Restriction map for
pF1KB9859
(Circular) (Six-base) MAPSORT of: pF1KB9859.seq Check: 3526 from: 1 to: 4313
pF1KB9859 4313 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 580 2138 2570 3317 3585 243
Size: 337 1558 432 747 268 971
Fragments arranged by size:
1558 971 747 432 337 268
AfeI AGC'GCT
Cuts at: 268 557 268
Size: 289 4024
AflIII A'CryG_T
Cuts at: 851 1665 2769 3746 851
Size: 814 1104 977 1418
Fragments arranged by size:
1418 1104 977 814
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 865 865
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 1008 1618 3185 1008
Size: 610 1567 2136
Fragments arranged by size:
2136 1567 610
ApaI G_GGCC'C
Cuts at: 246 246
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 200 718 772 1333 200
Size: 518 54 561 3180
Fragments arranged by size:
3180 561 518 54
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 167 899 1274 1329 1743 1991 2026 167
Size: 732 375 55 414 248 35 2454
Fragments arranged by size:
2454 732 414 375 248 55 35
BanII G_rGCy'C
Cuts at: 246 307 1327 2357 246
Size: 61 1020 1030 2202
Fragments arranged by size:
2202 1030 1020 61
BbeI G_GCGC'C
Cuts at: 171 1747 1995 171
Size: 1576 248 2489
Fragments arranged by size:
2489 1576 248
BbsI GAAGACnn'nnnn_
Cuts at: 823 823
Size: 4313
BbvCI CC'TCA_GC
Cuts at: 1073 1079 1115 1133 1073
Size: 6 36 18 4253
Fragments arranged by size:
4253 36 18 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 438 472 870 904 438
Size: 34 398 34 3847
Fragments arranged by size:
3847 398 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1166 2211 2972 4313 1166
Size: 1045 761 1341 1166
Fragments arranged by size:
1341 1166 1045 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 434 478 973 1272 434
Size: 44 495 299 3475
Fragments arranged by size:
3475 495 299 44
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 246 257 904 1277 1938 2031 3087 246
Size: 11 647 373 661 93 1056 1472
Fragments arranged by size:
1472 1056 661 647 373 93 11
BmrI ACTGGGnnnn_n'
Cuts at: 743 1525 1606 1941 4085 743
Size: 782 81 335 2144 971
Fragments arranged by size:
2144 971 782 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 595 1039 556
Size: 39 444 3830
Fragments arranged by size:
3830 444 39
Bpu10I CC'TnA_GC
Cuts at: 1073 1079 1115 1133 1073
Size: 6 36 18 4253
Fragments arranged by size:
4253 36 18 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1489 2368 2860 3158 775
Size: 714 879 492 298 1930
Fragments arranged by size:
1930 879 714 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 168 1744 1992 2694 4190 168
Size: 1576 248 702 1496 291
Fragments arranged by size:
1576 1496 702 291 248
BsaWI w'CCGG_w
Cuts at: 1502 2023 2701 2975 3122 3533 1502
Size: 521 678 274 147 411 2282
Fragments arranged by size:
2282 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 385
Size: 4313
BseYI C'CCAG_C
Cuts at: 144 189 431 523 1067 1091 1109 1139 1738
Size: 45 242 92 544 24 18 30 599
Cuts at: 1738 2496 3073 3569 144
Size: 758 577 496 888
Fragments arranged by size:
888 758 599 577 544 496 242 92
45 30 24 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 967 784
Size: 183 4130
BsiEI CG_ry'CG
Cuts at: 104 420 734 1855 1901 3109 104
Size: 316 314 1121 46 1208 1308
Fragments arranged by size:
1308 1208 1121 316 314 46
BsiHKAI G_wGCw'C
Cuts at: 307 1327 2105 2295 3087 307
Size: 1020 778 190 792 1533
Fragments arranged by size:
1533 1020 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 246 257 307 904 1277 1327 1938 2031 2105
Size: 11 50 597 373 50 611 93 74
Cuts at: 2105 2295 2357 3087 246
Size: 190 62 730 1472
Fragments arranged by size:
1472 730 611 597 373 190 93 74
62 50 50 11
BspEI T'CCGG_A
Cuts at: 3533 3533
Size: 4313
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 372 2605 2659 4143 4308 50
Size: 322 2233 54 1484 165 55
Fragments arranged by size:
2233 1484 322 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 120 729 780 1277 2311 2492 2701 120
Size: 609 51 497 1034 181 209 1732
Fragments arranged by size:
1732 1034 609 497 209 181 51
BssHII G'CGCG_C
Cuts at: 401 401
Size: 4313
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 945 1690 945
Size: 745 3568
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstXI CCAn_nnnn'nTGG
Cuts at: 751 751
Size: 4313
BstYI r'GATC_y
Cuts at: 1 1338 1805 1828 2163 2409 3421 3507 3519
Size: 1337 467 23 335 246 1012 86 12
Cuts at: 3519 3865 1
Size: 346 449
Fragments arranged by size:
1337 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 157 420 821 157
Size: 263 401 3649
Fragments arranged by size:
3649 401 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 1556 1556
Size: 4313
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 336 2019 2877 336
Size: 1683 858 1772
Fragments arranged by size:
1772 1683 858
EaeI y'GGCC_r
Cuts at: 247 417 731 1898 2072 2463 2490 3835 247
Size: 170 314 1167 174 391 27 1345 725
Fragments arranged by size:
1345 1167 725 391 314 174 170 27
EagI C'GGCC_G
Cuts at: 417 731 1898 417
Size: 314 1167 2832
Fragments arranged by size:
2832 1167 314
EarI CTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 2831 2977 4123 2521
Size: 310 146 1146 2711
Fragments arranged by size:
2711 1146 310 146
EcoICRI GAG'CTC
Cuts at: 305 1325 305
Size: 1020 3293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 556 580 595 1039 2138 2570 3317 3585
Size: 313 24 15 444 1099 432 747 268
Cuts at: 3585 243
Size: 971
Fragments arranged by size:
1099 971 747 444 432 313 268 24
15
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 4313
EcoO109I rG'GnC_Cy
Cuts at: 242 311 351 1452 242
Size: 69 40 1101 3103
Fragments arranged by size:
3103 1101 69 40
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FseI GG_CCGG'CC
Cuts at: 733 733
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 135 171 234 270 559 1747 1995 3017 3996
Size: 36 63 36 289 1188 248 1022 979
Cuts at: 3996 135
Size: 452
Fragments arranged by size:
1188 1022 979 452 289 248 63 36
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2155 2185 2187 2217 2155
Size: 30 2 30 4251
Fragments arranged by size:
4251 30 30 2
HincII GTy'rAC
Cuts at: 1352 1352
Size: 4313
KasI G'GCGC_C
Cuts at: 167 1743 1991 167
Size: 1576 248 2489
Fragments arranged by size:
2489 1576 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 1322 2984 3168 833
Size: 489 1662 184 1978
Fragments arranged by size:
1978 1662 489 184
MscI TGG'CCA
Cuts at: 2074 2074
Size: 4313
MslI CAynn'nnrTG
Cuts at: 865 2302 2429 865
Size: 1437 127 2749
Fragments arranged by size:
2749 1437 127
MspA1I CmG'CkG
Cuts at: 159 189 431 666 760 1424 1738 2098 3111
Size: 30 242 235 94 664 314 360 1013
Cuts at: 3111 3356 159
Size: 245 1116
Fragments arranged by size:
1116 1013 664 360 314 245 242 235
94 30
NaeI GCC'GGC
Cuts at: 122 731 2494 122
Size: 609 1763 1941
Fragments arranged by size:
1941 1763 609
NarI GG'CG_CC
Cuts at: 168 1744 1992 168
Size: 1576 248 2489
Fragments arranged by size:
2489 1576 248
NgoMIV G'CCGG_C
Cuts at: 120 729 2492 120
Size: 609 1763 1941
Fragments arranged by size:
1941 1763 609
NspI r_CATG'y
Cuts at: 855 1366 1866 2773 855
Size: 511 500 907 2395
Fragments arranged by size:
2395 907 511 500
PciI A'CATG_T
Cuts at: 851 2769 851
Size: 1918 2395
PfoI T'CCnGG_A
Cuts at: 307 307
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 311 351 311
Size: 40 4273
PspOMI G'GGCC_C
Cuts at: 242 242
Size: 4313
PspXI vC'TCGA_Gb
Cuts at: 772 772
Size: 4313
PstI C_TGCA'G
Cuts at: 941 1360 2045 941
Size: 419 685 3209
Fragments arranged by size:
3209 685 419
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 666 1738 2098 666
Size: 1072 360 2881
Fragments arranged by size:
2881 1072 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 307 1327 307
Size: 1020 3293
SacII CC_GC'GG
Cuts at: 160 160
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SanDI GG'GwC_CC
Cuts at: 351 351
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SexAI A'CCwGG_T
Cuts at: 1007 1007
Size: 4313
SfcI C'TryA_G
Cuts at: 33 937 1356 2041 3034 3225 33
Size: 904 419 685 993 191 1121
Fragments arranged by size:
1121 993 904 685 419 191
SfoI GGC'GCC
Cuts at: 169 1745 1993 169
Size: 1576 248 2489
Fragments arranged by size:
2489 1576 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 720 1335 720
Size: 615 3698
SmlI C'TyrA_G
Cuts at: 772 790 1468 2383 2875 3137 772
Size: 18 678 915 492 262 1948
Fragments arranged by size:
1948 915 678 492 262 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
SrfI GCCC'GGGC
Cuts at: 720 720
Size: 4313
StyI C'CwwG_G
Cuts at: 1447 1801 1447
Size: 354 3959
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 181 1214 1256 1262 1896 2029 2488 2697 181
Size: 1033 42 6 634 133 459 209 1797
Fragments arranged by size:
1797 1033 634 459 209 133 42 6
TatI w'GTAC_w
Cuts at: 848 854 950 3828 848
Size: 6 96 2878 1333
Fragments arranged by size:
2878 1333 96 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XhoI C'TCGA_G
Cuts at: 772 772
Size: 4313
XmaI C'CCGG_G
Cuts at: 718 1333 718
Size: 615 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PsrI SfiI SgrAI SnaBI SspI StuI SwaI XmnI
ZraI