Restriction map for
pF1KB9897
(Circular) (Six-base) MAPSORT of: pF1KB9897.seq Check: 6092 from: 1 to: 4643
pF1KB9897 4643 bp
With 182 enzymes: SgfI *
March 30, 2011 17:32 ..
AccI GT'mk_AC
Cuts at: 877 1681 877
Size: 804 3839
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 4483 4483
Size: 4643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 580 740 2468 2900 3647 3915 368
Size: 212 160 1728 432 747 268 1096
Fragments arranged by size:
1728 1096 747 432 268 212 160
AflII C'TTAA_G
Cuts at: 1228 1228
Size: 4643
AflIII A'CryG_T
Cuts at: 661 1633 1995 3099 4076 661
Size: 972 362 1104 977 1228
Fragments arranged by size:
1228 1104 977 972 362
AgeI A'CCGG_T
Cuts at: 3031 3031
Size: 4643
AhdI GACnn_n'nnGTC
Cuts at: 636 636
Size: 4643
AleI CACnn'nnGTG
Cuts at: 1634 1634
Size: 4643
AlwNI CAG_nnn'CTG
Cuts at: 1948 3515 1948
Size: 1567 3076
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 259 378 753 1084 1647 3996 4252 259
Size: 119 375 331 563 2349 256 650
Fragments arranged by size:
2349 650 563 375 331 256 119
AseI AT'TA_AT
Cuts at: 20 1213 20
Size: 1193 3450
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 1663 1663
Size: 4643
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4643
BanI G'GyrC_C
Cuts at: 837 1659 2073 2321 2356 837
Size: 822 414 248 35 3124
Fragments arranged by size:
3124 822 414 248 35
BanII G_rGCy'C
Cuts at: 1354 1657 2687 1354
Size: 303 1030 3310
Fragments arranged by size:
3310 1030 303
BbeI G_GCGC'C
Cuts at: 2077 2325 2077
Size: 248 4395
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3302 4643 2541
Size: 761 1341 2541
Fragments arranged by size:
2541 1341 761
BclI T'GATC_A
Cuts at: 930 2163 930
Size: 1233 3410
BglII A'GATC_T
Cuts at: 829 1224 2158 4195 829
Size: 395 934 2037 1277
Fragments arranged by size:
2037 1277 934 395
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4643
Bme1580I G_kGCm'C
Cuts at: 2268 2361 3417 2268
Size: 93 1056 3494
Fragments arranged by size:
3494 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1855 1936 2271 4415 1855
Size: 81 335 2144 2083
Fragments arranged by size:
2144 2083 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4643
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1819 2698 3190 3488 1027
Size: 792 879 492 298 2182
Fragments arranged by size:
2182 879 792 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 805 982 4347 805
Size: 177 3365 1101
Fragments arranged by size:
3365 1101 177
BsaAI yAC'GTr
Cuts at: 662 1996 662
Size: 1334 3309
BsaBI GATnn'nnATC
Cuts at: 6 980 1022 2157 4194 6
Size: 974 42 1135 2037 455
Fragments arranged by size:
2037 1135 974 455 42
BsaHI Gr'CG_yC
Cuts at: 2074 2322 3024 4520 2074
Size: 248 702 1496 2197
Fragments arranged by size:
2197 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1832 2353 3031 3305 3452 3863 1832
Size: 521 678 274 147 411 2612
Fragments arranged by size:
2612 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2068 2826 3403 3899 2068
Size: 758 577 496 2812
Fragments arranged by size:
2812 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2185 2231 3439 104
Size: 2081 46 1208 1308
Fragments arranged by size:
2081 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1657 2435 2625 3417 1657
Size: 778 190 792 2883
Fragments arranged by size:
2883 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1354 1657 2268 2361 2435 2625 2687 3417 1354
Size: 303 611 93 74 190 62 730 2580
Fragments arranged by size:
2580 730 611 303 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3863 3863
Size: 4643
BspHI T'CATG_A
Cuts at: 3819 3819
Size: 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 118 1693 1907 2209 2590 118
Size: 1575 214 302 381 2171
Fragments arranged by size:
2171 1575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2935 2989 4473 4638 50
Size: 2885 54 1484 165 55
Fragments arranged by size:
2885 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 2555
Size: 4643
BsrFI r'CCGG_y
Cuts at: 2641 2822 3031 2641
Size: 181 209 4253
Fragments arranged by size:
4253 209 181
BssSI C'ACGA_G
Cuts at: 2914 3272 2914
Size: 358 4285
BstAPI GCAn_nnn'nTGC
Cuts at: 301 2020 301
Size: 1719 2924
BstBI TT'CG_AA
Cuts at: 3004 3004
Size: 4643
BstYI r'GATC_y
Cuts at: 1 829 1224 1668 2135 2158 2493 2739 3751
Size: 828 395 444 467 23 335 246 1012
Cuts at: 3751 3837 3849 4195 1
Size: 86 12 346 449
Fragments arranged by size:
1012 828 467 449 444 395 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 878 878
Size: 4643
Bsu36I CC'TnA_GG
Cuts at: 1553 1553
Size: 4643
BtgI C'CryG_G
Cuts at: 107 398 107
Size: 291 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2593 2774 2937 2593
Size: 181 163 4299
Fragments arranged by size:
4299 181 163
BtsI GCAGTG_nn'
Cuts at: 1020 1442 1886 1020
Size: 422 444 3777
Fragments arranged by size:
3777 444 422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1169 1204 1169
Size: 35 4608
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 2349 3207 2349
Size: 858 3785
EaeI y'GGCC_r
Cuts at: 575 2228 2402 2793 2820 4165 575
Size: 1653 174 391 27 1345 1053
Fragments arranged by size:
1653 1345 1053 391 174 27
EagI C'GGCC_G
Cuts at: 2228 2228
Size: 4643
EarI CTCTTCn'nnn_
Cuts at: 249 1122 1529 2666 2876 249
Size: 873 407 1137 210 2016
Fragments arranged by size:
2016 1137 873 407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 913 2851 3161 3307 4453 913
Size: 1938 310 146 1146 1103
Fragments arranged by size:
1938 1146 1103 310 146
EcoICRI GAG'CTC
Cuts at: 1655 1655
Size: 4643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 580 740 2468 2900 3647 3915 368
Size: 212 160 1728 432 747 268 1096
Fragments arranged by size:
1728 1096 747 432 268 212 160
EcoNI CCTnn'n_nnAGG
Cuts at: 203 203
Size: 4643
EcoO109I rG'GnC_Cy
Cuts at: 208 1100 1586 1782 208
Size: 892 486 196 3069
Fragments arranged by size:
3069 892 486 196
EcoRI G'AATT_C
Cuts at: 378 1084 1647 378
Size: 706 563 3374
Fragments arranged by size:
3374 706 563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 698 2662 698
Size: 1964 2679
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 2077 2325 3347 4326 2077
Size: 248 1022 979 2394
Fragments arranged by size:
2394 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 622 654 2485 2515 2517 2547 622
Size: 32 1831 30 2 30 2718
Fragments arranged by size:
2718 1831 32 30 30 2
HincII GTy'rAC
Cuts at: 1682 1682
Size: 4643
KasI G'GCGC_C
Cuts at: 2073 2321 2073
Size: 248 4395
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MfeI C'AATT_G
Cuts at: 571 571
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3314 3498 3314
Size: 184 4459
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 953 1078 1436 1634 2632 2759 953
Size: 125 358 198 998 127 2837
Fragments arranged by size:
2837 998 358 198 127 125
MspA1I CmG'CkG
Cuts at: 1754 2068 2428 3441 3686 1754
Size: 314 360 1013 245 2711
Fragments arranged by size:
2711 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2824 2824
Size: 4643
NarI GG'CG_CC
Cuts at: 2074 2322 2074
Size: 248 4395
NcoI C'CATG_G
Cuts at: 107 398 107
Size: 291 4352
NgoMIV G'CCGG_C
Cuts at: 2822 2822
Size: 4643
NspI r_CATG'y
Cuts at: 1637 1696 2196 3103 1637
Size: 59 500 907 3177
Fragments arranged by size:
3177 907 500 59
PciI A'CATG_T
Cuts at: 1633 3099 1633
Size: 1466 3177
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4611
PpuMI rG'GwC_Cy
Cuts at: 208 1100 1586 208
Size: 892 486 3265
Fragments arranged by size:
3265 892 486
PsiI TTA'TAA
Cuts at: 244 506 244
Size: 262 4381
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 929 961 929
Size: 32 4611
PstI C_TGCA'G
Cuts at: 1690 2375 1690
Size: 685 3958
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4643
PvuII CAG'CTG
Cuts at: 2068 2428 2068
Size: 360 4283
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4643
SacI G_AGCT'C
Cuts at: 1657 1657
Size: 4643
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 2666 2876 2666
Size: 210 4433
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 179 4160 179
Size: 3981 662
SfcI C'TryA_G
Cuts at: 33 1528 1686 2371 3364 3555 33
Size: 1495 158 685 993 191 1121
Fragments arranged by size:
1495 1121 993 685 191 158
SfoI GGC'GCC
Cuts at: 2075 2323 2075
Size: 248 4395
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SmaI CCC'GGG
Cuts at: 1665 1665
Size: 4643
SmlI C'TyrA_G
Cuts at: 1006 1228 1798 2713 3205 3467 1006
Size: 222 570 915 492 262 2182
Fragments arranged by size:
2182 915 570 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4643
SphI G_CATG'C
Cuts at: 1696 2196 1696
Size: 500 4143
SspI AAT'ATT
Cuts at: 942 942
Size: 4643
StuI AGG'CCT
Cuts at: 416 416
Size: 4643
StyI C'CwwG_G
Cuts at: 107 398 1103 1777 2131 107
Size: 291 705 674 354 2619
Fragments arranged by size:
2619 705 674 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 855 2226 2359 2818 3027 855
Size: 1371 133 459 209 2471
Fragments arranged by size:
2471 1371 459 209 133
TatI w'GTAC_w
Cuts at: 177 4158 177
Size: 3981 662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1373 1405 4160 4192 1373
Size: 32 2755 32 1824
Fragments arranged by size:
2755 1824 32 32
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4643
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2138
Size: 4643
XmaI C'CCGG_G
Cuts at: 1663 1663
Size: 4643
XmnI GAAnn'nnTTC
Cuts at: 497 1427 497
Size: 930 3713
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI ClaI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI