Restriction map for
pF1KB9817
(Circular) (Six-base) MAPSORT of: pF1KB9817.seq Check: 8269 from: 1 to: 4028
pF1KB9817 4028 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 437 437
Size: 4028
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1853 2285 3032 3300 565
Size: 1288 432 747 268 1293
Fragments arranged by size:
1293 1288 747 432 268
AflIII A'CryG_T
Cuts at: 932 1380 2484 3461 932
Size: 448 1104 977 1499
Fragments arranged by size:
1499 1104 977 448
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AhdI GACnn_n'nnGTC
Cuts at: 611 611
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 722 722
Size: 4028
AlwNI CAG_nnn'CTG
Cuts at: 984 1333 2900 984
Size: 349 1567 2112
Fragments arranged by size:
2112 1567 349
ApaLI G'TGCA_C
Cuts at: 794 2798 794
Size: 2004 2024
ApoI r'AATT_y
Cuts at: 525 712 1032 3381 3637 525
Size: 187 320 2349 256 916
Fragments arranged by size:
2349 916 320 256 187
AseI AT'TA_AT
Cuts at: 20 563 20
Size: 543 3485
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
AvrII C'CTAG_G
Cuts at: 618 618
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 1044 1458 1706 1741 1044
Size: 414 248 35 3331
Fragments arranged by size:
3331 414 248 35
BanII G_rGCy'C
Cuts at: 1042 2072 1042
Size: 1030 2998
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 761 761
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 798 1653 1746 2802 798
Size: 855 93 1056 2024
Fragments arranged by size:
2024 1056 855 93
BmgBI CAC'GTC
Cuts at: 933 933
Size: 4028
BmrI ACTGGGnnnn_n'
Cuts at: 207 395 1240 1321 1656 3800 207
Size: 188 845 81 335 2144 435
Fragments arranged by size:
2144 845 435 335 188 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 214
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2083 2575 2873 1204
Size: 879 492 298 2359
Fragments arranged by size:
2359 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 658 1381 658
Size: 723 3305
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 798 1042 1820 2010 2802 798
Size: 244 778 190 792 2024
Fragments arranged by size:
2024 792 778 244 190
BsmI GAATG_Cn'
Cuts at: 132 841 132
Size: 709 3319
BsmBI CGTCTCn'nnnn_
Cuts at: 619 619
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 798 1042 1653 1746 1820 2010 2072 2802 798
Size: 244 611 93 74 190 62 730 2024
Fragments arranged by size:
2024 730 611 244 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 1078 1292 1594 1975 437
Size: 641 214 302 381 2490
Fragments arranged by size:
2490 641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BsrGI T'GTAC_A
Cuts at: 433 433
Size: 4028
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 836 1405 836
Size: 569 3459
BstBI TT'CG_AA
Cuts at: 480 2389 480
Size: 1909 2119
BstYI r'GATC_y
Cuts at: 1 1053 1520 1543 1878 2124 3136 3222 3234
Size: 1052 467 23 335 246 1012 86 12
Cuts at: 3234 3580 1
Size: 346 449
Fragments arranged by size:
1052 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 523 1271 523
Size: 748 3280
DraI TTT'AAA
Cuts at: 240 1027 240
Size: 787 3241
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 1613 1787 2178 2205 3550 1613
Size: 174 391 27 1345 2091
Fragments arranged by size:
2091 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 127 2051 2261 127
Size: 1924 210 1894
Fragments arranged by size:
1924 1894 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 565 1853 2285 3032 3300 214
Size: 351 1288 432 747 268 942
Fragments arranged by size:
1288 942 747 432 351 268
EcoO109I rG'GnC_Cy
Cuts at: 189 1167 189
Size: 978 3050
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
EcoRV GAT'ATC
Cuts at: 391 391
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 597 629 1870 1900 1902 1932 296
Size: 32 269 32 1241 30 2 30 2392
Fragments arranged by size:
2392 1241 269 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MfeI C'AATT_G
Cuts at: 437 437
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 146 2699 2883 146
Size: 2553 184 1291
Fragments arranged by size:
2553 1291 184
MscI TGG'CCA
Cuts at: 1789 1789
Size: 4028
MslI CAynn'nnrTG
Cuts at: 2017 2144 2017
Size: 127 3901
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2826 3071 1139
Size: 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 107
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 1081 1581 2488 1081
Size: 500 907 2621
Fragments arranged by size:
2621 907 500
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PstI C_TGCA'G
Cuts at: 980 1075 1760 980
Size: 95 685 3248
Fragments arranged by size:
3248 685 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 976 1071 1756 2749 2940 33
Size: 943 95 685 993 191 1121
Fragments arranged by size:
1121 993 943 685 191 95
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 1183 2098 2590 2852 1183
Size: 915 492 262 2359
Fragments arranged by size:
2359 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
SspI AAT'ATT
Cuts at: 354 354
Size: 4028
StyI C'CwwG_G
Cuts at: 107 618 1162 1516 107
Size: 511 544 354 2619
Fragments arranged by size:
2619 544 511 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 1611 1744 2203 2412 414
Size: 1197 133 459 209 2030
Fragments arranged by size:
2030 1197 459 209 133
TatI w'GTAC_w
Cuts at: 359 433 935 966 3543 359
Size: 74 502 31 2577 844
Fragments arranged by size:
2577 844 502 74 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI BseRI
BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI