Restriction map for
pF1KB9843
(Circular) (Six-base) MAPSORT of: pF1KB9843.seq Check: 8479 from: 1 to: 4211
pF1KB9843 4211 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 1103 2036 2468 3215 3483 327
Size: 776 933 432 747 268 1055
Fragments arranged by size:
1055 933 776 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AlwNI CAG_nnn'CTG
Cuts at: 490 1516 3083 490
Size: 1026 1567 1618
Fragments arranged by size:
1618 1567 1026
ApaLI G'TGCA_C
Cuts at: 2981 2981
Size: 4211
ApoI r'AATT_y
Cuts at: 120 176 887 919 1076 1215 3564 3820 120
Size: 56 711 32 157 139 2349 256 511
Fragments arranged by size:
2349 711 511 256 157 139 56 32
AseI AT'TA_AT
Cuts at: 20 997 20
Size: 977 3234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 1231 1231
Size: 4211
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 593 1227 1641 1889 1924 593
Size: 634 414 248 35 2880
Fragments arranged by size:
2880 634 414 248 35
BanII G_rGCy'C
Cuts at: 1225 2255 1225
Size: 1030 3181
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 1169 1169
Size: 4211
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 129 1731 129
Size: 1602 2609
BfrBI ATG'CAT
Cuts at: 623 623
Size: 4211
BglII A'GATC_T
Cuts at: 953 1726 3763 953
Size: 773 2037 1401
Fragments arranged by size:
2037 1401 773
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 1836 1929 2985 1836
Size: 93 1056 3062
Fragments arranged by size:
3062 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1423 1504 1839 3983 1423
Size: 81 335 2144 1651
Fragments arranged by size:
2144 1651 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 609
Size: 4211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3915 3915
Size: 4211
BsaAI yAC'GTr
Cuts at: 431 1564 431
Size: 1133 3078
BsaBI GATnn'nnATC
Cuts at: 6 470 700 1725 3762 6
Size: 464 230 1025 2037 455
Fragments arranged by size:
2037 1025 464 455 230
BsaHI Gr'CG_yC
Cuts at: 1642 1890 2592 4088 1642
Size: 248 702 1496 1765
Fragments arranged by size:
1765 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1400 1921 2599 2873 3020 3431 1400
Size: 521 678 274 147 411 2180
Fragments arranged by size:
2180 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 569 790 569
Size: 221 3990
BseYI C'CCAG_C
Cuts at: 1636 2394 2971 3467 1636
Size: 758 577 496 2380
Fragments arranged by size:
2380 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 411 253
Size: 158 4053
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 436 1225 2003 2193 2985 436
Size: 789 778 190 792 1662
Fragments arranged by size:
1662 792 789 778 190
BsmI GAATG_Cn'
Cuts at: 170 625 1177 170
Size: 455 552 3204
Fragments arranged by size:
3204 552 455
Bsp1286I G_dGCh'C
Cuts at: 436 1225 1836 1929 2003 2193 2255 2985 436
Size: 789 611 93 74 190 62 730 1662
Fragments arranged by size:
1662 789 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 430 1261 1475 1777 2158 430
Size: 831 214 302 381 2483
Fragments arranged by size:
2483 831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2503 2557 4041 4206 50
Size: 2453 54 1484 165 55
Fragments arranged by size:
2453 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2123 2123
Size: 4211
BsrFI r'CCGG_y
Cuts at: 2209 2390 2599 2209
Size: 181 209 3821
Fragments arranged by size:
3821 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 1588 1588
Size: 4211
BstBI TT'CG_AA
Cuts at: 2572 2572
Size: 4211
BstXI CCAn_nnnn'nTGG
Cuts at: 180 990 1101 180
Size: 810 111 3290
Fragments arranged by size:
3290 810 111
BstYI r'GATC_y
Cuts at: 1 953 1236 1703 1726 2061 2307 3319 3405
Size: 952 283 467 23 335 246 1012 86
Cuts at: 3405 3417 3763 1
Size: 12 346 449
Fragments arranged by size:
1012 952 467 449 346 335 283 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1209 2161 2342 2505 1209
Size: 952 181 163 2915
Fragments arranged by size:
2915 952 181 163
BtsI GCAGTG_nn'
Cuts at: 1454 1454
Size: 4211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 674 709 674
Size: 35 4176
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 786 1796 1970 2361 2388 3733 786
Size: 1010 174 391 27 1345 1264
Fragments arranged by size:
1345 1264 1010 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2419 2729 2875 4021 2419
Size: 310 146 1146 2609
Fragments arranged by size:
2609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1223 1223
Size: 4211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 609 1103 2036 2468 3215 3483 327
Size: 282 494 933 432 747 268 1055
Fragments arranged by size:
1055 933 747 494 432 282 268
EcoO109I rG'GnC_Cy
Cuts at: 1350 1350
Size: 4211
EcoRI G'AATT_C
Cuts at: 919 1215 919
Size: 296 3915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 4211
FspI TGC'GCA
Cuts at: 1992 1992
Size: 4211
HaeII r_GCGC'y
Cuts at: 1645 1893 2915 3894 1645
Size: 248 1022 979 1962
Fragments arranged by size:
1962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2053 2083 2085 2115 2053
Size: 30 2 30 4149
Fragments arranged by size:
4149 30 30 2
HincII GTy'rAC
Cuts at: 414 1250 414
Size: 836 3375
HindIII A'AGCT_T
Cuts at: 1186 1186
Size: 4211
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2882 3066 2882
Size: 184 4027
MscI TGG'CCA
Cuts at: 788 1972 788
Size: 1184 3027
MslI CAynn'nnrTG
Cuts at: 2200 2327 2200
Size: 127 4084
MspA1I CmG'CkG
Cuts at: 1322 1636 1996 3009 3254 1322
Size: 314 360 1013 245 2279
Fragments arranged by size:
2279 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 4211
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NcoI C'CATG_G
Cuts at: 107 107
Size: 4211
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 4211
NsiI A_TGCA'T
Cuts at: 625 625
Size: 4211
NspI r_CATG'y
Cuts at: 526 1264 1764 2671 526
Size: 738 500 907 2066
Fragments arranged by size:
2066 907 738 500
PasI CC'CwG_GG
Cuts at: 990 990
Size: 4211
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PmlI CAC'GTG
Cuts at: 431 431
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PsiI TTA'TAA
Cuts at: 1132 1132
Size: 4211
PstI C_TGCA'G
Cuts at: 1258 1943 1258
Size: 685 3526
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 1636 1996 1636
Size: 360 3851
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1225 1225
Size: 4211
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SfcI C'TryA_G
Cuts at: 33 455 1254 1939 2932 3123 33
Size: 422 799 685 993 191 1121
Fragments arranged by size:
1121 993 799 685 422 191
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1233 1233
Size: 4211
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 526 1264 1764 526
Size: 738 500 2973
Fragments arranged by size:
2973 738 500
StyI C'CwwG_G
Cuts at: 107 597 1345 1699 107
Size: 490 748 354 2619
Fragments arranged by size:
2619 748 490 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 994 1794 1927 2386 2595 994
Size: 800 133 459 209 2610
Fragments arranged by size:
2610 800 459 209 133
TatI w'GTAC_w
Cuts at: 843 3726 843
Size: 2883 1328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 3728 3760 631
Size: 32 3065 32 1082
Fragments arranged by size:
3065 1082 32 32
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1706 1706
Size: 4211
XmaI C'CCGG_G
Cuts at: 1231 1231
Size: 4211
XmnI GAAnn'nnTTC
Cuts at: 1177 1177
Size: 4211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PfoI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI