Restriction map for
pF1KB9827
(Circular) (Six-base) MAPSORT of: pF1KB9827.seq Check: 7027 from: 1 to: 4088
pF1KB9827 4088 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 228 228
Size: 4088
AccI GT'mk_AC
Cuts at: 1126 1126
Size: 4088
Acc65I G'GTAC_C
Cuts at: 1104 1104
Size: 4088
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 307 657 766 888 1913 2345 3092 3360
Size: 87 350 109 122 1025 432 747 268
Cuts at: 3360 220
Size: 948
Fragments arranged by size:
1025 948 747 432 350 268 122 109
87
AflIII A'CryG_T
Cuts at: 1440 2544 3521 1440
Size: 1104 977 2007
Fragments arranged by size:
2007 1104 977
AgeI A'CCGG_T
Cuts at: 2476 2476
Size: 4088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 748 1000 748
Size: 252 3836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 235
Size: 32 4056
AlwNI CAG_nnn'CTG
Cuts at: 387 402 680 744 765 1034 1393 2960 387
Size: 15 278 64 21 269 359 1567 1515
Fragments arranged by size:
1567 1515 359 278 269 64 21 15
ApaLI G'TGCA_C
Cuts at: 916 2858 916
Size: 1942 2146
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AscI GG'CGCG_CC
Cuts at: 519 519
Size: 4088
AseI AT'TA_AT
Cuts at: 20 20
Size: 4088
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 690 1051 1108 690
Size: 361 57 3670
Fragments arranged by size:
3670 361 57
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 124 1104 1518 1766 1801 124
Size: 980 414 248 35 2411
Fragments arranged by size:
2411 980 414 248 35
BanII G_rGCy'C
Cuts at: 1102 2132 1102
Size: 1030 3058
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglI GCCn_nnn'nGGC
Cuts at: 277 505 277
Size: 228 3860
BglII A'GATC_T
Cuts at: 455 1603 3640 455
Size: 1148 2037 903
Fragments arranged by size:
2037 1148 903
BlpI GC'TnA_GC
Cuts at: 937 1200 937
Size: 263 3825
Bme1580I G_kGCm'C
Cuts at: 127 920 1713 1806 2862 127
Size: 793 793 93 1056 1353
Fragments arranged by size:
1353 1056 793 793 93
BmgBI CAC'GTC
Cuts at: 549 927 549
Size: 378 3710
BmrI ACTGGGnnnn_n'
Cuts at: 690 1300 1381 1716 3860 690
Size: 610 81 335 2144 918
Fragments arranged by size:
2144 918 610 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 807 2455 807
Size: 1648 2440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 802 409
Size: 393 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 214 1264 2143 2635 2933 145
Size: 69 1050 879 492 298 1300
Fragments arranged by size:
1300 1050 879 492 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 214 3792 214
Size: 3578 510
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 269 944 1519 1767 2469 3965 269
Size: 675 575 248 702 1496 392
Fragments arranged by size:
1496 702 675 575 392 248
BsaWI w'CCGG_w
Cuts at: 1277 1798 2476 2750 2897 3308 1277
Size: 521 678 274 147 411 2057
Fragments arranged by size:
2057 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 232 406 598 601 604 622 670 148
Size: 84 174 192 3 3 18 48 3566
Fragments arranged by size:
3566 192 174 84 48 18 3 3
BseYI C'CCAG_C
Cuts at: 1034 1513 2271 2848 3344 1034
Size: 479 758 577 496 1778
Fragments arranged by size:
1778 758 577 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 754
Size: 4088
BsiEI CG_ry'CG
Cuts at: 104 509 579 1630 1676 2884 104
Size: 405 70 1051 46 1208 1308
Fragments arranged by size:
1308 1208 1051 405 70 46
BsiHKAI G_wGCw'C
Cuts at: 920 1102 1880 2070 2862 920
Size: 182 778 190 792 2146
Fragments arranged by size:
2146 792 778 190 182
Bsp1286I G_dGCh'C
Cuts at: 127 920 1102 1713 1806 1880 2070 2132 2862
Size: 793 182 611 93 74 190 62 730
Cuts at: 2862 127
Size: 1353
Fragments arranged by size:
1353 793 730 611 190 182 93 74
62
BspEI T'CCGG_A
Cuts at: 3308 3308
Size: 4088
BspHI T'CATG_A
Cuts at: 3264 3264
Size: 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 1138 1352 1654 2035 228
Size: 910 214 302 381 2281
Fragments arranged by size:
2281 910 381 302 214
BsrBI CCG'CTC
Cuts at: 50 363 414 893 900 2380 2434 3918 4083
Size: 313 51 479 7 1480 54 1484 165
Cuts at: 4083 50
Size: 55
Fragments arranged by size:
1484 1480 479 313 165 55 54 51
7
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 596 698 796 2086 2267 2476 596
Size: 102 98 1290 181 209 2208
Fragments arranged by size:
2208 1290 209 181 102 98
BssHII G'CGCG_C
Cuts at: 519 519
Size: 4088
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 145 402 765 1465 145
Size: 257 363 700 2768
Fragments arranged by size:
2768 700 363 257
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstEII G'GTnAC_C
Cuts at: 309 666 309
Size: 357 3731
BstXI CCAn_nnnn'nTGG
Cuts at: 300 300
Size: 4088
BstYI r'GATC_y
Cuts at: 1 455 1113 1580 1603 1938 2184 3196 3282
Size: 454 658 467 23 335 246 1012 86
Cuts at: 3282 3294 3640 1
Size: 12 346 449
Fragments arranged by size:
1012 658 467 454 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 272 950 107
Size: 165 678 3245
Fragments arranged by size:
3245 678 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 855 2038 2219 2382 195
Size: 660 1183 181 163 1901
Fragments arranged by size:
1901 1183 660 181 163
BtsI GCAGTG_nn'
Cuts at: 1331 1331
Size: 4088
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 371 506 1673 1847 2238 2265 3610 371
Size: 135 1167 174 391 27 1345 849
Fragments arranged by size:
1345 1167 849 391 174 135 27
EagI C'GGCC_G
Cuts at: 506 1673 506
Size: 1167 2921
EarI CTCTTCn'nnn_
Cuts at: 907 2111 2321 907
Size: 1204 210 2674
Fragments arranged by size:
2674 1204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 783 2296 2606 2752 3898 783
Size: 1513 310 146 1146 973
Fragments arranged by size:
1513 1146 973 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 307 409 657 766 802 888 1913 2345
Size: 87 102 248 109 36 86 1025 432
Cuts at: 2345 3092 3360 220
Size: 747 268 948
Fragments arranged by size:
1025 948 747 432 268 248 109 102
87 86 36
EcoNI CCTnn'n_nnAGG
Cuts at: 748 748
Size: 4088
EcoO109I rG'GnC_Cy
Cuts at: 342 828 1227 342
Size: 486 399 3203
Fragments arranged by size:
3203 486 399
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1035 2107 1035
Size: 1072 3016
FspI TGC'GCA
Cuts at: 718 769 991 1869 718
Size: 51 222 878 2937
Fragments arranged by size:
2937 878 222 51
HaeII r_GCGC'y
Cuts at: 381 757 1522 1770 2792 3771 381
Size: 376 765 248 1022 979 698
Fragments arranged by size:
1022 979 765 698 376 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1930 1960 1962 1992 274
Size: 32 1624 30 2 30 2370
Fragments arranged by size:
2370 1624 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 1108 1108
Size: 4088
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2759 2943 2759
Size: 184 3904
MscI TGG'CCA
Cuts at: 373 1849 373
Size: 1476 2612
MslI CAynn'nnrTG
Cuts at: 2077 2204 2077
Size: 127 3961
MspA1I CmG'CkG
Cuts at: 180 369 399 420 435 995 1034 1199 1513
Size: 189 30 21 15 560 39 165 314
Cuts at: 1513 1873 2886 3131 180
Size: 360 1013 245 1137
Fragments arranged by size:
1137 1013 560 360 314 245 189 165
39 30 21 15
NaeI GCC'GGC
Cuts at: 798 2269 798
Size: 1471 2617
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 107 950 107
Size: 843 3245
NgoMIV G'CCGG_C
Cuts at: 796 2267 796
Size: 1471 2617
NspI r_CATG'y
Cuts at: 1141 1641 2548 1141
Size: 500 907 2681
Fragments arranged by size:
2681 907 500
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PflMI CCAn_nnn'nTGG
Cuts at: 387 1034 387
Size: 647 3441
PfoI T'CCnGG_A
Cuts at: 779 779
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PpuMI rG'GwC_Cy
Cuts at: 828 828
Size: 4088
PstI C_TGCA'G
Cuts at: 407 761 1135 1820 407
Size: 354 374 685 2675
Fragments arranged by size:
2675 685 374 354
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 369 995 1513 1873 369
Size: 626 518 360 2584
Fragments arranged by size:
2584 626 518 360
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 907 2111 2321 907
Size: 1204 210 2674
Fragments arranged by size:
2674 1204 210
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 403 757 1131 1816 2809 3000 33
Size: 370 354 374 685 993 191 1121
Fragments arranged by size:
1121 993 685 374 370 354 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 505 505
Size: 4088
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SgrAI Cr'CCGG_yG
Cuts at: 698 796 698
Size: 98 3990
SmaI CCC'GGG
Cuts at: 1110 1110
Size: 4088
SmlI C'TyrA_G
Cuts at: 160 229 1243 2158 2650 2912 160
Size: 69 1014 915 492 262 1336
Fragments arranged by size:
1336 1014 915 492 262 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 1141 1641 1141
Size: 500 3588
StuI AGG'CCT
Cuts at: 1042 1042
Size: 4088
StyI C'CwwG_G
Cuts at: 107 950 1222 1576 107
Size: 843 272 354 2619
Fragments arranged by size:
2619 843 354 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1671 1804 2263 2472 1671
Size: 133 459 209 3287
Fragments arranged by size:
3287 459 209 133
TatI w'GTAC_w
Cuts at: 116 1070 3603 116
Size: 954 2533 601
Fragments arranged by size:
2533 954 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3605 3637 3605
Size: 32 4056
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 1108 1108
Size: 4088
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI