Restriction map for
pF1KB9870
(Circular) (Six-base) MAPSORT of: pF1KB9870.seq Check: 951 from: 1 to: 4544
pF1KB9870 4544 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 326 326
Size: 4544
AatII G_ACGT'C
Cuts at: 452 1136 1438 452
Size: 684 302 3558
Fragments arranged by size:
3558 684 302
AccI GT'mk_AC
Cuts at: 1582 1582
Size: 4544
Acc65I G'GTAC_C
Cuts at: 1560 1560
Size: 4544
AclI AA'CG_TT
Cuts at: 4384 4384
Size: 4544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 2801 3548 3816 2369
Size: 432 747 268 3097
Fragments arranged by size:
3097 747 432 268
AflIII A'CryG_T
Cuts at: 1896 3000 3977 1896
Size: 1104 977 2463
Fragments arranged by size:
2463 1104 977
AgeI A'CCGG_T
Cuts at: 2932 2932
Size: 4544
AleI CACnn'nnGTG
Cuts at: 1255 1255
Size: 4544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 745
Size: 32 4512
AlwNI CAG_nnn'CTG
Cuts at: 168 1224 1849 3416 168
Size: 1056 625 1567 1296
Fragments arranged by size:
1567 1296 1056 625
ApaI G_GGCC'C
Cuts at: 221 221
Size: 4544
ApaLI G'TGCA_C
Cuts at: 3314 3314
Size: 4544
ApoI r'AATT_y
Cuts at: 1548 3897 4153 1548
Size: 2349 256 1939
Fragments arranged by size:
2349 1939 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4544
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
AvaI C'yCGr_G
Cuts at: 141 1564 141
Size: 1423 3121
BamHI G'GATC_C
Cuts at: 1569 1569
Size: 4544
BanI G'GyrC_C
Cuts at: 130 204 1560 1974 2222 2257 130
Size: 74 1356 414 248 35 2417
Fragments arranged by size:
2417 1356 414 248 74 35
BanII G_rGCy'C
Cuts at: 196 221 737 1286 1558 2588 196
Size: 25 516 549 272 1030 2152
Fragments arranged by size:
2152 1030 549 516 272 25
BbeI G_GCGC'C
Cuts at: 1978 2226 1978
Size: 248 4296
BbsI GAAGACnn'nnnn_
Cuts at: 333 1011 1128 1302 333
Size: 678 117 174 3575
Fragments arranged by size:
3575 678 174 117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 727 761 90
Size: 34 603 34 3873
Fragments arranged by size:
3873 603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3203 4544 2442
Size: 761 1341 2442
Fragments arranged by size:
2442 1341 761
BclI T'GATC_A
Cuts at: 1007 2064 1007
Size: 1057 3487
BglI GCCn_nnn'nGGC
Cuts at: 216 415 216
Size: 199 4345
BglII A'GATC_T
Cuts at: 1424 2059 4096 1424
Size: 635 2037 1872
Fragments arranged by size:
2037 1872 635
BlpI GC'TnA_GC
Cuts at: 1656 1656
Size: 4544
Bme1580I G_kGCm'C
Cuts at: 207 221 2169 2262 3318 207
Size: 14 1948 93 1056 1433
Fragments arranged by size:
1948 1433 1056 93 14
BmgBI CAC'GTC
Cuts at: 741 741
Size: 4544
BmrI ACTGGGnnnn_n'
Cuts at: 223 534 1756 1837 2172 4316 223
Size: 311 1222 81 335 2144 451
Fragments arranged by size:
2144 1222 451 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1305 2911 1305
Size: 1606 2938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 747 913 519
Size: 228 166 4150
Fragments arranged by size:
4150 228 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1720 2599 3091 3389 976
Size: 744 879 492 298 2131
Fragments arranged by size:
2131 879 744 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 973 1114 4248 973
Size: 141 3134 1269
Fragments arranged by size:
3134 1269 141
BsaAI yAC'GTr
Cuts at: 1897 1897
Size: 4544
BsaBI GATnn'nnATC
Cuts at: 6 2058 4095 6
Size: 2052 2037 455
Fragments arranged by size:
2052 2037 455
BsaHI Gr'CG_yC
Cuts at: 449 709 1133 1435 1975 2223 2925 4421 449
Size: 260 424 302 540 248 702 1496 572
Fragments arranged by size:
1496 702 572 540 424 302 260 248
BsaWI w'CCGG_w
Cuts at: 630 1733 2254 2932 3206 3353 3764 630
Size: 1103 521 678 274 147 411 1410
Fragments arranged by size:
1410 1103 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 1228 1258 745
Size: 30 453 30 4031
Fragments arranged by size:
4031 453 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 733 1152 1408 1411 733
Size: 419 256 3 3866
Fragments arranged by size:
3866 419 256 3
BseYI C'CCAG_C
Cuts at: 273 1151 1217 1224 1969 2727 3304 3800 273
Size: 878 66 7 745 758 577 496 1017
Fragments arranged by size:
1017 878 758 745 577 496 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1251 889
Size: 362 4182
BsiEI CG_ry'CG
Cuts at: 104 1100 1413 2086 2132 3340 104
Size: 996 313 673 46 1208 1308
Fragments arranged by size:
1308 1208 996 673 313 46
BsiHKAI G_wGCw'C
Cuts at: 478 911 1286 1558 2336 2526 3318 478
Size: 433 375 272 778 190 792 1704
Fragments arranged by size:
1704 792 778 433 375 272 190
BsmI GAATG_Cn'
Cuts at: 750 750
Size: 4544
BsmBI CGTCTCn'nnnn_
Cuts at: 1255 1255
Size: 4544
Bsp1286I G_dGCh'C
Cuts at: 196 207 221 478 737 911 1286 1558 2169
Size: 11 14 257 259 174 375 272 611
Cuts at: 2169 2262 2336 2526 2588 3318 196
Size: 93 74 190 62 730 1422
Fragments arranged by size:
1422 730 611 375 272 259 257 190
174 93 74 62 14 11
BspEI T'CCGG_A
Cuts at: 3764 3764
Size: 4544
BspHI T'CATG_A
Cuts at: 827 3720 827
Size: 2893 1651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 692 1594 1808 2110 2491 326
Size: 366 902 214 302 381 2379
Fragments arranged by size:
2379 902 381 366 302 214
BsrBI CCG'CTC
Cuts at: 50 2836 2890 4374 4539 50
Size: 2786 54 1484 165 55
Fragments arranged by size:
2786 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1284 2456 1284
Size: 1172 3372
BsrFI r'CCGG_y
Cuts at: 418 1099 1254 2542 2723 2932 418
Size: 681 155 1288 181 209 2030
Fragments arranged by size:
2030 1288 681 209 181 155
BssHII G'CGCG_C
Cuts at: 321 941 960 321
Size: 620 19 3905
Fragments arranged by size:
3905 620 19
BssSI C'ACGA_G
Cuts at: 2815 3173 2815
Size: 358 4186
BstAPI GCAn_nnn'nTGC
Cuts at: 1921 1921
Size: 4544
BstBI TT'CG_AA
Cuts at: 2905 2905
Size: 4544
BstEII G'GTnAC_C
Cuts at: 231 1447 231
Size: 1216 3328
BstYI r'GATC_y
Cuts at: 1 872 1424 1569 2036 2059 2394 2640 3652
Size: 871 552 145 467 23 335 246 1012
Cuts at: 3652 3738 3750 4096 1
Size: 86 12 346 449
Fragments arranged by size:
1012 871 552 467 449 346 335 246
145 86 23 12
BtgI C'CryG_G
Cuts at: 185 185
Size: 4544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 843 2494 2675 2838 843
Size: 1651 181 163 2549
Fragments arranged by size:
2549 1651 181 163
BtsI GCAGTG_nn'
Cuts at: 479 1080 1787 479
Size: 601 707 3236
Fragments arranged by size:
3236 707 601
DraI TTT'AAA
Cuts at: 1543 1543
Size: 4544
DrdI GACnn_nn'nnGTC
Cuts at: 1138 2250 3108 1138
Size: 1112 858 2574
Fragments arranged by size:
2574 1112 858
EaeI y'GGCC_r
Cuts at: 356 416 698 1097 2129 2303 2694 2721 4066
Size: 60 282 399 1032 174 391 27 1345
Cuts at: 4066 356
Size: 834
Fragments arranged by size:
1345 1032 834 399 391 282 174 60
27
EagI C'GGCC_G
Cuts at: 1097 2129 1097
Size: 1032 3512
EarI CTCTTCn'nnn_
Cuts at: 795 2567 2777 795
Size: 1772 210 2562
Fragments arranged by size:
2562 1772 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2752 3062 3208 4354 2752
Size: 310 146 1146 2942
Fragments arranged by size:
2942 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1284 1556 1284
Size: 272 4272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 747 913 2369 2801 3548 3816 519
Size: 228 166 1456 432 747 268 1247
Fragments arranged by size:
1456 1247 747 432 268 228 166
EcoO109I rG'GnC_Cy
Cuts at: 217 218 539 1090 1402 1683 217
Size: 1 321 551 312 281 3078
Fragments arranged by size:
3078 551 321 312 281 1
EcoRI G'AATT_C
Cuts at: 1548 1548
Size: 4544
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 4544
FspI TGC'GCA
Cuts at: 294 2325 294
Size: 2031 2513
HaeII r_GCGC'y
Cuts at: 382 1978 2226 3248 4227 382
Size: 1596 248 1022 979 699
Fragments arranged by size:
1596 1022 979 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2386 2416 2418 2448 2386
Size: 30 2 30 4482
Fragments arranged by size:
4482 30 30 2
HincII GTy'rAC
Cuts at: 1583 1583
Size: 4544
KasI G'GCGC_C
Cuts at: 1974 2222 1974
Size: 248 4296
KpnI G_GTAC'C
Cuts at: 1564 1564
Size: 4544
MluI A'CGCG_T
Cuts at: 3977 3977
Size: 4544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3215 3399 3215
Size: 184 4360
MscI TGG'CCA
Cuts at: 700 2305 700
Size: 1605 2939
MslI CAynn'nnrTG
Cuts at: 511 832 1255 1342 2533 2660 511
Size: 321 423 87 1191 127 2395
Fragments arranged by size:
2395 1191 423 321 127 87
MspA1I CmG'CkG
Cuts at: 168 1221 1330 1655 1969 2329 3342 3587 168
Size: 1053 109 325 314 360 1013 245 1125
Fragments arranged by size:
1125 1053 1013 360 325 314 245 109
NaeI GCC'GGC
Cuts at: 1101 2725 1101
Size: 1624 2920
NarI GG'CG_CC
Cuts at: 1975 2223 1975
Size: 248 4296
NgoMIV G'CCGG_C
Cuts at: 1099 2723 1099
Size: 1624 2920
NspI r_CATG'y
Cuts at: 1419 1597 2097 3004 1419
Size: 178 500 907 2959
Fragments arranged by size:
2959 907 500 178
PasI CC'CwG_GG
Cuts at: 713 713
Size: 4544
PciI A'CATG_T
Cuts at: 3000 3000
Size: 4544
PflMI CCAn_nnn'nTGG
Cuts at: 168 1224 168
Size: 1056 3488
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 745 777 3683 3715 745
Size: 32 2906 32 1574
Fragments arranged by size:
2906 1574 32 32
PpuMI rG'GwC_Cy
Cuts at: 1402 1402
Size: 4544
PshAI GACnn'nnGTC
Cuts at: 337 337
Size: 4544
PspOMI G'GGCC_C
Cuts at: 217 217
Size: 4544
PstI C_TGCA'G
Cuts at: 1075 1591 2276 1075
Size: 516 685 3343
Fragments arranged by size:
3343 685 516
PvuI CG_AT'CG
Cuts at: 104 1413 104
Size: 1309 3235
PvuII CAG'CTG
Cuts at: 168 1221 1330 1969 2329 168
Size: 1053 109 639 360 2383
Fragments arranged by size:
2383 1053 639 360 109
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4544
SacI G_AGCT'C
Cuts at: 1286 1558 1286
Size: 272 4272
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4544
SapI GCTCTTCn'nnn_
Cuts at: 2567 2777 2567
Size: 210 4334
SbfI CC_TGCA'GG
Cuts at: 1591 1591
Size: 4544
ScaI AGT'ACT
Cuts at: 4061 4061
Size: 4544
SfcI C'TryA_G
Cuts at: 33 1071 1587 2272 3265 3456 33
Size: 1038 516 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 516 191
SfoI GGC'GCC
Cuts at: 1976 2224 1976
Size: 248 4296
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
SgrAI Cr'CCGG_yG
Cuts at: 1254 1254
Size: 4544
SmaI CCC'GGG
Cuts at: 143 1566 143
Size: 1423 3121
SmlI C'TyrA_G
Cuts at: 991 1699 2614 3106 3368 991
Size: 708 915 492 262 2167
Fragments arranged by size:
2167 915 708 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4544
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4044
StyI C'CwwG_G
Cuts at: 578 983 1678 2032 578
Size: 405 695 354 3090
Fragments arranged by size:
3090 695 405 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 2127 2260 2719 2928 444
Size: 1683 133 459 209 2060
Fragments arranged by size:
2060 1683 459 209 133
TatI w'GTAC_w
Cuts at: 4059 4059
Size: 4544
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1228 1260 4061 4093 1228
Size: 32 2801 32 1679
Fragments arranged by size:
2801 1679 32 32
Tth111I GACn'n_nGTC
Cuts at: 606 999 1306 2341 606
Size: 393 307 1035 2809
Fragments arranged by size:
2809 1035 393 307
XbaI T'CTAG_A
Cuts at: 1575 1575
Size: 4544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2039 2039
Size: 4544
XmaI C'CCGG_G
Cuts at: 141 1564 141
Size: 1423 3121
XmnI GAAnn'nnTTC
Cuts at: 1531 1531
Size: 4544
ZraI GAC'GTC
Cuts at: 450 1134 1436 450
Size: 684 302 3558
Fragments arranged by size:
3558 684 302
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsrGI BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI