Restriction map for
pF1KB9913
(Circular) (Six-base) MAPSORT of: pF1KB9913.seq Check: 5160 from: 1 to: 3968
pF1KB9913 3968 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 911 1006 911
Size: 95 3873
Acc65I G'GTAC_C
Cuts at: 984 984
Size: 3968
AclI AA'CG_TT
Cuts at: 3808 3808
Size: 3968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1793 2225 2972 3240 920
Size: 873 432 747 268 1648
Fragments arranged by size:
1648 873 747 432 268
AflIII A'CryG_T
Cuts at: 1320 2424 3401 1320
Size: 1104 977 1887
Fragments arranged by size:
1887 1104 977
AgeI A'CCGG_T
Cuts at: 2356 2356
Size: 3968
AleI CACnn'nnGTG
Cuts at: 901 901
Size: 3968
AlwNI CAG_nnn'CTG
Cuts at: 1273 2840 1273
Size: 1567 2401
ApaLI G'TGCA_C
Cuts at: 882 2738 882
Size: 1856 2112
ApoI r'AATT_y
Cuts at: 286 315 972 3321 3577 286
Size: 29 657 2349 256 677
Fragments arranged by size:
2349 677 657 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 3968
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
AvaI C'yCGr_G
Cuts at: 988 988
Size: 3968
BamHI G'GATC_C
Cuts at: 993 993
Size: 3968
BanI G'GyrC_C
Cuts at: 984 1398 1646 1681 984
Size: 414 248 35 3271
Fragments arranged by size:
3271 414 248 35
BanII G_rGCy'C
Cuts at: 982 2012 982
Size: 1030 2938
BbeI G_GCGC'C
Cuts at: 1402 1650 1402
Size: 248 3720
BbsI GAAGACnn'nnnn_
Cuts at: 455 455
Size: 3968
BciVI GTATCCnnnnn_n'
Cuts at: 1866 2627 3968 1866
Size: 761 1341 1866
Fragments arranged by size:
1866 1341 761
BclI T'GATC_A
Cuts at: 1488 1488
Size: 3968
BglII A'GATC_T
Cuts at: 1483 3520 1483
Size: 2037 1931
BlpI GC'TnA_GC
Cuts at: 1080 1080
Size: 3968
Bme1580I G_kGCm'C
Cuts at: 886 1593 1686 2742 886
Size: 707 93 1056 2112
Fragments arranged by size:
2112 1056 707 93
BmgBI CAC'GTC
Cuts at: 607 607
Size: 3968
BmrI ACTGGGnnnn_n'
Cuts at: 868 1180 1261 1596 3740 868
Size: 312 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 312 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2335 2335
Size: 3968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2023 2515 2813 1144
Size: 879 492 298 2299
Fragments arranged by size:
2299 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3672 3672
Size: 3968
BsaAI yAC'GTr
Cuts at: 1321 1321
Size: 3968
BsaBI GATnn'nnATC
Cuts at: 6 1482 3519 6
Size: 1476 2037 455
Fragments arranged by size:
2037 1476 455
BsaHI Gr'CG_yC
Cuts at: 1399 1647 2349 3845 1399
Size: 248 702 1496 1522
Fragments arranged by size:
1522 1496 702 248
BsaWI w'CCGG_w
Cuts at: 120 256 1157 1678 2356 2630 2777 3188 120
Size: 136 901 521 678 274 147 411 900
Fragments arranged by size:
901 900 678 521 411 274 147 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 328
Size: 30 3938
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 766 178
Size: 588 3380
BseYI C'CCAG_C
Cuts at: 1393 2151 2728 3224 1393
Size: 758 577 496 2137
Fragments arranged by size:
2137 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1510 1556 2764 104
Size: 1406 46 1208 1308
Fragments arranged by size:
1406 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 886 982 1760 1950 2742 886
Size: 96 778 190 792 2112
Fragments arranged by size:
2112 792 778 190 96
Bsp1286I G_dGCh'C
Cuts at: 886 982 1593 1686 1760 1950 2012 2742 886
Size: 96 611 93 74 190 62 730 2112
Fragments arranged by size:
2112 730 611 190 96 93 74 62
BspEI T'CCGG_A
Cuts at: 120 3188 120
Size: 3068 900
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 3968
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1018 1232 1534 1915 1018
Size: 214 302 381 3071
Fragments arranged by size:
3071 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2260 2314 3798 3963 50
Size: 2210 54 1484 165 55
Fragments arranged by size:
2210 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 725 1880 725
Size: 1155 2813
BsrFI r'CCGG_y
Cuts at: 113 168 1966 2147 2356 113
Size: 55 1798 181 209 1725
Fragments arranged by size:
1798 1725 209 181 55
BssSI C'ACGA_G
Cuts at: 2239 2597 2239
Size: 358 3610
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 1345
Size: 3968
BstBI TT'CG_AA
Cuts at: 2329 2329
Size: 3968
BstEII G'GTnAC_C
Cuts at: 465 577 465
Size: 112 3856
BstXI CCAn_nnnn'nTGG
Cuts at: 903 903
Size: 3968
BstYI r'GATC_y
Cuts at: 1 364 993 1460 1483 1818 2064 3076 3162
Size: 363 629 467 23 335 246 1012 86
Cuts at: 3162 3174 3520 1
Size: 12 346 449
Fragments arranged by size:
1012 629 467 449 363 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 372 372
Size: 3968
BtgI C'CryG_G
Cuts at: 582 582
Size: 3968
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1918 2099 2262 1918
Size: 181 163 3624
Fragments arranged by size:
3624 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1211
Size: 3968
DraI TTT'AAA
Cuts at: 967 967
Size: 3968
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2532 1674
Size: 858 3110
EaeI y'GGCC_r
Cuts at: 1553 1727 2118 2145 3490 1553
Size: 174 391 27 1345 2031
Fragments arranged by size:
2031 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1553 1553
Size: 3968
EarI CTCTTCn'nnn_
Cuts at: 143 1991 2201 143
Size: 1848 210 1910
Fragments arranged by size:
1910 1848 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2176 2486 2632 3778 2176
Size: 310 146 1146 2366
Fragments arranged by size:
2366 1146 310 146
EcoICRI GAG'CTC
Cuts at: 980 980
Size: 3968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1793 2225 2972 3240 920
Size: 873 432 747 268 1648
Fragments arranged by size:
1648 873 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1107 1107
Size: 3968
EcoRI G'AATT_C
Cuts at: 972 972
Size: 3968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3968
FspI TGC'GCA
Cuts at: 1749 1749
Size: 3968
HaeII r_GCGC'y
Cuts at: 1402 1650 2672 3651 1402
Size: 248 1022 979 1719
Fragments arranged by size:
1719 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 591 623 1810 1840 1842 1872 331
Size: 32 228 32 1187 30 2 30 2427
Fragments arranged by size:
2427 1187 228 32 32 30 30 2
HincII GTy'rAC
Cuts at: 610 1007 610
Size: 397 3571
KasI G'GCGC_C
Cuts at: 1398 1646 1398
Size: 248 3720
KpnI G_GTAC'C
Cuts at: 988 988
Size: 3968
MluI A'CGCG_T
Cuts at: 3401 3401
Size: 3968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 526 2639 2823 424
Size: 102 2113 184 1569
Fragments arranged by size:
2113 1569 184 102
MscI TGG'CCA
Cuts at: 1729 1729
Size: 3968
MslI CAynn'nnrTG
Cuts at: 901 1957 2084 901
Size: 1056 127 2785
Fragments arranged by size:
2785 1056 127
MspA1I CmG'CkG
Cuts at: 1079 1393 1753 2766 3011 1079
Size: 314 360 1013 245 2036
Fragments arranged by size:
2036 1013 360 314 245
NaeI GCC'GGC
Cuts at: 170 2149 170
Size: 1979 1989
NarI GG'CG_CC
Cuts at: 1399 1647 1399
Size: 248 3720
NcoI C'CATG_G
Cuts at: 582 582
Size: 3968
NgoMIV G'CCGG_C
Cuts at: 168 2147 168
Size: 1979 1989
NspI r_CATG'y
Cuts at: 1021 1521 2428 1021
Size: 500 907 2561
Fragments arranged by size:
2561 907 500
PciI A'CATG_T
Cuts at: 2424 2424
Size: 3968
PflMI CCAn_nnn'nTGG
Cuts at: 588 588
Size: 3968
PmeI GTTT'AAAC
Cuts at: 967 967
Size: 3968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 3107
Size: 32 3936
PstI C_TGCA'G
Cuts at: 1015 1700 1015
Size: 685 3283
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3968
PvuII CAG'CTG
Cuts at: 1393 1753 1393
Size: 360 3608
RsrII CG'GwC_CG
Cuts at: 2163 2163
Size: 3968
SacI G_AGCT'C
Cuts at: 982 982
Size: 3968
SalI G'TCGA_C
Cuts at: 1005 1005
Size: 3968
SapI GCTCTTCn'nnn_
Cuts at: 143 1991 2201 143
Size: 1848 210 1910
Fragments arranged by size:
1910 1848 210
SbfI CC_TGCA'GG
Cuts at: 1015 1015
Size: 3968
ScaI AGT'ACT
Cuts at: 3485 3485
Size: 3968
SfcI C'TryA_G
Cuts at: 33 1011 1696 2689 2880 33
Size: 978 685 993 191 1121
Fragments arranged by size:
1121 993 978 685 191
SfoI GGC'GCC
Cuts at: 1400 1648 1400
Size: 248 3720
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3968
SmaI CCC'GGG
Cuts at: 990 990
Size: 3968
SmlI C'TyrA_G
Cuts at: 1123 2038 2530 2792 1123
Size: 915 492 262 2299
Fragments arranged by size:
2299 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3968
SphI G_CATG'C
Cuts at: 1021 1521 1021
Size: 500 3468
StuI AGG'CCT
Cuts at: 553 553
Size: 3968
StyI C'CwwG_G
Cuts at: 582 1102 1456 582
Size: 520 354 3094
Fragments arranged by size:
3094 520 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 887 1551 1684 2143 2352 887
Size: 664 133 459 209 2503
Fragments arranged by size:
2503 664 459 209 133
TatI w'GTAC_w
Cuts at: 459 3483 459
Size: 3024 944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 3936
Tth111I GACn'n_nGTC
Cuts at: 1765 1765
Size: 3968
XbaI T'CTAG_A
Cuts at: 999 999
Size: 3968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1463 330
Size: 1133 2835
XmaI C'CCGG_G
Cuts at: 988 988
Size: 3968
XmnI GAAnn'nnTTC
Cuts at: 929 929
Size: 3968
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI