Restriction map for pF1KB9793
 (Circular) (Six-base) MAPSORT of: pF1KB9793.seq  Check: 2614  from: 1  to: 3749



pF1KB9793  3749 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    787     787 
   Size:       3749
 
Acc65I G'GTAC_C

Cuts at:    765     765 
   Size:       3749
 
AclI AA'CG_TT

Cuts at:   3589    3589 
   Size:       3749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1574    2006    2753    3021    1574 
   Size:        432     747     268    2302

  Fragments arranged by size: 

                2302     747     432     268
 
AflIII A'CryG_T

Cuts at:   1101    2205    3182    1101 
   Size:       1104     977    1668

  Fragments arranged by size: 

                1668    1104     977
 
AgeI A'CCGG_T

Cuts at:   2137    2137 
   Size:       3749
 
AleI CACnn'nnGTG

Cuts at:    262     262 
   Size:       3749
 
AlwNI CAG_nnn'CTG

Cuts at:   1054    2621    1054 
   Size:       1567    2182
 
ApaI G_GGCC'C

Cuts at:    549     549 
   Size:       3749
 
ApaLI G'TGCA_C

Cuts at:   2519    2519 
   Size:       3749
 
ApoI r'AATT_y

Cuts at:    753    3102    3358     753 
   Size:       2349     256    1144

  Fragments arranged by size: 

                2349    1144     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3749
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
AvaI C'yCGr_G

Cuts at:    769     769 
   Size:       3749
 
BamHI G'GATC_C

Cuts at:    774     774 
   Size:       3749
 
BanI G'GyrC_C

Cuts at:    765    1179    1427    1462     765 
   Size:        414     248      35    3052

  Fragments arranged by size: 

                3052     414     248      35
 
BanII G_rGCy'C

Cuts at:    200     549     625     763    1793     200 
   Size:        349      76     138    1030    2156

  Fragments arranged by size: 

                2156    1030     349     138      76
 
BbeI G_GCGC'C

Cuts at:   1183    1431    1183 
   Size:        248    3501
 
BciVI GTATCCnnnnn_n'

Cuts at:   1647    2408    3749    1647 
   Size:        761    1341    1647

  Fragments arranged by size: 

                1647    1341     761
 
BclI T'GATC_A

Cuts at:   1269    1269 
   Size:       3749
 
BglI GCCn_nnn'nGGC

Cuts at:    332     332 
   Size:       3749
 
BglII A'GATC_T

Cuts at:   1264    3301    1264 
   Size:       2037    1712
 
BlpI GC'TnA_GC

Cuts at:    861     861 
   Size:       3749
 
Bme1580I G_kGCm'C

Cuts at:    549    1374    1467    2523     549 
   Size:        825      93    1056    1775

  Fragments arranged by size: 

                1775    1056     825      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    961    1042    1377    3521     961 
   Size:         81     335    2144    1189

  Fragments arranged by size: 

                2144    1189     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    577    2116     577 
   Size:       1539    2210
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    925    1804    2296    2594     925 
   Size:        879     492     298    2080

  Fragments arranged by size: 

                2080     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    151    3453     151 
   Size:       3302     447
 
BsaAI yAC'GTr

Cuts at:    228    1102     228 
   Size:        874    2875
 
BsaBI GATnn'nnATC

Cuts at:      6    1263    3300       6 
   Size:       1257    2037     455

  Fragments arranged by size: 

                2037    1257     455
 
BsaHI Gr'CG_yC

Cuts at:   1180    1428    2130    3626    1180 
   Size:        248     702    1496    1303

  Fragments arranged by size: 

                1496    1303     702     248
 
BsaWI w'CCGG_w

Cuts at:    938    1459    2137    2411    2558    2969     938 
   Size:        521     678     274     147     411    1718

  Fragments arranged by size: 

                1718     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    549     579     549 
   Size:         30    3719
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    181     181 
   Size:       3749
 
BseYI C'CCAG_C

Cuts at:    691    1174    1932    2509    3005     691 
   Size:        483     758     577     496    1435

  Fragments arranged by size: 

                1435     758     577     496     483
 
BsiEI CG_ry'CG

Cuts at:    104    1291    1337    2545     104 
   Size:       1187      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1187      46
 
BsiHKAI G_wGCw'C

Cuts at:    625     763    1541    1731    2523     625 
   Size:        138     778     190     792    1851

  Fragments arranged by size: 

                1851     792     778     190     138
 
BsmI GAATG_Cn'

Cuts at:    585     585 
   Size:       3749
 
Bsp1286I G_dGCh'C

Cuts at:    200     549     625     763    1374    1467    1541    1731    1793 
   Size:        349      76     138     611      93      74     190      62

Cuts at:   1793    2523     200 
   Size:        730    1426

  Fragments arranged by size: 

                1426     730     611     349     190     138      93      76
                  74      62
 
BspEI T'CCGG_A

Cuts at:   2969    2969 
   Size:       3749
 
BspHI T'CATG_A

Cuts at:   2925    2925 
   Size:       3749
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    479     799    1013    1315    1696     479 
   Size:        320     214     302     381    2532

  Fragments arranged by size: 

                2532     381     320     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2041    2095    3579    3744      50 
   Size:       1991      54    1484     165      55

  Fragments arranged by size: 

                1991    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1661    1661 
   Size:       3749
 
BsrFI r'CCGG_y

Cuts at:   1747    1928    2137    1747 
   Size:        181     209    3359

  Fragments arranged by size: 

                3359     209     181
 
BssSI C'ACGA_G

Cuts at:   2020    2378    2020 
   Size:        358    3391
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1126    1126 
   Size:       3749
 
BstBI TT'CG_AA

Cuts at:   2110    2110 
   Size:       3749
 
BstYI r'GATC_y

Cuts at:      1     774    1241    1264    1599    1845    2857    2943    2955 
   Size:        773     467      23     335     246    1012      86      12

Cuts at:   2955    3301       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     773     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    131     161     278     398     539     665     131 
   Size:         30     117     120     141     126    3215

  Fragments arranged by size: 

                3215     141     126     120     117      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1699    1880    2043    1699 
   Size:        181     163    3405

  Fragments arranged by size: 

                3405     181     163
 
BtsI GCAGTG_nn'

Cuts at:    391     702     992     391 
   Size:        311     290    3148

  Fragments arranged by size: 

                3148     311     290
 
DraI TTT'AAA

Cuts at:    748     748 
   Size:       3749
 
DrdI GACnn_nn'nnGTC

Cuts at:   1455    2313    1455 
   Size:        858    2891
 
EaeI y'GGCC_r

Cuts at:    378    1334    1508    1899    1926    3271     378 
   Size:        956     174     391      27    1345     856

  Fragments arranged by size: 

                1345     956     856     391     174      27
 
EagI C'GGCC_G

Cuts at:   1334    1334 
   Size:       3749
 
EarI CTCTTCn'nnn_

Cuts at:   1772    1982    1772 
   Size:        210    3539
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1957    2267    2413    3559    1957 
   Size:        310     146    1146    2147

  Fragments arranged by size: 

                2147    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    623     761     623 
   Size:        138    3611
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1574    2006    2753    3021    1574 
   Size:        432     747     268    2302

  Fragments arranged by size: 

                2302     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    113     184     468     545     546     888     113 
   Size:         71     284      77       1     342    2974

  Fragments arranged by size: 

                2974     342     284      77      71       1
 
EcoRI G'AATT_C

Cuts at:    753     753 
   Size:       3749
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    659    1768     659 
   Size:       1109    2640
 
FspI TGC'GCA

Cuts at:   1530    1530 
   Size:       3749
 
HaeII r_GCGC'y

Cuts at:   1183    1431    2453    3432    1183 
   Size:        248    1022     979    1500

  Fragments arranged by size: 

                1500    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1591    1621    1623    1653    1591 
   Size:         30       2      30    3687

  Fragments arranged by size: 

                3687      30      30       2
 
HincII GTy'rAC

Cuts at:    788     788 
   Size:       3749
 
KasI G'GCGC_C

Cuts at:   1179    1427    1179 
   Size:        248    3501
 
KpnI G_GTAC'C

Cuts at:    769     769 
   Size:       3749
 
MluI A'CGCG_T

Cuts at:   3182    3182 
   Size:       3749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2420    2604    2420 
   Size:        184    3565
 
MscI TGG'CCA

Cuts at:    380    1510     380 
   Size:       1130    2619
 
MslI CAynn'nnrTG

Cuts at:    262    1738    1865     262 
   Size:       1476     127    2146

  Fragments arranged by size: 

                2146    1476     127
 
MspA1I CmG'CkG

Cuts at:    860    1174    1534    2547    2792     860 
   Size:        314     360    1013     245    1817

  Fragments arranged by size: 

                1817    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1930    1930 
   Size:       3749
 
NarI GG'CG_CC

Cuts at:   1180    1428    1180 
   Size:        248    3501
 
NcoI C'CATG_G

Cuts at:    398     539     665     398 
   Size:        141     126    3482

  Fragments arranged by size: 

                3482     141     126
 
NgoMIV G'CCGG_C

Cuts at:   1928    1928 
   Size:       3749
 
NspI r_CATG'y

Cuts at:    802    1302    2209     802 
   Size:        500     907    2342

  Fragments arranged by size: 

                2342     907     500
 
PasI CC'CwG_GG

Cuts at:    285     285 
   Size:       3749
 
PciI A'CATG_T

Cuts at:   2205    2205 
   Size:       3749
 
PflMI CCAn_nnn'nTGG

Cuts at:    666     666 
   Size:       3749
 
PfoI T'CCnGG_A

Cuts at:    186     186 
   Size:       3749
 
PmeI GTTT'AAAC

Cuts at:    748     748 
   Size:       3749
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2888    2920    2888 
   Size:         32    3717
 
PpuMI rG'GwC_Cy

Cuts at:    113     184     468     113 
   Size:         71     284    3394

  Fragments arranged by size: 

                3394     284      71
 
PspOMI G'GGCC_C

Cuts at:    545     545 
   Size:       3749
 
PstI C_TGCA'G

Cuts at:    796    1481     796 
   Size:        685    3064
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3749
 
PvuII CAG'CTG

Cuts at:   1174    1534    1174 
   Size:        360    3389
 
RsrII CG'GwC_CG

Cuts at:   1944    1944 
   Size:       3749
 
SacI G_AGCT'C

Cuts at:    625     763     625 
   Size:        138    3611
 
SalI G'TCGA_C

Cuts at:    786     786 
   Size:       3749
 
SapI GCTCTTCn'nnn_

Cuts at:   1772    1982    1772 
   Size:        210    3539
 
SbfI CC_TGCA'GG

Cuts at:    796     796 
   Size:       3749
 
ScaI AGT'ACT

Cuts at:   3266    3266 
   Size:       3749
 
SfcI C'TryA_G

Cuts at:     33     792    1477    2470    2661      33 
   Size:        759     685     993     191    1121

  Fragments arranged by size: 

                1121     993     759     685     191
 
SfoI GGC'GCC

Cuts at:   1181    1429    1181 
   Size:        248    3501
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
SmaI CCC'GGG

Cuts at:    771     771 
   Size:       3749
 
SmlI C'TyrA_G

Cuts at:    904    1819    2311    2573     904 
   Size:        915     492     262    2080

  Fragments arranged by size: 

                2080     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3749
 
SphI G_CATG'C

Cuts at:    802    1302     802 
   Size:        500    3249
 
StuI AGG'CCT

Cuts at:    322     322 
   Size:       3749
 
StyI C'CwwG_G

Cuts at:    398     475     539     665     883    1237     398 
   Size:         77      64     126     218     354    2910

  Fragments arranged by size: 

                2910     354     218     126      77      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1332    1465    1924    2133    1332 
   Size:        133     459     209    2948

  Fragments arranged by size: 

                2948     459     209     133
 
TatI w'GTAC_w

Cuts at:   3264    3264 
   Size:       3749
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    549     581    3266    3298     549 
   Size:         32    2685      32    1000

  Fragments arranged by size: 

                2685    1000      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1546    1546 
   Size:       3749
 
XbaI T'CTAG_A

Cuts at:    780     780 
   Size:       3749
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    264     396    1244     264 
   Size:        132     848    2769

  Fragments arranged by size: 

                2769     848     132
 
XmaI C'CCGG_G

Cuts at:    769     769 
   Size:       3749
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
     BpmI   Bpu10I     BsgI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmlI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
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