(Circular) (Six-base) MAPSORT of: pF1KB9793.seq Check: 2614 from: 1 to: 3749 pF1KB9793 3749 bp With 182 enzymes: SgfI * June 20, 2008 10:32 .. AccI GT'mk_AC Cuts at: 787 787 Size: 3749 Acc65I G'GTAC_C Cuts at: 765 765 Size: 3749 AclI AA'CG_TT Cuts at: 3589 3589 Size: 3749 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1574 2006 2753 3021 1574 Size: 432 747 268 2302 Fragments arranged by size: 2302 747 432 268 AflIII A'CryG_T Cuts at: 1101 2205 3182 1101 Size: 1104 977 1668 Fragments arranged by size: 1668 1104 977 AgeI A'CCGG_T Cuts at: 2137 2137 Size: 3749 AleI CACnn'nnGTG Cuts at: 262 262 Size: 3749 AlwNI CAG_nnn'CTG Cuts at: 1054 2621 1054 Size: 1567 2182 ApaI G_GGCC'C Cuts at: 549 549 Size: 3749 ApaLI G'TGCA_C Cuts at: 2519 2519 Size: 3749 ApoI r'AATT_y Cuts at: 753 3102 3358 753 Size: 2349 256 1144 Fragments arranged by size: 2349 1144 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3749 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3749 AvaI C'yCGr_G Cuts at: 769 769 Size: 3749 BamHI G'GATC_C Cuts at: 774 774 Size: 3749 BanI G'GyrC_C Cuts at: 765 1179 1427 1462 765 Size: 414 248 35 3052 Fragments arranged by size: 3052 414 248 35 BanII G_rGCy'C Cuts at: 200 549 625 763 1793 200 Size: 349 76 138 1030 2156 Fragments arranged by size: 2156 1030 349 138 76 BbeI G_GCGC'C Cuts at: 1183 1431 1183 Size: 248 3501 BciVI GTATCCnnnnn_n' Cuts at: 1647 2408 3749 1647 Size: 761 1341 1647 Fragments arranged by size: 1647 1341 761 BclI T'GATC_A Cuts at: 1269 1269 Size: 3749 BglI GCCn_nnn'nGGC Cuts at: 332 332 Size: 3749 BglII A'GATC_T Cuts at: 1264 3301 1264 Size: 2037 1712 BlpI GC'TnA_GC Cuts at: 861 861 Size: 3749 Bme1580I G_kGCm'C Cuts at: 549 1374 1467 2523 549 Size: 825 93 1056 1775 Fragments arranged by size: 1775 1056 825 93 BmrI ACTGGGnnnn_n' Cuts at: 961 1042 1377 3521 961 Size: 81 335 2144 1189 Fragments arranged by size: 2144 1189 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 577 2116 577 Size: 1539 2210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 925 1804 2296 2594 925 Size: 879 492 298 2080 Fragments arranged by size: 2080 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 151 3453 151 Size: 3302 447 BsaAI yAC'GTr Cuts at: 228 1102 228 Size: 874 2875 BsaBI GATnn'nnATC Cuts at: 6 1263 3300 6 Size: 1257 2037 455 Fragments arranged by size: 2037 1257 455 BsaHI Gr'CG_yC Cuts at: 1180 1428 2130 3626 1180 Size: 248 702 1496 1303 Fragments arranged by size: 1496 1303 702 248 BsaWI w'CCGG_w Cuts at: 938 1459 2137 2411 2558 2969 938 Size: 521 678 274 147 411 1718 Fragments arranged by size: 1718 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 549 579 549 Size: 30 3719 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 181 181 Size: 3749 BseYI C'CCAG_C Cuts at: 691 1174 1932 2509 3005 691 Size: 483 758 577 496 1435 Fragments arranged by size: 1435 758 577 496 483 BsiEI CG_ry'CG Cuts at: 104 1291 1337 2545 104 Size: 1187 46 1208 1308 Fragments arranged by size: 1308 1208 1187 46 BsiHKAI G_wGCw'C Cuts at: 625 763 1541 1731 2523 625 Size: 138 778 190 792 1851 Fragments arranged by size: 1851 792 778 190 138 BsmI GAATG_Cn' Cuts at: 585 585 Size: 3749 Bsp1286I G_dGCh'C Cuts at: 200 549 625 763 1374 1467 1541 1731 1793 Size: 349 76 138 611 93 74 190 62 Cuts at: 1793 2523 200 Size: 730 1426 Fragments arranged by size: 1426 730 611 349 190 138 93 76 74 62 BspEI T'CCGG_A Cuts at: 2969 2969 Size: 3749 BspHI T'CATG_A Cuts at: 2925 2925 Size: 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 479 799 1013 1315 1696 479 Size: 320 214 302 381 2532 Fragments arranged by size: 2532 381 320 302 214 BsrBI CCG'CTC Cuts at: 50 2041 2095 3579 3744 50 Size: 1991 54 1484 165 55 Fragments arranged by size: 1991 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1661 1661 Size: 3749 BsrFI r'CCGG_y Cuts at: 1747 1928 2137 1747 Size: 181 209 3359 Fragments arranged by size: 3359 209 181 BssSI C'ACGA_G Cuts at: 2020 2378 2020 Size: 358 3391 BstAPI GCAn_nnn'nTGC Cuts at: 1126 1126 Size: 3749 BstBI TT'CG_AA Cuts at: 2110 2110 Size: 3749 BstYI r'GATC_y Cuts at: 1 774 1241 1264 1599 1845 2857 2943 2955 Size: 773 467 23 335 246 1012 86 12 Cuts at: 2955 3301 1 Size: 346 449 Fragments arranged by size: 1012 773 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 131 161 278 398 539 665 131 Size: 30 117 120 141 126 3215 Fragments arranged by size: 3215 141 126 120 117 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1699 1880 2043 1699 Size: 181 163 3405 Fragments arranged by size: 3405 181 163 BtsI GCAGTG_nn' Cuts at: 391 702 992 391 Size: 311 290 3148 Fragments arranged by size: 3148 311 290 DraI TTT'AAA Cuts at: 748 748 Size: 3749 DrdI GACnn_nn'nnGTC Cuts at: 1455 2313 1455 Size: 858 2891 EaeI y'GGCC_r Cuts at: 378 1334 1508 1899 1926 3271 378 Size: 956 174 391 27 1345 856 Fragments arranged by size: 1345 956 856 391 174 27 EagI C'GGCC_G Cuts at: 1334 1334 Size: 3749 EarI CTCTTCn'nnn_ Cuts at: 1772 1982 1772 Size: 210 3539 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1957 2267 2413 3559 1957 Size: 310 146 1146 2147 Fragments arranged by size: 2147 1146 310 146 EcoICRI GAG'CTC Cuts at: 623 761 623 Size: 138 3611 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1574 2006 2753 3021 1574 Size: 432 747 268 2302 Fragments arranged by size: 2302 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 113 184 468 545 546 888 113 Size: 71 284 77 1 342 2974 Fragments arranged by size: 2974 342 284 77 71 1 EcoRI G'AATT_C Cuts at: 753 753 Size: 3749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 659 1768 659 Size: 1109 2640 FspI TGC'GCA Cuts at: 1530 1530 Size: 3749 HaeII r_GCGC'y Cuts at: 1183 1431 2453 3432 1183 Size: 248 1022 979 1500 Fragments arranged by size: 1500 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1591 1621 1623 1653 1591 Size: 30 2 30 3687 Fragments arranged by size: 3687 30 30 2 HincII GTy'rAC Cuts at: 788 788 Size: 3749 KasI G'GCGC_C Cuts at: 1179 1427 1179 Size: 248 3501 KpnI G_GTAC'C Cuts at: 769 769 Size: 3749 MluI A'CGCG_T Cuts at: 3182 3182 Size: 3749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2420 2604 2420 Size: 184 3565 MscI TGG'CCA Cuts at: 380 1510 380 Size: 1130 2619 MslI CAynn'nnrTG Cuts at: 262 1738 1865 262 Size: 1476 127 2146 Fragments arranged by size: 2146 1476 127 MspA1I CmG'CkG Cuts at: 860 1174 1534 2547 2792 860 Size: 314 360 1013 245 1817 Fragments arranged by size: 1817 1013 360 314 245 NaeI GCC'GGC Cuts at: 1930 1930 Size: 3749 NarI GG'CG_CC Cuts at: 1180 1428 1180 Size: 248 3501 NcoI C'CATG_G Cuts at: 398 539 665 398 Size: 141 126 3482 Fragments arranged by size: 3482 141 126 NgoMIV G'CCGG_C Cuts at: 1928 1928 Size: 3749 NspI r_CATG'y Cuts at: 802 1302 2209 802 Size: 500 907 2342 Fragments arranged by size: 2342 907 500 PasI CC'CwG_GG Cuts at: 285 285 Size: 3749 PciI A'CATG_T Cuts at: 2205 2205 Size: 3749 PflMI CCAn_nnn'nTGG Cuts at: 666 666 Size: 3749 PfoI T'CCnGG_A Cuts at: 186 186 Size: 3749 PmeI GTTT'AAAC Cuts at: 748 748 Size: 3749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2888 2920 2888 Size: 32 3717 PpuMI rG'GwC_Cy Cuts at: 113 184 468 113 Size: 71 284 3394 Fragments arranged by size: 3394 284 71 PspOMI G'GGCC_C Cuts at: 545 545 Size: 3749 PstI C_TGCA'G Cuts at: 796 1481 796 Size: 685 3064 PvuI CG_AT'CG Cuts at: 104 104 Size: 3749 PvuII CAG'CTG Cuts at: 1174 1534 1174 Size: 360 3389 RsrII CG'GwC_CG Cuts at: 1944 1944 Size: 3749 SacI G_AGCT'C Cuts at: 625 763 625 Size: 138 3611 SalI G'TCGA_C Cuts at: 786 786 Size: 3749 SapI GCTCTTCn'nnn_ Cuts at: 1772 1982 1772 Size: 210 3539 SbfI CC_TGCA'GG Cuts at: 796 796 Size: 3749 ScaI AGT'ACT Cuts at: 3266 3266 Size: 3749 SfcI C'TryA_G Cuts at: 33 792 1477 2470 2661 33 Size: 759 685 993 191 1121 Fragments arranged by size: 1121 993 759 685 191 SfoI GGC'GCC Cuts at: 1181 1429 1181 Size: 248 3501 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3749 SmaI CCC'GGG Cuts at: 771 771 Size: 3749 SmlI C'TyrA_G Cuts at: 904 1819 2311 2573 904 Size: 915 492 262 2080 Fragments arranged by size: 2080 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3749 SphI G_CATG'C Cuts at: 802 1302 802 Size: 500 3249 StuI AGG'CCT Cuts at: 322 322 Size: 3749 StyI C'CwwG_G Cuts at: 398 475 539 665 883 1237 398 Size: 77 64 126 218 354 2910 Fragments arranged by size: 2910 354 218 126 77 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1332 1465 1924 2133 1332 Size: 133 459 209 2948 Fragments arranged by size: 2948 459 209 133 TatI w'GTAC_w Cuts at: 3264 3264 Size: 3749 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 549 581 3266 3298 549 Size: 32 2685 32 1000 Fragments arranged by size: 2685 1000 32 32 Tth111I GACn'n_nGTC Cuts at: 1546 1546 Size: 3749 XbaI T'CTAG_A Cuts at: 780 780 Size: 3749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 264 396 1244 264 Size: 132 848 2769 Fragments arranged by size: 2769 848 132 XmaI C'CCGG_G Cuts at: 769 769 Size: 3749 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI