Restriction map for
pF1KB9900
(Circular) (Six-base) MAPSORT of: pF1KB9900.seq Check: 5879 from: 1 to: 4235
pF1KB9900 4235 bp
With 182 enzymes: SgfI *
July 3, 2012 16:39 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1058 1193 1251 1058
Size: 135 58 4042
Fragments arranged by size:
4042 135 58
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1122 2060 2492 3239 3507 1122
Size: 938 432 747 268 1850
Fragments arranged by size:
1850 938 747 432 268
AfeI AGC'GCT
Cuts at: 538 538
Size: 4235
AflIII A'CryG_T
Cuts at: 806 1587 2691 3668 806
Size: 781 1104 977 1373
Fragments arranged by size:
1373 1104 977 781
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AleI CACnn'nnGTG
Cuts at: 370 370
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 141 180 572 783 1071 1540 3107 141
Size: 39 392 211 288 469 1567 1269
Fragments arranged by size:
1567 1269 469 392 288 211 39
ApaI G_GGCC'C
Cuts at: 124 973 124
Size: 849 3386
ApaLI G'TGCA_C
Cuts at: 469 3005 469
Size: 2536 1699
ApoI r'AATT_y
Cuts at: 1239 3588 3844 1239
Size: 2349 256 1630
Fragments arranged by size:
2349 1630 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 218 1255 218
Size: 1037 3198
AvrII C'CTAG_G
Cuts at: 241 241
Size: 4235
BamHI G'GATC_C
Cuts at: 111 235 1260 111
Size: 124 1025 3086
Fragments arranged by size:
3086 1025 124
BanI G'GyrC_C
Cuts at: 146 399 578 1058 1193 1251 1665 1913 1948
Size: 253 179 480 135 58 414 248 35
Cuts at: 1948 146
Size: 2433
Fragments arranged by size:
2433 480 414 253 248 179 135 58
35
BanII G_rGCy'C
Cuts at: 124 282 973 1042 1249 2279 124
Size: 158 691 69 207 1030 2080
Fragments arranged by size:
2080 1030 691 207 158 69
BbeI G_GCGC'C
Cuts at: 150 1669 1917 150
Size: 1519 248 2468
Fragments arranged by size:
2468 1519 248
BciVI GTATCCnnnnn_n'
Cuts at: 592 2133 2894 4235 592
Size: 1541 761 1341 592
Fragments arranged by size:
1541 1341 761 592
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglI GCCn_nnn'nGGC
Cuts at: 138 531 943 138
Size: 393 412 3430
Fragments arranged by size:
3430 412 393
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 124 404 473 959 973 1149 1860 1953 3009
Size: 280 69 486 14 176 711 93 1056
Cuts at: 3009 124
Size: 1350
Fragments arranged by size:
1350 1056 711 486 280 176 93 69
14
BmgBI CAC'GTC
Cuts at: 353 807 353
Size: 454 3781
BmrI ACTGGGnnnn_n'
Cuts at: 486 491 1447 1528 1863 4007 486
Size: 5 956 81 335 2144 714
Fragments arranged by size:
2144 956 714 335 81 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 682 961 420
Size: 262 279 3694
Fragments arranged by size:
3694 279 262
Bpu10I CC'TnA_GC
Cuts at: 1096 1096
Size: 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1411 2290 2782 3080 781
Size: 630 879 492 298 1936
Fragments arranged by size:
1936 879 630 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1023 1588 1023
Size: 565 3670
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 147 1010 1666 1914 2616 4112 147
Size: 863 656 248 702 1496 270
Fragments arranged by size:
1496 863 702 656 270 248
BsaWI w'CCGG_w
Cuts at: 1424 1945 2623 2897 3044 3455 1424
Size: 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 264 294 655 685 802 832 844 874 264
Size: 30 361 30 117 30 12 30 3625
Fragments arranged by size:
3625 361 117 30 30 30 30 12
BseYI C'CCAG_C
Cuts at: 565 966 973 1142 1660 2418 2995 3491 565
Size: 401 7 169 518 758 577 496 1309
Fragments arranged by size:
1309 758 577 518 496 401 169 7
BsiEI CG_ry'CG
Cuts at: 104 413 1777 1823 3031 104
Size: 309 1364 46 1208 1308
Fragments arranged by size:
1364 1308 1208 309 46
BsiHKAI G_wGCw'C
Cuts at: 473 1249 2027 2217 3009 473
Size: 776 778 190 792 1699
Fragments arranged by size:
1699 792 778 776 190
BsmBI CGTCTCn'nnnn_
Cuts at: 215 847 1111 215
Size: 632 264 3339
Fragments arranged by size:
3339 632 264
Bsp1286I G_dGCh'C
Cuts at: 124 282 404 473 959 973 1042 1149 1249
Size: 158 122 69 486 14 69 107 100
Cuts at: 1249 1860 1953 2027 2217 2279 3009 124
Size: 611 93 74 190 62 730 1350
Fragments arranged by size:
1350 730 611 486 190 158 122 107
100 93 74 69 69 62 14
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 1070 1285 1499 1801 2182 215
Size: 855 215 214 302 381 2268
Fragments arranged by size:
2268 855 381 302 215 214
BsrBI CCG'CTC
Cuts at: 50 696 2527 2581 4065 4230 50
Size: 646 1831 54 1484 165 55
Fragments arranged by size:
1831 1484 646 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 575 2233 2414 2623 575
Size: 1658 181 209 2187
Fragments arranged by size:
2187 1658 209 181
BsrGI T'GTAC_A
Cuts at: 488 488
Size: 4235
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1166 1612 1166
Size: 446 3789
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstXI CCAn_nnnn'nTGG
Cuts at: 1050 1050
Size: 4235
BstYI r'GATC_y
Cuts at: 1 111 235 542 1260 1727 1750 2085 2331
Size: 110 124 307 718 467 23 335 246
Cuts at: 2331 3343 3429 3441 3787 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 718 467 449 346 335 307 246
124 110 86 23 12
BtgI C'CryG_G
Cuts at: 107 371 667 959 107
Size: 264 296 292 3383
Fragments arranged by size:
3383 296 292 264
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 266 425 1478 266
Size: 159 1053 3023
Fragments arranged by size:
3023 1053 159
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 125 158 410 675 707 758 1820 1994 2385
Size: 33 252 265 32 51 1062 174 391
Cuts at: 2385 2412 3757 125
Size: 27 1345 603
Fragments arranged by size:
1345 1062 603 391 265 252 174 51
33 32 27
EagI C'GGCC_G
Cuts at: 410 1820 410
Size: 1410 2825
EarI CTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 2753 2899 4045 2443
Size: 310 146 1146 2633
Fragments arranged by size:
2633 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 682 961 1122 2060 2492 3239 3507 420
Size: 262 279 161 938 432 747 268 1148
Fragments arranged by size:
1148 938 747 432 279 268 262 161
EcoNI CCTnn'n_nnAGG
Cuts at: 245 835 245
Size: 590 3645
EcoO109I rG'GnC_Cy
Cuts at: 290 970 1047 1374 290
Size: 680 77 327 3151
Fragments arranged by size:
3151 680 327 77
EcoRI G'AATT_C
Cuts at: 1239 1239
Size: 4235
EcoRV GAT'ATC
Cuts at: 483 483
Size: 4235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 4235
FspI TGC'GCA
Cuts at: 1207 2016 1207
Size: 809 3426
HaeII r_GCGC'y
Cuts at: 150 540 1669 1917 2939 3918 150
Size: 390 1129 248 1022 979 467
Fragments arranged by size:
1129 1022 979 467 390 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 844 876 2077 2107 2109 2139 655
Size: 32 157 32 1201 30 2 30 2751
Fragments arranged by size:
2751 1201 157 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 146 1665 1913 146
Size: 1519 248 2468
Fragments arranged by size:
2468 1519 248
KpnI G_GTAC'C
Cuts at: 1062 1197 1255 1062
Size: 135 58 4042
Fragments arranged by size:
4042 135 58
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 764 2906 3090 659
Size: 105 2142 184 1804
Fragments arranged by size:
2142 1804 184 105
MscI TGG'CCA
Cuts at: 1996 1996
Size: 4235
MslI CAynn'nnrTG
Cuts at: 370 1021 1025 2224 2351 370
Size: 651 4 1199 127 2254
Fragments arranged by size:
2254 1199 651 127 4
MspA1I CmG'CkG
Cuts at: 152 177 296 386 1142 1346 1660 2020 3033
Size: 25 119 90 756 204 314 360 1013
Cuts at: 3033 3278 152
Size: 245 1109
Fragments arranged by size:
1109 1013 756 360 314 245 204 119
90 25
NaeI GCC'GGC
Cuts at: 577 2416 577
Size: 1839 2396
NarI GG'CG_CC
Cuts at: 147 1666 1914 147
Size: 1519 248 2468
Fragments arranged by size:
2468 1519 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 575 2414 575
Size: 1839 2396
NspI r_CATG'y
Cuts at: 1288 1788 2695 1288
Size: 500 907 2828
Fragments arranged by size:
2828 907 500
PasI CC'CwG_GG
Cuts at: 117 117
Size: 4235
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PmlI CAC'GTG
Cuts at: 1023 1023
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PpuMI rG'GwC_Cy
Cuts at: 290 1047 290
Size: 757 3478
PspOMI G'GGCC_C
Cuts at: 120 969 120
Size: 849 3386
PstI C_TGCA'G
Cuts at: 788 1067 1181 1282 1967 788
Size: 279 114 101 685 3056
Fragments arranged by size:
3056 685 279 114 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 177 1142 1660 2020 177
Size: 965 518 360 2392
Fragments arranged by size:
2392 965 518 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SanDI GG'GwC_CC
Cuts at: 290 290
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 788 1067 1282 788
Size: 279 215 3741
Fragments arranged by size:
3741 279 215
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SfcI C'TryA_G
Cuts at: 33 784 1063 1177 1278 1963 2956 3147 33
Size: 751 279 114 101 685 993 191 1121
Fragments arranged by size:
1121 993 751 685 279 191 114 101
SfoI GGC'GCC
Cuts at: 148 1667 1915 148
Size: 1519 248 2468
Fragments arranged by size:
2468 1519 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 796 1390 2305 2797 3059 796
Size: 594 915 492 262 1972
Fragments arranged by size:
1972 915 594 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
StuI AGG'CCT
Cuts at: 946 1165 946
Size: 219 4016
StyI C'CwwG_G
Cuts at: 107 241 836 1043 1369 1723 107
Size: 134 595 207 326 354 2619
Fragments arranged by size:
2619 595 354 326 207 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 704 730 1818 1951 2410 2619 704
Size: 26 1088 133 459 209 2320
Fragments arranged by size:
2320 1088 459 209 133 26
TatI w'GTAC_w
Cuts at: 488 3750 488
Size: 3262 973
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 3752 3784 264
Size: 32 3456 32 715
Fragments arranged by size:
3456 715 32 32
Tth111I GACn'n_nGTC
Cuts at: 460 858 2032 460
Size: 398 1174 2663
Fragments arranged by size:
2663 1174 398
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 140 717 1730 114
Size: 26 577 1013 2619
Fragments arranged by size:
2619 1013 577 26
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI BaeI
BbsI BbvCI BcgI BfrBI BmtI BseRI BsgI BsiWI
BsmI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI