Restriction map for
pF1KB9831
(Circular) (Six-base) MAPSORT of: pF1KB9831.seq Check: 8043 from: 1 to: 4139
pF1KB9831 4139 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 332 1177 332
Size: 845 3294
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1106 1964 2396 3143 3411 800
Size: 306 858 432 747 268 1528
Fragments arranged by size:
1528 858 747 432 306 268
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AleI CACnn'nnGTG
Cuts at: 1087 1087
Size: 4139
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 127
Size: 32 4107
AlwNI CAG_nnn'CTG
Cuts at: 1444 3011 1444
Size: 1567 2572
ApaLI G'TGCA_C
Cuts at: 810 2909 810
Size: 2099 2040
ApoI r'AATT_y
Cuts at: 250 367 904 1143 3492 3748 250
Size: 117 537 239 2349 256 641
Fragments arranged by size:
2349 641 537 256 239 117
AseI AT'TA_AT
Cuts at: 20 920 20
Size: 900 3239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 190 1061 1159 190
Size: 871 98 3170
Fragments arranged by size:
3170 871 98
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 1153 2183 1153
Size: 1030 3109
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 693 693
Size: 4139
BciVI GTATCCnnnnn_n'
Cuts at: 350 1068 2037 2798 4139 350
Size: 718 969 761 1341 350
Fragments arranged by size:
1341 969 761 718 350
BclI T'GATC_A
Cuts at: 308 1659 308
Size: 1351 2788
BglII A'GATC_T
Cuts at: 266 1654 3691 266
Size: 1388 2037 714
Fragments arranged by size:
2037 1388 714
BlpI GC'TnA_GC
Cuts at: 881 1251 881
Size: 370 3769
Bme1580I G_kGCm'C
Cuts at: 593 814 848 1764 1857 2913 593
Size: 221 34 916 93 1056 1819
Fragments arranged by size:
1819 1056 916 221 93 34
BmrI ACTGGGnnnn_n'
Cuts at: 1351 1432 1767 3911 1351
Size: 81 335 2144 1579
Fragments arranged by size:
2144 1579 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 485 606 952 485
Size: 121 346 3672
Fragments arranged by size:
3672 346 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 213 855 1492 213
Size: 642 637 2860
Fragments arranged by size:
2860 642 637
BsaBI GATnn'nnATC
Cuts at: 6 265 929 1653 3690 6
Size: 259 664 724 2037 455
Fragments arranged by size:
2037 724 664 455 259
BsaHI Gr'CG_yC
Cuts at: 434 1570 1818 2520 4016 434
Size: 1136 248 702 1496 557
Fragments arranged by size:
1496 1136 702 557 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 114 114
Size: 4139
BseYI C'CCAG_C
Cuts at: 885 1564 2322 2899 3395 885
Size: 679 758 577 496 1629
Fragments arranged by size:
1629 758 679 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 880 781
Size: 99 4040
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 814 1153 1931 2121 2913 191
Size: 623 339 778 190 792 1417
Fragments arranged by size:
1417 792 778 623 339 190
Bsp1286I G_dGCh'C
Cuts at: 191 593 814 848 1153 1764 1857 1931 2121
Size: 402 221 34 305 611 93 74 190
Cuts at: 2121 2183 2913 191
Size: 62 730 1417
Fragments arranged by size:
1417 730 611 402 305 221 190 93
74 62 34
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 197 2051 197
Size: 1854 2285
BsrFI r'CCGG_y
Cuts at: 756 2137 2318 2527 756
Size: 1381 181 209 2368
Fragments arranged by size:
2368 1381 209 181
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 759 1516 759
Size: 757 3382
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 936 936
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 1114 1114
Size: 4139
BstYI r'GATC_y
Cuts at: 1 266 1109 1164 1631 1654 1989 2235 3247
Size: 265 843 55 467 23 335 246 1012
Cuts at: 3247 3333 3345 3691 1
Size: 86 12 346 449
Fragments arranged by size:
1012 843 467 449 346 335 265 246
86 55 23 12
BtgI C'CryG_G
Cuts at: 107 287 1070 107
Size: 180 783 3176
Fragments arranged by size:
3176 783 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 855 858 855
Size: 3 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 536 1724 1898 2289 2316 3661 536
Size: 1188 174 391 27 1345 1014
Fragments arranged by size:
1345 1188 1014 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 163 307 721 2162 2372 163
Size: 144 414 1441 210 1930
Fragments arranged by size:
1930 1441 414 210 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 2347 2657 2803 3949 307
Size: 2040 310 146 1146 497
Fragments arranged by size:
2040 1146 497 310 146
EcoICRI GAG'CTC
Cuts at: 1151 1151
Size: 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 1106 1964 2396 3143 3411 800
Size: 306 858 432 747 268 1528
Fragments arranged by size:
1528 858 747 432 306 268
EcoO109I rG'GnC_Cy
Cuts at: 1278 1278
Size: 4139
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1042 1920 1042
Size: 878 3261
HaeII r_GCGC'y
Cuts at: 792 799 1573 1821 2843 3822 792
Size: 7 774 248 1022 979 1109
Fragments arranged by size:
1109 1022 979 774 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 140 172 1981 2011 2013 2043 140
Size: 32 1809 30 2 30 2236
Fragments arranged by size:
2236 1809 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2810 2994 2810
Size: 184 3955
MscI TGG'CCA
Cuts at: 538 1900 538
Size: 1362 2777
MslI CAynn'nnrTG
Cuts at: 184 532 1087 2128 2255 184
Size: 348 555 1041 127 2068
Fragments arranged by size:
2068 1041 555 348 127
MspA1I CmG'CkG
Cuts at: 864 1250 1564 1924 2937 3182 864
Size: 386 314 360 1013 245 1821
Fragments arranged by size:
1821 1013 386 360 314 245
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 107 287 107
Size: 180 3959
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 300 1041 1192 1692 2599 300
Size: 741 151 500 907 1840
Fragments arranged by size:
1840 907 741 500 151
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 855 855
Size: 4139
PfoI T'CCnGG_A
Cuts at: 569 893 569
Size: 324 3815
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PmlI CAC'GTG
Cuts at: 855 855
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PsiI TTA'TAA
Cuts at: 446 446
Size: 4139
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 864 1564 1924 864
Size: 700 360 3079
Fragments arranged by size:
3079 700 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 1153 1153
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 307 2162 2372 307
Size: 1855 210 2074
Fragments arranged by size:
2074 1855 210
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SexAI A'CCwGG_T
Cuts at: 725 725
Size: 4139
SfcI C'TryA_G
Cuts at: 33 1032 1182 1867 2860 3051 33
Size: 999 150 685 993 191 1121
Fragments arranged by size:
1121 999 993 685 191 150
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 1063 1161 1063
Size: 98 4041
SmlI C'TyrA_G
Cuts at: 1294 2209 2701 2963 1294
Size: 915 492 262 2470
Fragments arranged by size:
2470 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1041 1192 1692 1041
Size: 151 500 3488
Fragments arranged by size:
3488 500 151
StyI C'CwwG_G
Cuts at: 107 287 521 1273 1627 107
Size: 180 234 752 354 2619
Fragments arranged by size:
2619 752 354 234 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 1722 1855 2314 2523 522
Size: 1200 133 459 209 2138
Fragments arranged by size:
2138 1200 459 209 133
TatI w'GTAC_w
Cuts at: 3654 3654
Size: 4139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XmaI C'CCGG_G
Cuts at: 1061 1159 1061
Size: 98 4041
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI