Restriction map for
pF1KB9840
(Circular) (Six-base) MAPSORT of: pF1KB9840.seq Check: 9761 from: 1 to: 4181
pF1KB9840 4181 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1219 1219
Size: 4181
Acc65I G'GTAC_C
Cuts at: 1197 1197
Size: 4181
AclI AA'CG_TT
Cuts at: 4021 4021
Size: 4181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 2438 3185 3453 2006
Size: 432 747 268 2734
Fragments arranged by size:
2734 747 432 268
AflIII A'CryG_T
Cuts at: 1533 2637 3614 1533
Size: 1104 977 2100
Fragments arranged by size:
2100 1104 977
AgeI A'CCGG_T
Cuts at: 2569 2569
Size: 4181
AlwNI CAG_nnn'CTG
Cuts at: 1486 3053 1486
Size: 1567 2614
ApaI G_GGCC'C
Cuts at: 1041 1041
Size: 4181
ApaLI G'TGCA_C
Cuts at: 2951 2951
Size: 4181
ApoI r'AATT_y
Cuts at: 1185 3534 3790 1185
Size: 2349 256 1576
Fragments arranged by size:
2349 1576 256
AseI AT'TA_AT
Cuts at: 20 1013 20
Size: 993 3188
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
AvaI C'yCGr_G
Cuts at: 1201 1201
Size: 4181
BamHI G'GATC_C
Cuts at: 221 1206 221
Size: 985 3196
BanI G'GyrC_C
Cuts at: 417 995 1003 1197 1611 1859 1894 417
Size: 578 8 194 414 248 35 2704
Fragments arranged by size:
2704 578 414 248 194 35 8
BanII G_rGCy'C
Cuts at: 1041 1195 2225 1041
Size: 154 1030 2997
Fragments arranged by size:
2997 1030 154
BbeI G_GCGC'C
Cuts at: 999 1007 1615 1863 999
Size: 8 608 248 3317
Fragments arranged by size:
3317 608 248 8
BciVI GTATCCnnnnn_n'
Cuts at: 2079 2840 4181 2079
Size: 761 1341 2079
Fragments arranged by size:
2079 1341 761
BclI T'GATC_A
Cuts at: 329 1701 329
Size: 1372 2809
BfrBI ATG'CAT
Cuts at: 934 934
Size: 4181
BglII A'GATC_T
Cuts at: 1696 3733 1696
Size: 2037 2144
BlpI GC'TnA_GC
Cuts at: 1293 1293
Size: 4181
Bme1580I G_kGCm'C
Cuts at: 1041 1806 1899 2955 1041
Size: 765 93 1056 2267
Fragments arranged by size:
2267 1056 765 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 615 673 1054 1119 1393 1474 1809 3953
Size: 192 58 381 65 274 81 335 2144
Cuts at: 3953 423
Size: 651
Fragments arranged by size:
2144 651 381 335 274 192 81 65
58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 633 2548 633
Size: 1915 2266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 628 769 876 884 364
Size: 264 141 107 8 3661
Fragments arranged by size:
3661 264 141 107 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1357 2236 2728 3026 1357
Size: 879 492 298 2512
Fragments arranged by size:
2512 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3885 3885
Size: 4181
BsaAI yAC'GTr
Cuts at: 1534 1534
Size: 4181
BsaBI GATnn'nnATC
Cuts at: 6 250 1695 3732 6
Size: 244 1445 2037 455
Fragments arranged by size:
2037 1445 455 244
BsaHI Gr'CG_yC
Cuts at: 996 1004 1612 1860 2562 4058 996
Size: 8 608 248 702 1496 1119
Fragments arranged by size:
1496 1119 702 608 248 8
BsaWI w'CCGG_w
Cuts at: 270 871 1370 1891 2569 2843 2990 3401 270
Size: 601 499 521 678 274 147 411 1050
Fragments arranged by size:
1050 678 601 521 499 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 783 813 783
Size: 30 4151
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 1062 244
Size: 818 3363
BseYI C'CCAG_C
Cuts at: 1606 2364 2941 3437 1606
Size: 758 577 496 2350
Fragments arranged by size:
2350 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 1191 729
Size: 462 3719
BsiEI CG_ry'CG
Cuts at: 104 1723 1769 2977 104
Size: 1619 46 1208 1308
Fragments arranged by size:
1619 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1195 1973 2163 2955 1195
Size: 778 190 792 2421
Fragments arranged by size:
2421 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 604 604
Size: 4181
Bsp1286I G_dGCh'C
Cuts at: 1041 1195 1806 1899 1973 2163 2225 2955 1041
Size: 154 611 93 74 190 62 730 2267
Fragments arranged by size:
2267 730 611 190 154 93 74 62
BspEI T'CCGG_A
Cuts at: 270 3401 270
Size: 3131 1050
BspHI T'CATG_A
Cuts at: 641 3357 641
Size: 2716 1465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 486 1074 1231 1445 1747 2128 486
Size: 588 157 214 302 381 2539
Fragments arranged by size:
2539 588 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 537 804 2473 2527 4011 4176 50
Size: 487 267 1669 54 1484 165 55
Fragments arranged by size:
1669 1484 487 267 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2093 2093
Size: 4181
BsrFI r'CCGG_y
Cuts at: 115 292 420 666 2179 2360 2569 115
Size: 177 128 246 1513 181 209 1727
Fragments arranged by size:
1727 1513 246 209 181 177 128
BssHII G'CGCG_C
Cuts at: 239 239
Size: 4181
BssSI C'ACGA_G
Cuts at: 2452 2810 2452
Size: 358 3823
BstAPI GCAn_nnn'nTGC
Cuts at: 1169 1558 1169
Size: 389 3792
BstBI TT'CG_AA
Cuts at: 2542 2542
Size: 4181
BstEII G'GTnAC_C
Cuts at: 399 399
Size: 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 1062 1062
Size: 4181
BstYI r'GATC_y
Cuts at: 1 221 473 1019 1206 1673 1696 2031 2277
Size: 220 252 546 187 467 23 335 246
Cuts at: 2277 3289 3375 3387 3733 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 546 467 449 346 335 252 246
220 187 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 406 406
Size: 4181
BtgI C'CryG_G
Cuts at: 107 380 107
Size: 273 3908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 534 2131 2312 2475 534
Size: 1597 181 163 2240
Fragments arranged by size:
2240 1597 181 163
BtsI GCAGTG_nn'
Cuts at: 1424 1424
Size: 4181
DraI TTT'AAA
Cuts at: 1180 1180
Size: 4181
DraIII CAC_nnn'GTG
Cuts at: 431 578 431
Size: 147 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1887 2745 1887
Size: 858 3323
EaeI y'GGCC_r
Cuts at: 383 422 668 696 812 1766 1940 2331 2358
Size: 39 246 28 116 954 174 391 27
Cuts at: 2358 3703 383
Size: 1345 861
Fragments arranged by size:
1345 954 861 391 246 174 116 39
28 27
EagI C'GGCC_G
Cuts at: 1766 1766
Size: 4181
EarI CTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1012 2389 2699 2845 3991 1012
Size: 1377 310 146 1146 1202
Fragments arranged by size:
1377 1202 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1193 1193
Size: 4181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 628 769 876 884 2006 2438 3185 3453
Size: 264 141 107 8 1122 432 747 268
Cuts at: 3453 364
Size: 1092
Fragments arranged by size:
1122 1092 747 432 268 264 141 107
8
EcoNI CCTnn'n_nnAGG
Cuts at: 1077 1077
Size: 4181
EcoO109I rG'GnC_Cy
Cuts at: 392 1037 1320 392
Size: 645 283 3253
Fragments arranged by size:
3253 645 283
EcoRI G'AATT_C
Cuts at: 1185 1185
Size: 4181
EcoRV GAT'ATC
Cuts at: 354 354
Size: 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 4181
FspI TGC'GCA
Cuts at: 1962 1962
Size: 4181
HaeII r_GCGC'y
Cuts at: 999 1007 1615 1863 2885 3864 999
Size: 8 608 248 1022 979 1316
Fragments arranged by size:
1316 1022 979 608 248 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 757 789 880 912 2023 2053 2055 2085 757
Size: 32 91 32 1111 30 2 30 2853
Fragments arranged by size:
2853 1111 91 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1220 1220
Size: 4181
KasI G'GCGC_C
Cuts at: 995 1003 1611 1859 995
Size: 8 608 248 3317
Fragments arranged by size:
3317 608 248 8
KpnI G_GTAC'C
Cuts at: 1201 1201
Size: 4181
MluI A'CGCG_T
Cuts at: 3614 3614
Size: 4181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2852 3036 2852
Size: 184 3997
MscI TGG'CCA
Cuts at: 814 1942 814
Size: 1128 3053
MslI CAynn'nnrTG
Cuts at: 2170 2297 2170
Size: 127 4054
MspA1I CmG'CkG
Cuts at: 382 1292 1606 1966 2979 3224 382
Size: 910 314 360 1013 245 1339
Fragments arranged by size:
1339 1013 910 360 314 245
NaeI GCC'GGC
Cuts at: 422 668 2362 422
Size: 246 1694 2241
Fragments arranged by size:
2241 1694 246
NarI GG'CG_CC
Cuts at: 996 1004 1612 1860 996
Size: 8 608 248 3317
Fragments arranged by size:
3317 608 248 8
NcoI C'CATG_G
Cuts at: 107 107
Size: 4181
NgoMIV G'CCGG_C
Cuts at: 420 666 2360 420
Size: 246 1694 2241
Fragments arranged by size:
2241 1694 246
NsiI A_TGCA'T
Cuts at: 936 936
Size: 4181
NspI r_CATG'y
Cuts at: 1234 1734 2641 1234
Size: 500 907 2774
Fragments arranged by size:
2774 907 500
PciI A'CATG_T
Cuts at: 2637 2637
Size: 4181
PflMI CCAn_nnn'nTGG
Cuts at: 747 747
Size: 4181
PmeI GTTT'AAAC
Cuts at: 1180 1180
Size: 4181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 3320
Size: 32 4149
PshAI GACnn'nnGTC
Cuts at: 840 840
Size: 4181
PspOMI G'GGCC_C
Cuts at: 1037 1037
Size: 4181
PstI C_TGCA'G
Cuts at: 1228 1913 1228
Size: 685 3496
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4181
PvuII CAG'CTG
Cuts at: 1606 1966 1606
Size: 360 3821
RsrII CG'GwC_CG
Cuts at: 2376 2376
Size: 4181
SacI G_AGCT'C
Cuts at: 1195 1195
Size: 4181
SacII CC_GC'GG
Cuts at: 383 383
Size: 4181
SalI G'TCGA_C
Cuts at: 1218 1218
Size: 4181
SapI GCTCTTCn'nnn_
Cuts at: 2204 2414 2204
Size: 210 3971
SbfI CC_TGCA'GG
Cuts at: 1228 1228
Size: 4181
ScaI AGT'ACT
Cuts at: 3698 3698
Size: 4181
SexAI A'CCwGG_T
Cuts at: 824 824
Size: 4181
SfcI C'TryA_G
Cuts at: 33 1224 1909 2902 3093 33
Size: 1191 685 993 191 1121
Fragments arranged by size:
1191 1121 993 685 191
SfoI GGC'GCC
Cuts at: 997 1005 1613 1861 997
Size: 8 608 248 3317
Fragments arranged by size:
3317 608 248 8
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4181
SmaI CCC'GGG
Cuts at: 1203 1203
Size: 4181
SmlI C'TyrA_G
Cuts at: 1336 2251 2743 3005 1336
Size: 915 492 262 2512
Fragments arranged by size:
2512 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4181
SphI G_CATG'C
Cuts at: 1234 1734 1234
Size: 500 3681
StyI C'CwwG_G
Cuts at: 107 999 1315 1669 107
Size: 892 316 354 2619
Fragments arranged by size:
2619 892 354 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 442 754 964 1764 1897 2356 2565 442
Size: 312 210 800 133 459 209 2058
Fragments arranged by size:
2058 800 459 312 210 209 133
TatI w'GTAC_w
Cuts at: 3696 3696
Size: 4181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 125 157 783 815 3698 3730 125
Size: 32 626 32 2883 32 576
Fragments arranged by size:
2883 626 576 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1978 1978
Size: 4181
XbaI T'CTAG_A
Cuts at: 762 1212 762
Size: 450 3731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 744 1116 1676 744
Size: 372 560 3249
Fragments arranged by size:
3249 560 372
XmaI C'CCGG_G
Cuts at: 1201 1201
Size: 4181
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsrGI BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PsiI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI