Restriction map for
pF1KB9788
(Circular) (Six-base) MAPSORT of: pF1KB9788.seq Check: 3231 from: 1 to: 3698
pF1KB9788 3698 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 563 736 563
Size: 173 3525
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 354 1523 1955 2702 2970 267
Size: 87 1169 432 747 268 995
Fragments arranged by size:
1169 995 747 432 268 87
AfeI AGC'GCT
Cuts at: 391 391
Size: 3698
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 3698
AlwNI CAG_nnn'CTG
Cuts at: 453 590 1003 2570 453
Size: 137 413 1567 1581
Fragments arranged by size:
1581 1567 413 137
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 702 3051 3307 702
Size: 2349 256 1093
Fragments arranged by size:
2349 1093 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 718 718
Size: 3698
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 714 1128 1376 1411 714
Size: 414 248 35 3001
Fragments arranged by size:
3001 414 248 35
BanII G_rGCy'C
Cuts at: 688 712 1742 688
Size: 24 1030 2644
Fragments arranged by size:
2644 1030 24
BbeI G_GCGC'C
Cuts at: 1132 1380 1132
Size: 248 3450
BciVI GTATCCnnnnn_n'
Cuts at: 1596 2357 3698 1596
Size: 761 1341 1596
Fragments arranged by size:
1596 1341 761
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 365 1213 3250 365
Size: 848 2037 813
Fragments arranged by size:
2037 848 813
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 1323 1416 2472 1323
Size: 93 1056 2549
Fragments arranged by size:
2549 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 910 991 1326 3470 910
Size: 81 335 2144 1138
Fragments arranged by size:
2144 1138 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 411 2065 411
Size: 1654 2044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 484
Size: 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 555 874 1753 2245 2543 250
Size: 305 319 879 492 298 1405
Fragments arranged by size:
1405 879 492 319 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 671 3402 460
Size: 211 2731 756
Fragments arranged by size:
2731 756 211
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 1129 1377 2079 3575 1129
Size: 248 702 1496 1252
Fragments arranged by size:
1496 1252 702 248
BsaWI w'CCGG_w
Cuts at: 887 1408 2086 2360 2507 2918 887
Size: 521 678 274 147 411 1667
Fragments arranged by size:
1667 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 255 384 552 255
Size: 129 168 3401
Fragments arranged by size:
3401 168 129
BseYI C'CCAG_C
Cuts at: 505 1123 1881 2458 2954 505
Size: 618 758 577 496 1249
Fragments arranged by size:
1249 758 618 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 509 214
Size: 295 3403
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 712 1490 1680 2472 712
Size: 778 190 792 1938
Fragments arranged by size:
1938 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 688 712 1323 1416 1490 1680 1742 2472 688
Size: 24 611 93 74 190 62 730 1914
Fragments arranged by size:
1914 730 611 190 93 74 62 24
BspEI T'CCGG_A
Cuts at: 2918 2918
Size: 3698
BspHI T'CATG_A
Cuts at: 620 2874 620
Size: 2254 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 471 478 748 962 1264 1645 471
Size: 7 270 214 302 381 2524
Fragments arranged by size:
2524 381 302 270 214 7
BsrBI CCG'CTC
Cuts at: 50 1990 2044 3528 3693 50
Size: 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 1696 1877 2086 1696
Size: 181 209 3308
Fragments arranged by size:
3308 209 181
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstYI r'GATC_y
Cuts at: 1 166 365 723 1190 1213 1548 1794 2806
Size: 165 199 358 467 23 335 246 1012
Cuts at: 2806 2892 2904 3250 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 358 346 335 246 199
165 86 23 12
BtgI C'CryG_G
Cuts at: 107 195 107
Size: 88 3610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 941 941
Size: 3698
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 1283 1457 1848 1875 3220 1283
Size: 174 391 27 1345 1761
Fragments arranged by size:
1761 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 270 382 1721 1931 270
Size: 112 1339 210 2037
Fragments arranged by size:
2037 1339 210 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1906 2216 2362 3508 1906
Size: 310 146 1146 2096
Fragments arranged by size:
2096 1146 310 146
EcoICRI GAG'CTC
Cuts at: 710 710
Size: 3698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 354 484 1523 1955 2702 2970 267
Size: 87 130 1039 432 747 268 995
Fragments arranged by size:
1039 995 747 432 268 130 87
EcoNI CCTnn'n_nnAGG
Cuts at: 537 537
Size: 3698
EcoO109I rG'GnC_Cy
Cuts at: 837 837
Size: 3698
EcoRI G'AATT_C
Cuts at: 702 702
Size: 3698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 393 1132 1380 2402 3381 393
Size: 739 248 1022 979 710
Fragments arranged by size:
1022 979 739 710 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 670 702 1540 1570 1572 1602 670
Size: 32 838 30 2 30 2766
Fragments arranged by size:
2766 838 32 30 30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 1128 1376 1128
Size: 248 3450
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 2369 2553 144
Size: 2225 184 1289
Fragments arranged by size:
2225 1289 184
MscI TGG'CCA
Cuts at: 1459 1459
Size: 3698
MslI CAynn'nnrTG
Cuts at: 220 1687 1814 220
Size: 1467 127 2104
Fragments arranged by size:
2104 1467 127
MspA1I CmG'CkG
Cuts at: 809 1123 1483 2496 2741 809
Size: 314 360 1013 245 1766
Fragments arranged by size:
1766 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1879 1879
Size: 3698
NarI GG'CG_CC
Cuts at: 1129 1377 1129
Size: 248 3450
NcoI C'CATG_G
Cuts at: 107 195 107
Size: 88 3610
NgoMIV G'CCGG_C
Cuts at: 1877 1877
Size: 3698
NspI r_CATG'y
Cuts at: 651 751 1251 2158 651
Size: 100 500 907 2191
Fragments arranged by size:
2191 907 500 100
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PflMI CCAn_nnn'nTGG
Cuts at: 300 300
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PstI C_TGCA'G
Cuts at: 362 482 745 1430 362
Size: 120 263 685 2630
Fragments arranged by size:
2630 685 263 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 1123 1483 1123
Size: 360 3338
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 712 712
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 1721 1931 1721
Size: 210 3488
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SfcI C'TryA_G
Cuts at: 33 153 358 478 741 1426 2419 2610 33
Size: 120 205 120 263 685 993 191 1121
Fragments arranged by size:
1121 993 685 263 205 191 120 120
SfoI GGC'GCC
Cuts at: 1130 1378 1130
Size: 248 3450
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 720 720
Size: 3698
SmlI C'TyrA_G
Cuts at: 265 534 853 1768 2260 2522 265
Size: 269 319 915 492 262 1441
Fragments arranged by size:
1441 915 492 319 269 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
StyI C'CwwG_G
Cuts at: 107 195 218 832 1186 107
Size: 88 23 614 354 2619
Fragments arranged by size:
2619 614 354 88 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 3213 3213
Size: 3698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 1495 1495
Size: 3698
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 433 1193 433
Size: 760 2938
XmaI C'CCGG_G
Cuts at: 718 718
Size: 3698
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI