Restriction map for pF1KB9801
 (Circular) (Six-base) MAPSORT of: pF1KB9801.seq  Check: 5068  from: 1  to: 3668



pF1KB9801  3668 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    518     518 
   Size:       3668
 
AccI GT'mk_AC

Cuts at:    706     706 
   Size:       3668
 
Acc65I G'GTAC_C

Cuts at:    684     684 
   Size:       3668
 
AclI AA'CG_TT

Cuts at:   3508    3508 
   Size:       3668
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    326    1493    1925    2672    2940     326 
   Size:       1167     432     747     268    1054

  Fragments arranged by size: 

                1167    1054     747     432     268
 
AflIII A'CryG_T

Cuts at:   1020    2124    3101    1020 
   Size:       1104     977    1587

  Fragments arranged by size: 

                1587    1104     977
 
AgeI A'CCGG_T

Cuts at:   2056    2056 
   Size:       3668
 
AlwNI CAG_nnn'CTG

Cuts at:    973    2540     973 
   Size:       1567    2101
 
ApaLI G'TGCA_C

Cuts at:   2438    2438 
   Size:       3668
 
ApoI r'AATT_y

Cuts at:    672    3021    3277     672 
   Size:       2349     256    1063

  Fragments arranged by size: 

                2349    1063     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3668
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3668
 
AvaI C'yCGr_G

Cuts at:    688     688 
   Size:       3668
 
BamHI G'GATC_C

Cuts at:    693     693 
   Size:       3668
 
BanI G'GyrC_C

Cuts at:    684    1098    1346    1381     684 
   Size:        414     248      35    2971

  Fragments arranged by size: 

                2971     414     248      35
 
BanII G_rGCy'C

Cuts at:    682    1712     682 
   Size:       1030    2638
 
BbeI G_GCGC'C

Cuts at:   1102    1350    1102 
   Size:        248    3420
 
BbsI GAAGACnn'nnnn_

Cuts at:    178     379     178 
   Size:        201    3467
 
BciVI GTATCCnnnnn_n'

Cuts at:   1566    2327    3668    1566 
   Size:        761    1341    1566

  Fragments arranged by size: 

                1566    1341     761
 
BclI T'GATC_A

Cuts at:   1188    1188 
   Size:       3668
 
BglI GCCn_nnn'nGGC

Cuts at:    112     584     112 
   Size:        472    3196
 
BglII A'GATC_T

Cuts at:   1183    3220    1183 
   Size:       2037    1631
 
BlpI GC'TnA_GC

Cuts at:    780     780 
   Size:       3668
 
Bme1580I G_kGCm'C

Cuts at:   1293    1386    2442    1293 
   Size:         93    1056    2519

  Fragments arranged by size: 

                2519    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    880     961    1296    3440     880 
   Size:         81     335    2144    1108

  Fragments arranged by size: 

                2144    1108     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2035    2035 
   Size:       3668
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    844    1723    2215    2513     844 
   Size:        879     492     298    1999

  Fragments arranged by size: 

                1999     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    130    3372     130 
   Size:       3242     426
 
BsaAI yAC'GTr

Cuts at:   1021    1021 
   Size:       3668
 
BsaBI GATnn'nnATC

Cuts at:      6    1182    3219       6 
   Size:       1176    2037     455

  Fragments arranged by size: 

                2037    1176     455
 
BsaHI Gr'CG_yC

Cuts at:    526    1099    1347    2049    3545     526 
   Size:        573     248     702    1496     649

  Fragments arranged by size: 

                1496     702     649     573     248
 
BsaWI w'CCGG_w

Cuts at:    629     657     857    1378    2056    2330    2477    2888     629 
   Size:         28     200     521     678     274     147     411    1409

  Fragments arranged by size: 

                1409     678     521     411     274     200     147      28
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    416     446     416 
   Size:         30    3638
 
BseYI C'CCAG_C

Cuts at:    183    1093    1851    2428    2924     183 
   Size:        910     758     577     496     927

  Fragments arranged by size: 

                 927     910     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1210    1256    2464     104 
   Size:       1106      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1106      46
 
BsiHKAI G_wGCw'C

Cuts at:    254     520     682    1460    1650    2442     254 
   Size:        266     162     778     190     792    1480

  Fragments arranged by size: 

                1480     792     778     266     190     162
 
BsmI GAATG_Cn'

Cuts at:    428     428 
   Size:       3668
 
Bsp1286I G_dGCh'C

Cuts at:    254     520     682    1293    1386    1460    1650    1712    2442 
   Size:        266     162     611      93      74     190      62     730

Cuts at:   2442     254 
   Size:       1480

  Fragments arranged by size: 

                1480     730     611     266     190     162      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    629    2888     629 
   Size:       2259    1409
 
BspHI T'CATG_A

Cuts at:   2844    2844 
   Size:       3668
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    518     718     932    1234    1615     518 
   Size:        200     214     302     381    2571

  Fragments arranged by size: 

                2571     381     302     214     200
 
BsrBI CCG'CTC

Cuts at:     50    1960    2014    3498    3663      50 
   Size:       1910      54    1484     165      55

  Fragments arranged by size: 

                1910    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1580    1580 
   Size:       3668
 
BsrFI r'CCGG_y

Cuts at:    534    1666    1847    2056     534 
   Size:       1132     181     209    2146

  Fragments arranged by size: 

                2146    1132     209     181
 
BsrGI T'GTAC_A

Cuts at:    566     566 
   Size:       3668
 
BssSI C'ACGA_G

Cuts at:   1939    2297    1939 
   Size:        358    3310
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1045    1045 
   Size:       3668
 
BstBI TT'CG_AA

Cuts at:   2029    2029 
   Size:       3668
 
BstYI r'GATC_y

Cuts at:      1     296     626     693    1160    1183    1518    1764    2776 
   Size:        295     330      67     467      23     335     246    1012

Cuts at:   2776    2862    2874    3220       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     330     295     246
                  86      67      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3668
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1618    1799    1962    1618 
   Size:        181     163    3324

  Fragments arranged by size: 

                3324     181     163
 
BtsI GCAGTG_nn'

Cuts at:    911     911 
   Size:       3668
 
DraI TTT'AAA

Cuts at:    667     667 
   Size:       3668
 
DrdI GACnn_nn'nnGTC

Cuts at:    381    1374    2232     381 
   Size:        993     858    1817

  Fragments arranged by size: 

                1817     993     858
 
EaeI y'GGCC_r

Cuts at:    474     536    1253    1427    1818    1845    3190     474 
   Size:         62     717     174     391      27    1345     952

  Fragments arranged by size: 

                1345     952     717     391     174      62      27
 
EagI C'GGCC_G

Cuts at:   1253    1253 
   Size:       3668
 
EarI CTCTTCn'nnn_

Cuts at:    344    1691    1901     344 
   Size:       1347     210    2111

  Fragments arranged by size: 

                2111    1347     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1876    2186    2332    3478    1876 
   Size:        310     146    1146    2066

  Fragments arranged by size: 

                2066    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    680     680 
   Size:       3668
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    326    1493    1925    2672    2940     326 
   Size:       1167     432     747     268    1054

  Fragments arranged by size: 

                1167    1054     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    807     807 
   Size:       3668
 
EcoRI G'AATT_C

Cuts at:    672     672 
   Size:       3668
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    291    1687     291 
   Size:       1396    2272
 
FspI TGC'GCA

Cuts at:   1449    1449 
   Size:       3668
 
HaeII r_GCGC'y

Cuts at:   1102    1350    2372    3351    1102 
   Size:        248    1022     979    1419

  Fragments arranged by size: 

                1419    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    416     448     618     650    1510    1540    1542    1572     416 
   Size:         32     170      32     860      30       2      30    2512

  Fragments arranged by size: 

                2512     860     170      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    707     707 
   Size:       3668
 
KasI G'GCGC_C

Cuts at:   1098    1346    1098 
   Size:        248    3420
 
KpnI G_GTAC'C

Cuts at:    688     688 
   Size:       3668
 
MluI A'CGCG_T

Cuts at:   3101    3101 
   Size:       3668
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2339    2523    2339 
   Size:        184    3484
 
MscI TGG'CCA

Cuts at:    476    1429     476 
   Size:        953    2715
 
MslI CAynn'nnrTG

Cuts at:   1657    1784    1657 
   Size:        127    3541
 
MspA1I CmG'CkG

Cuts at:    118     779    1093    1453    2466    2711     118 
   Size:        661     314     360    1013     245    1075

  Fragments arranged by size: 

                1075    1013     661     360     314     245
 
NaeI GCC'GGC

Cuts at:    536    1849     536 
   Size:       1313    2355
 
NarI GG'CG_CC

Cuts at:   1099    1347    1099 
   Size:        248    3420
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3668
 
NgoMIV G'CCGG_C

Cuts at:    534    1847     534 
   Size:       1313    2355
 
NspI r_CATG'y

Cuts at:    721    1221    2128     721 
   Size:        500     907    2261

  Fragments arranged by size: 

                2261     907     500
 
PciI A'CATG_T

Cuts at:   2124    2124 
   Size:       3668
 
PfoI T'CCnGG_A

Cuts at:    434     434 
   Size:       3668
 
PmeI GTTT'AAAC

Cuts at:    667     667 
   Size:       3668
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2807    2839    2807 
   Size:         32    3636
 
PstI C_TGCA'G

Cuts at:    123     515     715    1400     123 
   Size:        392     200     685    2391

  Fragments arranged by size: 

                2391     685     392     200
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3668
 
PvuII CAG'CTG

Cuts at:   1093    1453    1093 
   Size:        360    3308
 
RsrII CG'GwC_CG

Cuts at:   1863    1863 
   Size:       3668
 
SacI G_AGCT'C

Cuts at:    682     682 
   Size:       3668
 
SalI G'TCGA_C

Cuts at:    705     705 
   Size:       3668
 
SapI GCTCTTCn'nnn_

Cuts at:   1691    1901    1691 
   Size:        210    3458
 
SbfI CC_TGCA'GG

Cuts at:    715     715 
   Size:       3668
 
ScaI AGT'ACT

Cuts at:   3185    3185 
   Size:       3668
 
SfcI C'TryA_G

Cuts at:     33     119     511     711    1396    2389    2580      33 
   Size:         86     392     200     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     392     200     191      86
 
SfoI GGC'GCC

Cuts at:   1100    1348    1100 
   Size:        248    3420
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3668
 
SmaI CCC'GGG

Cuts at:    690     690 
   Size:       3668
 
SmlI C'TyrA_G

Cuts at:    823    1738    2230    2492     823 
   Size:        915     492     262    1999

  Fragments arranged by size: 

                1999     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3668
 
SphI G_CATG'C

Cuts at:    721    1221     721 
   Size:        500    3168
 
StuI AGG'CCT

Cuts at:    334     334 
   Size:       3668
 
StyI C'CwwG_G

Cuts at:    107     802    1156     107 
   Size:        695     354    2619

  Fragments arranged by size: 

                2619     695     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    520    1251    1384    1843    2052     520 
   Size:        731     133     459     209    2136

  Fragments arranged by size: 

                2136     731     459     209     133
 
TatI w'GTAC_w

Cuts at:    566    3183     566 
   Size:       2617    1051
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3185    3217    3185 
   Size:         32    3636
 
Tth111I GACn'n_nGTC

Cuts at:   1465    1465 
   Size:       3668
 
XbaI T'CTAG_A

Cuts at:    699     699 
   Size:       3668
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1163    1163 
   Size:       3668
 
XmaI C'CCGG_G

Cuts at:    688     688 
   Size:       3668
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PfoI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I    BseRI     BsgI    BsiWI    BsmBI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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