Restriction map for
pF1KB9811
(Circular) (Six-base) MAPSORT of: pF1KB9811.seq Check: 8648 from: 1 to: 3857
pF1KB9811 3857 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1682 2114 2861 3129 490
Size: 1192 432 747 268 1218
Fragments arranged by size:
1218 1192 747 432 268
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 514 2245 514
Size: 1731 2126
AlwNI CAG_nnn'CTG
Cuts at: 774 1162 2729 774
Size: 388 1567 1902
Fragments arranged by size:
1902 1567 388
ApaI G_GGCC'C
Cuts at: 344 344
Size: 3857
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 343 667 877 343
Size: 324 210 3323
Fragments arranged by size:
3323 324 210
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 190 873 1287 1535 1570 190
Size: 683 414 248 35 2477
Fragments arranged by size:
2477 683 414 248 35
BanII G_rGCy'C
Cuts at: 215 328 344 383 845 871 1901 215
Size: 113 16 39 462 26 1030 2171
Fragments arranged by size:
2171 1030 462 113 39 26 16
BbeI G_GCGC'C
Cuts at: 194 1291 1539 194
Size: 1097 248 2512
Fragments arranged by size:
2512 1097 248
BbsI GAAGACnn'nnnn_
Cuts at: 453 633 453
Size: 180 3677
BbvCI CC'TCA_GC
Cuts at: 730 730
Size: 3857
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglI GCCn_nnn'nGGC
Cuts at: 142 508 142
Size: 366 3491
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 344 1482 1575 2631 344
Size: 1138 93 1056 1570
Fragments arranged by size:
1570 1138 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1069 1150 1485 3629 1069
Size: 81 335 2144 1297
Fragments arranged by size:
2144 1297 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 231 342 696 2224 231
Size: 111 354 1528 1864
Fragments arranged by size:
1864 1528 354 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 367 303
Size: 64 3793
Bpu10I CC'TnA_GC
Cuts at: 730 730
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1912 2404 2702 1033
Size: 879 492 298 2188
Fragments arranged by size:
2188 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 191 446 1288 1536 2238 3734 191
Size: 255 842 248 702 1496 314
Fragments arranged by size:
1496 842 702 314 255 248
BsaWI w'CCGG_w
Cuts at: 514 1046 1567 2245 2519 2666 3077 514
Size: 532 521 678 274 147 411 1294
Fragments arranged by size:
1294 678 532 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 334 346 685 223
Size: 111 12 339 3395
Fragments arranged by size:
3395 339 111 12
BseYI C'CCAG_C
Cuts at: 405 1282 2040 2617 3113 405
Size: 877 758 577 496 1149
Fragments arranged by size:
1149 877 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 3857
BsiEI CG_ry'CG
Cuts at: 104 803 1399 1445 2653 104
Size: 699 596 46 1208 1308
Fragments arranged by size:
1308 1208 699 596 46
BsiHKAI G_wGCw'C
Cuts at: 215 383 871 1649 1839 2631 215
Size: 168 488 778 190 792 1441
Fragments arranged by size:
1441 792 778 488 190 168
Bsp1286I G_dGCh'C
Cuts at: 215 328 344 383 845 871 1482 1575 1649
Size: 113 16 39 462 26 611 93 74
Cuts at: 1649 1839 1901 2631 215
Size: 190 62 730 1441
Fragments arranged by size:
1441 730 611 462 190 113 93 74
62 39 26 16
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 182 3033 182
Size: 2851 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 907 1121 1423 1804 242
Size: 665 214 302 381 2295
Fragments arranged by size:
2295 665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1769 1769
Size: 3857
BsrFI r'CCGG_y
Cuts at: 514 1855 2036 2245 514
Size: 1341 181 209 2126
Fragments arranged by size:
2126 1341 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 774 1234 774
Size: 460 3397
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstXI CCAn_nnnn'nTGG
Cuts at: 615 684 615
Size: 69 3788
BstYI r'GATC_y
Cuts at: 1 882 1349 1372 1707 1953 2965 3051 3063
Size: 881 467 23 335 246 1012 86 12
Cuts at: 3063 3409 1
Size: 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 194 569 710 107
Size: 87 375 141 3254
Fragments arranged by size:
3254 375 141 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 1100 1100
Size: 3857
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 267 500 509 686 800 1442 1616 2007 2034
Size: 233 9 177 114 642 174 391 27
Cuts at: 2034 3379 267
Size: 1345 745
Fragments arranged by size:
1345 745 642 391 233 177 174 114
27 9
EagI C'GGCC_G
Cuts at: 800 1442 800
Size: 642 3215
EarI CTCTTCn'nnn_
Cuts at: 218 812 1880 2090 218
Size: 594 1068 210 1985
Fragments arranged by size:
1985 1068 594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 601 2065 2375 2521 3667 601
Size: 1464 310 146 1146 791
Fragments arranged by size:
1464 1146 791 310 146
EcoICRI GAG'CTC
Cuts at: 213 381 869 213
Size: 168 488 3201
Fragments arranged by size:
3201 488 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 367 490 1682 2114 2861 3129 303
Size: 64 123 1192 432 747 268 1031
Fragments arranged by size:
1192 1031 747 432 268 123 64
EcoNI CCTnn'n_nnAGG
Cuts at: 430 502 733 430
Size: 72 231 3554
Fragments arranged by size:
3554 231 72
EcoO109I rG'GnC_Cy
Cuts at: 340 996 340
Size: 656 3201
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 1876
Size: 3857
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 194 1291 1539 2561 3540 194
Size: 1097 248 1022 979 511
Fragments arranged by size:
1097 1022 979 511 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 1699 1729 1731 1761 105
Size: 32 1562 30 2 30 2201
Fragments arranged by size:
2201 1562 32 30 30 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 190 1287 1535 190
Size: 1097 248 2512
Fragments arranged by size:
2512 1097 248
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2712 2528
Size: 184 3673
MscI TGG'CCA
Cuts at: 511 688 1618 511
Size: 177 930 2750
Fragments arranged by size:
2750 930 177
MslI CAynn'nnrTG
Cuts at: 1846 1973 1846
Size: 127 3730
MspA1I CmG'CkG
Cuts at: 771 968 1282 1642 2655 2900 771
Size: 197 314 360 1013 245 1728
Fragments arranged by size:
1728 1013 360 314 245 197
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 191 1288 1536 191
Size: 1097 248 2512
Fragments arranged by size:
2512 1097 248
NcoI C'CATG_G
Cuts at: 107 569 710 107
Size: 462 141 3254
Fragments arranged by size:
3254 462 141
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 910 1410 2317 910
Size: 500 907 2450
Fragments arranged by size:
2450 907 500
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PspOMI G'GGCC_C
Cuts at: 340 340
Size: 3857
PstI C_TGCA'G
Cuts at: 904 1589 904
Size: 685 3172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 771 1282 1642 771
Size: 511 360 2986
Fragments arranged by size:
2986 511 360
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 215 383 871 215
Size: 168 488 3201
Fragments arranged by size:
3201 488 168
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 218 1880 2090 218
Size: 1662 210 1985
Fragments arranged by size:
1985 1662 210
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 900 1585 2578 2769 33
Size: 867 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 508 508
Size: 3857
SfoI GGC'GCC
Cuts at: 192 1289 1537 192
Size: 1097 248 2512
Fragments arranged by size:
2512 1097 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SgrAI Cr'CCGG_yG
Cuts at: 514 514
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 1012 1927 2419 2681 1012
Size: 915 492 262 2188
Fragments arranged by size:
2188 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 910 1410 910
Size: 500 3357
StyI C'CwwG_G
Cuts at: 107 569 710 991 1345 107
Size: 462 141 281 354 2619
Fragments arranged by size:
2619 462 354 281 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1440 1573 2032 2241 1440
Size: 133 459 209 3056
Fragments arranged by size:
3056 459 209 133
TatI w'GTAC_w
Cuts at: 3372 3372
Size: 3857
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 3825
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI