(Circular) (Six-base) MAPSORT of: pF1KB9801.seq Check: 5068 from: 1 to: 3668 pF1KB9801 3668 bp With 182 enzymes: SgfI * June 20, 2008 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 518 518 Size: 3668 AccI GT'mk_AC Cuts at: 706 706 Size: 3668 Acc65I G'GTAC_C Cuts at: 684 684 Size: 3668 AclI AA'CG_TT Cuts at: 3508 3508 Size: 3668 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 326 1493 1925 2672 2940 326 Size: 1167 432 747 268 1054 Fragments arranged by size: 1167 1054 747 432 268 AflIII A'CryG_T Cuts at: 1020 2124 3101 1020 Size: 1104 977 1587 Fragments arranged by size: 1587 1104 977 AgeI A'CCGG_T Cuts at: 2056 2056 Size: 3668 AlwNI CAG_nnn'CTG Cuts at: 973 2540 973 Size: 1567 2101 ApaLI G'TGCA_C Cuts at: 2438 2438 Size: 3668 ApoI r'AATT_y Cuts at: 672 3021 3277 672 Size: 2349 256 1063 Fragments arranged by size: 2349 1063 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3668 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3668 AvaI C'yCGr_G Cuts at: 688 688 Size: 3668 BamHI G'GATC_C Cuts at: 693 693 Size: 3668 BanI G'GyrC_C Cuts at: 684 1098 1346 1381 684 Size: 414 248 35 2971 Fragments arranged by size: 2971 414 248 35 BanII G_rGCy'C Cuts at: 682 1712 682 Size: 1030 2638 BbeI G_GCGC'C Cuts at: 1102 1350 1102 Size: 248 3420 BbsI GAAGACnn'nnnn_ Cuts at: 178 379 178 Size: 201 3467 BciVI GTATCCnnnnn_n' Cuts at: 1566 2327 3668 1566 Size: 761 1341 1566 Fragments arranged by size: 1566 1341 761 BclI T'GATC_A Cuts at: 1188 1188 Size: 3668 BglI GCCn_nnn'nGGC Cuts at: 112 584 112 Size: 472 3196 BglII A'GATC_T Cuts at: 1183 3220 1183 Size: 2037 1631 BlpI GC'TnA_GC Cuts at: 780 780 Size: 3668 Bme1580I G_kGCm'C Cuts at: 1293 1386 2442 1293 Size: 93 1056 2519 Fragments arranged by size: 2519 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 880 961 1296 3440 880 Size: 81 335 2144 1108 Fragments arranged by size: 2144 1108 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2035 Size: 3668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 844 1723 2215 2513 844 Size: 879 492 298 1999 Fragments arranged by size: 1999 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 130 3372 130 Size: 3242 426 BsaAI yAC'GTr Cuts at: 1021 1021 Size: 3668 BsaBI GATnn'nnATC Cuts at: 6 1182 3219 6 Size: 1176 2037 455 Fragments arranged by size: 2037 1176 455 BsaHI Gr'CG_yC Cuts at: 526 1099 1347 2049 3545 526 Size: 573 248 702 1496 649 Fragments arranged by size: 1496 702 649 573 248 BsaWI w'CCGG_w Cuts at: 629 657 857 1378 2056 2330 2477 2888 629 Size: 28 200 521 678 274 147 411 1409 Fragments arranged by size: 1409 678 521 411 274 200 147 28 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 416 446 416 Size: 30 3638 BseYI C'CCAG_C Cuts at: 183 1093 1851 2428 2924 183 Size: 910 758 577 496 927 Fragments arranged by size: 927 910 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1210 1256 2464 104 Size: 1106 46 1208 1308 Fragments arranged by size: 1308 1208 1106 46 BsiHKAI G_wGCw'C Cuts at: 254 520 682 1460 1650 2442 254 Size: 266 162 778 190 792 1480 Fragments arranged by size: 1480 792 778 266 190 162 BsmI GAATG_Cn' Cuts at: 428 428 Size: 3668 Bsp1286I G_dGCh'C Cuts at: 254 520 682 1293 1386 1460 1650 1712 2442 Size: 266 162 611 93 74 190 62 730 Cuts at: 2442 254 Size: 1480 Fragments arranged by size: 1480 730 611 266 190 162 93 74 62 BspEI T'CCGG_A Cuts at: 629 2888 629 Size: 2259 1409 BspHI T'CATG_A Cuts at: 2844 2844 Size: 3668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 518 718 932 1234 1615 518 Size: 200 214 302 381 2571 Fragments arranged by size: 2571 381 302 214 200 BsrBI CCG'CTC Cuts at: 50 1960 2014 3498 3663 50 Size: 1910 54 1484 165 55 Fragments arranged by size: 1910 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1580 1580 Size: 3668 BsrFI r'CCGG_y Cuts at: 534 1666 1847 2056 534 Size: 1132 181 209 2146 Fragments arranged by size: 2146 1132 209 181 BsrGI T'GTAC_A Cuts at: 566 566 Size: 3668 BssSI C'ACGA_G Cuts at: 1939 2297 1939 Size: 358 3310 BstAPI GCAn_nnn'nTGC Cuts at: 1045 1045 Size: 3668 BstBI TT'CG_AA Cuts at: 2029 2029 Size: 3668 BstYI r'GATC_y Cuts at: 1 296 626 693 1160 1183 1518 1764 2776 Size: 295 330 67 467 23 335 246 1012 Cuts at: 2776 2862 2874 3220 1 Size: 86 12 346 449 Fragments arranged by size: 1012 467 449 346 335 330 295 246 86 67 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3668 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1618 1799 1962 1618 Size: 181 163 3324 Fragments arranged by size: 3324 181 163 BtsI GCAGTG_nn' Cuts at: 911 911 Size: 3668 DraI TTT'AAA Cuts at: 667 667 Size: 3668 DrdI GACnn_nn'nnGTC Cuts at: 381 1374 2232 381 Size: 993 858 1817 Fragments arranged by size: 1817 993 858 EaeI y'GGCC_r Cuts at: 474 536 1253 1427 1818 1845 3190 474 Size: 62 717 174 391 27 1345 952 Fragments arranged by size: 1345 952 717 391 174 62 27 EagI C'GGCC_G Cuts at: 1253 1253 Size: 3668 EarI CTCTTCn'nnn_ Cuts at: 344 1691 1901 344 Size: 1347 210 2111 Fragments arranged by size: 2111 1347 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1876 2186 2332 3478 1876 Size: 310 146 1146 2066 Fragments arranged by size: 2066 1146 310 146 EcoICRI GAG'CTC Cuts at: 680 680 Size: 3668 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 326 1493 1925 2672 2940 326 Size: 1167 432 747 268 1054 Fragments arranged by size: 1167 1054 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 807 807 Size: 3668 EcoRI G'AATT_C Cuts at: 672 672 Size: 3668 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 291 1687 291 Size: 1396 2272 FspI TGC'GCA Cuts at: 1449 1449 Size: 3668 HaeII r_GCGC'y Cuts at: 1102 1350 2372 3351 1102 Size: 248 1022 979 1419 Fragments arranged by size: 1419 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 416 448 618 650 1510 1540 1542 1572 416 Size: 32 170 32 860 30 2 30 2512 Fragments arranged by size: 2512 860 170 32 32 30 30 2 HincII GTy'rAC Cuts at: 707 707 Size: 3668 KasI G'GCGC_C Cuts at: 1098 1346 1098 Size: 248 3420 KpnI G_GTAC'C Cuts at: 688 688 Size: 3668 MluI A'CGCG_T Cuts at: 3101 3101 Size: 3668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2339 2523 2339 Size: 184 3484 MscI TGG'CCA Cuts at: 476 1429 476 Size: 953 2715 MslI CAynn'nnrTG Cuts at: 1657 1784 1657 Size: 127 3541 MspA1I CmG'CkG Cuts at: 118 779 1093 1453 2466 2711 118 Size: 661 314 360 1013 245 1075 Fragments arranged by size: 1075 1013 661 360 314 245 NaeI GCC'GGC Cuts at: 536 1849 536 Size: 1313 2355 NarI GG'CG_CC Cuts at: 1099 1347 1099 Size: 248 3420 NcoI C'CATG_G Cuts at: 107 107 Size: 3668 NgoMIV G'CCGG_C Cuts at: 534 1847 534 Size: 1313 2355 NspI r_CATG'y Cuts at: 721 1221 2128 721 Size: 500 907 2261 Fragments arranged by size: 2261 907 500 PciI A'CATG_T Cuts at: 2124 2124 Size: 3668 PfoI T'CCnGG_A Cuts at: 434 434 Size: 3668 PmeI GTTT'AAAC Cuts at: 667 667 Size: 3668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2807 2839 2807 Size: 32 3636 PstI C_TGCA'G Cuts at: 123 515 715 1400 123 Size: 392 200 685 2391 Fragments arranged by size: 2391 685 392 200 PvuI CG_AT'CG Cuts at: 104 104 Size: 3668 PvuII CAG'CTG Cuts at: 1093 1453 1093 Size: 360 3308 RsrII CG'GwC_CG Cuts at: 1863 1863 Size: 3668 SacI G_AGCT'C Cuts at: 682 682 Size: 3668 SalI G'TCGA_C Cuts at: 705 705 Size: 3668 SapI GCTCTTCn'nnn_ Cuts at: 1691 1901 1691 Size: 210 3458 SbfI CC_TGCA'GG Cuts at: 715 715 Size: 3668 ScaI AGT'ACT Cuts at: 3185 3185 Size: 3668 SfcI C'TryA_G Cuts at: 33 119 511 711 1396 2389 2580 33 Size: 86 392 200 685 993 191 1121 Fragments arranged by size: 1121 993 685 392 200 191 86 SfoI GGC'GCC Cuts at: 1100 1348 1100 Size: 248 3420 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3668 SmaI CCC'GGG Cuts at: 690 690 Size: 3668 SmlI C'TyrA_G Cuts at: 823 1738 2230 2492 823 Size: 915 492 262 1999 Fragments arranged by size: 1999 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3668 SphI G_CATG'C Cuts at: 721 1221 721 Size: 500 3168 StuI AGG'CCT Cuts at: 334 334 Size: 3668 StyI C'CwwG_G Cuts at: 107 802 1156 107 Size: 695 354 2619 Fragments arranged by size: 2619 695 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 520 1251 1384 1843 2052 520 Size: 731 133 459 209 2136 Fragments arranged by size: 2136 731 459 209 133 TatI w'GTAC_w Cuts at: 566 3183 566 Size: 2617 1051 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3185 3217 3185 Size: 32 3636 Tth111I GACn'n_nGTC Cuts at: 1465 1465 Size: 3668 XbaI T'CTAG_A Cuts at: 699 699 Size: 3668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1163 1163 Size: 3668 XmaI C'CCGG_G Cuts at: 688 688 Size: 3668 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI