Restriction map for
pF1KB9881
(Circular) (Six-base) MAPSORT of: pF1KB9881.seq Check: 3004 from: 1 to: 4910
pF1KB9881 4910 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 307 1092 307
Size: 785 4125
AccI GT'mk_AC
Cuts at: 1948 1948
Size: 4910
Acc65I G'GTAC_C
Cuts at: 1775 1926 1775
Size: 151 4759
AclI AA'CG_TT
Cuts at: 4750 4750
Size: 4910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 2735 3167 3914 4182 265
Size: 2470 432 747 268 993
Fragments arranged by size:
2470 993 747 432 268
AflIII A'CryG_T
Cuts at: 677 2262 3366 4343 677
Size: 1585 1104 977 1244
Fragments arranged by size:
1585 1244 1104 977
AgeI A'CCGG_T
Cuts at: 3298 3298
Size: 4910
AlwNI CAG_nnn'CTG
Cuts at: 780 927 2215 3782 780
Size: 147 1288 1567 1908
Fragments arranged by size:
1908 1567 1288 147
ApaI G_GGCC'C
Cuts at: 115 1539 115
Size: 1424 3486
ApaLI G'TGCA_C
Cuts at: 3680 3680
Size: 4910
ApoI r'AATT_y
Cuts at: 1914 4263 4519 1914
Size: 2349 256 2305
Fragments arranged by size:
2349 2305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4910
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
AvaI C'yCGr_G
Cuts at: 337 629 1026 1930 337
Size: 292 397 904 3317
Fragments arranged by size:
3317 904 397 292
BamHI G'GATC_C
Cuts at: 1164 1935 1164
Size: 771 4139
BanI G'GyrC_C
Cuts at: 491 564 585 1066 1579 1722 1775 1926 2340
Size: 73 21 481 513 143 53 151 414
Cuts at: 2340 2588 2623 491
Size: 248 35 2778
Fragments arranged by size:
2778 513 481 414 248 151 143 73
53 35 21
BanII G_rGCy'C
Cuts at: 115 1507 1539 1924 2954 115
Size: 1392 32 385 1030 2071
Fragments arranged by size:
2071 1392 1030 385 32
BbeI G_GCGC'C
Cuts at: 495 568 2344 2592 495
Size: 73 1776 248 2813
Fragments arranged by size:
2813 1776 248 73
BbsI GAAGACnn'nnnn_
Cuts at: 1694 1694
Size: 4910
BbvCI CC'TCA_GC
Cuts at: 1526 1526
Size: 4910
BciVI GTATCCnnnnn_n'
Cuts at: 2808 3569 4910 2808
Size: 761 1341 2808
Fragments arranged by size:
2808 1341 761
BclI T'GATC_A
Cuts at: 2430 2430
Size: 4910
BglI GCCn_nnn'nGGC
Cuts at: 265 577 872 1225 1873 265
Size: 312 295 353 648 3302
Fragments arranged by size:
3302 648 353 312 295
BglII A'GATC_T
Cuts at: 2425 4462 2425
Size: 2037 2873
BlpI GC'TnA_GC
Cuts at: 232 2022 232
Size: 1790 3120
Bme1580I G_kGCm'C
Cuts at: 115 590 1539 1584 1603 2535 2628 3684 115
Size: 475 949 45 19 932 93 1056 1341
Fragments arranged by size:
1341 1056 949 932 475 93 45 19
BmgBI CAC'GTC
Cuts at: 678 678
Size: 4910
BmrI ACTGGGnnnn_n'
Cuts at: 1005 1542 1590 2122 2203 2538 4682 1005
Size: 537 48 532 81 335 2144 1233
Fragments arranged by size:
2144 1233 537 532 335 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1521 1524 3277 1521
Size: 3 1753 3154
Fragments arranged by size:
3154 1753 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1102
Size: 4910
Bpu10I CC'TnA_GC
Cuts at: 781 1526 781
Size: 745 4165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1120 1294 2086 2965 3457 3755 391
Size: 729 174 792 879 492 298 1546
Fragments arranged by size:
1546 879 792 729 492 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 688 1192 4614 688
Size: 504 3422 984
Fragments arranged by size:
3422 984 504
BsaAI yAC'GTr
Cuts at: 2263 2263
Size: 4910
BsaBI GATnn'nnATC
Cuts at: 6 2424 4461 6
Size: 2418 2037 455
Fragments arranged by size:
2418 2037 455
BsaHI Gr'CG_yC
Cuts at: 268 492 565 2341 2589 3291 4787 268
Size: 224 73 1776 248 702 1496 391
Fragments arranged by size:
1776 1496 702 391 248 224 73
BsaWI w'CCGG_w
Cuts at: 177 2099 2620 3298 3572 3719 4130 177
Size: 1922 521 678 274 147 411 957
Fragments arranged by size:
1922 957 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 265 295 1768 1798 265
Size: 30 1473 30 3377
Fragments arranged by size:
3377 1473 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 448 649 1345 1348 1351 1354 1366 1369
Size: 264 201 696 3 3 3 12 3
Cuts at: 1369 1396 1399 1411 1414 1417 1420 1459 1468
Size: 27 3 12 3 3 3 39 9
Cuts at: 1468 1471 1497 1501 1513 1720 184
Size: 3 26 4 12 207 3374
Fragments arranged by size:
3374 696 264 207 201 39 27 26
12 12 12 9 4 3 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 438 773 1182 1732 2335 3093 3670 4166 438
Size: 335 409 550 603 758 577 496 1182
Fragments arranged by size:
1182 758 603 577 550 496 409 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1186 1828 1063
Size: 123 642 4145
Fragments arranged by size:
4145 642 123
BsiEI CG_ry'CG
Cuts at: 104 581 760 999 2452 2498 3706 104
Size: 477 179 239 1453 46 1208 1308
Fragments arranged by size:
1453 1308 1208 477 239 179 46
BsiHKAI G_wGCw'C
Cuts at: 238 613 1507 1924 2702 2892 3684 238
Size: 375 894 417 778 190 792 1464
Fragments arranged by size:
1464 894 792 778 417 375 190
Bsp1286I G_dGCh'C
Cuts at: 115 238 590 613 1507 1539 1584 1603 1924
Size: 123 352 23 894 32 45 19 321
Cuts at: 1924 2535 2628 2702 2892 2954 3684 115
Size: 611 93 74 190 62 730 1341
Fragments arranged by size:
1341 894 730 611 352 321 190 123
93 74 62 45 32 23 19
BspEI T'CCGG_A
Cuts at: 177 4130 177
Size: 3953 957
BspHI T'CATG_A
Cuts at: 4086 4086
Size: 4910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 307 1092 1960 2174 2476 2857 191
Size: 116 785 868 214 302 381 2244
Fragments arranged by size:
2244 868 785 381 302 214 116
BsrBI CCG'CTC
Cuts at: 50 3202 3256 4740 4905 50
Size: 3152 54 1484 165 55
Fragments arranged by size:
3152 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2822 2822
Size: 4910
BsrFI r'CCGG_y
Cuts at: 379 387 488 838 871 994 1555 2908 3089
Size: 8 101 350 33 123 561 1353 181
Cuts at: 3089 3298 379
Size: 209 1991
Fragments arranged by size:
1991 1353 561 350 209 181 123 101
33 8
BssHII G'CGCG_C
Cuts at: 255 533 624 255
Size: 278 91 4541
Fragments arranged by size:
4541 278 91
BssSI C'ACGA_G
Cuts at: 3181 3539 3181
Size: 358 4552
BstAPI GCAn_nnn'nTGC
Cuts at: 2287 2287
Size: 4910
BstBI TT'CG_AA
Cuts at: 3271 3271
Size: 4910
BstYI r'GATC_y
Cuts at: 1 368 1164 1935 2402 2425 2760 3006 4018
Size: 367 796 771 467 23 335 246 1012
Cuts at: 4018 4104 4116 4462 1
Size: 86 12 346 449
Fragments arranged by size:
1012 796 771 467 449 367 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1170 1170
Size: 4910
BtgI C'CryG_G
Cuts at: 107 495 581 731 1142 1748 1763 107
Size: 388 86 150 411 606 15 3254
Fragments arranged by size:
3254 606 411 388 150 86 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 336 2860 3041 3204 336
Size: 2524 181 163 2042
Fragments arranged by size:
2524 2042 181 163
BtsI GCAGTG_nn'
Cuts at: 606 1235 2153 606
Size: 629 918 3363
Fragments arranged by size:
3363 918 629
DraI TTT'AAA
Cuts at: 1909 1909
Size: 4910
DraIII CAC_nnn'GTG
Cuts at: 501 1209 501
Size: 708 4202
DrdI GACnn_nn'nnGTC
Cuts at: 761 2616 3474 761
Size: 1855 858 2197
Fragments arranged by size:
2197 1855 858
EaeI y'GGCC_r
Cuts at: 578 653 757 836 848 929 992 996 1145
Size: 75 104 79 12 81 63 4 149
Cuts at: 1145 2495 2669 3060 3087 4432 578
Size: 1350 174 391 27 1345 1056
Fragments arranged by size:
1350 1345 1056 391 174 149 104 81
79 75 63 27 12 4
EagI C'GGCC_G
Cuts at: 578 757 996 2495 578
Size: 179 239 1499 2993
Fragments arranged by size:
2993 1499 239 179
EarI CTCTTCn'nnn_
Cuts at: 1322 1343 1355 1373 1436 1478 1649 1664 2933
Size: 21 12 18 63 42 171 15 1269
Cuts at: 2933 3143 1322
Size: 210 3089
Fragments arranged by size:
3089 1269 210 171 63 42 21 18
15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 3118 3428 3574 4720 475
Size: 2643 310 146 1146 665
Fragments arranged by size:
2643 1146 665 310 146
EcoICRI GAG'CTC
Cuts at: 1505 1922 1505
Size: 417 4493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 1102 2735 3167 3914 4182 265
Size: 837 1633 432 747 268 993
Fragments arranged by size:
1633 993 837 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 187 187
Size: 4910
EcoO109I rG'GnC_Cy
Cuts at: 112 924 981 1197 2049 112
Size: 812 57 216 852 2973
Fragments arranged by size:
2973 852 812 216 57
EcoRI G'AATT_C
Cuts at: 1914 1914
Size: 4910
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 4910
FseI GG_CCGG'CC
Cuts at: 998 998
Size: 4910
FspI TGC'GCA
Cuts at: 187 424 2691 187
Size: 237 2267 2406
Fragments arranged by size:
2406 2267 237
HaeII r_GCGC'y
Cuts at: 210 421 495 568 689 780 2344 2592 3614
Size: 211 74 73 121 91 1564 248 1022
Cuts at: 3614 4593 210
Size: 979 527
Fragments arranged by size:
1564 1022 979 527 248 211 121 91
74 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2752 2782 2784 2814 2752
Size: 30 2 30 4848
Fragments arranged by size:
4848 30 30 2
HincII GTy'rAC
Cuts at: 1949 1949
Size: 4910
KasI G'GCGC_C
Cuts at: 491 564 2340 2588 491
Size: 73 1776 248 2813
Fragments arranged by size:
2813 1776 248 73
KpnI G_GTAC'C
Cuts at: 1779 1930 1779
Size: 151 4759
MluI A'CGCG_T
Cuts at: 4343 4343
Size: 4910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 251 488 3581 3765 251
Size: 237 3093 184 1396
Fragments arranged by size:
3093 1396 237 184
MscI TGG'CCA
Cuts at: 931 2671 931
Size: 1740 3170
MslI CAynn'nnrTG
Cuts at: 958 2899 3026 958
Size: 1941 127 2842
Fragments arranged by size:
2842 1941 127
MspA1I CmG'CkG
Cuts at: 129 515 583 733 831 1047 1080 1182 2021
Size: 386 68 150 98 216 33 102 839
Cuts at: 2021 2335 2695 3708 3953 129
Size: 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 839 386 360 314 245 216
150 102 98 68 33
NaeI GCC'GGC
Cuts at: 381 490 840 873 996 3091 381
Size: 109 350 33 123 2095 2200
Fragments arranged by size:
2200 2095 350 123 109 33
NarI GG'CG_CC
Cuts at: 492 565 2341 2589 492
Size: 73 1776 248 2813
Fragments arranged by size:
2813 1776 248 73
NcoI C'CATG_G
Cuts at: 107 1142 1748 1763 107
Size: 1035 606 15 3254
Fragments arranged by size:
3254 1035 606 15
NgoMIV G'CCGG_C
Cuts at: 379 488 838 871 994 3089 379
Size: 109 350 33 123 2095 2200
Fragments arranged by size:
2200 2095 350 123 109 33
NspI r_CATG'y
Cuts at: 1042 1963 2463 3370 1042
Size: 921 500 907 2582
Fragments arranged by size:
2582 921 907 500
PasI CC'CwG_GG
Cuts at: 1539 1539
Size: 4910
PciI A'CATG_T
Cuts at: 3366 3366
Size: 4910
PflMI CCAn_nnn'nTGG
Cuts at: 165 470 501 704 927 1017 165
Size: 305 31 203 223 90 4058
Fragments arranged by size:
4058 305 223 203 90 31
PfoI T'CCnGG_A
Cuts at: 137 158 1616 137
Size: 21 1458 3431
Fragments arranged by size:
3431 1458 21
PmeI GTTT'AAAC
Cuts at: 1909 1909
Size: 4910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4878
PpuMI rG'GwC_Cy
Cuts at: 981 981
Size: 4910
PspOMI G'GGCC_C
Cuts at: 111 1535 111
Size: 1424 3486
PspXI vC'TCGA_Gb
Cuts at: 337 337
Size: 4910
PstI C_TGCA'G
Cuts at: 514 969 1181 1957 2642 514
Size: 455 212 776 685 2782
Fragments arranged by size:
2782 776 685 455 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4910
PvuII CAG'CTG
Cuts at: 129 1047 1182 2335 2695 129
Size: 918 135 1153 360 2344
Fragments arranged by size:
2344 1153 918 360 135
RsrII CG'GwC_CG
Cuts at: 3105 3105
Size: 4910
SacI G_AGCT'C
Cuts at: 1507 1924 1507
Size: 417 4493
SacII CC_GC'GG
Cuts at: 584 734 584
Size: 150 4760
SalI G'TCGA_C
Cuts at: 1947 1947
Size: 4910
SapI GCTCTTCn'nnn_
Cuts at: 2933 3143 2933
Size: 210 4700
SbfI CC_TGCA'GG
Cuts at: 1957 1957
Size: 4910
ScaI AGT'ACT
Cuts at: 937 4427 937
Size: 3490 1420
SfcI C'TryA_G
Cuts at: 33 510 965 1177 1953 2638 3631 3822 33
Size: 477 455 212 776 685 993 191 1121
Fragments arranged by size:
1121 993 776 685 477 455 212 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 872 872
Size: 4910
SfoI GGC'GCC
Cuts at: 493 566 2342 2590 493
Size: 73 1776 248 2813
Fragments arranged by size:
2813 1776 248 73
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
SgrAI Cr'CCGG_yG
Cuts at: 488 488
Size: 4910
SmaI CCC'GGG
Cuts at: 1932 1932
Size: 4910
SmlI C'TyrA_G
Cuts at: 337 406 1135 1309 2065 2980 3472 3734 337
Size: 69 729 174 756 915 492 262 1513
Fragments arranged by size:
1513 915 756 729 492 262 174 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4910
SphI G_CATG'C
Cuts at: 1963 2463 1963
Size: 500 4410
StuI AGG'CCT
Cuts at: 946 1175 946
Size: 229 4681
StyI C'CwwG_G
Cuts at: 107 902 947 1016 1142 1748 1763 2044 2398
Size: 795 45 69 126 606 15 281 354
Cuts at: 2398 107
Size: 2619
Fragments arranged by size:
2619 795 606 354 281 126 69 45
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 787 934 2493 2626 3085 3294 787
Size: 147 1559 133 459 209 2403
Fragments arranged by size:
2403 1559 459 209 147 133
TatI w'GTAC_w
Cuts at: 935 4425 935
Size: 3490 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 140 172 1059 1091 1582 1614 4427 4459 140
Size: 32 887 32 491 32 2813 32 591
Fragments arranged by size:
2813 887 591 491 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2707 2707
Size: 4910
XbaI T'CTAG_A
Cuts at: 1941 1941
Size: 4910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 471 924 2405 471
Size: 453 1481 2976
Fragments arranged by size:
2976 1481 453
XhoI C'TCGA_G
Cuts at: 337 337
Size: 4910
XmaI C'CCGG_G
Cuts at: 1930 1930
Size: 4910
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PsrI SanDI SexAI SnaBI
SrfI SspI SwaI XmnI ZraI