Restriction map for
pF1KB9838
(Circular) (Six-base) MAPSORT of: pF1KB9838.seq Check: 6098 from: 1 to: 4169
pF1KB9838 4169 bp
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1012 1994 2426 3173 3441 828
Size: 184 982 432 747 268 1556
Fragments arranged by size:
1556 982 747 432 268 184
AfeI AGC'GCT
Cuts at: 226 781 226
Size: 555 3614
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AleI CACnn'nnGTG
Cuts at: 746 746
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 311 1474 3041 311
Size: 1163 1567 1439
Fragments arranged by size:
1567 1439 1163
ApaI G_GGCC'C
Cuts at: 409 409
Size: 4169
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 1173 3522 3778 1173
Size: 2349 256 1564
Fragments arranged by size:
2349 1564 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 214 324 388 429 490 511 612 1072 1189
Size: 110 64 41 61 21 101 460 117
Cuts at: 1189 214
Size: 3194
Fragments arranged by size:
3194 460 117 110 101 64 61 41
21
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 130 328 382 412 424 649 1007 1185 1599
Size: 198 54 30 12 225 358 178 414
Cuts at: 1599 1847 1882 130
Size: 248 35 2417
Fragments arranged by size:
2417 414 358 248 225 198 178 54
35 30 12
BanII G_rGCy'C
Cuts at: 409 1183 2213 409
Size: 774 1030 2365
Fragments arranged by size:
2365 1030 774
BbeI G_GCGC'C
Cuts at: 134 386 416 653 1011 1603 1851 134
Size: 252 30 237 358 592 248 2452
Fragments arranged by size:
2452 592 358 252 248 237 30
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglI GCCn_nnn'nGGC
Cuts at: 551 598 1067 551
Size: 47 469 3653
Fragments arranged by size:
3653 469 47
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 271 1281 271
Size: 1010 3159
Bme1580I G_kGCm'C
Cuts at: 296 331 409 427 1147 1794 1887 2943 296
Size: 35 78 18 720 647 93 1056 1522
Fragments arranged by size:
1522 1056 720 647 93 78 35 18
BmrI ACTGGGnnnn_n'
Cuts at: 1381 1462 1797 3941 1381
Size: 81 335 2144 1609
Fragments arranged by size:
2144 1609 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 801 2536 801
Size: 1735 2434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 793 955 970 1018 466
Size: 327 162 15 48 3617
Fragments arranged by size:
3617 327 162 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 874 1345 2224 2716 3014 853
Size: 21 471 879 492 298 2008
Fragments arranged by size:
2008 879 492 471 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 3873 3873
Size: 4169
BsaAI yAC'GTr
Cuts at: 765 1522 765
Size: 757 3412
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 131 265 383 413 508 650 1008 1600 1848
Size: 134 118 30 95 142 358 592 248
Cuts at: 1848 2550 4046 131
Size: 702 1496 254
Fragments arranged by size:
1496 702 592 358 254 248 142 134
118 95 30
BsaWI w'CCGG_w
Cuts at: 1358 1879 2557 2831 2978 3389 1358
Size: 521 678 274 147 411 2138
Fragments arranged by size:
2138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 439 469 439
Size: 30 4139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 276 288 790 868 276
Size: 12 502 78 3577
Fragments arranged by size:
3577 502 78 12
BseYI C'CCAG_C
Cuts at: 142 304 319 1594 2352 2929 3425 142
Size: 162 15 1275 758 577 496 886
Fragments arranged by size:
1275 886 758 577 496 162 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 952
Size: 4169
BsiEI CG_ry'CG
Cuts at: 104 584 1151 1711 1757 2965 104
Size: 480 567 560 46 1208 1308
Fragments arranged by size:
1308 1208 567 560 480 46
BsiHKAI G_wGCw'C
Cuts at: 1183 1961 2151 2943 1183
Size: 778 190 792 2409
Fragments arranged by size:
2409 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 374 374
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 296 331 409 427 1147 1183 1794 1887 1961
Size: 35 78 18 720 36 611 93 74
Cuts at: 1961 2151 2213 2943 296
Size: 190 62 730 1522
Fragments arranged by size:
1522 730 720 611 190 93 78 74
62 36 35 18
BspEI T'CCGG_A
Cuts at: 3389 3389
Size: 4169
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 270 401 467 637 1023 2461 2515 3999
Size: 220 131 66 170 386 1438 54 1484
Cuts at: 3999 4164 50
Size: 165 55
Fragments arranged by size:
1484 1438 386 220 170 165 131 66
55 54
BsrDI GCAATG_nn'
Cuts at: 2081 2081
Size: 4169
BsrFI r'CCGG_y
Cuts at: 331 438 652 827 1090 1123 2167 2348 2557
Size: 107 214 175 263 33 1044 181 209
Cuts at: 2557 331
Size: 1943
Fragments arranged by size:
1943 1044 263 214 209 181 175 107
33
BsrGI T'GTAC_A
Cuts at: 1031 1031
Size: 4169
BssHII G'CGCG_C
Cuts at: 314 554 566 668 314
Size: 240 12 102 3815
Fragments arranged by size:
3815 240 102 12
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 281 1546 281
Size: 1265 2904
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstXI CCAn_nnnn'nTGG
Cuts at: 727 727
Size: 4169
BstYI r'GATC_y
Cuts at: 1 450 1194 1661 1684 2019 2265 3277 3363
Size: 449 744 467 23 335 246 1012 86
Cuts at: 3363 3375 3721 1
Size: 12 346 449
Fragments arranged by size:
1012 744 467 449 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 113 549 561 606 663 726 837 897
Size: 6 436 12 45 57 63 111 60
Cuts at: 897 1099 107
Size: 202 3177
Fragments arranged by size:
3177 436 202 111 63 60 57 45
12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 139 2119 2300 2463 139
Size: 1980 181 163 1845
Fragments arranged by size:
1980 1845 181 163
BtsI GCAGTG_nn'
Cuts at: 1412 1412
Size: 4169
DraI TTT'AAA
Cuts at: 1168 1168
Size: 4169
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 4169
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 110 155 543 581 654 1013 1148 1754 1928
Size: 45 388 38 73 359 135 606 174
Cuts at: 1928 2319 2346 3691 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 606 588 391 388 359 174 135
73 45 38 27
EagI C'GGCC_G
Cuts at: 581 1148 1754 581
Size: 567 606 2996
Fragments arranged by size:
2996 606 567
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 647 2377 2687 2833 3979 647
Size: 1730 310 146 1146 837
Fragments arranged by size:
1730 1146 837 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 793 828 955 970 1012 1018 1994 2426
Size: 327 35 127 15 42 6 976 432
Cuts at: 2426 3173 3441 466
Size: 747 268 1194
Fragments arranged by size:
1194 976 747 432 327 268 127 42
35 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1033 1033
Size: 4169
EcoO109I rG'GnC_Cy
Cuts at: 405 406 1026 1308 405
Size: 1 620 282 3266
Fragments arranged by size:
3266 620 282 1
EcoRI G'AATT_C
Cuts at: 1173 1173
Size: 4169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 4169
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 134 228 386 416 445 499 532 653 783
Size: 94 158 30 29 54 33 121 130
Cuts at: 783 1011 1603 1851 2873 3852 134
Size: 228 592 248 1022 979 451
Fragments arranged by size:
1022 979 592 451 248 228 158 130
121 94 54 33 30 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2011 2041 2043 2073 2011
Size: 30 2 30 4107
Fragments arranged by size:
4107 30 30 2
HincII GTy'rAC
Cuts at: 171 1208 171
Size: 1037 3132
KasI G'GCGC_C
Cuts at: 130 382 412 649 1007 1599 1847 130
Size: 252 30 237 358 592 248 2452
Fragments arranged by size:
2452 592 358 252 248 237 30
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 812 2840 3024 812
Size: 2028 184 1957
Fragments arranged by size:
2028 1957 184
MscI TGG'CCA
Cuts at: 157 1015 1930 157
Size: 858 915 2396
Fragments arranged by size:
2396 915 858
MslI CAynn'nnrTG
Cuts at: 725 746 811 2158 2285 725
Size: 21 65 1347 127 2609
Fragments arranged by size:
2609 1347 127 65 21
MspA1I CmG'CkG
Cuts at: 115 153 542 608 798 839 899 939 990
Size: 38 389 66 190 41 60 40 51
Cuts at: 990 1101 1280 1594 1954 2967 3212 115
Size: 111 179 314 360 1013 245 1072
Fragments arranged by size:
1072 1013 389 360 314 245 190 179
111 66 60 51 41 40 38
NaeI GCC'GGC
Cuts at: 440 654 2350 440
Size: 214 1696 2259
Fragments arranged by size:
2259 1696 214
NarI GG'CG_CC
Cuts at: 131 383 413 650 1008 1600 1848 131
Size: 252 30 237 358 592 248 2452
Fragments arranged by size:
2452 592 358 252 248 237 30
NcoI C'CATG_G
Cuts at: 107 726 107
Size: 619 3550
NgoMIV G'CCGG_C
Cuts at: 438 652 2348 438
Size: 214 1696 2259
Fragments arranged by size:
2259 1696 214
NotI GC'GGCC_GC
Cuts at: 581 581
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PmlI CAC'GTG
Cuts at: 765 765
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PpuMI rG'GwC_Cy
Cuts at: 1026 1026
Size: 4169
PspOMI G'GGCC_C
Cuts at: 405 405
Size: 4169
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 886 918 886
Size: 32 4137
PstI C_TGCA'G
Cuts at: 1216 1901 1216
Size: 685 3484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 798 990 1594 1954 798
Size: 192 604 360 3013
Fragments arranged by size:
3013 604 360 192
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SacII CC_GC'GG
Cuts at: 116 609 840 900 1102 116
Size: 493 231 60 202 3183
Fragments arranged by size:
3183 493 231 202 60
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 1212 1897 2890 3081 33
Size: 1179 685 993 191 1121
Fragments arranged by size:
1179 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1067 1067
Size: 4169
SfoI GGC'GCC
Cuts at: 132 384 414 651 1009 1601 1849 132
Size: 252 30 237 358 592 248 2452
Fragments arranged by size:
2452 592 358 252 248 237 30
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SgrAI Cr'CCGG_yG
Cuts at: 331 331
Size: 4169
SmaI CCC'GGG
Cuts at: 326 390 614 1074 1191 326
Size: 64 224 460 117 3304
Fragments arranged by size:
3304 460 224 117 64
SmlI C'TyrA_G
Cuts at: 868 889 1324 2239 2731 2993 868
Size: 21 435 915 492 262 2044
Fragments arranged by size:
2044 915 492 435 262 21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
SrfI GCCC'GGGC
Cuts at: 326 614 326
Size: 288 3881
StyI C'CwwG_G
Cuts at: 107 726 1139 1303 1657 107
Size: 619 413 164 354 2619
Fragments arranged by size:
2619 619 413 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 1885 2344 2553 1752
Size: 133 459 209 3368
Fragments arranged by size:
3368 459 209 133
TatI w'GTAC_w
Cuts at: 455 1031 3684 455
Size: 576 2653 940
Fragments arranged by size:
2653 940 576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 724 1664 150
Size: 574 940 2655
Fragments arranged by size:
2655 940 574
XmaI C'CCGG_G
Cuts at: 324 388 612 1072 1189 324
Size: 64 224 460 117 3304
Fragments arranged by size:
3304 460 224 117 64
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PmeI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PshAI PsiI PspXI
SanDI SexAI SnaBI SspI StuI SwaI XhoI XmnI
ZraI