Restriction map for
pF1KB9848
(Circular) (Six-base) MAPSORT of: pF1KB9848.seq Check: 5979 from: 1 to: 4241
pF1KB9848 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 938 938
Size: 4241
AatII G_ACGT'C
Cuts at: 913 913
Size: 4241
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 1235 4081 1235
Size: 2846 1395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 940 2066 2498 3245 3513 535
Size: 405 1126 432 747 268 1263
Fragments arranged by size:
1263 1126 747 432 405 268
AfeI AGC'GCT
Cuts at: 988 988
Size: 4241
AflIII A'CryG_T
Cuts at: 404 1593 2697 3674 404
Size: 1189 1104 977 971
Fragments arranged by size:
1189 1104 977 971
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AhdI GACnn_n'nnGTC
Cuts at: 788 788
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaI G_GGCC'C
Cuts at: 283 379 842 283
Size: 96 463 3682
Fragments arranged by size:
3682 463 96
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 1245 3594 3850 1245
Size: 2349 256 1636
Fragments arranged by size:
2349 1636 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 372 540 882 1149 1186 1261 372
Size: 168 342 267 37 75 3352
Fragments arranged by size:
3352 342 267 168 75 37
AvrII C'CTAG_G
Cuts at: 654 654
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 484 762 1036 1120 1257 1671 1919 1954 484
Size: 278 274 84 137 414 248 35 2771
Fragments arranged by size:
2771 414 278 274 248 137 84 35
BanII G_rGCy'C
Cuts at: 220 283 379 831 842 1184 1255 2285 220
Size: 63 96 452 11 342 71 1030 2176
Fragments arranged by size:
2176 1030 452 342 96 71 63 11
BbeI G_GCGC'C
Cuts at: 1124 1675 1923 1124
Size: 551 248 3442
Fragments arranged by size:
3442 551 248
BbsI GAAGACnn'nnnn_
Cuts at: 1065 1065
Size: 4241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 283 379 487 597 767 842 1866 1959 3015
Size: 96 108 110 170 75 1024 93 1056
Cuts at: 3015 283
Size: 1509
Fragments arranged by size:
1509 1056 1024 170 110 108 96 93
75
BmrI ACTGGGnnnn_n'
Cuts at: 832 1453 1534 1869 4013 832
Size: 621 81 335 2144 1060
Fragments arranged by size:
2144 1060 621 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 2608 477
Size: 2131 2110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 829 1006 275
Size: 554 177 3510
Fragments arranged by size:
3510 554 177
Bpu10I CC'TnA_GC
Cuts at: 674 774 674
Size: 100 4141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1417 2296 2788 3086 689
Size: 728 879 492 298 1844
Fragments arranged by size:
1844 879 728 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 572 3945 572
Size: 3373 868
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 910 1121 1672 1920 2622 4118 910
Size: 211 551 248 702 1496 1033
Fragments arranged by size:
1496 1033 702 551 248 211
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 223
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 315 1144 315
Size: 829 3412
BseYI C'CCAG_C
Cuts at: 276 1138 1666 2424 3001 3497 276
Size: 862 528 758 577 496 1020
Fragments arranged by size:
1020 862 758 577 528 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 1076
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 760 951 1783 1829 3037 104
Size: 656 191 832 46 1208 1308
Fragments arranged by size:
1308 1208 832 656 191 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3015 1255
Size: 778 190 792 2481
Fragments arranged by size:
2481 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 220 283 379 487 597 767 831 842 1184
Size: 63 96 108 110 170 64 11 342
Cuts at: 1184 1255 1866 1959 2033 2223 2285 3015 220
Size: 71 611 93 74 190 62 730 1446
Fragments arranged by size:
1446 730 611 342 190 170 110 108
96 93 74 71 64 63 62 11
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 346 938 1291 1505 1807 2188 191
Size: 155 592 353 214 302 381 2244
Fragments arranged by size:
2244 592 381 353 302 214 155
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 1033 1165 2239 2420 2629 1033
Size: 132 1074 181 209 2645
Fragments arranged by size:
2645 1074 209 181 132
BssHII G'CGCG_C
Cuts at: 518 518
Size: 4241
BssSI C'ACGA_G
Cuts at: 213 462 1114 2512 2870 213
Size: 249 652 1398 358 1584
Fragments arranged by size:
1584 1398 652 358 249
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 495 807 495
Size: 312 3929
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 221 221
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 1181 1484 1181
Size: 303 3938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 571 606 571
Size: 35 4206
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 534 1094 1826 2000 2391 2418 3763 534
Size: 560 732 174 391 27 1345 1012
Fragments arranged by size:
1345 1012 732 560 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 979 2264 2474 979
Size: 1285 210 2746
Fragments arranged by size:
2746 1285 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 829 2449 2759 2905 4051 829
Size: 1620 310 146 1146 1019
Fragments arranged by size:
1620 1146 1019 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 535 829 940 1006 2066 2498 3245 3513
Size: 260 294 111 66 1060 432 747 268
Cuts at: 3513 275
Size: 1003
Fragments arranged by size:
1060 1003 747 432 294 268 260 111
66
EcoO109I rG'GnC_Cy
Cuts at: 320 345 376 471 1380 320
Size: 25 31 95 909 3181
Fragments arranged by size:
3181 909 95 31 25
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 617 1081 2022 617
Size: 464 941 2836
Fragments arranged by size:
2836 941 464
FspAI rTGC'GCAy
Cuts at: 617 617
Size: 4241
HaeII r_GCGC'y
Cuts at: 157 550 990 1017 1124 1675 1923 2945 3924
Size: 393 440 27 107 551 248 1022 979
Cuts at: 3924 157
Size: 474
Fragments arranged by size:
1022 979 551 474 440 393 248 107
27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 774 806 2083 2113 2115 2145 223
Size: 32 519 32 1277 30 2 30 2319
Fragments arranged by size:
2319 1277 519 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1120 1671 1919 1120
Size: 551 248 3442
Fragments arranged by size:
3442 551 248
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 555 1256 2912 3096 555
Size: 701 1656 184 1700
Fragments arranged by size:
1700 1656 701 184
MscI TGG'CCA
Cuts at: 536 1096 2002 536
Size: 560 906 2775
Fragments arranged by size:
2775 906 560
MslI CAynn'nnrTG
Cuts at: 416 611 2230 2357 416
Size: 195 1619 127 2300
Fragments arranged by size:
2300 1619 195 127
MspA1I CmG'CkG
Cuts at: 118 246 276 491 634 944 1352 1666 2026
Size: 128 30 215 143 310 408 314 360
Cuts at: 2026 3039 3284 118
Size: 1013 245 1075
Fragments arranged by size:
1075 1013 408 360 314 310 245 215
143 128 30
NaeI GCC'GGC
Cuts at: 1167 2422 1167
Size: 1255 2986
NarI GG'CG_CC
Cuts at: 1121 1672 1920 1121
Size: 551 248 3442
Fragments arranged by size:
3442 551 248
NgoMIV G'CCGG_C
Cuts at: 1165 2420 1165
Size: 1255 2986
NspI r_CATG'y
Cuts at: 408 616 1294 1794 2701 408
Size: 208 678 500 907 1948
Fragments arranged by size:
1948 907 678 500 208
PciI A'CATG_T
Cuts at: 404 2697 404
Size: 2293 1948
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PpuMI rG'GwC_Cy
Cuts at: 345 471 345
Size: 126 4115
PspOMI G'GGCC_C
Cuts at: 279 375 838 279
Size: 96 463 3682
Fragments arranged by size:
3682 463 96
PstI C_TGCA'G
Cuts at: 188 251 266 398 1013 1066 1288 1973 188
Size: 63 15 132 615 53 222 685 2456
Fragments arranged by size:
2456 685 615 222 132 63 53 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 491 634 1666 2026 491
Size: 143 1032 360 2706
Fragments arranged by size:
2706 1032 360 143
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 979 2264 2474 979
Size: 1285 210 2746
Fragments arranged by size:
2746 1285 210
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 184 247 262 394 1009 1062 1284 1969
Size: 151 63 15 132 615 53 222 685
Cuts at: 1969 2962 3153 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 615 222 191 151 132
63 53 15
SfoI GGC'GCC
Cuts at: 1122 1673 1921 1122
Size: 551 248 3442
Fragments arranged by size:
3442 551 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 374 542 884 1188 1263 374
Size: 168 342 304 75 3352
Fragments arranged by size:
3352 342 304 168 75
SmlI C'TyrA_G
Cuts at: 704 1396 2311 2803 3065 704
Size: 692 915 492 262 1880
Fragments arranged by size:
1880 915 692 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 616 1294 1794 616
Size: 678 500 3063
Fragments arranged by size:
3063 678 500
StuI AGG'CCT
Cuts at: 679 679
Size: 4241
StyI C'CwwG_G
Cuts at: 365 654 680 1124 1375 1729 365
Size: 289 26 444 251 354 2877
Fragments arranged by size:
2877 444 354 289 251 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 458 1824 1957 2416 2625 458
Size: 1366 133 459 209 2074
Fragments arranged by size:
2074 1366 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 3758 3790 448
Size: 32 3278 32 899
Fragments arranged by size:
3278 899 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 372 540 882 1186 1261 372
Size: 168 342 304 75 3352
Fragments arranged by size:
3352 342 304 168 75
ZraI GAC'GTC
Cuts at: 911 911
Size: 4241
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AleI AlfI AloI AscI BaeI BbvCI BfrBI
BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I
BtgI ClaI DraIII EcoNI EcoRV FseI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI