Restriction map for
pF1KB9918
(Circular) (Six-base) MAPSORT of: pF1KB9918.seq Check: 269 from: 1 to: 4850
pF1KB9918 4850 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 743 743
Size: 4850
AccI GT'mk_AC
Cuts at: 112 1888 112
Size: 1776 3074
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 722 729 1826 2675 3107 3854 4122 449
Size: 273 7 1097 849 432 747 268 1177
Fragments arranged by size:
1177 1097 849 747 432 273 268 7
AfeI AGC'GCT
Cuts at: 560 1147 560
Size: 587 4263
AflIII A'CryG_T
Cuts at: 778 1280 2202 3306 4283 778
Size: 502 922 1104 977 1345
Fragments arranged by size:
1345 1104 977 922 502
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1139 1139
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 121 1375 2155 3722 121
Size: 1254 780 1567 1249
Fragments arranged by size:
1567 1254 1249 780
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 623 867 1854 4203 4459 623
Size: 244 987 2349 256 1014
Fragments arranged by size:
2349 1014 987 256 244
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 1870 1870
Size: 4850
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 696 1866 2280 2528 2563 696
Size: 1170 414 248 35 2983
Fragments arranged by size:
2983 1170 414 248 35
BanII G_rGCy'C
Cuts at: 439 1864 2894 439
Size: 1425 1030 2395
Fragments arranged by size:
2395 1425 1030
BbeI G_GCGC'C
Cuts at: 2284 2532 2284
Size: 248 4602
BbsI GAAGACnn'nnnn_
Cuts at: 137 735 137
Size: 598 4252
BciVI GTATCCnnnnn_n'
Cuts at: 1016 2748 3509 4850 1016
Size: 1732 761 1341 1016
Fragments arranged by size:
1732 1341 1016 761
BclI T'GATC_A
Cuts at: 2370 2370
Size: 4850
BglII A'GATC_T
Cuts at: 2365 4402 2365
Size: 2037 2813
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 1567 2475 2568 3624 1567
Size: 908 93 1056 2793
Fragments arranged by size:
2793 1056 908 93
BmrI ACTGGGnnnn_n'
Cuts at: 1400 2062 2143 2478 4622 1400
Size: 662 81 335 2144 1628
Fragments arranged by size:
2144 1628 662 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 4850
Bpu10I CC'TnA_GC
Cuts at: 875 875
Size: 4850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 2905 3397 3695 2026
Size: 879 492 298 3181
Fragments arranged by size:
3181 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4554 4554
Size: 4850
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 740 2281 2529 3231 4727 740
Size: 1541 248 702 1496 863
Fragments arranged by size:
1541 1496 863 702 248
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3512 3659 4070 2039
Size: 521 678 274 147 411 2819
Fragments arranged by size:
2819 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 123 126 129 254 120
Size: 3 3 3 125 4716
Fragments arranged by size:
4716 125 3 3 3
BseYI C'CCAG_C
Cuts at: 749 810 923 2275 3033 3610 4106 749
Size: 61 113 1352 758 577 496 1493
Fragments arranged by size:
1493 1352 758 577 496 113 61
BsiEI CG_ry'CG
Cuts at: 104 152 2392 2438 3646 104
Size: 48 2240 46 1208 1308
Fragments arranged by size:
2240 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 1864 2642 2832 3624 1864
Size: 778 190 792 3090
Fragments arranged by size:
3090 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 439 1567 1864 2475 2568 2642 2832 2894 3624
Size: 1128 297 611 93 74 190 62 730
Cuts at: 3624 439
Size: 1665
Fragments arranged by size:
1665 1128 730 611 297 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 4026 4026
Size: 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1900 2114 2416 2797 1900
Size: 214 302 381 3953
Fragments arranged by size:
3953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3142 3196 4680 4845 50
Size: 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 469 503 1070 1792 2762 469
Size: 34 567 722 970 2557
Fragments arranged by size:
2557 970 722 567 34
BsrFI r'CCGG_y
Cuts at: 151 2848 3029 3238 151
Size: 2697 181 209 1763
Fragments arranged by size:
2697 1763 209 181
BssSI C'ACGA_G
Cuts at: 3121 3479 3121
Size: 358 4492
BstAPI GCAn_nnn'nTGC
Cuts at: 2227 2227
Size: 4850
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstXI CCAn_nnnn'nTGG
Cuts at: 1518 1518
Size: 4850
BstYI r'GATC_y
Cuts at: 1 915 1339 1875 2342 2365 2700 2946 3958
Size: 914 424 536 467 23 335 246 1012
Cuts at: 3958 4044 4056 4402 1
Size: 86 12 346 449
Fragments arranged by size:
1012 914 536 467 449 424 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1428 1428
Size: 4850
BtgI C'CryG_G
Cuts at: 1221 1465 1221
Size: 244 4606
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 1236 1595 2093 1236
Size: 359 498 3993
Fragments arranged by size:
3993 498 359
DraI TTT'AAA
Cuts at: 1849 1849
Size: 4850
DrdI GACnn_nn'nnGTC
Cuts at: 2556 3414 2556
Size: 858 3992
EaeI y'GGCC_r
Cuts at: 149 2435 2609 3000 3027 4372 149
Size: 2286 174 391 27 1345 627
Fragments arranged by size:
2286 1345 627 391 174 27
EagI C'GGCC_G
Cuts at: 149 2435 149
Size: 2286 2564
EarI CTCTTCn'nnn_
Cuts at: 188 218 231 2873 3083 188
Size: 30 13 2642 210 1955
Fragments arranged by size:
2642 1955 210 30 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3368 3514 4660 3058
Size: 310 146 1146 3248
Fragments arranged by size:
3248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1862 1862
Size: 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 722 729 1826 2675 3107 3854 4122 449
Size: 273 7 1097 849 432 747 268 1177
Fragments arranged by size:
1177 1097 849 747 432 273 268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 1729 1729
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 180 573 1989 180
Size: 393 1416 3041
Fragments arranged by size:
3041 1416 393
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4850
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 562 930 1149 2284 2532 3554 4533 562
Size: 368 219 1135 248 1022 979 879
Fragments arranged by size:
1135 1022 979 879 368 248 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 765 797 2692 2722 2724 2754 765
Size: 32 1895 30 2 30 2861
Fragments arranged by size:
2861 1895 32 30 30 2
HincII GTy'rAC
Cuts at: 1889 1889
Size: 4850
HindIII A'AGCT_T
Cuts at: 379 1206 1744 379
Size: 827 538 3485
Fragments arranged by size:
3485 827 538
KasI G'GCGC_C
Cuts at: 2280 2528 2280
Size: 248 4602
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 778 4283 778
Size: 3505 1345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 213 763 3521 3705 213
Size: 550 2758 184 1358
Fragments arranged by size:
2758 1358 550 184
MscI TGG'CCA
Cuts at: 2611 2611
Size: 4850
MslI CAynn'nnrTG
Cuts at: 1108 1516 2839 2966 1108
Size: 408 1323 127 2992
Fragments arranged by size:
2992 1323 408 127
MspA1I CmG'CkG
Cuts at: 749 1961 2275 2635 3648 3893 749
Size: 1212 314 360 1013 245 1706
Fragments arranged by size:
1706 1212 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 2281 2529 2281
Size: 248 4602
NcoI C'CATG_G
Cuts at: 1465 1465
Size: 4850
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NspI r_CATG'y
Cuts at: 549 1284 1903 2403 3310 549
Size: 735 619 500 907 2089
Fragments arranged by size:
2089 907 735 619 500
PciI A'CATG_T
Cuts at: 1280 3306 1280
Size: 2026 2824
PflMI CCAn_nnn'nTGG
Cuts at: 528 528
Size: 4850
PfoI T'CCnGG_A
Cuts at: 981 981
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4818
PpuMI rG'GwC_Cy
Cuts at: 180 573 180
Size: 393 4457
PstI C_TGCA'G
Cuts at: 1897 2582 1897
Size: 685 4165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 749 2275 2635 749
Size: 1526 360 2964
Fragments arranged by size:
2964 1526 360
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1864 1864
Size: 4850
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 2873 3083 2873
Size: 210 4640
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SexAI A'CCwGG_T
Cuts at: 1374 1374
Size: 4850
SfcI C'TryA_G
Cuts at: 33 113 1546 1893 2578 3571 3762 33
Size: 80 1433 347 685 993 191 1121
Fragments arranged by size:
1433 1121 993 685 347 191 80
SfoI GGC'GCC
Cuts at: 2282 2530 2282
Size: 248 4602
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 2005 2920 3412 3674 2005
Size: 915 492 262 3181
Fragments arranged by size:
3181 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1674 67
Size: 1607 3243
SphI G_CATG'C
Cuts at: 549 1903 2403 549
Size: 1354 500 2996
Fragments arranged by size:
2996 1354 500
SspI AAT'ATT
Cuts at: 372 372
Size: 4850
StyI C'CwwG_G
Cuts at: 522 1465 1984 2338 522
Size: 943 519 354 3034
Fragments arranged by size:
3034 943 519 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2433 2566 3025 3234 2433
Size: 133 459 209 4049
Fragments arranged by size:
4049 459 209 133
TatI w'GTAC_w
Cuts at: 1051 4365 1051
Size: 3314 1536
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4367 4399 4367
Size: 32 4818
Tth111I GACn'n_nGTC
Cuts at: 2647 2647
Size: 4850
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1581 2345 1581
Size: 764 4086
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
ZraI GAC'GTC
Cuts at: 741 741
Size: 4850
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI