Restriction map for
pF1KB9868
(Circular) (Six-base) MAPSORT of: pF1KB9868.seq Check: 6259 from: 1 to: 4463
pF1KB9868 4463 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 189 189
Size: 4463
AccI GT'mk_AC
Cuts at: 752 1501 752
Size: 749 3714
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 2288 2720 3467 3735 556
Size: 1732 432 747 268 1284
Fragments arranged by size:
1732 1284 747 432 268
AfeI AGC'GCT
Cuts at: 1219 1219
Size: 4463
AflIII A'CryG_T
Cuts at: 1815 2919 3896 1815
Size: 1104 977 2382
Fragments arranged by size:
2382 1104 977
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AleI CACnn'nnGTG
Cuts at: 790 1228 790
Size: 438 4025
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 808 808
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 1768 3335 1768
Size: 1567 2896
ApaI G_GGCC'C
Cuts at: 871 889 1031 871
Size: 18 142 4303
Fragments arranged by size:
4303 142 18
ApaLI G'TGCA_C
Cuts at: 472 3233 472
Size: 2761 1702
ApoI r'AATT_y
Cuts at: 1333 1467 3816 4072 1333
Size: 134 2349 256 1724
Fragments arranged by size:
2349 1724 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 176 871 888 1039 1052 1070 1483 176
Size: 695 17 151 13 18 413 3156
Fragments arranged by size:
3156 695 413 151 18 17 13
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 159 172 303 841 931 1479 1893 2141 2176
Size: 13 131 538 90 548 414 248 35
Cuts at: 2176 159
Size: 2446
Fragments arranged by size:
2446 548 538 414 248 131 90 35
13
BanII G_rGCy'C
Cuts at: 115 871 889 947 997 1031 1477 2507 115
Size: 756 18 58 50 34 446 1030 2071
Fragments arranged by size:
2071 1030 756 446 58 50 34 18
BbeI G_GCGC'C
Cuts at: 163 176 307 935 1897 2145 163
Size: 13 131 628 962 248 2481
Fragments arranged by size:
2481 962 628 248 131 13
BbsI GAAGACnn'nnnn_
Cuts at: 992 992
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 246 280 246
Size: 34 4429
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 227 893 957 1026 227
Size: 666 64 69 3664
Fragments arranged by size:
3664 666 69 64
BglII A'GATC_T
Cuts at: 428 1978 4015 428
Size: 1550 2037 876
Fragments arranged by size:
2037 1550 876
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 476 844 871 889 1031 2088 2181 3237 476
Size: 368 27 18 142 1057 93 1056 1702
Fragments arranged by size:
1702 1057 1056 368 142 93 27 18
BmgBI CAC'GTC
Cuts at: 447 461 447
Size: 14 4449
BmrI ACTGGGnnnn_n'
Cuts at: 1675 1756 2091 4235 1675
Size: 81 335 2144 1903
Fragments arranged by size:
2144 1903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1011 2830 1011
Size: 1819 2644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1007 1006
Size: 1 4462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1639 2518 3010 3308 283
Size: 1356 879 492 298 1438
Fragments arranged by size:
1438 1356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4167 4167
Size: 4463
BsaAI yAC'GTr
Cuts at: 756 1816 756
Size: 1060 3403
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 160 173 304 818 932 1138 1183 1894 2142
Size: 13 131 514 114 206 45 711 248
Cuts at: 2142 2844 4340 160
Size: 702 1496 283
Fragments arranged by size:
1496 711 702 514 283 248 206 131
114 45 13
BsaWI w'CCGG_w
Cuts at: 813 1652 2173 2851 3125 3272 3683 813
Size: 839 521 678 274 147 411 1593
Fragments arranged by size:
1593 839 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 982 1012 1241 1271 982
Size: 30 229 30 4174
Fragments arranged by size:
4174 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1003 1174 1003
Size: 171 4292
BseYI C'CCAG_C
Cuts at: 799 920 1888 2646 3223 3719 799
Size: 121 968 758 577 496 1543
Fragments arranged by size:
1543 968 758 577 496 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 441
Size: 4463
BsiEI CG_ry'CG
Cuts at: 104 341 593 659 956 1301 2005 2051 3259
Size: 237 252 66 297 345 704 46 1208
Cuts at: 3259 104
Size: 1308
Fragments arranged by size:
1308 1208 704 345 297 252 237 66
46
BsiHKAI G_wGCw'C
Cuts at: 266 452 476 997 1312 1477 2255 2445 3237
Size: 186 24 521 315 165 778 190 792
Cuts at: 3237 266
Size: 1492
Fragments arranged by size:
1492 792 778 521 315 190 186 165
24
Bsp1286I G_dGCh'C
Cuts at: 115 266 452 476 844 871 889 947 997
Size: 151 186 24 368 27 18 58 50
Cuts at: 997 1031 1312 1477 2088 2181 2255 2445 2507
Size: 34 281 165 611 93 74 190 62
Cuts at: 2507 3237 115
Size: 730 1341
Fragments arranged by size:
1341 730 611 368 281 190 186 165
151 93 74 62 58 50 34 27
24 18
BspEI T'CCGG_A
Cuts at: 813 3683 813
Size: 2870 1593
BspHI T'CATG_A
Cuts at: 740 3639 740
Size: 2899 1564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 189 1513 1727 2029 2410 189
Size: 1324 214 302 381 2242
Fragments arranged by size:
2242 1324 381 302 214
BsrBI CCG'CTC
Cuts at: 50 612 1017 2755 2809 4293 4458 50
Size: 562 405 1738 54 1484 165 55
Fragments arranged by size:
1738 1484 562 405 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 226 654 951 1140 2461 2642 2851 226
Size: 428 297 189 1321 181 209 1838
Fragments arranged by size:
1838 1321 428 297 209 189 181
BssHII G'CGCG_C
Cuts at: 293 293
Size: 4463
BssSI C'ACGA_G
Cuts at: 285 2734 3092 285
Size: 2449 358 1656
Fragments arranged by size:
2449 1656 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstXI CCAn_nnnn'nTGG
Cuts at: 492 864 492
Size: 372 4091
BstYI r'GATC_y
Cuts at: 1 428 1424 1488 1955 1978 2313 2559 3571
Size: 427 996 64 467 23 335 246 1012
Cuts at: 3571 3657 3669 4015 1
Size: 86 12 346 449
Fragments arranged by size:
1012 996 467 449 427 346 335 246
86 64 23 12
BtgI C'CryG_G
Cuts at: 107 1105 1238 107
Size: 998 133 3332
Fragments arranged by size:
3332 998 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 258 2413 2594 2757 258
Size: 2155 181 163 1964
Fragments arranged by size:
2155 1964 181 163
BtsI GCAGTG_nn'
Cuts at: 1706 1706
Size: 4463
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DraIII CAC_nnn'GTG
Cuts at: 578 578
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 224 338 353 380 590 656 953 1235 1298
Size: 114 15 27 210 66 297 282 63
Cuts at: 1298 2048 2222 2613 2640 3985 224
Size: 750 174 391 27 1345 702
Fragments arranged by size:
1345 750 702 391 297 282 210 174
114 66 63 27 27 15
EagI C'GGCC_G
Cuts at: 338 590 656 953 1298 2048 338
Size: 252 66 297 345 750 2753
Fragments arranged by size:
2753 750 345 297 252 66
EarI CTCTTCn'nnn_
Cuts at: 322 457 1333 2486 2696 322
Size: 135 876 1153 210 2089
Fragments arranged by size:
2089 1153 876 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1396 2671 2981 3127 4273 1396
Size: 1275 310 146 1146 1586
Fragments arranged by size:
1586 1275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 995 1475 995
Size: 480 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1006 1007 2288 2720 3467 3735 556
Size: 450 1 1281 432 747 268 1284
Fragments arranged by size:
1284 1281 747 450 432 268 1
EcoO109I rG'GnC_Cy
Cuts at: 868 1027 1028 1602 868
Size: 159 1 574 3729
Fragments arranged by size:
3729 574 159 1
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FseI GG_CCGG'CC
Cuts at: 230 230
Size: 4463
FspI TGC'GCA
Cuts at: 760 2244 760
Size: 1484 2979
FspAI rTGC'GCAy
Cuts at: 760 760
Size: 4463
HaeII r_GCGC'y
Cuts at: 163 176 307 442 935 1221 1897 2145 3167
Size: 13 131 135 493 286 676 248 1022
Cuts at: 3167 4146 163
Size: 979 480
Fragments arranged by size:
1022 979 676 493 480 286 248 135
131 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 982 1014 1416 1448 2305 2335 2337 2367 982
Size: 32 402 32 857 30 2 30 3078
Fragments arranged by size:
3078 857 402 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 159 172 303 931 1893 2141 159
Size: 13 131 628 962 248 2481
Fragments arranged by size:
2481 962 628 248 131 13
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1028 3134 3318 1028
Size: 2106 184 2173
Fragments arranged by size:
2173 2106 184
MscI TGG'CCA
Cuts at: 355 382 2224 355
Size: 27 1842 2594
Fragments arranged by size:
2594 1842 27
MslI CAynn'nnrTG
Cuts at: 277 349 574 790 1228 1249 2452 2579 277
Size: 72 225 216 438 21 1203 127 2161
Fragments arranged by size:
2161 1203 438 225 216 127 72 21
MspA1I CmG'CkG
Cuts at: 912 1107 1574 1888 2248 3261 3506 912
Size: 195 467 314 360 1013 245 1869
Fragments arranged by size:
1869 1013 467 360 314 245 195
NaeI GCC'GGC
Cuts at: 228 656 953 1142 2644 228
Size: 428 297 189 1502 2047
Fragments arranged by size:
2047 1502 428 297 189
NarI GG'CG_CC
Cuts at: 160 173 304 932 1894 2142 160
Size: 13 131 628 962 248 2481
Fragments arranged by size:
2481 962 628 248 131 13
NcoI C'CATG_G
Cuts at: 107 107
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 226 654 951 1140 2642 226
Size: 428 297 189 1502 2047
Fragments arranged by size:
2047 1502 428 297 189
NspI r_CATG'y
Cuts at: 1516 2016 2923 1516
Size: 500 907 3056
Fragments arranged by size:
3056 907 500
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PspOMI G'GGCC_C
Cuts at: 867 885 1027 867
Size: 18 142 4303
Fragments arranged by size:
4303 142 18
PstI C_TGCA'G
Cuts at: 122 200 1149 1510 2195 122
Size: 78 949 361 685 2390
Fragments arranged by size:
2390 949 685 361 78
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 1888 2248 1888
Size: 360 4103
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 997 1477 997
Size: 480 3983
SacII CC_GC'GG
Cuts at: 1108 1108
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 457 1333 2486 2696 457
Size: 876 1153 210 2224
Fragments arranged by size:
2224 1153 876 210
SbfI CC_TGCA'GG
Cuts at: 200 1510 200
Size: 1310 3153
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 118 196 1145 1506 2191 3184 3375 33
Size: 85 78 949 361 685 993 191 1121
Fragments arranged by size:
1121 993 949 685 361 191 85 78
SfiI GGCCn_nnn'nGGCC
Cuts at: 1026 1026
Size: 4463
SfoI GGC'GCC
Cuts at: 161 174 305 933 1895 2143 161
Size: 13 131 628 962 248 2481
Fragments arranged by size:
2481 962 628 248 131 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 178 890 1054 1485 178
Size: 712 164 431 3156
Fragments arranged by size:
3156 712 431 164
SmlI C'TyrA_G
Cuts at: 298 1618 2533 3025 3287 298
Size: 1320 915 492 262 1474
Fragments arranged by size:
1474 1320 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
SrfI GCCC'GGGC
Cuts at: 178 178
Size: 4463
StyI C'CwwG_G
Cuts at: 107 1178 1597 1951 107
Size: 1071 419 354 2619
Fragments arranged by size:
2619 1071 419 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 813 934 2046 2179 2638 2847 169
Size: 644 121 1112 133 459 209 1785
Fragments arranged by size:
1785 1112 644 459 209 133 121
TatI w'GTAC_w
Cuts at: 527 3978 527
Size: 3451 1012
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1241 1273 3980 4012 1241
Size: 32 2707 32 1692
Fragments arranged by size:
2707 1692 32 32
Tth111I GACn'n_nGTC
Cuts at: 820 2260 820
Size: 1440 3023
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 1958 390
Size: 1568 2895
XmaI C'CCGG_G
Cuts at: 176 888 1052 1483 176
Size: 712 164 431 3156
Fragments arranged by size:
3156 712 431 164
XmnI GAAnn'nnTTC
Cuts at: 1219 1219
Size: 4463
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SanDI SexAI SgrAI SnaBI SspI StuI SwaI XhoI
ZraI