Cuts at: 247 247
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 290 1128 3477 3733 290
Size: 838 2349 256 681
Fragments arranged by size:
2349 838 681 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 117 1144 117
Size: 1027 3097
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 1140 1554 1802 1837 1140
Size: 414 248 35 3427
Fragments arranged by size:
3427 414 248 35
BanII G_rGCy'C
Cuts at: 361 1138 2168 361
Size: 777 1030 2317
Fragments arranged by size:
2317 1030 777
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1336 1417 1752 3896 1336
Size: 81 335 2144 1564
Fragments arranged by size:
2144 1564 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 4124
Bpu10I CC'TnA_GC
Cuts at: 669 669
Size: 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 779 1555 1803 2505 4001 779
Size: 776 248 702 1496 902
Fragments arranged by size:
1496 902 776 702 248
BsaWI w'CCGG_w
Cuts at: 518 1313 1834 2512 2786 2933 3344 518
Size: 795 521 678 274 147 411 1298
Fragments arranged by size:
1298 795 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1549 2307 2884 3380 1549
Size: 758 577 496 2293
Fragments arranged by size:
2293 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 670 1138 1916 2106 2898 670
Size: 468 778 190 792 1896
Fragments arranged by size:
1896 792 778 468 190
Bsp1286I G_dGCh'C
Cuts at: 361 670 1138 1749 1842 1916 2106 2168 2898
Size: 309 468 611 93 74 190 62 730
Cuts at: 2898 361
Size: 1587
Fragments arranged by size:
1587 730 611 468 309 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 917 3300 917
Size: 2383 1741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1174 1388 1690 2071 1174
Size: 214 302 381 3227
Fragments arranged by size:
3227 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 959 2036 959
Size: 1077 3047
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BsrGI T'GTAC_A
Cuts at: 579 579
Size: 4124
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 462 462
Size: 4124
BstYI r'GATC_y
Cuts at: 1 1149 1616 1639 1974 2220 3232 3318 3330
Size: 1148 467 23 335 246 1012 86 12
Cuts at: 3330 3676 1
Size: 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 167 458 1367 167
Size: 291 909 2924
Fragments arranged by size:
2924 909 291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 593 628 593
Size: 35 4089
DraI TTT'AAA
Cuts at: 704 1123 704
Size: 419 3705
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 941 1709 1883 2274 2301 3646 941
Size: 768 174 391 27 1345 1419
Fragments arranged by size:
1419 1345 768 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 1022 2147 2357 1022
Size: 1125 210 2789
Fragments arranged by size:
2789 1125 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 950 1949 2381 3128 3396 587
Size: 363 999 432 747 268 1315
Fragments arranged by size:
1315 999 747 432 363 268
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 647 1558 1806 2828 3807 647
Size: 911 248 1022 979 964
Fragments arranged by size:
1022 979 964 911 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 332 364 1054 1086 1966 1996 1998
Size: 32 187 32 690 32 880 30 2
Cuts at: 1998 2028 113
Size: 30 2209
Fragments arranged by size:
2209 880 690 187 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1101 2795 2979 1101
Size: 1694 184 2246
Fragments arranged by size:
2246 1694 184
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 247 742 2113 2240 247
Size: 495 1371 127 2131
Fragments arranged by size:
2131 1371 495 127
MspA1I CmG'CkG
Cuts at: 940 1235 1549 1909 2922 3167 940
Size: 295 314 360 1013 245 1897
Fragments arranged by size:
1897 1013 360 314 295 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 107 107
Size: 4124
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PasI CC'CwG_GG
Cuts at: 1008 1008
Size: 4124
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PsiI TTA'TAA
Cuts at: 268 268
Size: 4124
PstI C_TGCA'G
Cuts at: 183 936 1171 1856 183
Size: 753 235 685 2451
Fragments arranged by size:
2451 753 685 235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SexAI A'CCwGG_T
Cuts at: 458 458
Size: 4124
SfcI C'TryA_G
Cuts at: 33 179 647 932 1167 1852 2845 3036 33
Size: 146 468 285 235 685 993 191 1121
Fragments arranged by size:
1121 993 685 468 285 235 191 146
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StyI C'CwwG_G
Cuts at: 107 362 1258 1612 107
Size: 255 896 354 2619
Fragments arranged by size:
2619 896 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 717 1707 1840 2299 2508 717
Size: 990 133 459 209 2333
Fragments arranged by size:
2333 990 459 209 133
TatI w'GTAC_w
Cuts at: 487 579 902 3639 487
Size: 92 323 2737 972
Fragments arranged by size:
2737 972 323 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 127 1921 127
Size: 1794 2330
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI