Restriction map for
pF1KB9852
(Circular) (Six-base) MAPSORT of: pF1KB9852.seq Check: 6372 from: 1 to: 4259
pF1KB9852 4259 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1297 1297
Size: 4259
Acc65I G'GTAC_C
Cuts at: 1275 1275
Size: 4259
AclI AA'CG_TT
Cuts at: 4099 4099
Size: 4259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 2516 3263 3531 2084
Size: 432 747 268 2812
Fragments arranged by size:
2812 747 432 268
AflIII A'CryG_T
Cuts at: 1611 2715 3692 1611
Size: 1104 977 2178
Fragments arranged by size:
2178 1104 977
AgeI A'CCGG_T
Cuts at: 2647 2647
Size: 4259
AhdI GACnn_n'nnGTC
Cuts at: 888 888
Size: 4259
AlwNI CAG_nnn'CTG
Cuts at: 1564 3131 1564
Size: 1567 2692
ApaLI G'TGCA_C
Cuts at: 3029 3029
Size: 4259
ApoI r'AATT_y
Cuts at: 1134 1263 3612 3868 1134
Size: 129 2349 256 1525
Fragments arranged by size:
2349 1525 256 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 4259
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
AvaI C'yCGr_G
Cuts at: 1279 1279
Size: 4259
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 364 397 364
Size: 33 4226
BamHI G'GATC_C
Cuts at: 1284 1284
Size: 4259
BanI G'GyrC_C
Cuts at: 1275 1689 1937 1972 1275
Size: 414 248 35 3562
Fragments arranged by size:
3562 414 248 35
BanII G_rGCy'C
Cuts at: 874 1273 2303 874
Size: 399 1030 2830
Fragments arranged by size:
2830 1030 399
BbeI G_GCGC'C
Cuts at: 1693 1941 1693
Size: 248 4011
BciVI GTATCCnnnnn_n'
Cuts at: 592 2157 2918 4259 592
Size: 1565 761 1341 592
Fragments arranged by size:
1565 1341 761 592
BclI T'GATC_A
Cuts at: 1779 1779
Size: 4259
BglII A'GATC_T
Cuts at: 1774 3811 1774
Size: 2037 2222
BlpI GC'TnA_GC
Cuts at: 868 1371 868
Size: 503 3756
Bme1580I G_kGCm'C
Cuts at: 1884 1977 3033 1884
Size: 93 1056 3110
Fragments arranged by size:
3110 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1044 1471 1552 1887 4031 1044
Size: 427 81 335 2144 1272
Fragments arranged by size:
2144 1272 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 188 1267 138
Size: 50 1079 3130
Fragments arranged by size:
3130 1079 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 669 1435 2314 2806 3104 430
Size: 239 766 879 492 298 1585
Fragments arranged by size:
1585 879 766 492 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 896 3963 896
Size: 3067 1192
BsaAI yAC'GTr
Cuts at: 1612 1612
Size: 4259
BsaBI GATnn'nnATC
Cuts at: 6 1773 3810 6
Size: 1767 2037 455
Fragments arranged by size:
2037 1767 455
BsaHI Gr'CG_yC
Cuts at: 1690 1938 2640 4136 1690
Size: 248 702 1496 1813
Fragments arranged by size:
1813 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1448 1969 2647 2921 3068 3479 1448
Size: 521 678 274 147 411 2228
Fragments arranged by size:
2228 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 161 191 161
Size: 30 4229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 912 944 912
Size: 32 4227
BseYI C'CCAG_C
Cuts at: 216 1684 2442 3019 3515 216
Size: 1468 758 577 496 960
Fragments arranged by size:
1468 960 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1070
Size: 4259
BsiEI CG_ry'CG
Cuts at: 104 1801 1847 3055 104
Size: 1697 46 1208 1308
Fragments arranged by size:
1697 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1273 2051 2241 3033 1273
Size: 778 190 792 2499
Fragments arranged by size:
2499 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 874 1273 1884 1977 2051 2241 2303 3033 874
Size: 399 611 93 74 190 62 730 2100
Fragments arranged by size:
2100 730 611 399 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3479 3479
Size: 4259
BspHI T'CATG_A
Cuts at: 3435 3435
Size: 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1309 1523 1825 2206 1309
Size: 214 302 381 3362
Fragments arranged by size:
3362 381 302 214
BsrBI CCG'CTC
Cuts at: 50 934 2551 2605 4089 4254 50
Size: 884 1617 54 1484 165 55
Fragments arranged by size:
1617 1484 884 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2171 2171
Size: 4259
BsrFI r'CCGG_y
Cuts at: 2257 2438 2647 2257
Size: 181 209 3869
Fragments arranged by size:
3869 209 181
BssSI C'ACGA_G
Cuts at: 2530 2888 2530
Size: 358 3901
BstAPI GCAn_nnn'nTGC
Cuts at: 1636 1636
Size: 4259
BstBI TT'CG_AA
Cuts at: 2620 2620
Size: 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 463 463
Size: 4259
BstYI r'GATC_y
Cuts at: 1 675 1284 1751 1774 2109 2355 3367 3453
Size: 674 609 467 23 335 246 1012 86
Cuts at: 3453 3465 3811 1
Size: 12 346 449
Fragments arranged by size:
1012 674 609 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 126 126
Size: 4259
BtgI C'CryG_G
Cuts at: 107 716 107
Size: 609 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2209 2390 2553 2209
Size: 181 163 3915
Fragments arranged by size:
3915 181 163
BtsI GCAGTG_nn'
Cuts at: 1502 1502
Size: 4259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 867 902 867
Size: 35 4224
DraI TTT'AAA
Cuts at: 1258 1258
Size: 4259
DrdI GACnn_nn'nnGTC
Cuts at: 1965 2823 1965
Size: 858 3401
EaeI y'GGCC_r
Cuts at: 1844 2018 2409 2436 3781 1844
Size: 174 391 27 1345 2322
Fragments arranged by size:
2322 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1844 1844
Size: 4259
EarI CTCTTCn'nnn_
Cuts at: 410 521 592 796 2282 2492 410
Size: 111 71 204 1486 210 2177
Fragments arranged by size:
2177 1486 210 204 111 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2467 2777 2923 4069 2467
Size: 310 146 1146 2657
Fragments arranged by size:
2657 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1271 1271
Size: 4259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 188 1267 2084 2516 3263 3531 138
Size: 50 1079 817 432 747 268 866
Fragments arranged by size:
1079 866 817 747 432 268 50
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 4259
EcoO109I rG'GnC_Cy
Cuts at: 342 1398 342
Size: 1056 3203
EcoRI G'AATT_C
Cuts at: 1134 1263 1134
Size: 129 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 615 2278 615
Size: 1663 2596
FspI TGC'GCA
Cuts at: 2040 2040
Size: 4259
HaeII r_GCGC'y
Cuts at: 125 1693 1941 2963 3942 125
Size: 1568 248 1022 979 442
Fragments arranged by size:
1568 1022 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 874 906 2101 2131 2133 2163 238
Size: 32 604 32 1195 30 2 30 2334
Fragments arranged by size:
2334 1195 604 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1298 1298
Size: 4259
KasI G'GCGC_C
Cuts at: 1689 1937 1689
Size: 248 4011
KpnI G_GTAC'C
Cuts at: 1279 1279
Size: 4259
MluI A'CGCG_T
Cuts at: 3692 3692
Size: 4259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2930 3114 2930
Size: 184 4075
MscI TGG'CCA
Cuts at: 2020 2020
Size: 4259
MslI CAynn'nnrTG
Cuts at: 358 742 748 2248 2375 358
Size: 384 6 1500 127 2242
Fragments arranged by size:
2242 1500 384 127 6
MspA1I CmG'CkG
Cuts at: 226 1370 1684 2044 3057 3302 226
Size: 1144 314 360 1013 245 1183
Fragments arranged by size:
1183 1144 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2440 2440
Size: 4259
NarI GG'CG_CC
Cuts at: 1690 1938 1690
Size: 248 4011
NcoI C'CATG_G
Cuts at: 107 107
Size: 4259
NgoMIV G'CCGG_C
Cuts at: 2438 2438
Size: 4259
NspI r_CATG'y
Cuts at: 483 1312 1812 2719 483
Size: 829 500 907 2023
Fragments arranged by size:
2023 907 829 500
PciI A'CATG_T
Cuts at: 2715 2715
Size: 4259
PmeI GTTT'AAAC
Cuts at: 1258 1258
Size: 4259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 4227
PpuMI rG'GwC_Cy
Cuts at: 342 342
Size: 4259
PstI C_TGCA'G
Cuts at: 350 1306 1991 350
Size: 956 685 2618
Fragments arranged by size:
2618 956 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4259
PvuII CAG'CTG
Cuts at: 1684 2044 1684
Size: 360 3899
RsrII CG'GwC_CG
Cuts at: 2454 2454
Size: 4259
SacI G_AGCT'C
Cuts at: 1273 1273
Size: 4259
SalI G'TCGA_C
Cuts at: 1296 1296
Size: 4259
SapI GCTCTTCn'nnn_
Cuts at: 521 2282 2492 521
Size: 1761 210 2288
Fragments arranged by size:
2288 1761 210
SbfI CC_TGCA'GG
Cuts at: 1306 1306
Size: 4259
ScaI AGT'ACT
Cuts at: 191 3776 191
Size: 3585 674
SexAI A'CCwGG_T
Cuts at: 282 282
Size: 4259
SfcI C'TryA_G
Cuts at: 33 346 1302 1987 2980 3171 33
Size: 313 956 685 993 191 1121
Fragments arranged by size:
1121 993 956 685 313 191
SfoI GGC'GCC
Cuts at: 1691 1939 1691
Size: 248 4011
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4259
SmaI CCC'GGG
Cuts at: 1281 1281
Size: 4259
SmlI C'TyrA_G
Cuts at: 445 648 1414 2329 2821 3083 445
Size: 203 766 915 492 262 1621
Fragments arranged by size:
1621 915 766 492 262 203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4259
SphI G_CATG'C
Cuts at: 1312 1812 1312
Size: 500 3759
StyI C'CwwG_G
Cuts at: 107 758 1393 1747 107
Size: 651 635 354 2619
Fragments arranged by size:
2619 651 635 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 1842 1975 2434 2643 100
Size: 1742 133 459 209 1716
Fragments arranged by size:
1742 1716 459 209 133
TatI w'GTAC_w
Cuts at: 189 3774 189
Size: 3585 674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 3776 3808 161
Size: 32 3583 32 612
Fragments arranged by size:
3583 612 32 32
Tth111I GACn'n_nGTC
Cuts at: 2056 2056
Size: 4259
XbaI T'CTAG_A
Cuts at: 1290 1290
Size: 4259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 856 1754 856
Size: 898 3361
XmaI C'CCGG_G
Cuts at: 1279 1279
Size: 4259
XmnI GAAnn'nnTTC
Cuts at: 990 990
Size: 4259
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI