Restriction map for
pF1KB9887
(Circular) (Six-base) MAPSORT of: pF1KB9887.seq Check: 1951 from: 1 to: 5372
pF1KB9887 5372 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 141 141
Size: 5372
AccI GT'mk_AC
Cuts at: 2410 2410
Size: 5372
Acc65I G'GTAC_C
Cuts at: 2388 2388
Size: 5372
AclI AA'CG_TT
Cuts at: 5212 5212
Size: 5372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 3197 3629 4376 4644 400
Size: 2797 432 747 268 1128
Fragments arranged by size:
2797 1128 747 432 268
AfeI AGC'GCT
Cuts at: 387 400 387
Size: 13 5359
AflII C'TTAA_G
Cuts at: 879 879
Size: 5372
AflIII A'CryG_T
Cuts at: 2724 3828 4805 2724
Size: 1104 977 3291
Fragments arranged by size:
3291 1104 977
AgeI A'CCGG_T
Cuts at: 3760 3760
Size: 5372
AhdI GACnn_n'nnGTC
Cuts at: 1182 1182
Size: 5372
AlwNI CAG_nnn'CTG
Cuts at: 1932 1962 2677 4244 1932
Size: 30 715 1567 3060
Fragments arranged by size:
3060 1567 715 30
ApaI G_GGCC'C
Cuts at: 658 658
Size: 5372
ApaLI G'TGCA_C
Cuts at: 4142 4142
Size: 5372
ApoI r'AATT_y
Cuts at: 1231 2376 4725 4981 1231
Size: 1145 2349 256 1622
Fragments arranged by size:
2349 1622 1145 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 537 649 673 1108 1653 2392 537
Size: 112 24 435 545 739 3517
Fragments arranged by size:
3517 739 545 435 112 24
BamHI G'GATC_C
Cuts at: 2397 2397
Size: 5372
BanI G'GyrC_C
Cuts at: 1467 1683 2271 2388 2802 3050 3085 1467
Size: 216 588 117 414 248 35 3754
Fragments arranged by size:
3754 588 414 248 216 117 35
BanII G_rGCy'C
Cuts at: 472 560 658 665 1195 1493 1660 2386 3416
Size: 88 98 7 530 298 167 726 1030
Cuts at: 3416 472
Size: 2428
Fragments arranged by size:
2428 1030 726 530 298 167 98 88
7
BbeI G_GCGC'C
Cuts at: 2275 2806 3054 2275
Size: 531 248 4593
Fragments arranged by size:
4593 531 248
BbsI GAAGACnn'nnnn_
Cuts at: 1473 1473
Size: 5372
BbvCI CC'TCA_GC
Cuts at: 1031 1226 1738 1031
Size: 195 512 4665
Fragments arranged by size:
4665 512 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1449 1483 1449
Size: 34 5338
BciVI GTATCCnnnnn_n'
Cuts at: 3270 4031 5372 3270
Size: 761 1341 3270
Fragments arranged by size:
3270 1341 761
BclI T'GATC_A
Cuts at: 851 2892 851
Size: 2041 3331
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5372
BglII A'GATC_T
Cuts at: 2887 4924 2887
Size: 2037 3335
BlpI GC'TnA_GC
Cuts at: 2484 2484
Size: 5372
Bme1580I G_kGCm'C
Cuts at: 580 658 788 825 1366 1472 1710 2997 3090
Size: 78 130 37 541 106 238 1287 93
Cuts at: 3090 4146 580
Size: 1056 1806
Fragments arranged by size:
1806 1287 1056 541 238 130 106 93
78 37
BmgBI CAC'GTC
Cuts at: 1746 1746
Size: 5372
BmrI ACTGGGnnnn_n'
Cuts at: 1055 1773 2584 2665 3000 5144 1055
Size: 718 811 81 335 2144 1283
Fragments arranged by size:
2144 1283 811 718 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 491 3739 491
Size: 3248 2124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 1644 1663 2148 992
Size: 652 19 485 4216
Fragments arranged by size:
4216 652 485 19
Bpu10I CC'TnA_GC
Cuts at: 485 659 710 1031 1226 1738 2248 485
Size: 174 51 321 195 512 510 3609
Fragments arranged by size:
3609 512 510 321 195 174 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 2548 3427 3919 4217 1130
Size: 1418 879 492 298 2285
Fragments arranged by size:
2285 1418 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 548 5076 522
Size: 26 4528 818
Fragments arranged by size:
4528 818 26
BsaAI yAC'GTr
Cuts at: 291 1996 2725 291
Size: 1705 729 2938
Fragments arranged by size:
2938 1705 729
BsaBI GATnn'nnATC
Cuts at: 6 2886 4923 6
Size: 2880 2037 455
Fragments arranged by size:
2880 2037 455
BsaHI Gr'CG_yC
Cuts at: 1733 1946 2056 2272 2803 3051 3753 5249 1733
Size: 213 110 216 531 248 702 1496 1856
Fragments arranged by size:
1856 1496 702 531 248 216 213 110
BsaWI w'CCGG_w
Cuts at: 641 1728 1941 2561 3082 3760 4034 4181 4592
Size: 1087 213 620 521 678 274 147 411
Cuts at: 4592 641
Size: 1421
Fragments arranged by size:
1421 1087 678 620 521 411 274 213
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1642 1672 2146 2176 1642
Size: 30 474 30 4838
Fragments arranged by size:
4838 474 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 1015 1126 1225 394
Size: 621 111 99 4541
Fragments arranged by size:
4541 621 111 99
BseYI C'CCAG_C
Cuts at: 591 1471 2797 3555 4132 4628 591
Size: 880 1326 758 577 496 1335
Fragments arranged by size:
1335 1326 880 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 1307 1976 115
Size: 1192 669 3511
Fragments arranged by size:
3511 1192 669
BsiEI CG_ry'CG
Cuts at: 104 606 1074 1137 1439 2914 2960 4168 104
Size: 502 468 63 302 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 502 468 302 63 46
BsiHKAI G_wGCw'C
Cuts at: 341 491 1195 1493 2267 2386 3164 3354 4146
Size: 150 704 298 774 119 778 190 792
Cuts at: 4146 341
Size: 1567
Fragments arranged by size:
1567 792 778 774 704 298 190 150
119
BsmI GAATG_Cn'
Cuts at: 1638 1638
Size: 5372
BsmBI CGTCTCn'nnnn_
Cuts at: 800 2090 800
Size: 1290 4082
Bsp1286I G_dGCh'C
Cuts at: 341 472 491 560 580 658 665 788 825
Size: 131 19 69 20 78 7 123 37
Cuts at: 825 1195 1366 1472 1493 1660 1710 2267 2386
Size: 370 171 106 21 167 50 557 119
Cuts at: 2386 2997 3090 3164 3354 3416 4146 341
Size: 611 93 74 190 62 730 1567
Fragments arranged by size:
1567 730 611 557 370 190 171 167
131 123 119 106 93 78 74 69
62 50 37 21 20 19 7
BspEI T'CCGG_A
Cuts at: 641 1728 1941 4592 641
Size: 1087 213 2651 1421
Fragments arranged by size:
2651 1421 1087 213
BspHI T'CATG_A
Cuts at: 4548 4548
Size: 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 141 1940 2422 2636 2938 3319 141
Size: 1799 482 214 302 381 2194
Fragments arranged by size:
2194 1799 482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3664 3718 5202 5367 50
Size: 3614 54 1484 165 55
Fragments arranged by size:
3614 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2265 3284 2265
Size: 1019 4353
BsrFI r'CCGG_y
Cuts at: 1438 1644 3370 3551 3760 1438
Size: 206 1726 181 209 3050
Fragments arranged by size:
3050 1726 209 206 181
BssSI C'ACGA_G
Cuts at: 340 1367 3643 4001 340
Size: 1027 2276 358 1711
Fragments arranged by size:
2276 1711 1027 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1932 2749 1932
Size: 817 4555
BstBI TT'CG_AA
Cuts at: 3733 3733
Size: 5372
BstXI CCAn_nnnn'nTGG
Cuts at: 2310 2310
Size: 5372
BstYI r'GATC_y
Cuts at: 1 2397 2864 2887 3222 3468 4480 4566 4578
Size: 2396 467 23 335 246 1012 86 12
Cuts at: 4578 4924 1
Size: 346 449
Fragments arranged by size:
2396 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 377 377
Size: 5372
BtgI C'CryG_G
Cuts at: 107 2141 2276 107
Size: 2034 135 3203
Fragments arranged by size:
3203 2034 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3322 3503 3666 3322
Size: 181 163 5028
Fragments arranged by size:
5028 181 163
BtsI GCAGTG_nn'
Cuts at: 143 1319 1708 2615 143
Size: 1176 389 907 2900
Fragments arranged by size:
2900 1176 907 389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1308 1343 1308
Size: 35 5337
DraI TTT'AAA
Cuts at: 2371 2371
Size: 5372
DraIII CAC_nnn'GTG
Cuts at: 138 138
Size: 5372
DrdI GACnn_nn'nnGTC
Cuts at: 3078 3936 3078
Size: 858 4514
EaeI y'GGCC_r
Cuts at: 161 603 978 1071 1436 2048 2279 2324 2957
Size: 442 375 93 365 612 231 45 633
Cuts at: 2957 3131 3522 3549 4894 161
Size: 174 391 27 1345 639
Fragments arranged by size:
1345 639 633 612 442 391 375 365
231 174 93 45 27
EagI C'GGCC_G
Cuts at: 603 1071 1436 2957 603
Size: 468 365 1521 3018
Fragments arranged by size:
3018 1521 468 365
EarI CTCTTCn'nnn_
Cuts at: 419 684 1018 1048 1590 3395 3605 419
Size: 265 334 30 542 1805 210 2186
Fragments arranged by size:
2186 1805 542 334 265 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 201 256 1440 3580 3890 4036 5182 201
Size: 55 1184 2140 310 146 1146 391
Fragments arranged by size:
2140 1184 1146 391 310 146 55
EcoICRI GAG'CTC
Cuts at: 1193 1491 2384 1193
Size: 298 893 4181
Fragments arranged by size:
4181 893 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 992 1644 1663 2148 3197 3629 4376 4644
Size: 592 652 19 485 1049 432 747 268
Cuts at: 4644 400
Size: 1128
Fragments arranged by size:
1128 1049 747 652 592 485 432 268
19
EcoNI CCTnn'n_nnAGG
Cuts at: 1201 1504 1201
Size: 303 5069
EcoO109I rG'GnC_Cy
Cuts at: 372 654 655 872 1077 1497 1755 2511 372
Size: 282 1 217 205 420 258 756 3233
Fragments arranged by size:
3233 756 420 282 258 217 205 1
EcoRI G'AATT_C
Cuts at: 1231 2376 1231
Size: 1145 4227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1141 3391 1141
Size: 2250 3122
FspI TGC'GCA
Cuts at: 3153 3153
Size: 5372
HaeII r_GCGC'y
Cuts at: 273 389 402 1445 2275 2806 3054 4076 5055
Size: 116 13 1043 830 531 248 1022 979
Cuts at: 5055 273
Size: 590
Fragments arranged by size:
1043 1022 979 830 590 531 248 116
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 823 855 1047 1079 1168 1200 1534 1566 1785
Size: 32 192 32 89 32 334 32 219
Cuts at: 1785 1817 3214 3244 3246 3276 823
Size: 32 1397 30 2 30 2919
Fragments arranged by size:
2919 1397 334 219 192 89 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2411 2411
Size: 5372
KasI G'GCGC_C
Cuts at: 2271 2802 3050 2271
Size: 531 248 4593
Fragments arranged by size:
4593 531 248
KpnI G_GTAC'C
Cuts at: 2392 2392
Size: 5372
MluI A'CGCG_T
Cuts at: 4805 4805
Size: 5372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 646 1154 4043 4227 646
Size: 508 2889 184 1791
Fragments arranged by size:
2889 1791 508 184
MscI TGG'CCA
Cuts at: 163 2050 2326 3133 163
Size: 1887 276 807 2402
Fragments arranged by size:
2402 1887 807 276
MslI CAynn'nnrTG
Cuts at: 3361 3488 3361
Size: 127 5245
MspA1I CmG'CkG
Cuts at: 595 667 977 1806 1959 2143 2180 2278 2319
Size: 72 310 829 153 184 37 98 41
Cuts at: 2319 2483 2797 3157 4170 4415 595
Size: 164 314 360 1013 245 1552
Fragments arranged by size:
1552 1013 829 360 314 310 245 184
164 153 98 72 41 37
NaeI GCC'GGC
Cuts at: 1440 3553 1440
Size: 2113 3259
NarI GG'CG_CC
Cuts at: 2272 2803 3051 2272
Size: 531 248 4593
Fragments arranged by size:
4593 531 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5372
NgoMIV G'CCGG_C
Cuts at: 1438 3551 1438
Size: 2113 3259
NruI TCG'CGA
Cuts at: 554 554
Size: 5372
NspI r_CATG'y
Cuts at: 2425 2925 3832 2425
Size: 500 907 3965
Fragments arranged by size:
3965 907 500
PasI CC'CwG_GG
Cuts at: 753 825 753
Size: 72 5300
PciI A'CATG_T
Cuts at: 3828 3828
Size: 5372
PflMI CCAn_nnn'nTGG
Cuts at: 291 1087 291
Size: 796 4576
PfoI T'CCnGG_A
Cuts at: 1406 1598 1877 1406
Size: 192 279 4901
Fragments arranged by size:
4901 279 192
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PmlI CAC'GTG
Cuts at: 291 1996 291
Size: 1705 3667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 5340
PpuMI rG'GwC_Cy
Cuts at: 372 1077 1497 1755 372
Size: 705 420 258 3989
Fragments arranged by size:
3989 705 420 258
PspOMI G'GGCC_C
Cuts at: 654 654
Size: 5372
PstI C_TGCA'G
Cuts at: 152 1433 2094 2419 3104 152
Size: 1281 661 325 685 2420
Fragments arranged by size:
2420 1281 685 661 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5372
PvuII CAG'CTG
Cuts at: 595 1806 2797 3157 595
Size: 1211 991 360 2810
Fragments arranged by size:
2810 1211 991 360
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 1195 1493 2386 1195
Size: 298 893 4181
Fragments arranged by size:
4181 893 298
SacII CC_GC'GG
Cuts at: 2144 2279 2144
Size: 135 5237
SalI G'TCGA_C
Cuts at: 2409 2409
Size: 5372
SanDI GG'GwC_CC
Cuts at: 1077 1077
Size: 5372
SapI GCTCTTCn'nnn_
Cuts at: 419 3395 3605 419
Size: 2976 210 2186
Fragments arranged by size:
2976 2186 210
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 4889 4889
Size: 5372
SfcI C'TryA_G
Cuts at: 33 148 1429 2090 2415 3100 4093 4284 33
Size: 115 1281 661 325 685 993 191 1121
Fragments arranged by size:
1281 1121 993 685 661 325 191 115
SfoI GGC'GCC
Cuts at: 2273 2804 3052 2273
Size: 531 248 4593
Fragments arranged by size:
4593 531 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 675 2394 675
Size: 1719 3653
SmlI C'TyrA_G
Cuts at: 879 1145 2527 3442 3934 4196 879
Size: 266 1382 915 492 262 2055
Fragments arranged by size:
2055 1382 915 492 266 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 2425 2925 2425
Size: 500 4872
SspI AAT'ATT
Cuts at: 196 196
Size: 5372
StuI AGG'CCT
Cuts at: 433 1029 1510 1665 433
Size: 596 481 155 4140
Fragments arranged by size:
4140 596 481 155
StyI C'CwwG_G
Cuts at: 107 895 995 1358 2327 2506 2860 107
Size: 788 100 363 969 179 354 2619
Fragments arranged by size:
2619 969 788 363 354 179 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 362 2955 3088 3547 3756 362
Size: 2593 133 459 209 1978
Fragments arranged by size:
2593 1978 459 209 133
TatI w'GTAC_w
Cuts at: 1649 4887 1649
Size: 3238 2134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1580 1612 1613 1645 2146 2178 4889 4921 1580
Size: 32 1 32 501 32 2711 32 2031
Fragments arranged by size:
2711 2031 501 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 3169 3169
Size: 5372
XbaI T'CTAG_A
Cuts at: 1065 2403 1065
Size: 1338 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2867 2867
Size: 5372
XmaI C'CCGG_G
Cuts at: 673 2392 673
Size: 1719 3653
XmnI GAAnn'nnTTC
Cuts at: 1778 1778
Size: 5372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI