Restriction map for
pF1KB9832
(Circular) (Six-base) MAPSORT of: pF1KB9832.seq Check: 4545 from: 1 to: 4142
pF1KB9832 4142 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 311 448 1967 2399 3146 3414 283
Size: 28 137 1519 432 747 268 1011
Fragments arranged by size:
1519 1011 747 432 268 137 28
AfeI AGC'GCT
Cuts at: 296 296
Size: 4142
AflIII A'CryG_T
Cuts at: 1494 2598 3575 1494
Size: 1104 977 2061
Fragments arranged by size:
2061 1104 977
AgeI A'CCGG_T
Cuts at: 2530 2530
Size: 4142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 180 1447 3014 180
Size: 1267 1567 1308
Fragments arranged by size:
1567 1308 1267
ApaLI G'TGCA_C
Cuts at: 2912 2912
Size: 4142
ApoI r'AATT_y
Cuts at: 258 686 910 1146 3495 3751 258
Size: 428 224 236 2349 256 649
Fragments arranged by size:
2349 649 428 256 236 224
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 113 1162 113
Size: 1049 3093
BamHI G'GATC_C
Cuts at: 609 822 1167 609
Size: 213 345 3584
Fragments arranged by size:
3584 345 213
BanI G'GyrC_C
Cuts at: 358 1158 1572 1820 1855 358
Size: 800 414 248 35 2645
Fragments arranged by size:
2645 800 414 248 35
BanII G_rGCy'C
Cuts at: 309 933 1074 1156 2186 309
Size: 624 141 82 1030 2265
Fragments arranged by size:
2265 1030 624 141 82
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbvCI CC'TCA_GC
Cuts at: 404 404
Size: 4142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4108
BciVI GTATCCnnnnn_n'
Cuts at: 2040 2801 4142 2040
Size: 761 1341 2040
Fragments arranged by size:
2040 1341 761
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglI GCCn_nnn'nGGC
Cuts at: 202 202
Size: 4142
BglII A'GATC_T
Cuts at: 1657 3694 1657
Size: 2037 2105
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 141 750 887 1767 1860 2916 141
Size: 609 137 880 93 1056 1367
Fragments arranged by size:
1367 1056 880 609 137 93
BmgBI CAC'GTC
Cuts at: 1112 1112
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 727 1354 1435 1770 3914 727
Size: 627 81 335 2144 955
Fragments arranged by size:
2144 955 627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 2509 237
Size: 2272 1870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 982 889
Size: 93 4049
Bpu10I CC'TnA_GC
Cuts at: 404 1133 404
Size: 729 3413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 538 1318 2197 2689 2987 378
Size: 160 780 879 492 298 1533
Fragments arranged by size:
1533 879 780 492 298 160
BsaI GGTCTCn'nnnn_
Cuts at: 3846 3846
Size: 4142
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 1573 1821 2523 4019 1573
Size: 248 702 1496 1696
Fragments arranged by size:
1696 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2804 2951 3362 1331
Size: 521 678 274 147 411 2111
Fragments arranged by size:
2111 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1567 2325 2902 3398 1567
Size: 758 577 496 2311
Fragments arranged by size:
2311 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 874
Size: 4142
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2938 104
Size: 1580 46 1208 1308
Fragments arranged by size:
1580 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 572 933 1156 1934 2124 2916 572
Size: 361 223 778 190 792 1798
Fragments arranged by size:
1798 792 778 361 223 190
Bsp1286I G_dGCh'C
Cuts at: 141 309 572 750 887 933 1074 1156 1767
Size: 168 263 178 137 46 141 82 611
Cuts at: 1767 1860 1934 2124 2186 2916 141
Size: 93 74 190 62 730 1367
Fragments arranged by size:
1367 730 611 263 190 178 168 141
137 93 82 74 62 46
BspEI T'CCGG_A
Cuts at: 3362 3362
Size: 4142
BspHI T'CATG_A
Cuts at: 836 3318 836
Size: 2482 1660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1192 1406 1708 2089 1192
Size: 214 302 381 3245
Fragments arranged by size:
3245 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2054 2054
Size: 4142
BsrFI r'CCGG_y
Cuts at: 2140 2321 2530 2140
Size: 181 209 3752
Fragments arranged by size:
3752 209 181
BssSI C'ACGA_G
Cuts at: 2413 2771 2413
Size: 358 3784
BstAPI GCAn_nnn'nTGC
Cuts at: 459 1519 459
Size: 1060 3082
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstXI CCAn_nnnn'nTGG
Cuts at: 849 849
Size: 4142
BstYI r'GATC_y
Cuts at: 1 177 420 548 609 822 1167 1634 1657
Size: 176 243 128 61 213 345 467 23
Cuts at: 1657 1992 2238 3250 3336 3348 3694 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 345 335 246 243
213 176 128 86 61 23 12
BtgI C'CryG_G
Cuts at: 1085 1085
Size: 4142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 2273 2436 2092
Size: 181 163 3798
Fragments arranged by size:
3798 181 163
BtsI GCAGTG_nn'
Cuts at: 1385 1385
Size: 4142
DraI TTT'AAA
Cuts at: 428 1141 428
Size: 713 3429
DraIII CAC_nnn'GTG
Cuts at: 589 721 589
Size: 132 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1848 2706 1848
Size: 858 3284
EaeI y'GGCC_r
Cuts at: 1727 1901 2292 2319 3664 1727
Size: 174 391 27 1345 2205
Fragments arranged by size:
2205 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 118 749 1013 2165 2375 118
Size: 631 264 1152 210 1885
Fragments arranged by size:
1885 1152 631 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 2660 2806 3952 2350
Size: 310 146 1146 2540
Fragments arranged by size:
2540 1146 310 146
EcoICRI GAG'CTC
Cuts at: 931 1154 931
Size: 223 3919
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 311 448 889 982 1967 2399 3146 3414
Size: 28 137 441 93 985 432 747 268
Cuts at: 3414 283
Size: 1011
Fragments arranged by size:
1011 985 747 441 432 268 137 93
28
EcoO109I rG'GnC_Cy
Cuts at: 1281 1281
Size: 4142
EcoRI G'AATT_C
Cuts at: 910 1146 910
Size: 236 3906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 555 2161 555
Size: 1606 2536
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 298 1576 1824 2846 3825 298
Size: 1278 248 1022 979 615
Fragments arranged by size:
1278 1022 979 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1984 2014 2016 2046 1984
Size: 30 2 30 4080
Fragments arranged by size:
4080 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2813 2997 2813
Size: 184 3958
MscI TGG'CCA
Cuts at: 1903 1903
Size: 4142
MslI CAynn'nnrTG
Cuts at: 542 626 2131 2258 542
Size: 84 1505 127 2426
Fragments arranged by size:
2426 1505 127 84
MspA1I CmG'CkG
Cuts at: 1253 1567 1927 2940 3185 1253
Size: 314 360 1013 245 2210
Fragments arranged by size:
2210 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NcoI C'CATG_G
Cuts at: 1085 1085
Size: 4142
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 1195 1695 2602 1195
Size: 500 907 2735
Fragments arranged by size:
2735 907 500
PciI A'CATG_T
Cuts at: 2598 2598
Size: 4142
PfoI T'CCnGG_A
Cuts at: 959 959
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 4110
PspXI vC'TCGA_Gb
Cuts at: 113 113
Size: 4142
PstI C_TGCA'G
Cuts at: 1065 1189 1874 1065
Size: 124 685 3333
Fragments arranged by size:
3333 685 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 1567 1927 1567
Size: 360 3782
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 933 1156 933
Size: 223 3919
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 2165 2375 2165
Size: 210 3932
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SfcI C'TryA_G
Cuts at: 33 1061 1185 1870 2863 3054 33
Size: 1028 124 685 993 191 1121
Fragments arranged by size:
1121 1028 993 685 191 124
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 113 393 553 1297 2212 2704 2966 113
Size: 280 160 744 915 492 262 1289
Fragments arranged by size:
1289 915 744 492 280 262 160
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 1085 1276 1630 1085
Size: 191 354 3597
Fragments arranged by size:
3597 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 709 775 1725 1858 2317 2526 709
Size: 66 950 133 459 209 2325
Fragments arranged by size:
2325 950 459 209 133 66
TatI w'GTAC_w
Cuts at: 3657 3657
Size: 4142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 269 301 3659 3691 269
Size: 32 3358 32 720
Fragments arranged by size:
3358 720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 1173 1173
Size: 4142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XhoI C'TCGA_G
Cuts at: 113 113
Size: 4142
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
XmnI GAAnn'nnTTC
Cuts at: 296 686 808 296
Size: 390 122 3630
Fragments arranged by size:
3630 390 122
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI