Restriction map for
pF1KB9919
(Circular) (Six-base) MAPSORT of: pF1KB9919.seq Check: 4727 from: 1 to: 4025
pF1KB9919 4025 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 633 633
Size: 4025
AccI GT'mk_AC
Cuts at: 962 974 1063 962
Size: 12 89 3924
Fragments arranged by size:
3924 89 12
Acc65I G'GTAC_C
Cuts at: 1041 1041
Size: 4025
AclI AA'CG_TT
Cuts at: 3865 3865
Size: 4025
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1850 2282 3029 3297 772
Size: 1078 432 747 268 1500
Fragments arranged by size:
1500 1078 747 432 268
AflIII A'CryG_T
Cuts at: 1377 2481 3458 1377
Size: 1104 977 1944
Fragments arranged by size:
1944 1104 977
AgeI A'CCGG_T
Cuts at: 2413 2413
Size: 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 315 483 315
Size: 168 3857
AlwNI CAG_nnn'CTG
Cuts at: 322 682 1330 2897 322
Size: 360 648 1567 1450
Fragments arranged by size:
1567 1450 648 360
ApaLI G'TGCA_C
Cuts at: 2795 2795
Size: 4025
ApoI r'AATT_y
Cuts at: 375 402 1029 3378 3634 375
Size: 27 627 2349 256 766
Fragments arranged by size:
2349 766 627 256 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4025
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
AvaI C'yCGr_G
Cuts at: 1045 1045
Size: 4025
BamHI G'GATC_C
Cuts at: 1050 1050
Size: 4025
BanI G'GyrC_C
Cuts at: 1041 1455 1703 1738 1041
Size: 414 248 35 3328
Fragments arranged by size:
3328 414 248 35
BanII G_rGCy'C
Cuts at: 1039 2069 1039
Size: 1030 2995
BbeI G_GCGC'C
Cuts at: 1459 1707 1459
Size: 248 3777
BciVI GTATCCnnnnn_n'
Cuts at: 1923 2684 4025 1923
Size: 761 1341 1923
Fragments arranged by size:
1923 1341 761
BclI T'GATC_A
Cuts at: 608 1545 608
Size: 937 3088
BglII A'GATC_T
Cuts at: 1540 3577 1540
Size: 2037 1988
BlpI GC'TnA_GC
Cuts at: 1137 1137
Size: 4025
Bme1580I G_kGCm'C
Cuts at: 316 1650 1743 2799 316
Size: 1334 93 1056 1542
Fragments arranged by size:
1542 1334 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 505 1237 1318 1653 3797 505
Size: 732 81 335 2144 733
Fragments arranged by size:
2144 733 732 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 4025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 541 659 977 262
Size: 279 118 318 3310
Fragments arranged by size:
3310 318 279 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1201 2080 2572 2870 865
Size: 336 879 492 298 2020
Fragments arranged by size:
2020 879 492 336 298
BsaI GGTCTCn'nnnn_
Cuts at: 3729 3729
Size: 4025
BsaAI yAC'GTr
Cuts at: 1378 1378
Size: 4025
BsaBI GATnn'nnATC
Cuts at: 6 1539 3576 6
Size: 1533 2037 455
Fragments arranged by size:
2037 1533 455
BsaHI Gr'CG_yC
Cuts at: 1456 1704 2406 3902 1456
Size: 248 702 1496 1579
Fragments arranged by size:
1579 1496 702 248
BsaWI w'CCGG_w
Cuts at: 768 1214 1735 2413 2687 2834 3245 768
Size: 446 521 678 274 147 411 1548
Fragments arranged by size:
1548 678 521 446 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 657 687 657
Size: 30 3995
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 609 385
Size: 224 3801
BseYI C'CCAG_C
Cuts at: 309 707 1450 2208 2785 3281 309
Size: 398 743 758 577 496 1053
Fragments arranged by size:
1053 758 743 577 496 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 232
Size: 4025
BsiEI CG_ry'CG
Cuts at: 104 1567 1613 2821 104
Size: 1463 46 1208 1308
Fragments arranged by size:
1463 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 506 1039 1817 2007 2799 506
Size: 533 778 190 792 1732
Fragments arranged by size:
1732 792 778 533 190
Bsp1286I G_dGCh'C
Cuts at: 316 506 1039 1650 1743 1817 2007 2069 2799
Size: 190 533 611 93 74 190 62 730
Cuts at: 2799 316
Size: 1542
Fragments arranged by size:
1542 730 611 533 190 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3245 3245
Size: 4025
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 633 1075 1289 1591 1972 633
Size: 442 214 302 381 2686
Fragments arranged by size:
2686 442 381 302 214
BsrBI CCG'CTC
Cuts at: 50 210 280 2317 2371 3855 4020 50
Size: 160 70 2037 54 1484 165 55
Fragments arranged by size:
2037 1484 165 160 70 55 54
BsrDI GCAATG_nn'
Cuts at: 1937 1937
Size: 4025
BsrFI r'CCGG_y
Cuts at: 2023 2204 2413 2023
Size: 181 209 3635
Fragments arranged by size:
3635 209 181
BssSI C'ACGA_G
Cuts at: 2296 2654 2296
Size: 358 3667
BstAPI GCAn_nnn'nTGC
Cuts at: 692 1402 692
Size: 710 3315
BstBI TT'CG_AA
Cuts at: 2386 2386
Size: 4025
BstYI r'GATC_y
Cuts at: 1 1050 1517 1540 1875 2121 3133 3219 3231
Size: 1049 467 23 335 246 1012 86 12
Cuts at: 3231 3577 1
Size: 346 449
Fragments arranged by size:
1049 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 930 930
Size: 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 990 1975 2156 2319 990
Size: 985 181 163 2696
Fragments arranged by size:
2696 985 181 163
BtsI GCAGTG_nn'
Cuts at: 465 1268 465
Size: 803 3222
DraI TTT'AAA
Cuts at: 1024 1024
Size: 4025
DraIII CAC_nnn'GTG
Cuts at: 692 692
Size: 4025
DrdI GACnn_nn'nnGTC
Cuts at: 1731 2589 1731
Size: 858 3167
EaeI y'GGCC_r
Cuts at: 142 548 1610 1784 2175 2202 3547 142
Size: 406 1062 174 391 27 1345 620
Fragments arranged by size:
1345 1062 620 406 391 174 27
EagI C'GGCC_G
Cuts at: 1610 1610
Size: 4025
EarI CTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 550 2233 2543 2689 3835 550
Size: 1683 310 146 1146 740
Fragments arranged by size:
1683 1146 740 310 146
EcoICRI GAG'CTC
Cuts at: 1037 1037
Size: 4025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 541 659 772 977 1850 2282 3029 3297
Size: 279 118 113 205 873 432 747 268
Cuts at: 3297 262
Size: 990
Fragments arranged by size:
990 873 747 432 279 268 205 118
113
EcoO109I rG'GnC_Cy
Cuts at: 1164 1164
Size: 4025
EcoRI G'AATT_C
Cuts at: 375 402 1029 375
Size: 27 627 3371
Fragments arranged by size:
3371 627 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 4025
FspI TGC'GCA
Cuts at: 1806 1806
Size: 4025
HaeII r_GCGC'y
Cuts at: 310 1459 1707 2729 3708 310
Size: 1149 248 1022 979 627
Fragments arranged by size:
1149 1022 979 627 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1867 1897 1899 1929 1867
Size: 30 2 30 3963
Fragments arranged by size:
3963 30 30 2
HincII GTy'rAC
Cuts at: 1064 1064
Size: 4025
KasI G'GCGC_C
Cuts at: 1455 1703 1455
Size: 248 3777
KpnI G_GTAC'C
Cuts at: 1045 1045
Size: 4025
MluI A'CGCG_T
Cuts at: 3458 3458
Size: 4025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2696 2880 2696
Size: 184 3841
MscI TGG'CCA
Cuts at: 1786 1786
Size: 4025
MslI CAynn'nnrTG
Cuts at: 487 935 2014 2141 487
Size: 448 1079 127 2371
Fragments arranged by size:
2371 1079 448 127
MspA1I CmG'CkG
Cuts at: 225 679 1136 1450 1810 2823 3068 225
Size: 454 457 314 360 1013 245 1182
Fragments arranged by size:
1182 1013 457 454 360 314 245
NaeI GCC'GGC
Cuts at: 2206 2206
Size: 4025
NarI GG'CG_CC
Cuts at: 1456 1704 1456
Size: 248 3777
NcoI C'CATG_G
Cuts at: 930 930
Size: 4025
NgoMIV G'CCGG_C
Cuts at: 2204 2204
Size: 4025
NspI r_CATG'y
Cuts at: 828 1078 1578 2485 828
Size: 250 500 907 2368
Fragments arranged by size:
2368 907 500 250
PciI A'CATG_T
Cuts at: 2481 2481
Size: 4025
PflMI CCAn_nnn'nTGG
Cuts at: 682 682
Size: 4025
PfoI T'CCnGG_A
Cuts at: 518 518
Size: 4025
PmeI GTTT'AAAC
Cuts at: 1024 1024
Size: 4025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3993
PstI C_TGCA'G
Cuts at: 1072 1757 1072
Size: 685 3340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4025
PvuII CAG'CTG
Cuts at: 679 1450 1810 679
Size: 771 360 2894
Fragments arranged by size:
2894 771 360
RsrII CG'GwC_CG
Cuts at: 2220 2220
Size: 4025
SacI G_AGCT'C
Cuts at: 1039 1039
Size: 4025
SalI G'TCGA_C
Cuts at: 1062 1062
Size: 4025
SapI GCTCTTCn'nnn_
Cuts at: 2048 2258 2048
Size: 210 3815
SbfI CC_TGCA'GG
Cuts at: 1072 1072
Size: 4025
ScaI AGT'ACT
Cuts at: 3542 3542
Size: 4025
SexAI A'CCwGG_T
Cuts at: 389 389
Size: 4025
SfcI C'TryA_G
Cuts at: 33 1068 1753 2746 2937 33
Size: 1035 685 993 191 1121
Fragments arranged by size:
1121 1035 993 685 191
SfoI GGC'GCC
Cuts at: 1457 1705 1457
Size: 248 3777
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4025
SmaI CCC'GGG
Cuts at: 1047 1047
Size: 4025
SmlI C'TyrA_G
Cuts at: 844 1180 2095 2587 2849 844
Size: 336 915 492 262 2020
Fragments arranged by size:
2020 915 492 336 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4025
SphI G_CATG'C
Cuts at: 1078 1578 1078
Size: 500 3525
StuI AGG'CCT
Cuts at: 195 954 195
Size: 759 3266
StyI C'CwwG_G
Cuts at: 909 930 968 1159 1513 909
Size: 21 38 191 354 3421
Fragments arranged by size:
3421 354 191 38 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 250 438 1608 1741 2200 2409 250
Size: 188 1170 133 459 209 1866
Fragments arranged by size:
1866 1170 459 209 188 133
TatI w'GTAC_w
Cuts at: 3540 3540
Size: 4025
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 657 689 3542 3574 657
Size: 32 2853 32 1108
Fragments arranged by size:
2853 1108 32 32
Tth111I GACn'n_nGTC
Cuts at: 1822 1822
Size: 4025
XbaI T'CTAG_A
Cuts at: 1056 1056
Size: 4025
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 421 874 1520 421
Size: 453 646 2926
Fragments arranged by size:
2926 646 453
XmaI C'CCGG_G
Cuts at: 1045 1045
Size: 4025
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI