Restriction map for
pF1KB9855
(Circular) (Six-base) MAPSORT of: pF1KB9855.seq Check: 4424 from: 1 to: 4283
pF1KB9855 4283 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 750 750
Size: 4283
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 182 1299 182
Size: 1117 3166
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 2108 2540 3287 3555 639
Size: 1469 432 747 268 1367
Fragments arranged by size:
1469 1367 747 432 268
AfeI AGC'GCT
Cuts at: 1048 1048
Size: 4283
AflIII A'CryG_T
Cuts at: 281 708 1635 2739 3716 281
Size: 427 927 1104 977 848
Fragments arranged by size:
1104 977 927 848 427
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AleI CACnn'nnGTG
Cuts at: 295 295
Size: 4283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaI G_GGCC'C
Cuts at: 207 1208 207
Size: 1001 3282
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 133 784 949 1303 133
Size: 651 165 354 3113
Fragments arranged by size:
3113 651 354 165
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 182 798 918 1299 1713 1961 1996 182
Size: 616 120 381 414 248 35 2469
Fragments arranged by size:
2469 616 414 381 248 120 35
BanII G_rGCy'C
Cuts at: 179 207 434 578 1208 1297 2327 179
Size: 28 227 144 630 89 1030 2135
Fragments arranged by size:
2135 1030 630 227 144 89 28
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 622 622
Size: 4283
BbvCI CC'TCA_GC
Cuts at: 1067 1194 1067
Size: 127 4156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 406 440 90
Size: 34 282 34 3933
Fragments arranged by size:
3933 282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 581 734 872 1803 581
Size: 153 138 931 3061
Fragments arranged by size:
3061 931 153 138
BglI GCCn_nnn'nGGC
Cuts at: 788 788
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 428 1395 428
Size: 967 3316
Bme1580I G_kGCm'C
Cuts at: 192 207 424 535 803 1058 1127 1208 1229
Size: 15 217 111 268 255 69 81 21
Cuts at: 1229 1908 2001 3057 192
Size: 679 93 1056 1418
Fragments arranged by size:
1418 1056 679 268 255 217 111 93
81 69 21 15
BmgBI CAC'GTC
Cuts at: 282 282
Size: 4283
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1247 1247
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 1067 1194 1067
Size: 127 4156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 1459 2338 2830 3128 289
Size: 1170 879 492 298 1444
Fragments arranged by size:
1444 1170 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 637 3987 637
Size: 3350 933
BsaAI yAC'GTr
Cuts at: 144 709 1636 144
Size: 565 927 2791
Fragments arranged by size:
2791 927 565
BsaBI GATnn'nnATC
Cuts at: 6 553 1797 3834 6
Size: 547 1244 2037 455
Fragments arranged by size:
2037 1244 547 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 1084 541
Size: 543 3740
BseYI C'CCAG_C
Cuts at: 412 922 1708 2466 3043 3539 412
Size: 510 786 758 577 496 1156
Fragments arranged by size:
1156 786 758 577 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 580 916 1122 369
Size: 211 336 206 3530
Fragments arranged by size:
3530 336 211 206
BsiEI CG_ry'CG
Cuts at: 104 690 1825 1871 3079 104
Size: 586 1135 46 1208 1308
Fragments arranged by size:
1308 1208 1135 586 46
BsiHKAI G_wGCw'C
Cuts at: 1297 2075 2265 3057 1297
Size: 778 190 792 2523
Fragments arranged by size:
2523 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 179 192 207 424 434 535 578 803 1058
Size: 13 15 217 10 101 43 225 255
Cuts at: 1058 1127 1208 1229 1297 1908 2001 2075 2265
Size: 69 81 21 68 611 93 74 190
Cuts at: 2265 2327 3057 179
Size: 62 730 1405
Fragments arranged by size:
1405 730 611 255 225 217 190 101
93 81 74 69 68 62 43 21
15 13 10
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 578 611 3459 578
Size: 33 2848 1402
Fragments arranged by size:
2848 1402 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 750 938 1333 1547 1849 2230 750
Size: 188 395 214 302 381 2803
Fragments arranged by size:
2803 395 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 319 2195 319
Size: 1876 2407
BsrFI r'CCGG_y
Cuts at: 2281 2462 2671 2281
Size: 181 209 3893
Fragments arranged by size:
3893 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstEII G'GTnAC_C
Cuts at: 400 715 400
Size: 315 3968
BstXI CCAn_nnnn'nTGG
Cuts at: 297 297
Size: 4283
BstYI r'GATC_y
Cuts at: 1 1031 1308 1775 1798 2133 2379 3391 3477
Size: 1030 277 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1030 1012 467 449 346 335 277 246
86 23 12
BtgI C'CryG_G
Cuts at: 368 396 684 824 986 1200 1229 368
Size: 28 288 140 162 214 29 3422
Fragments arranged by size:
3422 288 214 162 140 29 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 369 2233 2414 2577 369
Size: 1864 181 163 2075
Fragments arranged by size:
2075 1864 181 163
BtsI GCAGTG_nn'
Cuts at: 422 1526 422
Size: 1104 3179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 302 337 302
Size: 35 4248
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 813 813
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 310 539 687 1022 1232 1868 2042 2433 2460
Size: 229 148 335 210 636 174 391 27
Cuts at: 2460 3805 310
Size: 1345 788
Fragments arranged by size:
1345 788 636 391 335 229 210 174
148 27
EagI C'GGCC_G
Cuts at: 687 1868 687
Size: 1181 3102
EarI CTCTTCn'nnn_
Cuts at: 367 2306 2516 367
Size: 1939 210 2134
Fragments arranged by size:
2134 1939 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 639 1247 2108 2540 3287 3555 639
Size: 608 861 432 747 268 1367
Fragments arranged by size:
1367 861 747 608 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 160 808 160
Size: 648 3635
EcoO109I rG'GnC_Cy
Cuts at: 165 203 945 1205 1422 165
Size: 38 742 260 217 3026
Fragments arranged by size:
3026 742 260 217 38
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 1213 2064 1213
Size: 851 3432
HaeII r_GCGC'y
Cuts at: 746 1050 1717 1965 2987 3966 746
Size: 304 667 248 1022 979 1063
Fragments arranged by size:
1063 1022 979 667 304 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1258 1290 2125 2155 2157 2187 1258
Size: 32 835 30 2 30 3354
Fragments arranged by size:
3354 835 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 186 1303 186
Size: 1117 3166
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2954 3138 2954
Size: 184 4099
MscI TGG'CCA
Cuts at: 312 541 1234 2044 312
Size: 229 693 810 2551
Fragments arranged by size:
2551 810 693 229
MslI CAynn'nnrTG
Cuts at: 295 610 754 760 823 1120 2272 2399 295
Size: 315 144 6 63 297 1152 127 2179
Fragments arranged by size:
2179 1152 315 297 144 127 63 6
MspA1I CmG'CkG
Cuts at: 198 353 870 1202 1394 1708 2068 3081 3326
Size: 155 517 332 192 314 360 1013 245
Cuts at: 3326 198
Size: 1155
Fragments arranged by size:
1155 1013 517 360 332 314 245 192
155
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 368 824 1229 368
Size: 456 405 3422
Fragments arranged by size:
3422 456 405
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 667 759 1336 1836 2743 667
Size: 92 577 500 907 2207
Fragments arranged by size:
2207 907 577 500 92
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PflMI CCAn_nnn'nTGG
Cuts at: 374 813 374
Size: 439 3844
PfoI T'CCnGG_A
Cuts at: 254 965 254
Size: 711 3572
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PpuMI rG'GwC_Cy
Cuts at: 165 945 165
Size: 780 3503
PspOMI G'GGCC_C
Cuts at: 203 1204 203
Size: 1001 3282
PstI C_TGCA'G
Cuts at: 278 1330 2015 278
Size: 1052 685 2546
Fragments arranged by size:
2546 1052 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 353 1708 2068 353
Size: 1355 360 2568
Fragments arranged by size:
2568 1355 360
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SacII CC_GC'GG
Cuts at: 1203 1203
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 367 2306 2516 367
Size: 1939 210 2134
Fragments arranged by size:
2134 1939 210
SbfI CC_TGCA'GG
Cuts at: 278 1330 278
Size: 1052 3231
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SexAI A'CCwGG_T
Cuts at: 809 809
Size: 4283
SfcI C'TryA_G
Cuts at: 33 274 1326 2011 3004 3195 33
Size: 241 1052 685 993 191 1121
Fragments arranged by size:
1121 1052 993 685 241 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 304 949 1438 2353 2845 3107 304
Size: 645 489 915 492 262 1480
Fragments arranged by size:
1480 915 645 492 489 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 667 1336 1836 667
Size: 669 500 3114
Fragments arranged by size:
3114 669 500
StyI C'CwwG_G
Cuts at: 368 824 1229 1235 1417 1771 368
Size: 456 405 6 182 354 2880
Fragments arranged by size:
2880 456 405 354 182 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 829 936 1015 1866 1999 2458 2667 703
Size: 126 107 79 851 133 459 209 2319
Fragments arranged by size:
2319 851 459 209 133 126 107 79
TatI w'GTAC_w
Cuts at: 3798 3798
Size: 4283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 292 324 527 559 3800 3832 292
Size: 32 203 32 3241 32 743
Fragments arranged by size:
3241 743 203 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 1778 496
Size: 1282 3001
XhoI C'TCGA_G
Cuts at: 949 949
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI